LOCUS       NZ_CP007611             8513 bp    DNA     circular CON 08-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. YBT-1520 plasmid
            pBMB8513, complete sequence.
ACCESSION   NZ_CP007611
VERSION     NZ_CP007611.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02797740
            Assembly: GCF_000747545.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. YBT-1520
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. YBT-1520
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Chen,B., Fu,Y., Zhang,S., Li,X., Qiang,H., Li,M., Zheng,H., Sun,M.,
            Yu,Z. and He,J.
  TITLE     The Complete Genome Sequence of Bacillus thuringiensis subsp.
            kurstaki Strain YBT-1520
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Chen,B., Fu,Y., Zhang,S., Li,X., Qiang,H., Li,M., Zheng,H., Sun,M.,
            Yu,Z. and He,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) State Key Laboratory of Agricultural
            Microbiology, Huazhong Agricultural University, No. 1 Shizishan
            Street, Wuhan, Hubei 430070, P. R. China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio v. 2.0
            Reference-guided Assembly         :: CP004069.1
            Genome Coverage                   :: 150x
            Sequencing Technology             :: 454; Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000747545.1-RS_2024_04_08
            Annotation Date                   :: 04/08/2024 03:11:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,672
            CDSs (total)                      :: 6,517
            Genes (coding)                    :: 6,079
            CDSs (with protein)               :: 6,079
            Genes (RNA)                       :: 155
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 108
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 438
            CDSs (without protein)            :: 438
            Pseudo Genes (ambiguous residues) :: 0 of 438
            Pseudo Genes (frameshifted)       :: 211 of 438
            Pseudo Genes (incomplete)         :: 245 of 438
            Pseudo Genes (internal stop)      :: 99 of 438
            Pseudo Genes (multiple problems)  :: 93 of 438
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP007611.1.
            Source bacteria is available from Jin He(hejin@mail.hzau.edu.cn)
            and Ziniu Yu(yz41@mail.hzau.edu.cn).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     YBT-1520"
                     /mol_type="genomic DNA"
                     /strain="YBT-1520"
                     /serovar="kurstaki"
                     /db_xref="taxon:570416"
                     /plasmid="pBMB8513"
     gene            418..588
                     /locus_tag="DF16_RS36090"
                     /old_locus_tag="DF16_pBMB8513orf00002"
     CDS             418..588
                     /locus_tag="DF16_RS36090"
                     /old_locus_tag="DF16_pBMB8513orf00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218510.1"
                     /translation="MALSLLGLIVFKKSWLVAEKNREDWRGCIAWQRDMLCSRGEYKKR
                     LFNTWMSTVKS"
                     /name="hypothetical protein"
     gene            939..2144
                     /gene="mobV"
                     /locus_tag="DF16_RS29380"
                     /old_locus_tag="DF16_pBMB8513orf00004"
     CDS             939..2144
                     /gene="mobV"
                     /locus_tag="DF16_RS29380"
                     /old_locus_tag="DF16_pBMB8513orf00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033305.1"
                     /translation="MSKFVICHMQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNY
                     DLIHDKEKVDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRF
                     FEESLRFLKGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKM
                     SLDQAKSVDEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVI
                     TREKDGLYREKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGY
                     VKGSILDRMNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            2614..3660
                     /locus_tag="DF16_RS29385"
                     /old_locus_tag="DF16_pBMB8513orf00006"
     CDS             2614..3660
                     /locus_tag="DF16_RS29385"
                     /old_locus_tag="DF16_pBMB8513orf00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            3664..3861
                     /locus_tag="DF16_RS29390"
                     /old_locus_tag="DF16_pBMB8513orf00007"
     CDS             3664..3861
                     /locus_tag="DF16_RS29390"
                     /old_locus_tag="DF16_pBMB8513orf00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     gene            complement(4730..4993)
                     /locus_tag="DF16_RS29395"
                     /old_locus_tag="DF16_pBMB8513orf00010"
     CDS             complement(4730..4993)
                     /locus_tag="DF16_RS29395"
                     /old_locus_tag="DF16_pBMB8513orf00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            5267..5494
                     /locus_tag="DF16_RS29400"
                     /old_locus_tag="DF16_pBMB8513orf00011"
     CDS             5267..5494
                     /locus_tag="DF16_RS29400"
                     /old_locus_tag="DF16_pBMB8513orf00011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5966..7045
                     /locus_tag="DF16_RS29405"
                     /old_locus_tag="DF16_pBMB8513orf00013"
     CDS             5966..7045
                     /locus_tag="DF16_RS29405"
                     /old_locus_tag="DF16_pBMB8513orf00013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102658.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCNQEYKI
                     HFLFLQALNNKLSTEQLEKVMLDAIPYFEKQNLWKYIQDYTEELAIRFYEEKNKDKSNE
                     YFYKSYKAKRNLLERESLK"
                     /name="RapH N-terminal domain-containing protein"
     gene            7042..7188
                     /locus_tag="DF16_RS36095"
                     /old_locus_tag="DF16_pBMB8513orf00014"
     CDS             7042..7188
                     /locus_tag="DF16_RS36095"
                     /old_locus_tag="DF16_pBMB8513orf00014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000976019.1"
                     /translation="MMKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHG
                     KDI"
                     /name="hypothetical protein"
     gene            7308..7889
                     /locus_tag="DF16_RS29410"
                     /old_locus_tag="DF16_pBMB8513orf00015"
     CDS             7308..7889
                     /locus_tag="DF16_RS29410"
                     /old_locus_tag="DF16_pBMB8513orf00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000723966.1"
                     /translation="MKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAATTN
                     NNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYGNDV
                     GLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWKEAA
                     KIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     gene            7882..8079
                     /locus_tag="DF16_RS29415"
                     /old_locus_tag="DF16_pBMB8513orf00016"
     CDS             7882..8079
                     /locus_tag="DF16_RS29415"
                     /old_locus_tag="DF16_pBMB8513orf00016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     Typing          2590..3642
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          940..2141
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgcaaagtg ggattcggtt tccttatgct ctttttttat ttttgctttt taagggtctt
       61 ataatcgttt ttaagtattt taggatgttg ggtttcttaa gggaactaga agttcctaga
      121 atggattctg agcgttctga ggtgtatttt tctatggatt cgatattgga attgctctag
      181 gaatagttgt cgttttattc tgtggctgtt ccgacagtgg ttctcataaa gaatatgatt
      241 gtatcaatag atttgatgat gtttgaaaaa tagagaatag ttgaagctct tcctgtaaaa
      301 caaaaaaaat tatgtttgta gaggaagtag tcatttcact acaagaaaat tcccatagat
      361 cggtggcgag ttgaaaaatg ttgaaggttg gcgaggacat tttacgagga cgcgggaatg
      421 gctttgtcat tgttggggtt aatagttttt aagaaaagtt ggttagtagc tgaaaaaaat
      481 agggaagatt ggcgaggatg tattgcatgg cagcgggaca tgttatgtag tcgtggtgaa
      541 tataaaaaga gactttttaa tacatggatg tcaactgtaa aaagttagag gtagttgtat
      601 gatgtttgtc caacggtaag cggaacgaag tgagcattaa ggagtggacg aacccactgt
      661 gcccagaacc ctcttggaac tccaaaacgt tagccgtttt ggggatgcca accggcgagg
      721 gagtccctca aggattgagg ggttggggag ttcgattgaa aggggtcaag gggaagagtt
      781 tcccttgtgg gtttgggcaa agcccatgat tttttttgcc atgcttgtta tggctcggcg
      841 gtagccgaaa tgtcccgaag ggcacacgtg tttacgcagt aaactgtagt gtgctactgt
      901 ttacatggaa ctacccccga aaaaaaggag cgaggcccat gtcgaagttc gtgatttgcc
      961 atatgcaaaa atttaagatg agagatgtga aaggattaca aatacataat caacgtgaaa
     1021 aagaaagtca ttccaattca gatattattc aagcacgaac ggaacagaat tatgatctga
     1081 ttcatgacaa agaaaaagtg gattataaga aattaattca aaatcgaatt gataatggtg
     1141 ttgtatctaa tcgtgcaatt cggaaagacg ctgttgtttg ttgttcgttc atgatttcgg
     1201 ctagtccgga ttatatgaat tctttatctc aagtggaaca gagacgattc tttgaagagt
     1261 ctcttcgttt tttgaagggg agatatggag aagagaactt tgtgtatgct tccgtacatg
     1321 ttgatgaaaa aacacctcat atgcatgtgg gtatggttcc agttaatgaa aaacaaaaac
     1381 tatcagcgta ttcatttttt aaaaacaaat cagagtttca tgatttacaa gataaaatct
     1441 atgaacatgt gaaagaaaag ggatttgata tagagcgtgg tgtttctagt gatagaaaac
     1501 atttatcgac gcagcggttt aaagcggtga ctttacaaca agaaatagaa gaactagagc
     1561 aagaaaagaa gggaatagat agtcgtcttc atgatttaaa aatgtcttta gatcaagcaa
     1621 aaagtgtgga tgagatccca gttaaagaaa aaggtgggtt tatacgctct aaaacggtcg
     1681 agattacctc ggaagacttc gaaagtataa aagtattagc taggtcgtcg gaagccctta
     1741 gagaagatgt tagacgcctt aaaaacgaat caggtgtaat tacacgtgaa aaagatgggc
     1801 tgtatagaga gaaatgtatt ttagaggggc aaataacaga attgaaaaga gagaatcgag
     1861 gattgaagga agagaatgat ttctttaaga aaacgttgga acgagtgaaa gagttgtata
     1921 aagaaaagct tcctgaattg gctggggtga ttggttacgt taaggggagt atattggaca
     1981 ggatgaattg caaattttta aaacgatatt ttactagtga tgatgaagtg agaggcgctc
     2041 aaaagttttt gaatcataaa caagagcatg aagaacaaca aaagagatta aaacaagtgc
     2101 gaagtagcca acagaaaaat cgggaccagg gattggagag atagtgtggt tctgtttgag
     2161 ttttttgttt tttagattta tagcgttagc ggaatttgtt ttttataact tgcttaagat
     2221 gagtatgttt ctcagatgtt ttatccgtta aaaaaaatgg ttttgaacga aaaaaaatgc
     2281 ccctgtccac ggagtggatt taggggcatt tttttatgat ttttcgtgtt cgaaaaatcg
     2341 agttttttct ttctttaata cataggtaac tatttcggca acgaaattga tgcctatgta
     2401 ttcttttagc ccttatgtat caagggtttg taaaaaaaaa gacttgcttt ttcaggcgga
     2461 tagatttatc atttttagtg tcgaattaaa aatcataaat accacgagag gagcaagtcc
     2521 aaaaaaagga ttcgtacacc cttttttata tcgaatatga aattcagaaa ttagaaattc
     2581 tgatgttttt cagataggac taatatatca aatatggcaa agaaaaatca agatgaaatc
     2641 actggtaaat tacaacctaa aaagaaacag aatatcaaga tttacgaatt tataaagaag
     2701 aaaatgagtg agagcgggaa agaattgttt aaatcgtgca gtatctttaa tgaatttttg
     2761 gctacaaagg ataaaaagaa gaagaaacga gtaaagggga atgattgtaa aaatcgattt
     2821 tgtccgattt gtgcttggag gaaagcaggg aaggacgcgg tgaaaatcgc aacgatgatg
     2881 gaagcaatta agattgagga aaagaaagag tttctttttt taacgttgac gactccaaat
     2941 attaaagctg atatggtgaa aagtgagatt gatagattta ataaagcttt taaaaagtta
     3001 tttgatagac gtaatattca acgttctatt aagggttata ttagaaagct agaaatgacg
     3061 tatgataaag aacgattcat cactgaagaa atgtataaca acaaaaagag aaaagcatat
     3121 tatgattctc gtggattgaa agtagacgat cataacccga attatgatac atataatcct
     3181 cattttcatg tattgctttg tgttgataaa aattatttta aacgtaaaga gctttatatt
     3241 aagcaagaag aatggttaga aatgtggcga gaagtaacag atatgccaga gattacgcaa
     3301 gtacatattc aaaaagtaga gttaatacga gaaggaaatg cagttgcgga ggttgcgaag
     3361 tattcagcga aagactatga aatgtcggtt agtcaagaag tgtttgacgt gttctattta
     3421 gcattaaaag gtcgtcaatt aattgtttac ggtggattat taaaagatta tgcgaaaaaa
     3481 tatgaagatg gcgaattgga caagtataag agtacagaca agaatgaata ttattataga
     3541 ttaatcgcaa tgtggaataa ggatttaatg aaatttgaac aagagtatca agagttatct
     3601 gagagtgaga agcaagagta taacggtcat ttaattaatg aagtagatgt ggaggcgtag
     3661 tgtatgaaag tagcgggaga tttatattat tattgtcttg gttgtaaaaa gtttcatgaa
     3721 tatgaaaaaa ttgatcataa aggtgtaaat agaaaattat gtttttattg tttcaagatt
     3781 caatctaaaa aaacgaaaat tattggtgat gctgaaggta gaatgcaaat ttgtgagact
     3841 tgtcataaag aattgtttta gttggttgat ttgtgaaaaa agcaaagtca aatgttagaa
     3901 atttttaata agggaactga ctgttatatc aagtcagttc ccttatttta ttgagtctat
     3961 ttttgagtgc agcaagtagc attcaaaagt ttttttatga tttaacaaaa aggagttttt
     4021 cttaaattta aatttcgtga atttgtaaat ttatttttaa agtttatttc gagatatatt
     4081 ttgttctact aattttgttt tactaacgta cgaggtgaaa acttaaagtg tgcgcatgaa
     4141 tttttttagt aatatatatt acgataaaat accgcacgtt ttttgatgat ttttttgaaa
     4201 atatagtttt gagaaattct aaattttcgg ggctgatttt ggatttttta tgtgggattt
     4261 gattttggaa tttgttaaat tggaaatctc gatttttcga ttttcggaat ttttgttttc
     4321 tcttttcttt gttattctgt atttcgaaat taaggagttt atataatacc ttcatgacga
     4381 actgaaaacg aaaaaacttt gctcgcatat ttaattcata gatatttcat atcgttaaaa
     4441 agttgcaccg tttcaaacga gttgtgtgat aaaaagatgt ttagattatg tttgaaattg
     4501 ttgtttctgt gaatttttta tttcgcgatt tctgtatatt tttaatcctt tttactctta
     4561 aaatcttttt gcttttgtgc gcaaatgaaa aaatcaaaaa gtcacgcgta acttttatta
     4621 aatatataaa tatattatta aaatacctaa cccttttatg atgaaaaaat ataaatagtt
     4681 aaagaaaaaa gactgataaa aaatatcagt cttttttctt tagttttatt tataaagagt
     4741 atctctttat ggtgttttct actttttctc tccagttctc tatatcgtta attgatattc
     4801 cgatctttat taattcgttt ggaatgaaac tgttatctaa aatccataaa gtgagccttt
     4861 ctaatgtgat tctttcgttt ccaattgaat aaatacgttc actttgtttg caagtatatg
     4921 gttttttacc aacatatttt tctacataaa agtatctttt tccgtttgct ttgtaatttg
     4981 ttaggaatgc caaaatgata atctcctctt agttttttta gagttaaaaa tacattttaa
     5041 aagattaaat tttaagttat atatttaatt ttaactctaa ggggttaaaa tttcaattaa
     5101 aacaaataaa attaaataat aaaacaatat attttatttt taaggttaat attaaatgtg
     5161 aatatataaa aaataacctt aatattattt aatttatgtt ttatttgtat aaaagtatcc
     5221 ttgtaagtaa aatggtatgt acattgaatg aaaaggagta tgaaaaatga aggtagtaag
     5281 taatattcgt atgattatgg cgaaaaagaa tatagataat atttcggatt tggtgagaat
     5341 aacgggagta agtcgtaatt ctattaataa gttgtggcac aatgagaatg tgtcttcttt
     5401 aaaattagat acattgataa cgatctgtga gaagttggat gttgagttat tggatttaat
     5461 tgaatatatt cgagatgata gtgagacaaa ataatttagg ggctatttta gctttagata
     5521 gcttgtgtat gatgaaagca tatttttgct aatcgacaat gagttatatt cacatatgtt
     5581 ttttttgtag ggctatatgt gtatttaaag ttttgatttc tatttgttat tgtatgaata
     5641 tggtttgaat gtggtaaatg aaattatagt cttgctaggt gcgtcaacac cttggaagat
     5701 gagcggtact ggcatacttc gtgatttcat tgatagtagt gcagtaaagt gatggaattg
     5761 atgagtattg aaaaagagtt cctgtttggg ggctcttttt ttatttgtta ctaagtattt
     5821 ttaaatagtg aaggtgctac actatttaaa aatacagaat attaaaaact ttatatatca
     5881 actgttgaaa agttagtctt atacaatagc atgataacat gatagaaaat aaaatataga
     5941 taattttgga aaaggagaag attctatgaa tgttcaacta ggaaacgaac aaattacaaa
     6001 attattaaat gattggtatc aagaaattcg agcgcaacac attattaaag caaaacaatt
     6061 aaaacaaaag attgaaaaag aaattaacaa catagaagaa gaccaaaatt tacttttata
     6121 ttattcactt attgatttga gatacaagat tcttacaaac gactatgcag atagagaaca
     6181 atctttagaa aaaatcgaac aattaaaaga acacacaaat aattttttag attactatta
     6241 tcatttcttt aaaggcatgc atgcaatgaa aacaggaaat tatagtgaaa ctcaaaaata
     6301 ttatgatata gcagaaaaat tactagaaga aattcctgat gaagtagaaa aagctgaatt
     6361 taattatgct gttgcaactt tctattatca tactcatcaa gccttattag caactcaata
     6421 tgctaacaaa gccaaaagtt tttttagtgg gaaactagga tacgagatta aaaccggagc
     6481 atgtgaaaac acacttggta tggcttgtat tacattaagg gaattttctg ttgcagaaga
     6541 atacttgctt tccgcaatta ataaatttga aaaacacagt gaaaacaaat tagctttaat
     6601 cgtacgttat aacttaggtc ttttatatgc agatcaaaat ctttctgaac ttgcaatccg
     6661 ttatcttctt gaatcctttg aaaatcaaga agacgattac aaaacaatgt ttttattagc
     6721 acaagaatat tacaagttaa aacaaacaaa tacagtaaac acatatattg aaaaagggct
     6781 aaatgtttgt aaccaagaat ataaaattca tttcttattt ttacaagcat taaacaataa
     6841 attatctact gaacaattag aaaaagtcat gttagacgcg attccatatt ttgaaaaaca
     6901 aaatctatgg aaatacatac aagattatac tgaagaattg gctataagat tttatgaaga
     6961 aaagaataag gataaatcta atgaatattt ttataaaagt tacaaagcta aaagaaactt
     7021 attagaaagg gagtcattga aatgatgaaa aaatttagtc tgatactaat aggggtagca
     7081 tgtacaacag gtattttctt ttcccaattc aataattcca ttcaaacaca tgatgctaaa
     7141 gaaaaaaatg atattattca acaatatgca catggaaaag atatttaaaa gcaaattcca
     7201 acaattagca atcgacctaa atattaggat tattaacaaa atattgaatt atatgtaaat
     7261 taagtttatt atctattttg aataataaac ttaaggggat gaaatttatg aaaaaaatta
     7321 gtattgagag aagtaattgg agaaaaacat ataaaaaaat gattccagta ttgttagtcg
     7381 gaggaatatt ggcaggaaat gtagcatttg tgacaaataa tgtttttgcg gcaactacaa
     7441 ataacaacaa tataatacaa acgaaacagt ctctcgtttc tggtcaagat gaaaagagca
     7501 ttaatgaatt agcagaagcg tttcaattta tgcatgaaga agccgctata tataataatc
     7561 aaggtgaaat tgttgatttt gattttaata aaatagaagc gaaatatggg aatgatgttg
     7621 gtttagatgc acttaaacaa caaattaaaa aagataaaag tgatattgct ggtttatctt
     7681 ttacaagttg catgaaagat gctttatatg acttttttgg agtaaatgct gtacaagccg
     7741 catttaatgg tggattggca tattatcttg agaaaaaatt atggaaagaa gcggctaaaa
     7801 taggcgttaa attctttata ggaagtagcg tagcaggttt agcggctact ttagcttatt
     7861 atggaggtaa gtgtgcaatt tatggataaa agttcaattt taaaacaaat gtattttatt
     7921 ttatttccgg taacactttt tctatctcta aatatttatc aatggattgt taaaagtgat
     7981 tactcattta caggatatat aatccaaaat ttaattattg tagcattagc gttagtagca
     8041 gctatcatag cttatttaat tactaaaaaa aatatttaac acataatgtt agtctaaata
     8101 acagaaaatt ctaaccctta ttcttatttt gtattatact ggttatgtac aggtaaaatg
     8161 gtttacaagt taatttttcc tcgtttcaaa aaaagtccat tcttttcaaa agaatggact
     8221 tttttcttgt tatatttgct gtaactatta aaagggcatc aaaacgatct acaaaagcct
     8281 caagacgacc cacaaaccat ctttaaacgg tctcaaaacg aactacaacc cgaaaaaacg
     8341 catcccagtt tttttaggtt taattttaag cgcataccaa gcgcatatag gcgcatacgg
     8401 gacacataca aagtgccgaa aaacgcatat aggtcgcata caaacggtag aaatacatcc
     8461 ctaaaaatag agtgatagaa acccaatagg cacagcctat ttcaccactt ttt
//
