LOCUS       NZ_CP006637             2014 bp    DNA     circular CON 07-MAY-2024
DEFINITION  Escherichia coli PCN061 plasmid PCN061p1, complete sequence.
ACCESSION   NZ_CP006637
VERSION     NZ_CP006637.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN03765122
            Assembly: GCF_001029125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli PCN061
  ORGANISM  Escherichia coli PCN061
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Liu,C., Zheng,H., Yang,M., Xu,Z., Wang,X., Wei,L., Tang,B., Liu,F.,
            Zhang,Y., Ding,Y., Tang,X., Wu,B., Johnson,T.J., Chen,H. and Tan,C.
  TITLE     Genome analysis and in vivo virulence of porcine extraintestinal
            pathogenic Escherichia coli strain PCN033
  JOURNAL   BMC Genomics 16 (1), 717 (2015)
   PUBMED   26391348
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Liu,C., Tan,C., Xu,Z. and Chen,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2013) Division of Animal Infectious
            Disease,College of Veterinary Medicine, Huazhong Agricultural
            University, Shizishan Street 1, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.3
            Genome Coverage                   :: 33X
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001029125.1-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 02:04:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,868
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,466
            CDSs (with protein)               :: 4,466
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 285
            CDSs (without protein)            :: 285
            Pseudo Genes (ambiguous residues) :: 0 of 285
            Pseudo Genes (frameshifted)       :: 81 of 285
            Pseudo Genes (incomplete)         :: 191 of 285
            Pseudo Genes (internal stop)      :: 53 of 285
            Pseudo Genes (multiple problems)  :: 40 of 285
            Pseudo Genes (short protein)      :: 5 of 285
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP006637.1.
            Source DNA is available from Chen Tan, email:
            tanchen@mail.hzau.edu.cn.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Escherichia coli PCN061"
                     /mol_type="genomic DNA"
                     /strain="PCN061"
                     /db_xref="taxon:1358422"
                     /plasmid="PCN061p1"
     gene            644..1123
                     /locus_tag="PCN061_RS23160"
                     /old_locus_tag="PCN061_p13"
     CDS             644..1123
                     /locus_tag="PCN061_RS23160"
                     /old_locus_tag="PCN061_p13"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017896843.1"
                     /translation="MRKTLLLCIPFFLLAGCAQTKPQMPDANYEKFAKVEVASDACLNS
                     NFITAQEAGQAHSNISLFLSSWTYDYVRYSNLLAQGHQDVKKIKITQEGCNLLRAKIYQ
                     DTIEAQRYEKQMEIAVQQKAIADQQALQSIQNMQSTSPKTTYCNQIGTQTICNSY"
                     /name="hypothetical protein"
     Typing          34..543
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.743"
                     /coverage="99.0"
                     /evalue="5.94e-177"
ORIGIN
        1 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggacttaaa
       61 gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt
      121 ttaccggtgt cattccgctg ttatggccgc gtttatctca ttccacgcct gacactcagt
      181 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
      241 tgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca cgacaaaacg
      301 ccactggcag cagccactgg taacaggttt gtgcgagaga tgtgttcaga gttcttgaag
      361 tggcggcctg actgaggcta cactagaagg acagatttgg tgacatgtct cgcacaagac
      421 tgttaccacg gttagagaat ctgccaggat taactaacct tcgaaaaacc acctgccagg
      481 gtggtttttt cgtttacaga gcaagagatt acgctctgcc ataaatgatc tcaagaagat
      541 ttaggttatt aagtaggacg aagatcgcta tgctccccgt gattttttat ataccaacca
      601 cggggcatta gttttttctt gtcaaaaagg gataaaaaac tcaatgagaa aaacattatt
      661 actttgcatt ccgttttttt tacttgctgg ttgcgctcaa acaaaaccgc aaatgccaga
      721 tgctaattac gaaaaattcg caaaagttga ggttgcctct gacgcctgct taaactccaa
      781 cttcattaca gcacaggaag ccggtcaggc acattccaac atatccttgt tcttaagttc
      841 atggacatat gactacgttc gttactcaaa cctcttggcg caaggacatc aagatgtaaa
      901 aaaaatcaag ataacacagg aaggctgcaa ccttttgcgc gcaaaaatct atcaagacac
      961 catcgaggca cagagatatg aaaaacagat ggagatagca gtacaacaaa aagccatagc
     1021 agatcagcag gcgttgcagt cgattcaaaa tatgcaaagc acatcaccta aaacaactta
     1081 ctgtaaccaa attggcactc aaacaatctg caacagctac tgatcaaaaa ctgatttacc
     1141 ataacatccc ttgcccgcac cccctgagag gccctcagcg cgtttctgcc gcgtcctctg
     1201 cgtttccggc acctttccct gtccatatcg tgaaaaaacg cgcctgatga tttctgcgcg
     1261 cctgcatggc tatgcacaga agtgaatggc ggccggtttc ggacgaatgc tggcactaaa
     1321 ggcgaatttt gaacaaaaaa aacggacatc gagggtcagg atgtccgaca aaatttgcac
     1381 aaacagtcag ccggtaccga tcagagagcc ggcaaataag tatgacagta aaaagtggtt
     1441 atcaactgcc cccgcgctgc tgattgcgca gttttagggc tttggccagt gcaggcaggc
     1501 ccggccggaa tcaggggatc tggtaacggt gaacgggtgc acagttgact gtgcggccgt
     1561 gaggcgttgg ccaagccgcc aggtgcggca gcttccggtg ggtacaaacg ccagcacttt
     1621 gcgaatcctg ccctagatct caacagcaga gaaatttcgt acgcacgaaa ctcagcgtat
     1681 cgggcggcgc ctgatgaaac attctggagt gtcggcggta ctccaaaaac acatcttgtc
     1741 ccgctgattt tgtcactggc gcaaagcggc ggtaatgaca agacatttcc tgtccgcatc
     1801 aatacgtgca gaaaacgtaa gtggtgaaag atgatagtac ggagcgacag gagcctaagc
     1861 agccaccgga gaaattacgc ttcctcggtc attcgctcac tgcgttcggt catgactctg
     1921 cggcgagcgt tacgggatca atcaaagggc gtggaggtat gaagtgtgag aagagactta
     1981 agggctgccg gttttccata ggctccgccc ccct
//
