LOCUS       NZ_CP006634             4086 bp    DNA     circular CON 07-MAY-2024
DEFINITION  Escherichia coli PCN033 plasmid p2PCN033, complete sequence.
ACCESSION   NZ_CP006634
VERSION     NZ_CP006634.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02469619
            Assembly: GCF_000219515.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli PCN033
  ORGANISM  Escherichia coli PCN033
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Liu,C., Zheng,H., Yang,M., Xu,Z., Wang,X., Wei,L., Tang,B., Liu,F.,
            Zhang,Y., Ding,Y., Tang,X., Wu,B., Johnson,T.J., Chen,H. and Tan,C.
  TITLE     Genome analysis and in vivo virulence of porcine extraintestinal
            pathogenic Escherichia coli strain PCN033
  JOURNAL   BMC Genomics 16 (1), 717 (2015)
   PUBMED   26391348
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Liu,C., Tan,C., Xu,Z. and Chen,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2013) Division of Animal Infectious
            Disease,College of Veterinary Medicine, Huazhong Agricultural
            University, Shizishan Street 1, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.3
            Genome Coverage                   :: 30X
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000219515.2-RS_2024_05_06
            Annotation Date                   :: 05/06/2024 07:49:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,005
            CDSs (total)                      :: 4,885
            Genes (coding)                    :: 4,706
            CDSs (with protein)               :: 4,706
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 68 of 179
            Pseudo Genes (incomplete)         :: 110 of 179
            Pseudo Genes (internal stop)      :: 32 of 179
            Pseudo Genes (multiple problems)  :: 28 of 179
            Pseudo Genes (short protein)      :: 4 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP006634.1.
            Source DNA is available from Chen Tan, email:
            tanchen@mail.hzau.edu.cn.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Escherichia coli PCN033"
                     /mol_type="genomic DNA"
                     /strain="PCN033"
                     /db_xref="taxon:1001989"
                     /plasmid="p2PCN033"
     gene            43..744
                     /locus_tag="PPECC33_RS24570"
                     /old_locus_tag="PPECC33_p21"
     CDS             43..744
                     /locus_tag="PPECC33_RS24570"
                     /old_locus_tag="PPECC33_p21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1000..3159)
                     /gene="mobQ"
                     /locus_tag="PPECC33_RS24575"
                     /old_locus_tag="PPECC33_p23"
     CDS             complement(1000..3159)
                     /gene="mobQ"
                     /locus_tag="PPECC33_RS24575"
                     /old_locus_tag="PPECC33_p23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            3338..3592
                     /gene="mobC"
                     /locus_tag="PPECC33_RS24580"
                     /old_locus_tag="PPECC33_p24"
     CDS             3338..3592
                     /gene="mobC"
                     /locus_tag="PPECC33_RS24580"
                     /old_locus_tag="PPECC33_p24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2802..3160)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3161..3337
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          44..744
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1004..3246)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtttatct tttattcatt accttttggg gagggatgct ctatgtcgga tctaattgcc
       61 tataaatcca atgcgctggt tgaagccagt tataaattga ccctacagga acagcgattt
      121 ttgttacttt gcatcggtcg attgaagtct ggggctgatg cagaatctcc aaaattgcaa
      181 aagacaatga ccattacagc tgcggaatat ttcgattcat tccctgacat ggggcggaaa
      241 aatgccgagg ttcagttaca ggaagcaatt gatcgtttgt gggacaggtc aatcattctc
      301 aaggatgacg agaaacgtga ggagttccgc tggatccagt atcgggcaca gtacgctaaa
      361 ggcgaggcca gagcacaaat aacattttct gacgcagtaa tgccttattt gacacaacta
      421 caagggcaat ttactagagt tgtaatcaag aatatatcta gattatcaag aagttacagt
      481 ataaggatat acgaactcct ccagcagttc cgcagtactg gcgaacgtat tatcgctctc
      541 gatgattttc gctcaatgct tgatatagag cataaatatc aaacatataa atcacttaac
      601 cagcaattat taagaccctg cattgacgaa ctgaacaaaa aaagcgacct ggctgtaacg
      661 gttgagacga tcaaaaaggg gcgcacggtt gtagccctgc attttcgctt caaggaagac
      721 aaacaaatca agatgacgat ctaaaatcga ttttaaggca cctaaaacgc tccctgctgc
      781 gttttttttg tattagtagt gcgaaggtac tattgaagtg aaaaattgcg tcaaaagcgc
      841 tctaatgcag cgaaaaaaga accccctttt tttgggggcg gaaaaacaaa actatgcttg
      901 cattatgtgt gtgcttcttg cattcatccc tgtcgatgaa catcgaagaa catcgacgaa
      961 cataacacga tgttaaacaa aacaaaatat tgttcacatt cactgtgacc aaccgcgatc
     1021 atgtctcctc tcggtggcgt gtgttcgtgc atcttcctcg cgccgccatt cgtcccaggt
     1081 acgattaaaa cccagcgagc ggaacagatg atccggctgt ttttctggtg tgttgacggc
     1141 agcaaaaagc ggtgcctcac gggttttcag atgaaactcc ataagttccg gcgcaggtga
     1201 tatgtggctg tctgcatcgt gatttacggc aacaccatac cctgcatacc gtaacccctt
     1261 atcggtcagt gcactgatga ccagctggct gttatcggct gagggaacgg caaacgcggg
     1321 atacagcgta ccattaagcc ggacaacacc gcccagagag tcatccacgc ccatgaccag
     1381 caaaagctga agctggcggg tttcccggtc gacagaactc attaccggca tcagcgtcgg
     1441 tagcagacgt ccctgcgtaa aatccacctt ccttgctgcc agtgatggca tggctggctg
     1501 gcggtcttct ccaagacaga aagactccgg ccatgtcttc ggctggcgga gattgcgggt
     1561 atattgcggc catgtctggc aaacctgcgt cagaagttgc tcaaaggtcg cagaggaata
     1621 aacacgttgc agtggtggat gttgaccgat ttcgcttcgg tcacagatat aaccactcct
     1681 gtcacgcatt aaatcaacac gctcaaagat tctggtgcgg tatttctcca gctcgttccc
     1741 ccagagcgtg tattctccgt tccggttgtg aagcagaagg taacaggtca tttccccgtt
     1801 accaggcatg ggggcctcgc cataccccgt aacgactgcg ctcagtaccc ctgaatccat
     1861 aacggcattg tcatttttcc tgagctgttc cgctcgtcgc tccgcgcgac tacgttctgt
     1921 atcctcctgc tccttccggc gcaattcctc ttcacgttta agcaactgga ctctgagagc
     1981 tgcaaggccg gatgcccccc actgctgcca ctgtttttca aatgccgtct gactcgcggg
     2041 aatgtcctgt ttctccacct tccggtaatg ctgtcgcaat ttttccattt gctggctcag
     2101 cgactggatt tttccctgtt cgctacggta ttccggtgcc gcccattgca tcaggggaat
     2161 acgggaaaaa ccggacgaaa acggcgatga ttttttcagg ctggcagcat ggctcatcgc
     2221 ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc gtcgcctgca tatctgccat
     2281 catcgacatg gcattactgg tgagtttcca tgccgcatta ctgtccttcg gcaacgcttc
     2341 gatgatgtca cgtagtgtgt acgaagttgc gttgtcagga agcacgatat tcagaaggtc
     2401 agccacacgg cgggcaggct ctgtcagccc gctgtttctg agttcggtac gggaaatctg
     2461 tatttccagg ttaagtctgt taatcagacg attctgttcg ccgcgttctg tttcgatacc
     2521 gcgtgtttcc atcgcacagg cagactttcc gaggtgaata ccaggcgttt tctcaagccc
     2581 ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa ccggcgttcg ccagtgccct
     2641 gttcgcatgg tcagcccatg atgcacgcca cgtctcggca tgtgtccggt cgttccagtc
     2701 cctgaccttt ccaccgaatc cagcaggtcc gacagctctc gtggtcagca tgatgtgcgc
     2761 atggggattt gttttatcca tgtgatggaa cgccacatca gcaatcatgc cctgactgac
     2821 aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg gcatcctgcg gtagttcgcg
     2881 ggggatggcg agttcaattt cccgcgcaag ttgtgagttc ttccggcgtt cggccttctc
     2941 aacggcattc cagagttcgg cacggttcgt acaccaggca ggggctgtcg cgggagtcag
     3001 aatcacagct tcagccacac cgcttttgcg ggtgtagtcg tgagtcagtc cggtacgctc
     3061 atcaagcatc agtgaaccgg aacgataggc agcagaagca acggcgctgt agccgctgtt
     3121 tctggagatg attttcatgc tgagatgata aatcgccatt tctgccgtaa tgcctttttg
     3181 tggttttctt tcagccccgc agggcgcaca caatcgaaac ggagtggaga ttgtataagt
     3241 gcgcacttcg tgattttccg tgcattatgc cagctcagtt tttaaactac cagtgacgcg
     3301 ctaaaatgcg atttttcatc gcgcacggag ttacatcatg agcagaacac tcgaacagaa
     3361 gattgctgat gctgaggcca gattacagcg cctgaaggcg aagagcagga gtctggacac
     3421 agcgcagaag gtcattgtgg gtgcagcatt gctggcaaag gtcagaaagc cggaggaggt
     3481 gcagttgcgt gcctggttac tccagttcct gaaggcagag gtgacacgac aggctgatgt
     3541 gacgcgcata ctgccgctga ttaacgagct ggaagcgctg ccaggacagt gattgtatcg
     3601 tccgctcgcc gcagtcccag cgaccgagcg ttagttagcg agcgagtggg cgaggaagcg
     3661 gaagtgaatc cgtcgcctga ttgagcacat acgcacggtt tttcgggagg tgatatgcgc
     3721 tgaacccgca tgaatactgg atttccacag ggttggaggg ggtgtattcg gaacttgcaa
     3781 ttctgctttt ttgcaacgca ttgtttttat tggttttttg ttttcagcac tccaagttat
     3841 ccacaggctc gtcgttaagt ttttagatct tgtttttaag atcttgtttt taatacttgt
     3901 tttagcgcca aaattcctta tagaaaacaa aatgttacaa cgctataggt aatatgattt
     3961 aaggtaaagg taatatgatt taaggtgttt aggtggagga atttaaggta aaggcaatat
     4021 gatttaaggt aatattcaca gaaaaaggga tattaaaata aaaaggcatt gcctttcgtt
     4081 gagggt
//
