LOCUS       NZ_CP004878             2062 bp    DNA     circular CON 10-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. HD-1 plasmid pBMB2062,
            complete sequence.
ACCESSION   NZ_CP004878
VERSION     NZ_CP004878.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02898393
            Assembly: GCF_000717535.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. HD-1
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. HD-1
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Complete genome sequencing of Bacillus thuringiensis subsp. kurstaki
            type strain HD-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2013) College of Life Science and Technology,
            State Key Laboratory of Agricultural Microbiology, Huazhong
            Agricultural University, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000717535.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 17:51:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,021
            CDSs (total)                      :: 6,879
            Genes (coding)                    :: 6,368
            CDSs (with protein)               :: 6,368
            Genes (RNA)                       :: 142
            rRNAs                             :: 13, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 511
            CDSs (without protein)            :: 511
            Pseudo Genes (ambiguous residues) :: 0 of 511
            Pseudo Genes (frameshifted)       :: 162 of 511
            Pseudo Genes (incomplete)         :: 345 of 511
            Pseudo Genes (internal stop)      :: 150 of 511
            Pseudo Genes (multiple problems)  :: 115 of 511
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004878.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     HD-1"
                     /mol_type="genomic DNA"
                     /strain="HD-1"
                     /serovar="kurstaki"
                     /isolation_source="Soil"
                     /db_xref="taxon:1261129"
                     /plasmid="pBMB2062"
                     /geo_loc_name="USA"
                     /collection_date="1970"
     gene            25..870
                     /locus_tag="BTK_RS34300"
                     /old_locus_tag="BTK_35341"
     CDS             25..870
                     /locus_tag="BTK_RS34300"
                     /old_locus_tag="BTK_35341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_155269726.1"
                     /translation="MIKYKDVTEKQFSDVLTKISSKQIFLPNTPIRSEHGTSVRDYHRV
                     IHIGYGEGAVYIGWKHNSEKEKDSYDMKVDFNPSKFENNELQKDSYEKVFETVFHTLNA
                     VLKSNKRVVYGMDIAFDIERHMSDIVSYSKTGKQQDRHKGTVYYGNRNKDGYLKIYDKK
                     KELYNHFKRMIEEENLTRIEYSWRDSDGVVVDEIRKSPPFSIDESYTFSIFNLNNVKGA
                     LKACLICYSNGTMDMKEFPRRTKESIKKALEEMDHLAVDPILQDCWLSILENIKNYTRL
                     "
                     /name="hypothetical protein"
     gene            1058..1300
                     /locus_tag="BTK_RS34305"
                     /old_locus_tag="BTK_35346"
     CDS             1058..1300
                     /locus_tag="BTK_RS34305"
                     /old_locus_tag="BTK_35346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001293418.1"
                     /translation="MSEMVRVNTRISKKLNDWLDEYSKESGVPKSTLVHLALENYVNQK
                     VMLEQMPKMQQMLSMMFENVTQQQLNQKGNMFELK"
                     /name="hypothetical protein"
     gene            1735..1962
                     /locus_tag="BTK_RS34310"
                     /old_locus_tag="BTK_35351"
     CDS             1735..1962
                     /locus_tag="BTK_RS34310"
                     /old_locus_tag="BTK_35351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002100596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000572905.1"
                     /translation="MIESEGNNLKLTERQLNDLKRISELRVKLFGVPGESVVDPENVEF
                     LLDNAISSYLGQLEIFEVTIEIEQYNSMCG"
                     /name="hypothetical protein"
     Typing          2..870
                     /name="rep_cluster_1434"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaagttt atttggatag gctaatgatt aagtataaag atgtaacaga gaaacaattt
       61 agtgatgttt taactaaaat atcgtcaaag cagatttttt taccgaatac acctattagg
      121 tcagaacatg ggacgtctgt tagagattat catagagtta tacatattgg atatggtgaa
      181 ggtgcagttt atatagggtg gaaacataat tcggaaaagg aaaaagatag ctatgatatg
      241 aaagttgatt ttaacccttc taaatttgaa aataacgagt tgcaaaaaga tagttatgaa
      301 aaagtgtttg aaaccgtttt tcatacgtta aatgcagttt tgaagtctaa taagcgagtg
      361 gtttatggta tggatattgc ttttgatata gagcgtcata tgagtgatat tgtgtcttat
      421 agtaaaacag gaaagcaaca ggatagacat aaaggaactg tttattatgg aaatagaaat
      481 aaagatggat atttgaagat atatgataag aaaaaggagt tatataatca ttttaaaaga
      541 atgatagaag aagagaattt gactcgtatt gagtatagtt ggagagactc tgacggtgta
      601 gtggtagacg aaataaggaa gagtcctccg tttagtattg atgaatctta tacattctcg
      661 atttttaatt tgaataatgt taaaggggca ttaaaagctt gtttgatttg ttattctaat
      721 ggaactatgg atatgaaaga gttccctcgt agaactaaag agagtataaa aaaagccctt
      781 gaagaaatgg atcacttggc ggtggacccc attctacagg actgttggtt atctatatta
      841 gaaaatatta agaactatac tcgtttatga tattagcgtg tgcttctctg tgtgtcaaga
      901 gggtgtcaat atgatgctct ctttttgttt ttcttaactt gtttatatta atagcggata
      961 gagtcccact tttacattgt tctggtgtat cttagtgttg atattgtgtt taaactgatg
     1021 ttatatttat gtagtacgat atacaagagg tgattagatg agtgaaatgg ttcgtgttaa
     1081 tacacgtatc agtaaaaagt taaatgattg gttggacgag tatagcaaag aaagtggtgt
     1141 accgaaaagc actttagttc atttagcttt agagaattat gtgaatcaaa aggttatgtt
     1201 ggaacaaatg ccaaagatgc aacaaatgtt gagtatgatg tttgaaaatg taacgcagca
     1261 acaattgaat caaaaaggga atatgtttga gttgaagtaa cggttatgtt tcgaaaatgt
     1321 agtcttattg attaggagat gcacatcgat aaactaaatg cgtagttggt gtggctgaag
     1381 tttgcccgcc acctactcat ttagaatatc cgtgcatggg tccctgaaca attaggaaac
     1441 ggcttttgta atcgtatgag ttgtaattat tattcacttg ggtgaacgtt gtatgactga
     1501 aaggaaaccc atggcagtta actgtgtaaa tgggcactta tttattaagt gtattcgacg
     1561 gagccggaag tagacgactt aagggagcga catgaatgaa gtgaagtgca gacagaaggt
     1621 aaggtgacgg agtggactct caaaggacac gacggcgcgt gaactgatgg caaacgaaat
     1681 ggaatgagtg agaagagcgt acggtcggcg aggtaacgga ggtgtaggag cagattgata
     1741 gaaagtgagg gtaacaattt gaaactgaca gaaagacaat tgaatgattt gaaaagaatt
     1801 agcgaattac gtgtaaagtt gtttggagtt cctggtgaaa gtgtagttga tccagagaat
     1861 gttgagtttt tattggataa tgctattagt tcttatttag ggcaattaga aatttttgaa
     1921 gtcacgatag agattgaaca gtataattca atgtgtgggt aaattgtaga aatgtggcga
     1981 agacattttc ggacattcta atagccgaaa atcgtgtaca aaatgacatg tttaataaaa
     2041 aataaggagc gggatagatt tt
//
