LOCUS       NZ_CP004866             7921 bp    DNA     circular CON 10-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. YBT-1520 plasmid
            pBMB7921, complete sequence.
ACCESSION   NZ_CP004866
VERSION     NZ_CP004866.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02776149
            Assembly: GCF_000688795.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. YBT-1520
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. YBT-1520
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Complete genome sequencing of Bacillus thuringiensis subsp. kurstaki
            type strain YBT-1520
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2013) College of Life Science and Technology,
            State Key Laboratory of Agricultural Microbiology, Huazhong
            Agricultural University, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.0
            Genome Coverage                   :: 1300x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000688795.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 17:49:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,741
            CDSs (total)                      :: 6,598
            Genes (coding)                    :: 6,085
            CDSs (with protein)               :: 6,085
            Genes (RNA)                       :: 143
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 513
            CDSs (without protein)            :: 513
            Pseudo Genes (ambiguous residues) :: 0 of 513
            Pseudo Genes (frameshifted)       :: 286 of 513
            Pseudo Genes (incomplete)         :: 261 of 513
            Pseudo Genes (internal stop)      :: 84 of 513
            Pseudo Genes (multiple problems)  :: 91 of 513
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004866.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     YBT-1520"
                     /mol_type="genomic DNA"
                     /strain="YBT-1520"
                     /serovar="kurstaki"
                     /isolation_source="soil"
                     /db_xref="taxon:570416"
                     /plasmid="pBMB7921"
                     /geo_loc_name="China"
                     /collection_date="1990"
     gene            join(6636..7921,1..97)
                     /locus_tag="YBT1520_RS32625"
     CDS             join(6636..7921,1..97)
                     /locus_tag="YBT1520_RS32625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000648.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_040120002.1"
                     /translation="MHNVLKSSLLDVTIDKLSIVADFKEGIKKEEFIRMVASSNMPYSL
                     QANRSRNFGYEEVLKSESVDCGYIELAGELKVASVDRPKLTFHRFRLQDQIEQERLTRK
                     NGEPSLSDVEYQGLFESLADVEELIAETDEDGRLTDERKLKRQEKQVELTIEKLKGEIV
                     ALKSEIDEQELATGFKNEAMEKIHEEHVQLLLNTLKNKKNLIEVKKDELLRNAEMFLQL
                     DENGFLPGNGKKKTDRLLKNVRFEFNPKHMAYNRVVDESIRLVLGLLENVEVTGIHIAC
                     DYPVKISNLKIKDLSSKSECIFLDRDKSLGTMYVGRRGSDNHLCIYDKKQENRENDTLD
                     QYPDVEHVTRFEARLRKKKAREWIESDYNPFDSIIVGDLVNLDDSEMKLGDRIILEAIM
                     NDYHGRYFGMMDRFQRNTWRKKLNQCVPKMFDVAGDYEKKKVLLTGQLAELLENGKNKV
                     GK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            529..735
                     /locus_tag="YBT1520_RS32630"
                     /old_locus_tag="YBT1520_34196"
     CDS             529..735
                     /locus_tag="YBT1520_RS32630"
                     /old_locus_tag="YBT1520_34196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000947848.1"
                     /translation="MLSEQVIYYWNDELQYYYLAEKKNGSYKFVQPLKKLELDDISDPL
                     EREQMKKMLEIKKRMSETKGNWF"
                     /name="hypothetical protein"
     gene            complement(1440..1703)
                     /locus_tag="YBT1520_RS32635"
                     /old_locus_tag="YBT1520_34201"
     CDS             complement(1440..1703)
                     /locus_tag="YBT1520_RS32635"
                     /old_locus_tag="YBT1520_34201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874968.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPSELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(1717..2004)
                     /locus_tag="YBT1520_RS32640"
                     /old_locus_tag="YBT1520_34206"
     CDS             complement(1717..2004)
                     /locus_tag="YBT1520_RS32640"
                     /old_locus_tag="YBT1520_34206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040120000.1"
                     /translation="MAYLTAKKVKGNIYFYVSQYVGTQPYYSKHYKYINIYSIGNQKIA
                     LERIAMWLLDNNRIPKELIEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            2193..2420
                     /locus_tag="YBT1520_RS32645"
                     /old_locus_tag="YBT1520_34211"
     CDS             2193..2420
                     /locus_tag="YBT1520_RS32645"
                     /old_locus_tag="YBT1520_34211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000780193.1"
                     /translation="MKLVNNIRMIMAQKNIDNIAELIRITGVSRNSINKLWHNESVSSL
                     RLDTLIAICEKLDVKLSDLIEYIPGDIESK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3527..4732
                     /gene="mobV"
                     /locus_tag="YBT1520_RS32655"
                     /old_locus_tag="YBT1520_34221"
     CDS             3527..4732
                     /gene="mobV"
                     /locus_tag="YBT1520_RS32655"
                     /old_locus_tag="YBT1520_34221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_040120001.1"
                     /translation="MSRFMVCHMQKFKMTDVKGLQIHNQREKESHSNPDIIQERTEQNY
                     DLIHGKENVDYKKLIQDRIQDGVVSDRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEKLEQEKKEIDSRLHDLKL
                     SLDKAKSVDEIPVKEKGGFIRSKTVEIALEDFESIKVLAKSSETLREENKHLKNEKVKD
                     EYEKDNLYKEQRFLERKVTDLKRENEGLKGENDFLKKTLERVKDLYKEKLPELAGMIGY
                     VKASILDKMNRKFLKRHFAGDDEVSGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            4764..5045
                     /locus_tag="YBT1520_RS32660"
                     /old_locus_tag="YBT1520_34226"
     CDS             4764..5045
                     /locus_tag="YBT1520_RS32660"
                     /old_locus_tag="YBT1520_34226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002026684.1"
                     /translation="MDYFHGYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNEQIMGLGEGLSAISERLRDMSEGIALISMDDKE"
                     /name="hypothetical protein"
     gene            5310..5519
                     /locus_tag="YBT1520_RS32665"
                     /old_locus_tag="YBT1520_34231"
     CDS             5310..5519
                     /locus_tag="YBT1520_RS32665"
                     /old_locus_tag="YBT1520_34231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001174425.1"
                     /translation="MQKVIVVNGYWHNSNGKWKDTTEELNGLLAEGWKVVNIAPMGAYG
                     FGYDAMGNTANSDHGFASIVVIEK"
                     /name="hypothetical protein"
     gene            5782..6132
                     /locus_tag="YBT1520_RS32670"
                     /old_locus_tag="YBT1520_34236"
     CDS             5782..6132
                     /locus_tag="YBT1520_RS32670"
                     /old_locus_tag="YBT1520_34236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000562819.1"
                     /translation="MICDNCMNEVEKEFCQTCHYFGYVEVRYKCSNCSYEGKTIENLDD
                     KVNELFKKGRKLIIEMDTDFKPYLEILVKRGITNPKEVGILFGMYLESKKEDHEGYKTI
                     SKSGFEKFLTTI"
                     /name="hypothetical protein"
     gene            6269..6475
                     /locus_tag="YBT1520_RS32675"
                     /old_locus_tag="YBT1520_34241"
     CDS             6269..6475
                     /locus_tag="YBT1520_RS32675"
                     /old_locus_tag="YBT1520_34241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001029683.1"
                     /translation="MAKANYVTVGRKPKNGKTPKVQEAFRLPKETVDRINELVNSGRFS
                     KKIDVAEKAFEFYFAHLDKEERK"
                     /name="hypothetical protein"
     Typing          6637..7921
                     /name="rep_cluster_268"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3528..4729
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.524"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttgatgtt gctggggatt atgaaaagaa aaaagttcta ctaactggac aattagcaga
       61 acttctagaa aatggtaaaa ataaggttgg aaaataaaaa aaatggttaa tcctttcgag
      121 tgttggcgca cttggaagga tttttttatg atttagaact taattttatt tttaagaata
      181 actacgacta ttattgcatg tataccgtat attattcatt ttaatgactt ttatattgat
      241 tgtcatcaaa gaaaaatacc ccaaaaaggg tgcggttttt tatatatata aaacatataa
      301 ccctaccgtt cataaattat atatattttt atgaacgttt gcaaatgatt ttagggactt
      361 tttgagacac ttgtttttgg ctttatgatg cgtttatcag ttatttggga ctactttttg
      421 atacggttac ttttggaact gtctcttttg tattgcgtta attttagggc tttattgatg
      481 ctatgatggt gtaatgggct aatattaagg tgattactgg gggcttttat gttgagtgaa
      541 caagtcatat attattggaa tgatgaatta cagtattatt atttagctga aaaaaagaat
      601 ggcagctata aatttgtaca accgttaaaa aaattagaat tagatgatat tagtgatccg
      661 ttagaaagag aacaaatgaa aaaaatgcta gagataaaaa aacggatgtc agaaacaaaa
      721 ggtaattggt tttaaaaaaa agaaagacaa aatgtctttc ttttttttaa aactttaatt
      781 tcgtgaattt gtgaattctt ttttaaattt ataagctata tagtttatat cctaaaactt
      841 cccgttacta ccgtacagga tggaaaacta aagtgtgcgc atgaattttt ttagttatat
      901 atataacgat aaaataccgt acgttttttg aaggtttatt ttttggggga gattttgatg
      961 tggaatttga ttttgaactt tgttgaattt gaaatctcga tttttcgatt tttggaattt
     1021 ttgttttctg ttttctttgt aattccgtat tccgaaatta cggagtttat atagtatctc
     1081 tatgatgaac tgaaaacgaa aaaaactttg ctcgcatttt catttcatag atatatatat
     1141 cattaaaaag tcgcaccgtt ttaatcgagt tgtgtgatga aaaaaagatg tttagattat
     1201 gtttgaattt gttgttttgt gaatttttta tttcgtgatt tctgtatatt tttaatcctt
     1261 tttactctta aagtctgttt gtttttgttc gcaaatgaaa aattcacaaa gtcacgcata
     1321 acttttatta aatatataaa tatattatta aaatatctaa cccttttgtg atgaaaaaaa
     1381 tataaatagt taaaggaaaa aagactgata aaaatatcag tcttttttct tgtttttagt
     1441 tatagagagt atctttttat ggtattttct actttttctc tccagttttc tatatcgtca
     1501 attgatattc cgatctttat taattcgctt ggaatgaaac tgttatctaa aatccataaa
     1561 gtgagtcttt ctaatgtgat tctttcgttt ccaatcgaat aaatacgttc actttgtttg
     1621 caggtatatg gttttttacc aacatatttt tctacataaa agtatctttt tccatttgct
     1681 ttgtaatttg ttagaaatgc catagtaatg ctccttttag gtatttttat ggttagttaa
     1741 agagtagttt tgtaatgttt tttcaacttt ttcataccaa tatttcacat cgtttatgga
     1801 tactccgatt tctattagtt cttttggtat gcggttgtta tctaataacc acatagcgat
     1861 tctctctaaa gctatttttt gattgccgat tgagtaaata tttatatatt tatagtgttt
     1921 tgagtaatat ggttgtgttc caacgtattg agatacataa aagtaaatgt ttccttttac
     1981 tttttttgct gttagatatg ccaaatcgtt cttcctttca tgtttgttat tcttttgttt
     2041 ttgtctgtgg ttatatatta accttataaa gttaaaatat caatacatat aaataaatat
     2101 aaaatttaaa aggtttattt ttatttataa attgcataaa attatcttta taagtaaaat
     2161 agaatgtatg ttgaatataa ggagtaagag gaatgaagtt agtaaataac attcgtatga
     2221 ttatggccca aaaaaatata gataatattg cggagttaat aaggataact ggtgtgagtc
     2281 gtaattctat taataagctt tggcataatg aaagtgtgtc gtctttaaga ttagatactt
     2341 taatagcgat ctgtgagaag ttagatgtta aattatcgga cttaattgaa tatattccag
     2401 gtgacattga gtcaaaataa tttaggggat tttatgtact tggatagttt ttgtatgcta
     2461 aaggtatagt tggctaatgg tatttggtgg tgctataagg cgcacttcaa cttttttgtt
     2521 aacgcttgag tgtggtttgg attagggata tgaaattgta gacttgctag gtgcgtcaac
     2581 accttggaag atgagaggta ctggcatact tcgtgatttt gttgaacttt atttataatc
     2641 atagtagtgc ggttaaatga aatggtggta ttgattagta ttgaagaaag agtcctattt
     2701 tagggctctt ttttattttg ccttttaaag ggcttaaatc gtttttaaaa cattgtgagg
     2761 ctcttaggat ttcttaagaa ctagaagttc ctagaatgca ttctgaacgt tctgaggtgt
     2821 atttctctat ggattctgtc ttggaatggg tctaggaata gctgtaggaa tagctgtagt
     2881 tttattctat ggttgttccg gtagtggttc tcatgaagaa tatgattgta tcaatagatt
     2941 tgatgatgtt tgaaaagtag agaatagttg aagctcttcc tgtaaaacaa aaaaataatt
     3001 atgtttgtag aggaagtagt catttcactc caagaaaatc cctgtagatc ggtggggcgg
     3061 tgaaaaaaaa tagggaagat tagcgagggt attttgcagg gcagcggaag acgttatgtt
     3121 gtcgtgatga atataaaaag agacttttta atacatggat gttagctgaa aaacgtttga
     3181 ggtagttgta tgatgtttgt ccaacggtaa gcggaacgaa gtgagcatta aggagtggac
     3241 gaacccactg tgcccagaac cctcttggaa ctccaaaacg ttagccgttt tggggatgcc
     3301 taccggcgag ggagtccctc aaggattgag gggttgggga gttcgattga aaggggtcaa
     3361 ggggaagagt ttcccttgtg ggtttgggca aagcccatga ttcttttgcc atgcttgtca
     3421 tggctcggcg gtcgccgaaa tgtcccgtag ggcacacgtg tttacgcagt aaactgtagt
     3481 gtgctacagt ttacatggaa ctaccaccga aaaaggagcg aagcccatgt caagattcat
     3541 ggtttgtcat atgcaaaaat tcaagatgac agatgtgaaa ggattacaaa tacataatca
     3601 acgtgaaaaa gaaagtcatt caaatccaga tattattcaa gaacgaacgg aacagaatta
     3661 tgatttgatt catggtaaag agaatgtgga ttataaaaaa ctaattcaag atagaattca
     3721 agacggggtt gtatctgata gagccattag gaaagatgct gttgtttgtt gttcgtttat
     3781 gatttcggct agtcctgatt atatgaattc tttatctcaa gcggaacaga gacgattctt
     3841 tgaagagtct gtttgttttt taaaggcaag gtatggagaa gagaactttg tatatgcttc
     3901 agtacatgtt gatgaaaaga cacctcatat gcatgtgggc atggttccag ttaatgaaaa
     3961 acaaaaacta tcagcgtatt cattttttaa aaacaaatca gagcttcatg atctacaaga
     4021 taaaatctat gaacatgtga aagaaaaggg atttgatata gagcgtggtg tttctagcga
     4081 caggaaacat ttatcgacgc agcggtttaa agcagtgact ttacaacaag aaatagagaa
     4141 gttagaacag gaaaagaaag agatagatag tcgtcttcat gatttgaaat tatctttaga
     4201 taaagctaaa agtgtagatg agattcctgt taaagagaaa ggtgggttta tacgctctaa
     4261 aacggtcgag attgcattag aagactttga aagtataaaa gtattagcta agtcttccga
     4321 aacgcttaga gaggaaaata aacaccttaa aaacgaaaag gttaaggatg aatatgaaaa
     4381 agataattta tataaagaac aacgtttttt agagaggaaa gtaactgatc taaaaagaga
     4441 gaatgaagga ttaaagggcg aaaatgattt tcttaagaaa acgttggagc gagtgaaaga
     4501 tttgtataag gagaagctcc ctgaacttgc aggaatgatt ggttatgtta aagcgagtat
     4561 actggataag atgaatcgca agtttttaaa acgacatttt gctggcgatg acgaagtaag
     4621 tggtgctcaa aagttcttga atcacaaaca agaacatgaa gaacaacaaa agagattaaa
     4681 acaagtgcga agaagccaac agaaaaatcg ggaccaggga ttggagagat agtgtgggga
     4741 cattatttaa tcagcaacca agaatggatt attttcatgg ttatggagtt aagttcatag
     4801 gtactgtgaa ggaattggct aagaaaaata atttaacgat tgaagagacg tgtagaattt
     4861 tagaattgac tatgaaaatt gatgattatg atcgtaagga tgagcagtta gcaggtattg
     4921 gagaacttat taaggaaatg aatgaacaaa ttatggggct tggtgaaggt ttgagtgcta
     4981 taagtgaacg attgcgtgat atgagtgaag gtattgcgtt aatatctatg gatgataaag
     5041 aataatggta tgttaggagg ctctgtgtga gtctcttttt ttctttagac gtgtagtgtt
     5101 agcggaactt gtttggcata atttaacgag gtttgtagag ctttgcataa tgtcctcgca
     5161 aattgttgat gtgaatatat ggggatatta tataaggctc ttgggaatgg ctcttgtttt
     5221 ttaaaatatt tatttagata tatatattaa aattgtattt gatatataga aatttatata
     5281 ttgataatca aaacgaggga gaggtatata tgcaaaaggt aattgtagtt aatggttatt
     5341 ggcataatag taatggtaaa tggaaggaca ctacagaaga attgaatgga ttgttagcag
     5401 aaggatggaa agttgttaat atagcaccta tgggggctta tgggtttggt tatgacgcaa
     5461 tggggaatac ggctaattct gatcatggtt ttgcctcgat agttgttatt gagaagtaat
     5521 agaaaagacg tgggatttca ccacgtcttt ttttattttg aaagtaagat taaggaataa
     5581 ccccccctat gtaatacggg ttgtgtgacc tcaaaaactt agggggtaat tctattgaaa
     5641 atacagggat attaaggctt taaggtgtgt tttcttttct tttaaaaatg gaattttaaa
     5701 agaatgaaca aaaaatacag taaaatatac aaaaggtaga cggtaaaaaa atagatatta
     5761 tgtttctgta gggggagaaa aatgatttgt gataattgca tgaatgaagt agaaaaggaa
     5821 ttttgtcaaa catgtcatta ttttggttat gttgaggttc gatataaatg ttcgaattgt
     5881 agttatgaag gcaaaacgat tgagaactta gatgacaaag taaatgaact gtttaagaag
     5941 gggagaaaac ttattattga aatggataca gattttaagc cttacttaga aattctagtg
     6001 aaacgtggta tcacgaatcc taaagaggtt ggaattttgt ttggtatgta tttagaaagt
     6061 aaaaaggaag atcatgaggg atataaaact atttctaagt ctggatttga aaaatttcta
     6121 acaacgattt gagttttagt aggtgagcaa tttttgataa tttgtagact ttgaagttta
     6181 tattgaaatg aattttaaaa ttaaacttgt taaaatggta tgcaaaatga tatcatgata
     6241 ttgttggttt tatcgagggg gtgaaaagat ggctaaggcg aattatgtga ctgttggaag
     6301 gaagcctaaa aacggaaaaa ctccaaaagt acaagaagct ttcagattgc cgaaagaaac
     6361 ggttgataga attaatgaat tagtgaatag tgggcgtttt tctaaaaaga tagatgttgc
     6421 agaaaaagcg tttgaatttt attttgctca tttagacaaa gaagaaagaa agtgatttgt
     6481 ttttagtgtt ttttggtggg gaagaaatag tccgatgaaa aaaagttacc tgttattcta
     6541 ataagcaggt aaaggttgag aatttctcac acttcttaaa aaaacatctc ggacaagcgc
     6601 acaaaaatat ctgttttggg ggttaaggat ttgttatgca taacgtactg aaatcaagtt
     6661 tgttagatgt aacgattgat aaattaagta ttgttgcaga ttttaaggaa ggcattaaaa
     6721 aagaagagtt tatacggatg gtggcgagtt caaatatgcc gtattcgtta caggcgaatc
     6781 gttcgaggaa ttttggttat gaagaagttt taaaaagtga atctgttgat tgtggttaca
     6841 ttgagttggc aggagaattg aaagttgcat ctgttgatcg tccgaagtta acttttcata
     6901 gatttagatt gcaagatcaa attgagcaag aaaggttaac gcggaaaaat ggtgaaccat
     6961 cactttctga tgtggaatat caaggtttgt ttgaaagttt agctgatgta gaagaattga
     7021 tagctgaaac tgatgaagat ggtcgtttga ctgatgagag aaaattaaaa cggcaagaaa
     7081 aacaagtgga attgactatt gagaagttga aaggcgaaat agttgcattg aaatctgaaa
     7141 tagatgagca agaacttgca acgggtttta aaaatgaagc tatggaaaaa attcatgagg
     7201 aacatgttca gttattactt aatactttaa agaacaaaaa gaacttgatt gaagttaaaa
     7261 aggatgagtt gttaaggaat gctgaaatgt ttttacagtt agatgaaaat ggttttcttc
     7321 cgggtaatgg aaaaaagaaa actgatcgtt tgttgaaaaa tgttaggttt gagtttaacc
     7381 ctaagcatat ggcatataat cgtgtggttg atgagtcgat acgattagtt ttggggttgt
     7441 tagaaaatgt tgaagttaca ggtatacata tagcttgtga ttatcccgtt aagatttcaa
     7501 atttgaaaat taaagattta tcaagtaaat cagagtgtat ttttctagat agggataaat
     7561 ctttaggaac gatgtatgtt ggaagacgcg ggtcagataa tcatctttgt atttatgata
     7621 agaagcaaga gaatagagag aatgatactt tggatcagta tccagatgtg gaacatgtaa
     7681 ctcgttttga agctcgttta cgtaagaaaa aggcgagaga atggatagag tcagattata
     7741 atccgtttga ttcgataatc gttggggatt tagtgaattt agatgatagt gaaatgaaac
     7801 ttggtgatag gattatttta gaagctatta tgaatgatta tcatgggcgt tattttggga
     7861 tgatggatag atttcaaaga aatacatggc gtaagaaatt gaatcaatgt gttccaaaaa
     7921 t
//
