LOCUS       NZ_CP004864             8513 bp    DNA     circular CON 10-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. YBT-1520 plasmid
            pBMB8513, complete sequence.
ACCESSION   NZ_CP004864
VERSION     NZ_CP004864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02776149
            Assembly: GCF_000688795.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. YBT-1520
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. YBT-1520
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Complete genome sequencing of Bacillus thuringiensis subsp. kurstaki
            type strain YBT-1520
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2013) College of Life Science and Technology,
            State Key Laboratory of Agricultural Microbiology, Huazhong
            Agricultural University, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.0
            Genome Coverage                   :: 1300x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000688795.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 17:49:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,741
            CDSs (total)                      :: 6,598
            Genes (coding)                    :: 6,085
            CDSs (with protein)               :: 6,085
            Genes (RNA)                       :: 143
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 513
            CDSs (without protein)            :: 513
            Pseudo Genes (ambiguous residues) :: 0 of 513
            Pseudo Genes (frameshifted)       :: 286 of 513
            Pseudo Genes (incomplete)         :: 261 of 513
            Pseudo Genes (internal stop)      :: 84 of 513
            Pseudo Genes (multiple problems)  :: 91 of 513
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     YBT-1520"
                     /mol_type="genomic DNA"
                     /strain="YBT-1520"
                     /serovar="kurstaki"
                     /isolation_source="soil"
                     /db_xref="taxon:570416"
                     /plasmid="pBMB8513"
                     /geo_loc_name="China"
                     /collection_date="1990"
     gene            join(8400..8513,1..468)
                     /locus_tag="YBT1520_RS32535"
                     /old_locus_tag="YBT1520_34086"
     CDS             join(8400..8513,1..468)
                     /locus_tag="YBT1520_RS32535"
                     /old_locus_tag="YBT1520_34086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000723966.1"
                     /translation="MKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAATTN
                     NNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYGNDV
                     GLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWKEAA
                     KIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     gene            461..658
                     /locus_tag="YBT1520_RS32540"
                     /old_locus_tag="YBT1520_34091"
     CDS             461..658
                     /locus_tag="YBT1520_RS32540"
                     /old_locus_tag="YBT1520_34091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     gene            2031..3236
                     /gene="mobV"
                     /locus_tag="YBT1520_RS32550"
                     /old_locus_tag="YBT1520_34106"
     CDS             2031..3236
                     /gene="mobV"
                     /locus_tag="YBT1520_RS32550"
                     /old_locus_tag="YBT1520_34106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033305.1"
                     /translation="MSKFVICHMQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNY
                     DLIHDKEKVDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRF
                     FEESLRFLKGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKM
                     SLDQAKSVDEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVI
                     TREKDGLYREKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGY
                     VKGSILDRMNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            3706..4752
                     /locus_tag="YBT1520_RS32555"
                     /old_locus_tag="YBT1520_34111"
     CDS             3706..4752
                     /locus_tag="YBT1520_RS32555"
                     /old_locus_tag="YBT1520_34111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            4756..4953
                     /locus_tag="YBT1520_RS32560"
                     /old_locus_tag="YBT1520_34116"
     CDS             4756..4953
                     /locus_tag="YBT1520_RS32560"
                     /old_locus_tag="YBT1520_34116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     gene            complement(5822..6085)
                     /locus_tag="YBT1520_RS32565"
                     /old_locus_tag="YBT1520_34121"
     CDS             complement(5822..6085)
                     /locus_tag="YBT1520_RS32565"
                     /old_locus_tag="YBT1520_34121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            6359..6586
                     /locus_tag="YBT1520_RS32570"
                     /old_locus_tag="YBT1520_34126"
     CDS             6359..6586
                     /locus_tag="YBT1520_RS32570"
                     /old_locus_tag="YBT1520_34126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            7058..8137
                     /locus_tag="YBT1520_RS32575"
                     /old_locus_tag="YBT1520_34131"
     CDS             7058..8137
                     /locus_tag="YBT1520_RS32575"
                     /old_locus_tag="YBT1520_34131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102658.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCNQEYKI
                     HFLFLQALNNKLSTEQLEKVMLDAIPYFEKQNLWKYIQDYTEELAIRFYEEKNKDKSNE
                     YFYKSYKAKRNLLERESLK"
                     /name="RapH N-terminal domain-containing protein"
     gene            8134..8280
                     /locus_tag="YBT1520_RS36510"
                     /old_locus_tag="YBT1520_34136"
     CDS             8134..8280
                     /locus_tag="YBT1520_RS36510"
                     /old_locus_tag="YBT1520_34136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000976019.1"
                     /translation="MMKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHG
                     KDI"
                     /name="hypothetical protein"
     Typing          3682..4734
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          2032..3233
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttttgcgg caactacaaa taacaacaat ataatacaaa cgaaacagtc tctcgtttct
       61 ggtcaagatg aaaagagcat taatgaatta gcagaagcgt ttcaatttat gcatgaagaa
      121 gccgctatat ataataatca aggtgaaatt gttgattttg attttaataa aatagaagcg
      181 aaatatggga atgatgttgg tttagatgca cttaaacaac aaattaaaaa agataaaagt
      241 gatattgctg gtttatcttt tacaagttgc atgaaagatg ctttatatga cttttttgga
      301 gtaaatgctg tacaagccgc atttaatggt ggattggcat attatcttga gaaaaaatta
      361 tggaaagaag cggctaaaat aggcgttaaa ttctttatag gaagtagcgt agcaggttta
      421 gcggctactt tagcttatta tggaggtaag tgtgcaattt atggataaaa gttcaatttt
      481 aaaacaaatg tattttattt tatttccggt aacacttttt ctatctctaa atatttatca
      541 atggattgtt aaaagtgatt actcatttac aggatatata atccaaaatt taattattgt
      601 agcattagcg ttagtagcag ctatcatagc ttatttaatt actaaaaaaa atatttaaca
      661 cataatgtta gtctaaataa cagaaaattc taacccttat tcttattttg tattatactg
      721 gttatgtaca ggtaaaatgg tttacaagtt aatttttcct cgtttcaaaa aaagtccatt
      781 cttttcaaaa gaatggactt ttttcttgtt atatttgctg taactattaa aagggcatca
      841 aaacgatcta caaaagcctc aagacgaccc acaaaccatc tttaaacggt ctcaaaacga
      901 actacaaccc gaaaaaacgc atcccagttt ttttaggttt aattttaagc gcataccaag
      961 cgcatatagg cgcatacggg acacatacaa agtgccgaaa aacgcatata ggtcgcatac
     1021 aaacggtaga aatacatccc taaaaataga gtgatagaaa cccaataggc acagcctatt
     1081 tcaccacttt ttttgcaaag tgggattcgg tttccttatg ctcttttttt atttttgctt
     1141 tttaagggtc ttataatcgt ttttaagtat tttaggatgt tgggtttctt aagggaacta
     1201 gaagttccta gaatggattc tgagcgttct gaggtgtatt tttctatgga ttcgatattg
     1261 gaattgctct aggaatagtt gtcgttttat tctgtggctg ttccgacagt ggttctcata
     1321 aagaatatga ttgtatcaat agatttgatg atgtttgaaa aatagagaat agttgaagct
     1381 cttcctgtaa aacaaaaaaa attatgtttg tagaggaagt agtcatttca ctacaagaaa
     1441 attcccatag atcggtggcg agttgaaaaa tgttgaaggt tggcgaggac attttacgag
     1501 gacgcgggaa tggctttgtc attgttgggg ttaatagttt ttaagaaaag ttggttagta
     1561 gctgaaaaaa atagggaaga ttggcgagga tgtattgcat ggcagcggga catgttatgt
     1621 agtcgtggtg aatataaaaa gagacttttt aatacatgga tgtcaactgt aaaaagttag
     1681 aggtagttgt atgatgtttg tccaacggta agcggaacga agtgagcatt aaggagtgga
     1741 cgaacccact gtgcccagaa ccctcttgga actccaaaac gttagccgtt ttggggatgc
     1801 caaccggcga gggagtccct caaggattga ggggttgggg agttcgattg aaaggggtca
     1861 aggggaagag tttcccttgt gggtttgggc aaagcccatg attttttttg ccatgcttgt
     1921 tatggctcgg cggtagccga aatgtcccga agggcacacg tgtttacgca gtaaactgta
     1981 gtgtgctact gtttacatgg aactaccccc gaaaaaaagg agcgaggccc atgtcgaagt
     2041 tcgtgatttg ccatatgcaa aaatttaaga tgagagatgt gaaaggatta caaatacata
     2101 atcaacgtga aaaagaaagt cattccaatt cagatattat tcaagcacga acggaacaga
     2161 attatgatct gattcatgac aaagaaaaag tggattataa gaaattaatt caaaatcgaa
     2221 ttgataatgg tgttgtatct aatcgtgcaa ttcggaaaga cgctgttgtt tgttgttcgt
     2281 tcatgatttc ggctagtccg gattatatga attctttatc tcaagtggaa cagagacgat
     2341 tctttgaaga gtctcttcgt tttttgaagg ggagatatgg agaagagaac tttgtgtatg
     2401 cttccgtaca tgttgatgaa aaaacacctc atatgcatgt gggtatggtt ccagttaatg
     2461 aaaaacaaaa actatcagcg tattcatttt ttaaaaacaa atcagagttt catgatttac
     2521 aagataaaat ctatgaacat gtgaaagaaa agggatttga tatagagcgt ggtgtttcta
     2581 gtgatagaaa acatttatcg acgcagcggt ttaaagcggt gactttacaa caagaaatag
     2641 aagaactaga gcaagaaaag aagggaatag atagtcgtct tcatgattta aaaatgtctt
     2701 tagatcaagc aaaaagtgtg gatgagatcc cagttaaaga aaaaggtggg tttatacgct
     2761 ctaaaacggt cgagattacc tcggaagact tcgaaagtat aaaagtatta gctaggtcgt
     2821 cggaagccct tagagaagat gttagacgcc ttaaaaacga atcaggtgta attacacgtg
     2881 aaaaagatgg gctgtataga gagaaatgta ttttagaggg gcaaataaca gaattgaaaa
     2941 gagagaatcg aggattgaag gaagagaatg atttctttaa gaaaacgttg gaacgagtga
     3001 aagagttgta taaagaaaag cttcctgaat tggctggggt gattggttac gttaagggga
     3061 gtatattgga caggatgaat tgcaaatttt taaaacgata ttttactagt gatgatgaag
     3121 tgagaggcgc tcaaaagttt ttgaatcata aacaagagca tgaagaacaa caaaagagat
     3181 taaaacaagt gcgaagtagc caacagaaaa atcgggacca gggattggag agatagtgtg
     3241 gttctgtttg agttttttgt tttttagatt tatagcgtta gcggaatttg ttttttataa
     3301 cttgcttaag atgagtatgt ttctcagatg ttttatccgt taaaaaaaat ggttttgaac
     3361 gaaaaaaaat gcccctgtcc acggagtgga tttaggggca tttttttatg atttttcgtg
     3421 ttcgaaaaat cgagtttttt ctttctttaa tacataggta actatttcgg caacgaaatt
     3481 gatgcctatg tattctttta gcccttatgt atcaagggtt tgtaaaaaaa aagacttgct
     3541 ttttcaggcg gatagattta tcatttttag tgtcgaatta aaaatcataa ataccacgag
     3601 aggagcaagt ccaaaaaaag gattcgtaca ccctttttta tatcgaatat gaaattcaga
     3661 aattagaaat tctgatgttt ttcagatagg actaatatat caaatatggc aaagaaaaat
     3721 caagatgaaa tcactggtaa attacaacct aaaaagaaac agaatatcaa gatttacgaa
     3781 tttataaaga agaaaatgag tgagagcggg aaagaattgt ttaaatcgtg cagtatcttt
     3841 aatgaatttt tggctacaaa ggataaaaag aagaagaaac gagtaaaggg gaatgattgt
     3901 aaaaatcgat tttgtccgat ttgtgcttgg aggaaagcag ggaaggacgc ggtgaaaatc
     3961 gcaacgatga tggaagcaat taagattgag gaaaagaaag agtttctttt tttaacgttg
     4021 acgactccaa atattaaagc tgatatggtg aaaagtgaga ttgatagatt taataaagct
     4081 tttaaaaagt tatttgatag acgtaatatt caacgttcta ttaagggtta tattagaaag
     4141 ctagaaatga cgtatgataa agaacgattc atcactgaag aaatgtataa caacaaaaag
     4201 agaaaagcat attatgattc tcgtggattg aaagtagacg atcataaccc gaattatgat
     4261 acatataatc ctcattttca tgtattgctt tgtgttgata aaaattattt taaacgtaaa
     4321 gagctttata ttaagcaaga agaatggtta gaaatgtggc gagaagtaac agatatgcca
     4381 gagattacgc aagtacatat tcaaaaagta gagttaatac gagaaggaaa tgcagttgcg
     4441 gaggttgcga agtattcagc gaaagactat gaaatgtcgg ttagtcaaga agtgtttgac
     4501 gtgttctatt tagcattaaa aggtcgtcaa ttaattgttt acggtggatt attaaaagat
     4561 tatgcgaaaa aatatgaaga tggcgaattg gacaagtata agagtacaga caagaatgaa
     4621 tattattata gattaatcgc aatgtggaat aaggatttaa tgaaatttga acaagagtat
     4681 caagagttat ctgagagtga gaagcaagag tataacggtc atttaattaa tgaagtagat
     4741 gtggaggcgt agtgtatgaa agtagcggga gatttatatt attattgtct tggttgtaaa
     4801 aagtttcatg aatatgaaaa aattgatcat aaaggtgtaa atagaaaatt atgtttttat
     4861 tgtttcaaga ttcaatctaa aaaaacgaaa attattggtg atgctgaagg tagaatgcaa
     4921 atttgtgaga cttgtcataa agaattgttt tagttggttg atttgtgaaa aaagcaaagt
     4981 caaatgttag aaatttttaa taagggaact gactgttata tcaagtcagt tcccttattt
     5041 tattgagtct atttttgagt gcagcaagta gcattcaaaa gtttttttat gatttaacaa
     5101 aaaggagttt ttcttaaatt taaatttcgt gaatttgtaa atttattttt aaagtttatt
     5161 tcgagatata ttttgttcta ctaattttgt tttactaacg tacgaggtga aaacttaaag
     5221 tgtgcgcatg aattttttta gtaatatata ttacgataaa ataccgcacg ttttttgatg
     5281 atttttttga aaatatagtt ttgagaaatt ctaaattttc ggggctgatt ttggattttt
     5341 tatgtgggat ttgattttgg aatttgttaa attggaaatc tcgatttttc gattttcgga
     5401 atttttgttt tctcttttct ttgttattct gtatttcgaa attaaggagt ttatataata
     5461 ccttcatgac gaactgaaaa cgaaaaaact ttgctcgcat atttaattca tagatatttc
     5521 atatcgttaa aaagttgcac cgtttcaaac gagttgtgtg ataaaaagat gtttagatta
     5581 tgtttgaaat tgttgtttct gtgaattttt tatttcgcga tttctgtata tttttaatcc
     5641 tttttactct taaaatcttt ttgcttttgt gcgcaaatga aaaaatcaaa aagtcacgcg
     5701 taacttttat taaatatata aatatattat taaaatacct aaccctttta tgatgaaaaa
     5761 atataaatag ttaaagaaaa aagactgata aaaaatatca gtcttttttc tttagtttta
     5821 tttataaaga gtatctcttt atggtgtttt ctactttttc tctccagttc tctatatcgt
     5881 taattgatat tccgatcttt attaattcgt ttggaatgaa actgttatct aaaatccata
     5941 aagtgagcct ttctaatgtg attctttcgt ttccaattga ataaatacgt tcactttgtt
     6001 tgcaagtata tggtttttta ccaacatatt tttctacata aaagtatctt tttccgtttg
     6061 ctttgtaatt tgttaggaat gccaaaatga taatctcctc ttagtttttt tagagttaaa
     6121 aatacatttt aaaagattaa attttaagtt atatatttaa ttttaactct aaggggttaa
     6181 aatttcaatt aaaacaaata aaattaaata ataaaacaat atattttatt tttaaggtta
     6241 atattaaatg tgaatatata aaaaataacc ttaatattat ttaatttatg ttttatttgt
     6301 ataaaagtat ccttgtaagt aaaatggtat gtacattgaa tgaaaaggag tatgaaaaat
     6361 gaaggtagta agtaatattc gtatgattat ggcgaaaaag aatatagata atatttcgga
     6421 tttggtgaga ataacgggag taagtcgtaa ttctattaat aagttgtggc acaatgagaa
     6481 tgtgtcttct ttaaaattag atacattgat aacgatctgt gagaagttgg atgttgagtt
     6541 attggattta attgaatata ttcgagatga tagtgagaca aaataattta ggggctattt
     6601 tagctttaga tagcttgtgt atgatgaaag catatttttg ctaatcgaca atgagttata
     6661 ttcacatatg ttttttttgt agggctatat gtgtatttaa agttttgatt tctatttgtt
     6721 attgtatgaa tatggtttga atgtggtaaa tgaaattata gtcttgctag gtgcgtcaac
     6781 accttggaag atgagcggta ctggcatact tcgtgatttc attgatagta gtgcagtaaa
     6841 gtgatggaat tgatgagtat tgaaaaagag ttcctgtttg ggggctcttt ttttatttgt
     6901 tactaagtat ttttaaatag tgaaggtgct acactattta aaaatacaga atattaaaaa
     6961 ctttatatat caactgttga aaagttagtc ttatacaata gcatgataac atgatagaaa
     7021 ataaaatata gataattttg gaaaaggaga agattctatg aatgttcaac taggaaacga
     7081 acaaattaca aaattattaa atgattggta tcaagaaatt cgagcgcaac acattattaa
     7141 agcaaaacaa ttaaaacaaa agattgaaaa agaaattaac aacatagaag aagaccaaaa
     7201 tttactttta tattattcac ttattgattt gagatacaag attcttacaa acgactatgc
     7261 agatagagaa caatctttag aaaaaatcga acaattaaaa gaacacacaa ataatttttt
     7321 agattactat tatcatttct ttaaaggcat gcatgcaatg aaaacaggaa attatagtga
     7381 aactcaaaaa tattatgata tagcagaaaa attactagaa gaaattcctg atgaagtaga
     7441 aaaagctgaa tttaattatg ctgttgcaac tttctattat catactcatc aagccttatt
     7501 agcaactcaa tatgctaaca aagccaaaag tttttttagt gggaaactag gatacgagat
     7561 taaaaccgga gcatgtgaaa acacacttgg tatggcttgt attacattaa gggaattttc
     7621 tgttgcagaa gaatacttgc tttccgcaat taataaattt gaaaaacaca gtgaaaacaa
     7681 attagcttta atcgtacgtt ataacttagg tcttttatat gcagatcaaa atctttctga
     7741 acttgcaatc cgttatcttc ttgaatcctt tgaaaatcaa gaagacgatt acaaaacaat
     7801 gtttttatta gcacaagaat attacaagtt aaaacaaaca aatacagtaa acacatatat
     7861 tgaaaaaggg ctaaatgttt gtaaccaaga atataaaatt catttcttat ttttacaagc
     7921 attaaacaat aaattatcta ctgaacaatt agaaaaagtc atgttagacg cgattccata
     7981 ttttgaaaaa caaaatctat ggaaatacat acaagattat actgaagaat tggctataag
     8041 attttatgaa gaaaagaata aggataaatc taatgaatat ttttataaaa gttacaaagc
     8101 taaaagaaac ttattagaaa gggagtcatt gaaatgatga aaaaatttag tctgatacta
     8161 ataggggtag catgtacaac aggtattttc ttttcccaat tcaataattc cattcaaaca
     8221 catgatgcta aagaaaaaaa tgatattatt caacaatatg cacatggaaa agatatttaa
     8281 aagcaaattc caacaattag caatcgacct aaatattagg attattaaca aaatattgaa
     8341 ttatatgtaa attaagttta ttatctattt tgaataataa acttaagggg atgaaattta
     8401 tgaaaaaaat tagtattgag agaagtaatt ggagaaaaac atataaaaaa atgattccag
     8461 tattgttagt cggaggaata ttggcaggaa atgtagcatt tgtgacaaat aat
//
