LOCUS       NZ_CP004863            11769 bp    DNA     circular CON 10-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. YBT-1520 plasmid
            pBMB11, complete sequence.
ACCESSION   NZ_CP004863
VERSION     NZ_CP004863.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02776149
            Assembly: GCF_000688795.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. YBT-1520
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. YBT-1520
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Complete genome sequencing of Bacillus thuringiensis subsp. kurstaki
            type strain YBT-1520
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11769)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2013) College of Life Science and Technology,
            State Key Laboratory of Agricultural Microbiology, Huazhong
            Agricultural University, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.0
            Genome Coverage                   :: 1300x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000688795.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 17:49:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,741
            CDSs (total)                      :: 6,598
            Genes (coding)                    :: 6,085
            CDSs (with protein)               :: 6,085
            Genes (RNA)                       :: 143
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 513
            CDSs (without protein)            :: 513
            Pseudo Genes (ambiguous residues) :: 0 of 513
            Pseudo Genes (frameshifted)       :: 286 of 513
            Pseudo Genes (incomplete)         :: 261 of 513
            Pseudo Genes (internal stop)      :: 84 of 513
            Pseudo Genes (multiple problems)  :: 91 of 513
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004863.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     YBT-1520"
                     /mol_type="genomic DNA"
                     /strain="YBT-1520"
                     /serovar="kurstaki"
                     /isolation_source="soil"
                     /db_xref="taxon:570416"
                     /plasmid="pBMB11"
                     /geo_loc_name="China"
                     /collection_date="1990"
     gene            join(11753..11769,1..244)
                     /locus_tag="YBT1520_RS32445"
     CDS             join(11753..11769,1..244)
                     /locus_tag="YBT1520_RS32445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000992628.1"
                     /translation="MNALIDTIFNPVLGWLAQMAKYLSDLSVPASRPLRISDYLGVFAY
                     LYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVKWW"
                     /name="hypothetical protein"
     gene            665..1027
                     /locus_tag="YBT1520_RS32450"
                     /old_locus_tag="YBT1520_33996"
     CDS             665..1027
                     /locus_tag="YBT1520_RS32450"
                     /old_locus_tag="YBT1520_33996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000880242.1"
                     /translation="MLAKTKRKVQPQFEGHRALIIKEEGTCVVEQVISFDSEILETSTA
                     MLPRENELRLLDDNHGYVYHIYNLEKPARIEAENLKMLRRSSALKGIFDFDTGKKPFDI
                     IGFMPWVIIVLLAIFK"
                     /name="hypothetical protein"
     gene            1041..1403
                     /locus_tag="YBT1520_RS32455"
                     /old_locus_tag="YBT1520_34001"
     CDS             1041..1403
                     /locus_tag="YBT1520_RS32455"
                     /old_locus_tag="YBT1520_34001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000116007.1"
                     /translation="MSVQEMLTKDILEEEGSNETIETVQLFQFMKKHAVPLTKKQQMAM
                     LLLNENGLSDISLYIAGMKEEVLPLSEYHKTLEKLTLSDRIKGNAKLSHLLKANANPSL
                     SLSANSAKVVKGNELA"
                     /name="hypothetical protein"
     gene            1400..2002
                     /locus_tag="YBT1520_RS32460"
                     /old_locus_tag="YBT1520_34006"
     CDS             1400..2002
                     /locus_tag="YBT1520_RS32460"
                     /old_locus_tag="YBT1520_34006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024420.1"
                     /translation="MNIMVFEGFLGSGKTFGMSLFAKHYEEKSGCVLYSNYGLIGSKPF
                     VTLDTFHDIAKEKSSILNLDEAHIDLDARSFSSNSVKFFSQLSYYLRKLRCTLFITSPS
                     FDDLDSRIRGITNVLVRVSNDKNYFYYKMYDVQSKRYLKTMRIQKKKAFAIGSKVYDTT
                     AMVSPVQVPDKRQDFMEFLEALKSTAEEYGRQYKHSA"
                     /name="hypothetical protein"
     gene            2073..2264
                     /locus_tag="YBT1520_RS32465"
                     /old_locus_tag="YBT1520_34011"
     CDS             2073..2264
                     /locus_tag="YBT1520_RS32465"
                     /old_locus_tag="YBT1520_34011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000511841.1"
                     /translation="MGILKKKKFREEVKRINKAHGEMREFLDLLMDRYGLDEEEVKNCE
                     VIKHHFDNLDLMFSQMAK"
                     /name="hypothetical protein"
     gene            2882..3088
                     /locus_tag="YBT1520_RS32470"
                     /old_locus_tag="YBT1520_34016"
     CDS             2882..3088
                     /locus_tag="YBT1520_RS32470"
                     /old_locus_tag="YBT1520_34016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000365510.1"
                     /translation="MDLPVCNEKEVTVLSKTLKYLRSDQSNQVKVHKEYLEDRGWIFDD
                     LTYIDSNIYVVYKKEVVKRNKAF"
                     /name="hypothetical protein"
     gene            3100..3519
                     /locus_tag="YBT1520_RS32475"
                     /old_locus_tag="YBT1520_34021"
     CDS             3100..3519
                     /locus_tag="YBT1520_RS32475"
                     /old_locus_tag="YBT1520_34021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000473093.1"
                     /translation="MFKKFYREYIHVLFNIVAMFFYFSWCLVGAEFNLLTLIAIGIALF
                     IFVKYVSMDKKFVNENIDKLKDKKMIFRKYAISGEDIGYAVFTNFLLFILYLGISVYFK
                     PLSVMALLFAFIVNVSFLMLTLRIMSRLSLKNSKV"
                     /name="hypothetical protein"
     gene            3531..3866
                     /locus_tag="YBT1520_RS32480"
                     /old_locus_tag="YBT1520_34026"
     CDS             3531..3866
                     /locus_tag="YBT1520_RS32480"
                     /old_locus_tag="YBT1520_34026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000457469.1"
                     /translation="MFAKYYSQDDEFMSPLQFIALGGIMSFLIKFGCISPSVNMLTLFV
                     TFVTCIGLCYFFLLLITKYFYRFLAKHRDNLHVYLCNKYNEKIANRIGLCIGLVYIVGV
                     LVICLLI"
                     /name="hypothetical protein"
     gene            4165..4377
                     /locus_tag="YBT1520_RS32490"
                     /old_locus_tag="YBT1520_34036"
     CDS             4165..4377
                     /locus_tag="YBT1520_RS32490"
                     /old_locus_tag="YBT1520_34036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000216293.1"
                     /translation="MTVKEKKKTWAFTFTESEIQIIDEIVDIENDRRHSIAREHNLPFK
                     KYNRSTFVLAMIEEKKRKYQEQGEI"
                     /name="hypothetical protein"
     gene            4580..4801
                     /locus_tag="YBT1520_RS32495"
                     /old_locus_tag="YBT1520_34041"
     CDS             4580..4801
                     /locus_tag="YBT1520_RS32495"
                     /old_locus_tag="YBT1520_34041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391857.1"
                     /translation="MEELLHKIIDKLDKIDKDIQEIKTDIKAHRIETDDNFIKLYHLVE
                     EKDIEIDVLNKRLFKNEATVERIKREKH"
                     /name="hypothetical protein"
     gene            4815..5093
                     /locus_tag="YBT1520_RS32500"
                     /old_locus_tag="YBT1520_34046"
     CDS             4815..5093
                     /locus_tag="YBT1520_RS32500"
                     /old_locus_tag="YBT1520_34046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000331888.1"
                     /translation="MAYSYCVDCERVYNCEDEKCCCGSKKRSVDWEEFAFIMGYPLYPV
                     KKKYGVFHLDLSKRLDADALCDLKADNPELFYYEFKGWKERELKKRN"
                     /name="hypothetical protein"
     gene            5420..5611
                     /locus_tag="YBT1520_RS32505"
                     /old_locus_tag="YBT1520_34051"
     CDS             5420..5611
                     /locus_tag="YBT1520_RS32505"
                     /old_locus_tag="YBT1520_34051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000048809.1"
                     /translation="MSKVKGIRLNLRVSEDEYNKLQYVKECLLMEGLDLKDSAVIRYAL
                     LQTYKDYKEMNDLLKSRN"
                     /name="hypothetical protein"
     gene            5798..6868
                     /locus_tag="YBT1520_RS32510"
                     /old_locus_tag="YBT1520_34056"
     CDS             5798..6868
                     /locus_tag="YBT1520_RS32510"
                     /old_locus_tag="YBT1520_34056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410863.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000410863.1"
                     /translation="MEKHIEKLSLSVCVDWLEFTFVYGEEFESICSFLGLDPTVFSKEI
                     DGFHKSYGYLSRYSFEEIHILMHGADNRSRIIMSGSGCRWFETLSSVGWYGLFDRIAFA
                     DEHGFSWIKVDRLDIAIDDFVGYFSVKKLKSKIKRRECLSRWKTSFVVETFDLEQGRLQ
                     GSTIYFGSASSKLRWVVYDKLEEQKKKNKDSDSIQGLEFWTRHELRLKKERADRAVKQL
                     VVNDFSIEEMYFGIINNYISFRVVDKEDSNRNRWNIAPFWRQYIGQASKLRLAEALPVA
                     NVERTFKWLSHSVSKKLYLLSEIFSQDDDTKKEFASYLENKGKQKITKGDLDLIENFPI
                     RDFKEFLKIYNEKCLK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            7029..7334
                     /locus_tag="YBT1520_RS32515"
                     /old_locus_tag="YBT1520_34061"
     CDS             7029..7334
                     /locus_tag="YBT1520_RS32515"
                     /old_locus_tag="YBT1520_34061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001176491.1"
                     /translation="MQLMKVMLTGCKKVDFTNDNNERIEGVNIHFLTTTDNSEQSGFGY
                     LPSKVFFRKEFIEKFSSLNYPIVAEAIIAFNMSPRGIQTKISDFNILEEFPFTALK"
                     /name="hypothetical protein"
     gene            7928..8080
                     /locus_tag="YBT1520_RS35935"
     CDS             7928..8080
                     /locus_tag="YBT1520_RS35935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001113828.1"
                     /translation="MPEFGFRPSMSQLFGNFHMNWSTMMPLLVILFGILFGFFVAHLVK
                     DKFDD"
                     /name="hypothetical protein"
     gene            8101..8259
                     /locus_tag="YBT1520_RS35940"
                     /old_locus_tag="YBT1520_34066"
     CDS             8101..8259
                     /locus_tag="YBT1520_RS35940"
                     /old_locus_tag="YBT1520_34066"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425806.1"
                     /translation="MEMPKITLTYNLADVANGLTNWLGAYWPIIAFSVAIPLTFLVAFN
                     TKDLFTR"
                     /name="hypothetical protein"
     gene            8366..9772
                     /locus_tag="YBT1520_RS32520"
                     /old_locus_tag="YBT1520_34071"
     CDS             8366..9772
                     /locus_tag="YBT1520_RS32520"
                     /old_locus_tag="YBT1520_34071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386301.1"
                     /translation="MKKLAIIFLCAFCIVSHTIPVFADSPSFQLVGSKNVTIENNGIVN
                     GKYNLIIKVNNMKDVYSFQLSELMLVNDCSFHVGKSKWTRPEQEKNEFPINWNQNGTYA
                     FYIFHNGKVLGYARFKLKGFKENGGGGGETVYWGKGAKYYDLPEIEKPNPEDYADYDNK
                     NDGVCTNEKIPDDKPQGSGDIDESKEDNKPPVDGGGGSDKAVKEVLEKIVSAVNEISKN
                     TGETAKNTGETAKNTGEIKKNTDEIAKNTKEIADAVKDIRDNLKIPDDMDLSIKPVLPE
                     DIKPDKPKQPDKPFEDKQEHFKEGEQEKPPDKLPVAPEPAECWGDVCKDKDNQKEEDMK
                     KEKDMDKEEPMKPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPD
                     KPMTPDKPMEKEDMKPDKPMTPDKPMDREGEMKKDNDMKQDKPMTPWEEMKPSDEGSEL
                     CWHVVNGKCG"
                     /name="hypothetical protein"
     gene            9739..11166
                     /locus_tag="YBT1520_RS32525"
                     /old_locus_tag="YBT1520_34076"
     CDS             9739..11166
                     /locus_tag="YBT1520_RS32525"
                     /old_locus_tag="YBT1520_34076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090439.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="WP_228176732.1"
                     /translation="MLACSEWKMRIKKFFIVVPVLLGILFVGDISAFAQGKDVLSGRVG
                     KAQYGSSYITSLTDGNLQTSDTLSNGEVIIDLASPVRVSGFRFTKVGGSSNRMDVYFYT
                     EGATLKREYRNTDSGYLQAIDIKGVKKIGLKNMSNGDYFTLAEVNLYEYESVPPSDITD
                     LKENIKHDSVEFTYKNPKSNFSHLRIYRDGQVLASDVKEEKFTDKGLTPETEHIYKFVS
                     VSPDGLESKGIEKKIKTAKEPLPDEIKDLQEDEITHTYVKFSYKFPKNNFDHVKIYRDD
                     KVIADNVKVEEFVDKGLKGSTVYTYKFVSVSSTGLESNGIVKKVQTDEEPNLNKPSKPS
                     NFDITPKDGSLVVNLLSQNLGVPIKGYHIFIDGVKVNDSLVTSRSYAVKGLQNGQTYGV
                     QIKAVSKWNIESDLSNVVNGIPKVPVIPEVTFSFGLGALVDGITNWFLGIWPIVAFSIA
                     IPLAFIVAFNTKKLFMR"
                     /name="fibronectin type III domain-containing protein"
     gene            11176..11331
                     /locus_tag="YBT1520_RS35945"
     CDS             11176..11331
                     /locus_tag="YBT1520_RS35945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386302.1"
                     /translation="MIQPVTTKDLIQFLQYAFHAGEDVLFLAFVLMITFSVAVSIRRIM
                     VGYKQS"
                     /name="hypothetical protein"
     gene            11328..11753
                     /locus_tag="YBT1520_RS32530"
                     /old_locus_tag="YBT1520_34081"
     CDS             11328..11753
                     /locus_tag="YBT1520_RS32530"
                     /old_locus_tag="YBT1520_34081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000970219.1"
                     /translation="MMEKFVKFLKGGFEAVIKSLKTFIDFLATPFQYLLDFLKGIFYFI
                     EKLFQVVVAIIKLFVALFQFLGAVIAGFFKTLFLWVQPNFDGGNVSFPSASMKGFKVAT
                     DILLPTGLLTVIPAVVTCFIWYLFVKKLMDLFRGGHH"
                     /name="hypothetical protein"
     Typing          5799..6868
                     /name="rep_cluster_814"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5799..6865
                     /name="MOBT"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacaatattt aatcctgttc ttggttggct tgcacaaatg gcaaagtatt taagtgattt
       61 aagcgtaccg gcaagccgtc ctttgcgaat tagtgattac cttggagtat ttgcttattt
      121 atacggttgg acaaccgtta taacgacgtt attcgcttta gttttcgtat attttgtttg
      181 ttacatcatc atgacttaca gtagtttatt tattcgattt aaagatacag tgaaatggtg
      241 gtaatgatat tgaatattgt aggacaagca attttattaa tattttttac gacagttgtt
      301 tcttggatgg taatagatac attgaatttc tttagaggta tagaagaatg atttatgcaa
      361 cgttggggtt tattagttgg attttgattt tgatttgtga agtaacatcg agaaaaggag
      421 atttaaaata atgcaggctt ttaatgcgat gtttgagtta tctagtgcgg tttgtttatc
      481 tgtatttctt tttatagttt taccatatat gtttttcaaa aataaaatgt caaagtgata
      541 aaaacgaatg ttaaatatca attagtttat atagttcgtt aaaaagattc aaaatgtcat
      601 ggcagcggga ctagatccaa gagattttga aatgaaaaag cgaacgtttt ggagggtttt
      661 ataaatgtta gctaaaacaa aacggaaagt acagccacaa tttgaagggc atagagcatt
      721 gattattaaa gaagaaggaa catgtgtagt tgaacaggtt atatcgtttg attcggagat
      781 tttagaaacg agtacagcca tgttaccacg tgagaatgaa ttgagattac ttgatgataa
      841 tcatggttat gtgtatcaca tttacaactt agaaaagcca gcaagaattg aagcggaaaa
      901 tttgaaaatg ttacgtcgtt ctagtgcatt aaagggaatt tttgattttg atacagggaa
      961 gaaacctttt gacattattg gttttatgcc ttgggtaatt attgtattgc tagctatttt
     1021 taaataatgg ggtggagtaa atgagtgttc aggaaatgtt aacgaaagat attttagaag
     1081 aagaaggttc aaacgaaacg attgagactg ttcaactgtt tcaattcatg aaaaaacatg
     1141 ctgtgccatt aacaaagaaa caacaaatgg ctatgttatt gcttaatgaa aatggattat
     1201 cggacatttc tttatatatt gctggaatga aagaagaagt attgccattg agtgaatacc
     1261 acaagacact tgaaaaatta acgctttctg accgaattaa aggtaatgct aaattaagtc
     1321 atttactgaa agcaaatgcg aatccttcat tatcattgtc agcaaatagc gcaaaagttg
     1381 ttaaagggaa tgagttagca tgaacataat ggtttttgag ggctttctcg gtagcggtaa
     1441 aacgtttggt atgagtttgt ttgcaaaaca ctatgaagaa aagagtggct gtgtattgta
     1501 cagtaattac ggtttaattg ggtctaaacc atttgtaact ttagatacgt ttcatgatat
     1561 tgcaaaagag aaatctagta tcttaaatct cgatgaagcg catattgatc ttgatgctag
     1621 aagtttttca agtaactcgg taaagttctt ttctcaattg tcttattacc ttagaaaact
     1681 acgttgtacg ttgtttatta cctcaccatc atttgatgat ttagattcac gtataagagg
     1741 tattacaaac gttctcgtta gggtatcgaa tgataaaaac tatttttatt acaaaatgta
     1801 tgatgttcaa tctaaacgat atttaaaaac aatgcgtatt caaaagaaga aagcttttgc
     1861 tattggttca aaagtttatg atacgacagc aatggtttct ccagtacaag ttccagataa
     1921 acgacaagac ttcatggaat ttttagaagc gttgaagtcg accgccgaag agtacggacg
     1981 ccagtacaag cattcagcgt aagctgtatg cgcggtgcag gcgggaggac ttcataagat
     2041 tatataaatt aaataaggaa ggtacgttat agatgggaat tttaaagaag aaaaaattta
     2101 gagaagaagt gaaaagaata aataaagcac atggagaaat gagagaattt ctagatttgc
     2161 tcatggatcg ctacggatta gatgaagaag aagtaaaaaa ttgtgaagta attaaacatc
     2221 attttgataa tttagatttg atgtttagtc aaatggcaaa ataatgttcg tgatttaggt
     2281 gtgaaatgta acggcagccg tgcatatttt ccaggggctt ttaatgaaaa tacgaacatt
     2341 aagtatgagg tgaagagatt tgatacatgt agattggttg aaaactatgg cgagagacgg
     2401 attattttat ggttcattca ttgctgtaat tgttttcttg ctaaattggt ttacgggtta
     2461 ggttttaaag gtcttccttc gcttttgagg ttggagattg gctggggtgt ggggctagcc
     2521 ccgcatagcg taatgggtaa atagaataaa agaaaatata aaggtcggta gtacaagcgt
     2581 ttaggctcgt ataatggata ggcaagttga tagttaaata tattcacttt tgagtttatc
     2641 gacttggcga tgaagacaaa cgagcctaaa aaagttatgc aagaatgtag cgtagcggaa
     2701 aaggtggaga ttttatgaag cagagcgtaa gaaaatacta cctgtttctt gtttaacctt
     2761 ttagcaaata cagatagagg gttgttttga tggagaatgt tattgttacg ttgtttggta
     2821 ctctagggat ttgtttcttg tttaaaggca tgctagatac tttaaaagaa gggtgatatt
     2881 tgtggatttg cctgtttgta atgaaaaaga ggtaactgtt ttatctaaga cattgaaata
     2941 tttgagaagt gatcaatcta atcaagttaa ggtacataaa gagtatttag aggacagagg
     3001 ttggattttt gatgatttaa cttatataga tagcaatatc tatgtagttt ataaaaaaga
     3061 ggttgtaaag cgaaataagg cgttttaagg ggtgtatata tgtttaaaaa gttttatcgt
     3121 gaatatatcc atgtattgtt taatattgtt gcgatgtttt tttattttag ttggtgttta
     3181 gttggtgctg aatttaattt attaactcta atagcaattg gaattgccct ttttattttt
     3241 gttaagtatg tatctatgga taagaaattt gttaatgaaa atatagataa attaaaagac
     3301 aaaaagatga tttttaggaa atatgctata agtggtgaag atattggata tgcagttttt
     3361 acaaattttt tattgtttat tttgtattta ggaatatccg tatattttaa gcctttaagt
     3421 gtaatggctt tattatttgc ttttattgtt aatgttagtt ttcttatgtt aactcttaga
     3481 ataatgagta gattaagttt aaagaatagt aaagtttaag aggtgtatat atgtttgcta
     3541 agtattatag ccaagatgat gaatttatgt cgccacttca atttatagct ttgggtggta
     3601 ttatgagttt tcttattaaa tttggttgta tttcgcctag tgtgaatatg ttaacgttat
     3661 ttgttacttt cgttacttgt attggattat gttatttttt tctactttta ataacaaagt
     3721 atttttatag atttcttgct aaacatcgtg ataatttaca tgtttattta tgtaataagt
     3781 acaatgaaaa aatagcaaat agaatcggtt tatgtattgg tttagtttat attgtagggg
     3841 tgttagttat atgtttgctg atttaaagtt gaattttaaa ttttggttaa ctgtagtttt
     3901 tgatatatgt atgcttgcaa tgatctgtgt tgttacaaca aagtatgtat ctgatatata
     3961 tagtgatttg tttattttca cgacaggatt tttaatacta ttaatttggt attctgttag
     4021 agtgtatttg atagatttaa tcagaaaaaa acctatcaat aattaaaatt taatattttt
     4081 ttattgaaaa accatatgtt tttatttaca aaccatatgg tttttgttat attaaattta
     4141 gttaattgat aggaggttgt ttttatgaca gttaaagaaa agaaaaaaac ttgggcattt
     4201 acatttactg aaagtgaaat tcagataata gatgaaattg tagatataga gaatgataga
     4261 agacattcaa ttgctagaga acataattta ccgtttaaaa aatataatcg ttctacattt
     4321 gtgcttgcaa tgattgaaga aaagaaacgt aagtatcaag agcaaggaga aatttaatgg
     4381 aaattgtttt aggtgttagt gcgtttggtt tgtatatttc gtttttaata tttttatatt
     4441 ggatggcatt agatttttta gcttggttat ataaaaagat ttaataaaat cgttatttta
     4501 ttggttttct cttaacttgt taatattaat aggggataga gtctaacttt tttaaaatta
     4561 tattttggag gtcgtaacta tggaagaatt gttacataag attattgata aattagacaa
     4621 gattgataag gatattcagg agattaaaac tgatattaag gctcaccgaa ttgaaactga
     4681 tgataatttt attaagctat atcatttagt tgaagaaaaa gatattgaga ttgatgtgtt
     4741 gaataagcgt ttgtttaaga acgaggcgac agttgaacgt attaagaggg aaaagcatta
     4801 ggaggaaatt gtcgatggca tatagttatt gtgtggattg tgagcgtgtg tataactgtg
     4861 aagatgaaaa gtgttgttgt ggaagtaaaa aacgttctgt tgattgggaa gagtttgctt
     4921 ttattatggg ttatccgtta tatcctgtta agaaaaaata cggtgttttt catttagatt
     4981 tatctaaacg tttagatgct gatgctctat gtgatcttaa agcagataat ccagaacttt
     5041 tttattatga gtttaaaggt tggaaagaaa gagaattgaa aaagagaaat taataataaa
     5101 tattttggag ttagtgggtg ttttgtttgt ctaattcgaa ggaaaatttc cagttgcgaa
     5161 atgaggaacc cccgtccagt aacacggggg ttcccggctg ttttgatatc tacactatcc
     5221 cttgcctaga ctgggttcaa gtcactttta aatctgtaca gaattcacaa aaaaatgcac
     5281 ggaaaattat tagtaaaatt tccagttttt aatgaagctt aaaagattct tatattggta
     5341 taatatttac aaggaggaaa atggaaactc tttacatatg gtgtatgaca ttgtatgctt
     5401 caattgagga ggttatataa tgtcaaaagt taaaggtatt cgtttgaatt taagagtttc
     5461 tgaagatgag tataacaagt tgcagtatgt taaagaatgt ttacttatgg aaggtcttga
     5521 tcttaaagat tcagctgtta ttcgttatgc tttattacag acttataagg attataaaga
     5581 aatgaatgat ttgttgaaat caaggaattg aatagctgat ttcatgtttc tactgggggc
     5641 tagtaacacc ccagtagaaa atacaggtca atcacctccg aacgcttgtg gctctagggt
     5701 tcgtccttct ttgttttttt cacggttctt ttaaaaaata ggcaaaaaat tcgcggtttt
     5761 tattttgctt ttattgtatt gttttagagg tgtttgtatg gaaaaacata ttgaaaaatt
     5821 gtctctttcg gtttgtgtgg actggttgga gtttactttt gtttatggtg aagagtttga
     5881 atctatttgt agttttttag gtctagatcc tactgttttt tctaaggaaa tagatggttt
     5941 tcacaagtct tatggttatt tgtctagata ttcttttgaa gaaatacaca ttcttatgca
     6001 tggtgctgat aatcgttcta gaattattat gtctggttct ggttgcagat ggtttgaaac
     6061 attatcctct gttggctggt atggtttgtt tgataggatt gcatttgcag acgaacatgg
     6121 tttttcttgg attaaagttg accgtttaga tattgctatt gatgattttg taggttattt
     6181 ttctgttaaa aaattaaagt ctaaaataaa aagaagagaa tgtttgagtc gttggaaaac
     6241 aagttttgtt gttgaaactt ttgatttaga acagggacga ttgcagggtt ctacgattta
     6301 ttttggaagt gcttctagta aattgagatg ggttgtttat gataagttag aagagcagaa
     6361 aaaaaagaat aaagatagtg attcgattca aggtttagaa ttttggacta ggcatgagtt
     6421 gcgtttgaaa aaggagcgtg ctgatcgtgc tgttaaacaa cttgtagtta atgattttag
     6481 tattgaagaa atgtattttg gtattataaa taattatatt tcgtttcgtg ttgtcgataa
     6541 ggaagattct aatagaaata ggtggaatat tgcacctttt tggaggcaat atataggaca
     6601 agcaagtaaa ttaagattag cagaggcttt acctgttgca aatgttgagc gtacgtttaa
     6661 atggttgtct catagtgttt ctaaaaaatt atatttgttg tctgaaattt tttcgcaaga
     6721 tgatgataca aagaaagagt ttgctagtta tttagaaaat aagggcaaac aaaaaatcac
     6781 gaaaggggat ttagacttaa tagaaaattt cccaattcgt gacttcaaag aatttttaaa
     6841 gatttataat gaaaaatgtc taaaataatt ttactctatt ttataatgga agtctaatac
     6901 attaaaaaat ccatatattt ttaaaaaaag actagaattt ttttctagtt tttcttataa
     6961 ttaaatgtat gacaaaggtg tacatagaac gacattgtat gactaaaaag attctaagga
     7021 gtgtaaatat gcaattaatg aaagtaatgt taacaggttg taagaaagtt gatttcacaa
     7081 atgataataa tgaaagaatc gaaggtgtta atattcactt tttaactaca actgataata
     7141 gtgagcaatc tggttttgga tatcttcctt ctaaggtctt ttttagaaaa gagtttatag
     7201 aaaaatttag ttctttgaat tatccaattg ttgctgaagc tattattgct tttaatatgt
     7261 ctcctcgtgg tattcaaact aaaatttctg attttaatat tttagaggaa tttcctttta
     7321 cagctttaaa gtgattttat gatagataaa atcgatttaa atgaattgta caaaactatt
     7381 tcggaaatta tgcctaatta cttagtgcag actaagaatg gtagttttgc agttttccag
     7441 acacttacct atggtgatat gttaattagt ttcttgttat tgctaatctt gttggtgttg
     7501 acattcaact ggatatggag tgctttacga tgatcgagtt taatttagaa aacttgttga
     7561 atcaatattc cttgtttgga ttaccatttt tattaattat cgtgttcaca ttgctgaaag
     7621 attggcggtt tgatagataa tgaatgattt agcgtttcta gatatgttgt attggttctt
     7681 catgaacaat tcagttgttt tatactcgtt aggttttata acctttctag tattcgtaat
     7741 aatgatgttc accaccgtca aagcttgggg tgatggctat tagttttagc atggacgtgg
     7801 gaagaatctt acagtatgcc tttaacatgt ttagttcggt gttaccagtg atttatttat
     7861 tcattggtgg ggcgtttgct atctactgta tcggtaaagt gtacaacacc tttaggagtt
     7921 gatttcaatg cctgaatttg gattcagacc atcgatgtct cagttattcg gtaactttca
     7981 tatgaactgg tcaacaatga tgccattgtt agtcattttg tttgggatac tgtttggatt
     8041 tttcgtagcg catttagtaa aagacaaatt tgatgattga ttaattagga ggaatttatt
     8101 ttggaaatgc caaaaattac attgacttat aatcttgcag acgttgctaa cggtttgact
     8161 aactggttag gagcatattg gcctattatc gcattctcgg ttgcgattcc attaacgttc
     8221 ttagtggcgt ttaacactaa agacctattt actcgttaat catcaaataa tcactcctta
     8281 aatagcaaga agttaactat aaatttaata aaacaattaa atagctgggc taataaccta
     8341 gctatttttg ttaggaagga ttgatatgaa aaagctagcc ataatttttt tatgtgcatt
     8401 ttgtattgtt agccatacga tccctgtgtt tgctgattca cctagttttc aattggtagg
     8461 tagtaaaaat gtaacaatcg aaaataatgg gatagttaat ggtaaataca atttaattat
     8521 taaagttaac aatatgaaag atgtatatag ttttcagtta tcggaattga tgcttgttaa
     8581 tgattgctcg ttccatgttg gtaaatcaaa gtggactcgt ccagaacaag aaaaaaacga
     8641 gttccctatt aactggaatc agaatggtac atacgcattt tacatttttc ataatggtaa
     8701 agttttaggc tatgcacgtt ttaagcttaa aggatttaaa gaaaacggtg gcggtggagg
     8761 agaaactgta tattggggca aaggtgctaa atactacgat ttacctgaaa ttgaaaagcc
     8821 taatcctgag gactatgcgg attatgataa taagaatgac ggagtttgta caaatgaaaa
     8881 aattccagat gataaaccac aaggcagcgg tgatatagat gaatcaaaag aagataataa
     8941 accacctgta gatggtggcg gtggttctga taaagctgtt aaagaagttt tagaaaagat
     9001 tgttagtgca gtaaatgaaa tttcaaagaa tacaggtgaa acggctaaaa atacaggtga
     9061 gacagcaaag aatacaggtg agattaaaaa gaacaccgat gaaatagcaa agaacacaaa
     9121 agaaattgcg gatgctgtta aggatattag ggataatttg aagattcctg acgatatgga
     9181 tttgtcgatt aaacctgttc tacctgagga cattaagcct gataaaccta aacaacctga
     9241 taagcctttt gaagacaaac aagagcactt taaagagggt gaacaagaga aaccacctga
     9301 caaacttcct gttgctcctg agcccgctga atgttggggg gatgtttgta aagataaaga
     9361 taatcagaaa gaagaagata tgaagaaaga aaaagatatg gataaagaag aaccaatgaa
     9421 gcctgataaa ccaatggaaa aggaagacat gaaaccagat aagccaatga ctcctgataa
     9481 gccaatggaa aaggaagaca tgaagcctga taagccaatg actcctgata agccaatgga
     9541 aaaggaagac atgaagcctg ataaaccaat gactcctgat aaaccaatgg aaaaggaaga
     9601 catgaaacct gataaaccaa tgactcctga taagcctatg gatcgggagg gagaaatgaa
     9661 aaaagataat gatatgaaac aagataagcc aatgactcct tgggaagaaa tgaaaccaag
     9721 tgacgagggg agtgaacttt gttggcatgt agtgaatgga aaatgcggat aaagaaattt
     9781 tttatagtag tacctgtatt acttgggatt ttgtttgtag gtgatataag tgcatttgct
     9841 caaggtaaag atgtattaag tggaagagta ggtaaagctc aatatggtag ttcatatata
     9901 acttcactaa ctgatggaaa tttacaaact agtgatactc tttctaatgg tgaggtcata
     9961 attgatttag cttctcctgt tagagtttca gggtttaggt ttactaaagt tggtggctct
    10021 agtaatagga tggatgttta tttttataca gagggtgcta ctttaaagcg ggaatataga
    10081 aatactgata gtggttatct acaagccatt gatattaaag gtgttaaaaa aattggattg
    10141 aaaaatatga gcaatggaga ttattttaca cttgctgaag ttaatttata tgagtatgaa
    10201 tctgttcctc catctgatat tacggattta aaagaaaata ttaaacatga ttcagttgaa
    10261 tttacttata aaaatccgaa gtcgaatttt agtcatttaa gaatttatcg tgatggacaa
    10321 gtgcttgcta gtgatgtgaa agaagaaaaa ttcactgata aaggtttaac tcctgaaacg
    10381 gagcatattt ataaatttgt ttcagtttct cctgacggtc ttgaatcaaa aggtatagag
    10441 aaaaagataa agacagcgaa agagccttta ccagatgaaa taaaagattt acaggaagat
    10501 gaaataactc atacttatgt gaaatttagt tataagtttc ctaaaaataa ttttgatcat
    10561 gtgaaaattt atcgtgatga taaggtgatt gcggataatg tgaaggttga ggaatttgtt
    10621 gataaaggtc taaaaggaag tactgtatat acttataaat ttgtttcagt ttcctctact
    10681 ggtcttgaat caaatggaat tgtgaagaaa gtacagactg atgaagaacc taatctcaat
    10741 aagccttcta aaccttcgaa ttttgatatt acgccaaaag atggctcgtt agttgtaaat
    10801 ctattaagtc aaaatttagg cgttccaatt aagggttatc acatttttat tgatggtgta
    10861 aaagttaatg attcacttgt tacaagtcgt tcttatgctg ttaaaggttt acagaatggt
    10921 caaacttacg gtgtacagat taaggcggtt tctaagtgga atattgaaag tgatttaagc
    10981 aatgttgtta atggaattcc taaagtacca gttattcctg aggttacctt tagttttggt
    11041 ttaggtgctt tagttgatgg gataacaaat tggttcctgg ggatctggcc gattgtggca
    11101 tttagtattg ctattccatt agcgtttatt gttgcgttta atacgaaaaa gttgtttatg
    11161 aggtaggtgc ttagtttgat acaaccagtt acaactaagg acttaataca atttctccag
    11221 tatgcgttcc atgctgggga agacgtatta tttctagcgt ttgtattaat gattacattc
    11281 tctgttgctg taagcattag aaggattatg gtgggttata agcaatcatg atggagaagt
    11341 ttgttaagtt cttaaaaggt ggatttgaag cggttataaa gtcgttaaaa acgtttattg
    11401 attttttagc tacaccattt caatatctac tggatttttt gaaaggaatc ttttatttca
    11461 tagaaaagct gtttcaggtt gtagtagcaa ttataaaatt attcgttgcc ttgtttcagt
    11521 ttttaggtgc tgtaatagcg ggattcttta aaactttatt tctatgggta caacctaatt
    11581 ttgatggtgg gaatgtatcg tttcctagtg catcaatgaa aggttttaaa gtagctactg
    11641 atatattact tcccactgga ttacttacag ttattccggc tgtggtcaca tgttttattt
    11701 ggtacttgtt tgttaaaaag ctcatggatc tattcagagg tggtcatcat taatgaatgc
    11761 gttaattga
//
