LOCUS       NZ_CP004859             2062 bp    DNA     circular CON 10-APR-2024
DEFINITION  Bacillus thuringiensis serovar kurstaki str. YBT-1520 plasmid
            pBMB2062, complete sequence.
ACCESSION   NZ_CP004859
VERSION     NZ_CP004859.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02776149
            Assembly: GCF_000688795.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar kurstaki str. YBT-1520
  ORGANISM  Bacillus thuringiensis serovar kurstaki str. YBT-1520
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Complete genome sequencing of Bacillus thuringiensis subsp. kurstaki
            type strain YBT-1520
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Zhu,L. and Sun,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAR-2013) College of Life Science and Technology,
            State Key Laboratory of Agricultural Microbiology, Huazhong
            Agricultural University, Wuhan, Hubei 430070, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.0
            Genome Coverage                   :: 1300x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000688795.1-RS_2024_04_10
            Annotation Date                   :: 04/10/2024 17:49:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,741
            CDSs (total)                      :: 6,598
            Genes (coding)                    :: 6,085
            CDSs (with protein)               :: 6,085
            Genes (RNA)                       :: 143
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 99
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 513
            CDSs (without protein)            :: 513
            Pseudo Genes (ambiguous residues) :: 0 of 513
            Pseudo Genes (frameshifted)       :: 286 of 513
            Pseudo Genes (incomplete)         :: 261 of 513
            Pseudo Genes (internal stop)      :: 84 of 513
            Pseudo Genes (multiple problems)  :: 91 of 513
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP004859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bacillus thuringiensis serovar kurstaki str.
                     YBT-1520"
                     /mol_type="genomic DNA"
                     /strain="YBT-1520"
                     /serovar="kurstaki"
                     /isolation_source="soil"
                     /db_xref="taxon:570416"
                     /plasmid="pBMB2062"
                     /geo_loc_name="China"
                     /collection_date="1990"
     gene            284..511
                     /locus_tag="YBT1520_RS29155"
                     /old_locus_tag="YBT1520_34296"
     CDS             284..511
                     /locus_tag="YBT1520_RS29155"
                     /old_locus_tag="YBT1520_34296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002100596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000572905.1"
                     /translation="MIESEGNNLKLTERQLNDLKRISELRVKLFGVPGESVVDPENVEF
                     LLDNAISSYLGQLEIFEVTIEIEQYNSMCG"
                     /name="hypothetical protein"
     gene            612..1481
                     /locus_tag="YBT1520_RS29160"
                     /old_locus_tag="YBT1520_34301"
     CDS             612..1481
                     /locus_tag="YBT1520_RS29160"
                     /old_locus_tag="YBT1520_34301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198524.1"
                     /translation="MQVYLDRLMIKYKDVTEKQFSDVLTKISSKQIFLPNTPIRSEHGT
                     SVRDYHRVIHIGYGEGAVYIGWKHNSEKEKDSYDMKVDFNPSKFENNELQKDSYEKVFE
                     TVFHTLNAVLKSNKRVVYGMDIAFDIERHMSDIVSYSKTGKQQDRHKGTVYYGNRNKDG
                     YLKIYDKKKELYNHFKRMIEEENLTRIEYSWRDSDGVVVDEIRKSPPFSIDESYTFSIF
                     NLNNVKGALKACLICYSNGTMDMKEFPRRTKESIKKALEEMDHLAVDPILQDCWLSILE
                     NIKNYTRL"
                     /name="hypothetical protein"
     gene            1669..1911
                     /locus_tag="YBT1520_RS29165"
                     /old_locus_tag="YBT1520_34306"
     CDS             1669..1911
                     /locus_tag="YBT1520_RS29165"
                     /old_locus_tag="YBT1520_34306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001293418.1"
                     /translation="MSEMVRVNTRISKKLNDWLDEYSKESGVPKSTLVHLALENYVNQK
                     VMLEQMPKMQQMLSMMFENVTQQQLNQKGNMFELK"
                     /name="hypothetical protein"
     Typing          613..1481
                     /name="rep_cluster_1434"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgtatgagt tgtaattatt attcacttgg gtgaacgttg tatgactgaa aggaaaccca
       61 tggcagttaa ctgtgtaaat gggcacttat ttattaagtg tattcgacgg agccggaagt
      121 agacgactta agggagcgac atgaatgaag tgaagtgcag acagaaggta aggtgacgga
      181 gtggactctc aaaggacacg acggcgcgtg aactgatggc aaacgaaatg gaatgagtga
      241 gaagagcgta cggtcggcga ggtaacggag gtgtaggagc agattgatag aaagtgaggg
      301 taacaatttg aaactgacag aaagacaatt gaatgatttg aaaagaatta gcgaattacg
      361 tgtaaagttg tttggagttc ctggtgaaag tgtagttgat ccagagaatg ttgagttttt
      421 attggataat gctattagtt cttatttagg gcaattagaa atttttgaag tcacgataga
      481 gattgaacag tataattcaa tgtgtgggta aattgtagaa atgtggcgaa gacattttcg
      541 gacattctaa tagccgaaaa tcgtgtacaa aatgacatgt ttaataaaaa ataaggagcg
      601 ggatagattt tatgcaagtt tatttggata ggctaatgat taagtataaa gatgtaacag
      661 agaaacaatt tagtgatgtt ttaactaaaa tatcgtcaaa gcagattttt ttaccgaata
      721 cacctattag gtcagaacat gggacgtctg ttagagatta tcatagagtt atacatattg
      781 gatatggtga aggtgcagtt tatatagggt ggaaacataa ttcggaaaag gaaaaagata
      841 gctatgatat gaaagttgat tttaaccctt ctaaatttga aaataacgag ttgcaaaaag
      901 atagttatga aaaagtgttt gaaaccgttt ttcatacgtt aaatgcagtt ttgaagtcta
      961 ataagcgagt ggtttatggt atggatattg cttttgatat agagcgtcat atgagtgata
     1021 ttgtgtctta tagtaaaaca ggaaagcaac aggatagaca taaaggaact gtttattatg
     1081 gaaatagaaa taaagatgga tatttgaaga tatatgataa gaaaaaggag ttatataatc
     1141 attttaaaag aatgatagaa gaagagaatt tgactcgtat tgagtatagt tggagagact
     1201 ctgacggtgt agtggtagac gaaataagga agagtcctcc gtttagtatt gatgaatctt
     1261 atacattctc gatttttaat ttgaataatg ttaaaggggc attaaaagct tgtttgattt
     1321 gttattctaa tggaactatg gatatgaaag agttccctcg tagaactaaa gagagtataa
     1381 aaaaagccct tgaagaaatg gatcacttgg cggtggaccc cattctacag gactgttggt
     1441 tatctatatt agaaaatatt aagaactata ctcgtttatg atattagcgt gtgcttctct
     1501 gtgtgtcaag agggtgtcaa tatgatgctc tctttttgtt tttcttaact tgtttatatt
     1561 aatagcggat agagtcccac ttttacattg ttctggtgta tcttagtgtt gatattgtgt
     1621 ttaaactgat gttatattta tgtagtacga tatacaagag gtgattagat gagtgaaatg
     1681 gttcgtgtta atacacgtat cagtaaaaag ttaaatgatt ggttggacga gtatagcaaa
     1741 gaaagtggtg taccgaaaag cactttagtt catttagctt tagagaatta tgtgaatcaa
     1801 aaggttatgt tggaacaaat gccaaagatg caacaaatgt tgagtatgat gtttgaaaat
     1861 gtaacgcagc aacaattgaa tcaaaaaggg aatatgtttg agttgaagta acggttatgt
     1921 ttcgaaaatg tagtcttatt gattaggaga tgcacatcga taaactaaat gcgtagttgg
     1981 tgtggctgaa gtttgcccgc cacctactca tttagaatat ccgtgcatgg gtccctgaac
     2041 aattaggaaa cggcttttgt aa
//
