LOCUS       NZ_CP003996             2459 bp    DNA     circular CON 03-MAR-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae Kp13 plasmid pKP13a,
            complete sequence.
ACCESSION   NZ_CP003996
VERSION     NZ_CP003996.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02641520
            Assembly: GCF_000512165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae Kp13
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae Kp13
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ramos,P.I., Picao,R.C., Almeida,L.G., Lima,N.C., Girardello,R.,
            Vivan,A.C., Xavier,D.E., Barcellos,F.G., Pelisson,M., Vespero,E.C.,
            Medigue,C., Vasconcelos,A.T., Gales,A.C. and Nicolas,M.F.
  TITLE     Comparative analysis of the complete genome of KPC-2-producing
            Klebsiella pneumoniae Kp13 reveals remarkable genome plasticity and
            a wide repertoire of virulence and resistance mechanisms
  JOURNAL   BMC Genomics 15, 54 (2014)
   PUBMED   24450656
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Perreira,P.I., Picao,R.C., Almeida,L.G.P., Vespero,E.C.,
            Pelisson,M., Vivan,A.C.P., Zuleta,L.F.G., Barcellos,F.G.,
            Lima,N.C.B., Xavier,D.E., Girardello,R., Medigue,C.,
            Vasconcelos,A.T.R., Gales,A.C. and Nicolas,M.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2012) Labinfo, LNCC - Laboratorio Nacional de
            Computacao Cientifica, Rua Getulio Vargas 333, Petropolis, RJ
            25651070, Brazil
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 06:16:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,644
            CDSs (total)                      :: 5,523
            Genes (coding)                    :: 5,383
            CDSs (with protein)               :: 5,383
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 63 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 22 of 140
            Pseudo Genes (multiple problems)  :: 19 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003996.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Klebsiella pneumoniae subsp. pneumoniae Kp13"
                     /mol_type="genomic DNA"
                     /strain="Kp13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:1123862"
                     /plasmid="pKP13a"
                     /geo_loc_name="Brazil"
                     /collection_date="2009"
     gene            145..1113
                     /gene="mobV"
                     /locus_tag="KP13_RS00060"
                     /old_locus_tag="KP13_06797"
     CDS             145..1113
                     /gene="mobV"
                     /locus_tag="KP13_RS00060"
                     /old_locus_tag="KP13_06797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1212..1442)
                     /locus_tag="KP13_RS00065"
                     /old_locus_tag="KP13_05747"
     CDS             complement(1212..1442)
                     /locus_tag="KP13_RS00065"
                     /old_locus_tag="KP13_05747"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_140430389.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     gene            complement(1429..1911)
                     /locus_tag="KP13_RS00070"
                     /old_locus_tag="KP13_06798"
     CDS             complement(1429..1911)
                     /locus_tag="KP13_RS00070"
                     /old_locus_tag="KP13_06798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          137..1110
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ttttgacctt ctggcagctt gctgcccgaa cgattttgcg tagcaaagtc tagtgagtac
       61 actatcatct tgatagtggc ccgccggagg tatcgcaatg cgctcccccg cctggctgaa
      121 tgaatgccgg aatcaggggg gaacatggcg aactacgcga taatgcgctg caaaaagctg
      181 acgggaatgg gaagcgtggc cagcgcgttg cagcactgct accgagagcg tgagacgccg
      241 aacgctaacg ctgaacggac accagaaaac tactgctctg tgtccaaatc aacggatcag
      301 gcaatggggc gtgtgcggga gctgctgccg gaaaaacgcc gtaaagatgc ggtactggcc
      361 gttgaatacg ttatgacagc aagccctgag tggtggaaag aggccacacc acggcagcag
      421 gcggagtttt tcgcccgctc tgagcaatgg ctggagaaga aatacgggaa ggatcgtgtt
      481 gtggcggcgg tggttcaccg ggacgaggcc accccgcacc tgtctgcgtt cgtggtgcca
      541 ttgacgcagg atggacggct gtccgcgaaa gagtttattg gcgggcggtc aaagatgcgt
      601 gaagatcaga gcacctacgc tgaatcagta aaaaaactgg gtcttgagcg cggcatcgag
      661 ggaagccgcg ccacgcatca gacagttcag cactactacg aatcgataaa ccgtggcacc
      721 cgtagccagg tatcgatctc gccggaggcg ctggagccgc gtgtattgcg gaagggcatt
      781 ttcaccaaag acgtggaaga tcaggcagcc atagccaaac gtctaagcca cgcagtgaac
      841 gatggctttg caggaaccat cgctatggcc tctcagagcg cccagaacgc aaaaagagcg
      901 cgtgagctac agaagactat ggatgcgcag cagaaacgcc tacagagcgt tacagagcct
      961 tttaaggggc tttccagaga gcagatgacc gaaatcctga tgatggctca gcggtttaag
     1021 cagcaaaacc aggaaaagga aaaacagcag cgtattgaac gtgaaaaaca gcgacagacg
     1081 cgatcgcgtg gcatgggtgg tatggagcgt tagttttgat gcgatgcagg atagggctaa
     1141 cgccgcaaag tgacgtcatt tgcggcattc cagagatcag gttctgccgc cctgtataac
     1201 gctgaggcca gctaatccat cagatggtaa ttgtattttt tcaaaataga aaaagctgcg
     1261 tttttctgct ttggtgacaa acccttagtt gagtatttat taccgctagt tctacgatat
     1321 atgctgtaaa cactgcttaa aaaagtacgc tcccaaacag tcagaacctc attctttttt
     1381 agtgaatcat gcagcattag cctgtctaca tttttaaaaa gtttatcttc agcattcatc
     1441 atccattacc ttcgtctgca ccgttcttcc attggtgtca taactgatct ttaactcaat
     1501 tttctttctt ctttcgtaaa ttgatggcca gtctccccgt gcaaaaagaa aggggttagc
     1561 ttcaagttcc ccacgccctg cccggaagaa tatttgttta tctacaagag ccgcaattat
     1621 gtttttaaag gtctgatatt caatatgaac cctgggctgt atggtttttt caaatctagt
     1681 ttttgataca gacacaaacc catcccatcc aatcagcatt gccaattgta aaagcacatc
     1741 attttgagat tttgataaac catgaatcat cgcaatgtct gcaatgtata gcttgacgta
     1801 gggaggttcg gctgggatgg aaaaggtctc gatcttagag tcttgtttta cctcaccgtt
     1861 ttcatccatg tatgtatgcc gttcaagcct agaaatcttt ttcgtattca ctccagcccc
     1921 acgtttcgcc atgatttagt actcgcattt aagtactaaa gagacataca gtcaagtcct
     1981 ttgcatcaag tacgattagt acttgaggtg agtacgatta gtccttgagg tgagtacgat
     2041 tagtacttga ggtgagtacg attggtactt gaggtgagta cgattcttcg ctctagccct
     2101 tggtacaagc gggttcccat atctatatct atatattaag agggaaaata aaggctgaaa
     2161 aacgccattt ttaaacatcg aaaaacgagc ttttttgagt ctggagcgta cggatttcta
     2221 tgtccgggga gtggaaattc cgcttcctcg aacggacaga ctcgcggcga tgccgctccc
     2281 taatccgttc ttgcggcgag cggttcctgc tctttcaaaa acgatattca ttgcctgatt
     2341 tcgtcagggg cgagcgaagc gagtgccatt tacccttgac gactcaccgg ataaaacagg
     2401 cttggcagtg gtggggggtg gcgcggagcg acgccttcca cctgccagac tgccgttga
//
