LOCUS       NZ_CP003994             3223 bp    DNA     circular CON 03-MAR-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae Kp13 plasmid pKP13b,
            complete sequence.
ACCESSION   NZ_CP003994
VERSION     NZ_CP003994.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02641520
            Assembly: GCF_000512165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae Kp13
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae Kp13
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ramos,P.I., Picao,R.C., Almeida,L.G., Lima,N.C., Girardello,R.,
            Vivan,A.C., Xavier,D.E., Barcellos,F.G., Pelisson,M., Vespero,E.C.,
            Medigue,C., Vasconcelos,A.T., Gales,A.C. and Nicolas,M.F.
  TITLE     Comparative analysis of the complete genome of KPC-2-producing
            Klebsiella pneumoniae Kp13 reveals remarkable genome plasticity and
            a wide repertoire of virulence and resistance mechanisms
  JOURNAL   BMC Genomics 15, 54 (2014)
   PUBMED   24450656
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Perreira,P.I., Picao,R.C., Almeida,L.G.P., Vespero,E.C.,
            Pelisson,M., Vivan,A.C.P., Zuleta,L.F.G., Barcellos,F.G.,
            Lima,N.C.B., Xavier,D.E., Girardello,R., Medigue,C.,
            Vasconcelos,A.T.R., Gales,A.C. and Nicolas,M.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2012) Labinfo, LNCC - Laboratorio Nacional de
            Computacao Cientifica, Rua Getulio Vargas 333, Petropolis, RJ
            25651070, Brazil
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 06:16:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,644
            CDSs (total)                      :: 5,523
            Genes (coding)                    :: 5,383
            CDSs (with protein)               :: 5,383
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 63 of 140
            Pseudo Genes (incomplete)         :: 77 of 140
            Pseudo Genes (internal stop)      :: 22 of 140
            Pseudo Genes (multiple problems)  :: 19 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP003994.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae subsp. pneumoniae Kp13"
                     /mol_type="genomic DNA"
                     /strain="Kp13"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:1123862"
                     /plasmid="pKP13b"
                     /geo_loc_name="Brazil"
                     /collection_date="2009"
     gene            complement(98..430)
                     /locus_tag="KP13_RS28635"
                     /old_locus_tag="KP13_32244"
     CDS             complement(98..430)
                     /locus_tag="KP13_RS28635"
                     /old_locus_tag="KP13_32244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(598..813)
                     /locus_tag="KP13_RS30670"
     CDS             complement(598..813)
                     /locus_tag="KP13_RS30670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(817..1302)
                     /locus_tag="KP13_RS30675"
     CDS             complement(817..1302)
                     /locus_tag="KP13_RS30675"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1337..2041)
                     /locus_tag="KP13_RS33005"
                     /pseudo=""
     CDS             complement(<1337..2041)
                     /locus_tag="KP13_RS33005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1980..2303)
                     /locus_tag="KP13_RS00015"
                     /old_locus_tag="KP13_06790"
     CDS             complement(1980..2303)
                     /locus_tag="KP13_RS00015"
                     /old_locus_tag="KP13_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(99..430)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(2400..2591)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          2571..3054
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(501..1990)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcggtggttt ttttgtttac agggaaaaag attacgcgca gaaagaaagg atctcaagga
       61 aatccttttg ctttggccaa tatgcttcgg taaaaagtta atgcgcagaa gattttgaaa
      121 aaatattaat aaccaaaact ccaacgcaaa taaggaacat tcctgcaatt gcgggccaat
      181 caagtttttg accgctaacc aaccaaccag ctaggcttat tagtacaatt cccgcaccag
      241 accaaattgc gtaagcaata ccggtaggta tattttccaa tgtttgggaa agcagataaa
      301 aggcagcgca ataacttatt gcagttccta cagacggcca caggcgtgta aatccttctg
      361 aatatttcat aaaggtggtg cctatgactt cagccacaat agcccctccc aggaaaatat
      421 atgtattcat aaaaatacct catgttgaaa aatttaaata tctttacata acaactaatt
      481 aatagaaccc ggcttatcat agcgaaggac catgatgctg ccactgcgat cgttccagct
      541 ccagcgcttt ctggtgctgc accgcccgct cctgctgccg gtgagtttct atcgcgttca
      601 gcctttcgac aaccggcgca gctgctcgct caaactctgc acctgcctgc tcaagaccgt
      661 gacgcgctcg ttcagcgccg cgttctcccg ttgaataaga ccagacatcg tccgccattc
      721 cgcgaaggcg ttctcccact cgtccagcct tttcgagtag tcctgctgta gctgctctaa
      781 tgcgctcagc aactgttttt ccagctccgt catgtgctat ttccccgcca gtatcatcca
      841 gctcgagtcc tctccgaact gtcccgcttc cgggttcacc ctcacgcagc ggcgtctctg
      901 ctcgccgcag gtcgagagct gtacgccgct gttcctctct gacagcatgg cctgcgtgct
      961 cttctgctcc cggatggtcg tatagttctc gagtatctgc tgcccctgcc accacagaat
     1021 gcccgcgctc gacgctatca gcagcgcgga gaccagaacg atggtcagcc acgtctggct
     1081 gaccatgcgc agcatccctt tcgtgcgctg gctcatggct gaggacagct tccggtcgtg
     1141 gtcgaggatg gcggcgctga ttctcttctc gctttcgctc agttccgctc tgacagactt
     1201 ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta gtctgctgct gcgctttcga
     1261 tttctgctct aagtccttcg ccagcgttaa aagactgttc atagatggct cccttcagtc
     1321 ggatgtttcg ccccccgtcc gggtcggcaa tgctgatgct gctttttgtg gtgcgcacca
     1381 cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg acgcgttttc agctccccgg
     1441 atgaggcaag ggcaagcagg ccgctcgtaa tcgcctgggc ggcttcctgc ttcgcttccg
     1501 gcagcgcgga aggcgagacc agcacccgcc ggttctccgg cgcgttcggg tcatgcagcc
     1561 ccagcctgcc gttcactatg gtctgccagg catcgatgcg cggacggtcg gcgcggtcgt
     1621 aatacggctg tagccgcttg ccggtcagaa gctccgtgtt cgggatcagg aagttcagct
     1681 ccagccgccc cttgtcccgg tgctcaaccc acagcacgct gtactggtct ttatcgagtc
     1741 cgggcatcag aacccgctcg aaactcgcca tcagcttttc gcgctggccg ggcggtaaat
     1801 cctgttcggc aaaagacaga acgccggaag tgtacttttt ggcgtagggc gaggcgtcga
     1861 taagctcccg gacttcatcc ggcttcccct gcagaacgct ggcgccgtcg cgctggcgat
     1921 ctttacccag cagataatcg accggcccgc cgccgccgcc gcgcccccga ggatgaaact
     1981 taacgatcat catcagcacc cttttccagt acggcatgcc gcaaccgctc gagtccggca
     2041 tcgatggcca tcagcgcggc gacaatctgc acgcggtcgt gtccgcttcc accaccggcg
     2101 ttaacctggc gggcaatctg gttaaggttg ttccccatgc cggcaagctg acgaagcagc
     2161 gccggcgaga tcgacggaag tttgccggcg cgtgcgggct tctcgtccag gcacgtctgc
     2221 cgcatccagg ccgcgagttg cttaccctcg cagcgctcca gcagacgccg gtgctcgtcc
     2281 tcagtcaccc acatggtgag cattttgctg cgtttgtctg ccaatccggc ctcctgataa
     2341 cgccgggacg gacgggaaat cttttcccgg tcggcacggt gttggacaag ccgcaggcgc
     2401 ggcagtggtt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
     2461 gtgtatactg gctaaacatg ttatgacgga gtggcaattt atgaaagtgc ttcatgtgac
     2521 aggggaaaaa tgcggctgcc gggcgtaagt gcaaaatgca ggtggtgatg aaatgacgcc
     2581 tcctcgctca ctcggtcgct acgctcctgc cgtgagactg cggcgggcgt taccagctca
     2641 cagattacgg gataagcaaa tgccgctcga agcaggaaag gccaagaaaa ggatgcggag
     2701 ttgccgtttt tccataggct ccgcccccct gacgagtatc acgaaaatga cgctcaaatc
     2761 agcggtggcg aaacccgatg cggcttaaag ataccaggcg tttccccctg gtggctccct
     2821 cgtgctcccc cgcattcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     2881 gtttctcatt ccacgcctga aactcggttc cgggtaggca gttcgctcca ggatggactg
     2941 tatgcacgaa ccccccgttc agtccgacta ccacgcccgt tccggtaact atcaacttga
     3001 gtccaacccg gaaagacacg acaaatcgcc agtggcggta gccattggta actgggttca
     3061 cggagcagag attgtagaga atgcctacag agttcttgaa gtgaaagcct gagtgcggct
     3121 acactgaaag gacagtttag gtatctgcgc tccgtgaaag ccagttacca tcggaacaaa
     3181 gagttggtag ctctgaattc cggcaaacga accaccgttg gta
//
