LOCUS       NZ_AP027984             2089 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain VE-V11-M plasmid pVE-V11-M_6, complete
            sequence.
ACCESSION   NZ_AP027984
VERSION     NZ_AP027984.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00587241
            Assembly: GCF_030162195.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Whole genome sequencing of colistin-resistant Escherichia coli
            isolated from Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 1249x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 18:09:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,363
            CDSs (with protein)               :: 4,363
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 350
            CDSs (without protein)            :: 350
            Pseudo Genes (ambiguous residues) :: 0 of 350
            Pseudo Genes (frameshifted)       :: 214 of 350
            Pseudo Genes (incomplete)         :: 157 of 350
            Pseudo Genes (internal stop)      :: 52 of 350
            Pseudo Genes (multiple problems)  :: 65 of 350
            Pseudo Genes (short protein)      :: 4 of 350
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027984.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="VE-V11-M"
                     /db_xref="taxon:562"
                     /plasmid="pVE-V11-M_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.95329501 N 105.83197885 E"
                     /collection_date="2020-07-03"
     gene            complement(313..579)
                     /locus_tag="QQK36_RS24155"
                     /old_locus_tag="VEV11M_46870"
     CDS             complement(313..579)
                     /locus_tag="QQK36_RS24155"
                     /old_locus_tag="VEV11M_46870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(583..1548)
                     /locus_tag="QQK36_RS24160"
                     /old_locus_tag="VEV11M_46880"
     CDS             complement(583..1548)
                     /locus_tag="QQK36_RS24160"
                     /old_locus_tag="VEV11M_46880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(924..1155)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ggcatcatgc ttttaacgga ctttaaaaaa cggtgaacgg atgcggattt taatccgtgt
       61 ctgtgaggcg ttgagcaacg agcaaagcga ggcggtagaa agagagccag cggcaggctc
      121 aggacgataa tccggggaaa atgtaccact cgcagagtgt taacatttga catcggattg
      181 tctatcctgc attggtgtcg agttggcaag gctgaggata atgctgagga taatgcagga
      241 tagacagagc actagagatt gcatgcaatc tcgtacctat cacctcagtc tgccgctggc
      301 tcttttccag aactaagaat gcgttcttaa aaatccaaac ttgataccgt ttaaatctat
      361 tttcacaaaa agagttttta ccctctcgtt tgttttctga atatataaaa ttaattcatc
      421 ctcaaaacct tcgtgcggat gaaaaccaaa tgtgccaaaa cttaatccat caataagcaa
      481 aataaggttt ttaaacctgt caggaatcac cgcttcactt cttaaaaaaa ataaaccaat
      541 agtcttattt ccattcaggt attcaatatt aatattcata cgttaatccg atttatcctt
      601 ctcaggggcg cgtttgtaac gccgtttacc tgaatcccag acaaacgccg tccgcttccc
      661 gtcatcagtg ccatccccta catcgtcggc aatgacaaga tcctcattgg tttctcgatc
      721 caactggagg acgtttttta gcgccccacc ggtcgagata aaacggcgct tgtgaagctg
      781 ccgcgtcagc tcaagaaacc actcaggatc gtttgccata tcttccggtt taacggagta
      841 tttcagcgtt tcagcaaccg cgctttgcag ttgctcggca acgttggcca caacctcacc
      901 tgtcttagtt tttactgccc gaatatcgat attcggctca tagttcaccc gcaagcaatc
      961 gcgccagagt tctacccaac gttcgtgctt aacgtagttc ttccctttaa accaagaagg
     1021 ttgcaccatc agcagacagt gaaaatgcgg atgtgcgctg ccatccttac ctcgcgtcac
     1081 ctccgtagcc ctgatccacc cctgaacagg tgatagctcc tttcgctttt ccattcgctt
     1141 aaacgccgca ttcattgctg taaggactgt tccaagttca cctatctcgc agttcctgac
     1201 agttaacgtc agaaacaacc atcgggaaga cgggtaatcc acaacgattt tcggtagagc
     1261 ctgataaaaa cgggcttgcc acatgagggt tcttctccac tggcagacag ggcaatgacg
     1321 gacacggcaa aattgcgcac tacgaagccg taggcgcgtt tctccggttt ccatgatggt
     1381 agaccaacca aaacgtaaca aaccgctaca ggaggccata cggagtgcat agcgctcgaa
     1441 ttcagcagca gtctggtaga tacccccgac agaatcggta agcgtgcgct gggtatccca
     1501 aactgcatca cgggggctgt agtccgaaag gaatttatct tctgacatta agcctgctcc
     1561 gatctgggca ggctgtgatt tgatgtggta tattcatcac gtacctgtgc attgcgagcc
     1621 agtacatgtc accagagttt gccgctgagg tgacacattc gaaatgttgg ggagtgttga
     1681 cgcactcccc aacgccgatt aaaaacgcct caattctgct ccattttctt tgacctgtca
     1741 aaacccactc aaagcctcgt cattactggg ctaaaccact atttcatatc cgccatttat
     1801 cccttaagta taaatacaag gatcacgcgc gagaattgcg caaaaaataa ccacaatttt
     1861 aaccccccaa aacaagcacc cccgaacggc ttaccccaac cttttccaca cacccgatca
     1921 ccaaaacagg gaataaaaac aaaaccctgt cttacaggtg gatggcctca cttcgtgaaa
     1981 ccatccaccc cgcataagtt ttttaatcgg tggtgtcgtc aatcactaaa tcggaacccc
     2041 aaaggggacc cccacgattt agcatttgac gagattaact ctcggagat
//
