LOCUS       NZ_AP027977             2089 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain VE-V10-F plasmid pVE-V10-F_6, complete
            sequence.
ACCESSION   NZ_AP027977
VERSION     NZ_AP027977.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00587240
            Assembly: GCF_030162175.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Whole genome sequencing of colistin-resistant Escherichia coli
            isolated from Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 50x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 18:33:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,125
            CDSs (total)                      :: 5,005
            Genes (coding)                    :: 4,718
            CDSs (with protein)               :: 4,718
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 287
            CDSs (without protein)            :: 287
            Pseudo Genes (ambiguous residues) :: 0 of 287
            Pseudo Genes (frameshifted)       :: 108 of 287
            Pseudo Genes (incomplete)         :: 182 of 287
            Pseudo Genes (internal stop)      :: 47 of 287
            Pseudo Genes (multiple problems)  :: 49 of 287
            Pseudo Genes (short protein)      :: 4 of 287
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027977.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="VE-V10-F"
                     /db_xref="taxon:562"
                     /plasmid="pVE-V10-F_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.95712843 N 105.82005564 E"
                     /collection_date="2020-07-03"
     gene            complement(30..296)
                     /locus_tag="QQK73_RS25620"
                     /old_locus_tag="VEV10F_49150"
     CDS             complement(30..296)
                     /locus_tag="QQK73_RS25620"
                     /old_locus_tag="VEV10F_49150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(300..1265)
                     /locus_tag="QQK73_RS25625"
                     /old_locus_tag="VEV10F_49160"
     CDS             complement(300..1265)
                     /locus_tag="QQK73_RS25625"
                     /old_locus_tag="VEV10F_49160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(641..872)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ctcagtctgc cgctggctct tttccagaac taagaatgcg ttcttaaaaa tccaaacttg
       61 ataccgttta aatctatttt cacaaaaaga gtttttaccc tctcgtttgt tttctgaata
      121 tataaaatta attcatcctc aaaaccttcg tgcggatgaa aaccaaatgt gccaaaactt
      181 aatccatcaa taagcaaaat aaggttttta aacctgtcag gaatcaccgc ttcacttctt
      241 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
      301 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      361 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      421 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      481 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      541 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      601 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      661 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      721 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      781 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      841 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      901 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      961 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
     1021 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
     1081 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
     1141 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
     1201 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1261 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1321 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1381 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1441 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1501 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1561 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1621 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1681 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1741 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1801 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1861 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1921 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1981 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     2041 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcac
//
