LOCUS       NZ_AP027970             2043 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain VE-T9 plasmid pVE-T9_7, complete sequence.
ACCESSION   NZ_AP027970
VERSION     NZ_AP027970.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00587239
            Assembly: GCF_030162155.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Whole genome sequencing of colistin-resistant Escherichia coli
            isolated from Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 581x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 18:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,719
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,363
            CDSs (with protein)               :: 4,363
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 87 of 241
            Pseudo Genes (incomplete)         :: 150 of 241
            Pseudo Genes (internal stop)      :: 44 of 241
            Pseudo Genes (multiple problems)  :: 38 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027970.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="VE-T9"
                     /db_xref="taxon:562"
                     /plasmid="pVE-T9_7"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.96321052 N 105.81806181 E"
                     /collection_date="2020-07-03"
     gene            join(1806..2043,1..731)
                     /gene="mobV"
                     /locus_tag="QQK52_RS23590"
                     /old_locus_tag="VET9_45390"
     CDS             join(1806..2043,1..731)
                     /gene="mobV"
                     /locus_tag="QQK52_RS23590"
                     /old_locus_tag="VET9_45390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(793..1227)
                     /locus_tag="QQK52_RS23595"
                     /old_locus_tag="VET9_45400"
     CDS             complement(793..1227)
                     /locus_tag="QQK52_RS23595"
                     /old_locus_tag="VET9_45400"
                     /inference="COORDINATES: protein motif:HMM:NF017542.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          4..728
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.694"
                     /coverage="96.0"
                     /evalue="2.47e-145"
ORIGIN
        1 gccctgagtg gtggaaagag gccacaccac ggcagcaggc ggagtttttc gcccgctctg
       61 agcaatggct ggagaagaaa tacgggaagg atcgtgttgt ggcggcggtg gttcaccggg
      121 acgaggccac cccgcacctg tctgcgttcg tggtgccatt gacgcaggat ggacggctgt
      181 ccgcgaaaga gtttattggc gggcggtcaa agatgcgtga agatcagagc acctacgctg
      241 aatcagtaaa aaaactgggt cttgagcgcg gcatcgaggg aagccgcgcc acgcatcaga
      301 cagttcagca ctactacgaa tcgataaacc gtggcacccg tagccaggta tcgatctcgc
      361 cggaggcgct ggagccgcgt gtattgcgga agggcatttt caccaaagac gtggaagatc
      421 aggcagccat agccaaacgt ctaagccacg cagtgaacga tggctttgca ggaaccatcg
      481 ctatggcctc tcagagcgcc cagaacgcaa aaagagcgcg tgagctacag aagactatgg
      541 atgcgcagca gaaacgccta cagagcgtca cagagccttt taaggggctt tccagagagc
      601 agatgaccga aatcctgatg atggctcagc ggtttaagca gcaaaaccag gaaaaggaaa
      661 aacagcagcg tattgaacgt gaaaaacagc gacagacgcg atcgcgtggc atgggtggta
      721 tggagcgtta gttttgatgc gatgcaggat agggctaacg ccgcaaagtg acgtcatttg
      781 cggcattcca gattaggttc tgccgccctg tataacgctg aggccgtttt ttagtgcttt
      841 cttgtggttg ctgacagtac ctttccagcc aaactgaggg ttcagacggt aactgtaact
      901 gcgccctact tttgggcctt gaagaatcac ggataggtca attagcgcct taaccgaacg
      961 gcttacattg gttttctgca tacctaaagc gtcggcaata tcggcttggg caatttggat
     1021 gtaattttca taatcgagat cagctaacag catcatcaag actttgaatt gctcccctgt
     1081 gagggtctta gcaaggatct ttaaagcgtc ttggttcata gtgaaatgcc tctcaaaagc
     1141 tgacttctgt ttgggtcgga taactgcgac aaaactacct acctcttccc ctgtagcagt
     1201 gtctatctga gtaattcggt tactcattta agaggctctc ccaaaaaaat agttatcaat
     1261 aatgataaca acgttatcaa taatgataac tatcgtcaag cgagagccgc gccccacaag
     1321 ggctgagaga aggctctctt ctgtatctta tagcgagaag ctcatatgcc aggatagcga
     1381 accctatttt catcggttcg tgcgtaagca tttctatgcc cgttcaggtg atattccgct
     1441 tcctcgttca ggctgttcgt tcctcgctgc gctgcggtac gcctcagcct gacctgcggc
     1501 gagcggcgcg tgctctttca aagacgactt tgttgcctga cattcgtcaa gggcgagcgg
     1561 agcgagtgca tttcaccctt gacgacgcac aaataaaaca ggcttggcag tggtgggagg
     1621 tgacgcggag cgacgccttc cacctgccag accgccgttg attttgacct tctggcagct
     1681 tgctgcccga acgattttgc gtagcaaagt ctagtgagta cactatcatc ttgatagtgg
     1741 cccgccggag gtatcgcaat gcgctccccc gcctggctga atgaatgccg gaatcagggg
     1801 ggaacatggc gaactacgcg ataatgcgct gcaaaaagct gacgggaatg ggaagcgtgg
     1861 ccagcgcgtt gcagcactgc taccgagagc gtgagacgcc gaacgctaac gctgaacgga
     1921 caccagaaaa ctactgctct gtgtccaaat caacggatca ggcaatgggg cgtgtgcggg
     1981 agctgctgcc ggaaaaacgc cgtaaagatg cggtactggc cgttgaatac gttatgacag
     2041 caa
//
