LOCUS       NZ_AP027969             2059 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain VE-T9 plasmid pVE-T9_6, complete sequence.
ACCESSION   NZ_AP027969
VERSION     NZ_AP027969.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00587239
            Assembly: GCF_030162155.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Whole genome sequencing of colistin-resistant Escherichia coli
            isolated from Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 581x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 18:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,719
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,363
            CDSs (with protein)               :: 4,363
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 87 of 241
            Pseudo Genes (incomplete)         :: 150 of 241
            Pseudo Genes (internal stop)      :: 44 of 241
            Pseudo Genes (multiple problems)  :: 38 of 241
            Pseudo Genes (short protein)      :: 4 of 241
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027969.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="VE-T9"
                     /db_xref="taxon:562"
                     /plasmid="pVE-T9_6"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.96321052 N 105.81806181 E"
                     /collection_date="2020-07-03"
     gene            26..556
                     /locus_tag="QQK52_RS23580"
                     /old_locus_tag="VET9_45370"
     CDS             26..556
                     /locus_tag="QQK52_RS23580"
                     /old_locus_tag="VET9_45370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000012591.1"
                     /translation="MSGFIPFVGSKYENSIYGLKLLVLGLSHYGDADDNYPDFTKDVIA
                     ENAYTTGNRFFTILTNVLRLSRESITDDQRKITWENIAFYNYIQQIVGEKGRISPTKEM
                     WDSAREPFLSVIKQLQPNVILVLGTELWDHLPEVDGVEWCWIRHPSSSNFSYDQAFNNL
                     KKSIGMENIVYLP"
                     /name="hypothetical protein"
     gene            1092..1397
                     /locus_tag="QQK52_RS23585"
                     /old_locus_tag="VET9_45380"
     CDS             1092..1397
                     /locus_tag="QQK52_RS23585"
                     /old_locus_tag="VET9_45380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_284345382.1"
                     /translation="MLSKKQTEKPETVEEMLKRLGFKSLKETLQKPIFLINFGAVKNMQ
                     SVVDEAFDEITKAKQGWYWQKVVDCLNENTNANVKLESIKTMYKRAKKKREDEESE"
                     /name="hypothetical protein"
ORIGIN
        1 atcaccccga agaaaggagt atgccatgag tggtttcata ccatttgtcg gtagcaagta
       61 tgagaacagt atctatgggt taaaattact ggtcttaggt ttgtcgcatt atggtgatgc
      121 tgatgataac tatccagatt ttactaagga tgttattgca gagaatgcct atacaacagg
      181 taaccgattc ttcacgatac tgactaatgt acttcgattg tctagggaaa gcataactga
      241 tgatcaaagg aagataactt gggaaaacat agcgttctac aactacatac aacaaattgt
      301 tggagaaaaa ggtcgaatat ctcccactaa agaaatgtgg gatagtgccc gagagccatt
      361 cctaagtgta attaagcaac tccaaccaaa tgtgatcctc gttctaggta ctgaactttg
      421 ggatcatctt cctgaagttg atggtgttga atggtgttgg attagacatc caagctctag
      481 taatttctca tatgatcaag catttaataa cctcaaaaaa tcaataggca tggaaaatat
      541 cgtttattta ccataacgtc gcattacgcg caccgctgaa acggcctgtg cgcgattttc
      601 ccgtctgagc ctcaaaacaa cacaccttcc cccccaaaaa ccgctcagaa tagcttctgt
      661 gagttttacg cgcatgaatc gttatgcact caggtgcatg gcaaccgatt agcgacccga
      721 ttctctgcct ggtcgttgaa acccgatgaa aagtccctcc cgaaaacggt gaacgggcgg
      781 ataaattttt tatccggctg taaagcgttg gacaagcgca gcgcgtcaga aaaaagactc
      841 gtagaggctt cggggtatca ggggtatccc ctgagcacgc gactgaagat aaacggaaaa
      901 aatgtcccca ttttgtcccc gtttgtctac agtcgctgtc gtgccttaaa aaataaagcc
      961 gcaacacaat atcgccactt tatcaccact ttatcaccat caaaaatggg gtatcgccac
     1021 tttgtcacca ctttgtcacc actttatcgc tacaaaatcg tctgttatgc cttaattttg
     1081 ggagatagct aatgttgagc aaaaaacaaa ctgaaaaacc agaaacggtc gaggaaatgc
     1141 ttaagcggtt aggtttcaaa agtctgaagg agacacttca gaaacccatt ttccttatta
     1201 actttggggc ggtcaaaaac atgcagtccg tcgtggatga agcctttgat gaaatcacaa
     1261 aggcgaagca gggatggtac tggcaaaagg tcgtagactg tctgaatgag aatacgaacg
     1321 ccaacgtaaa gctcgagtcc attaaaacca tgtacaaacg ggcaaagaaa aaacgggaag
     1381 atgaggagtc agagtgaccg tgcacacgca agcatccgat gaaatgacgc ttcctcgctc
     1441 attcgctcac ttcgttcggt catgagactg cggtgagcgt tgctatttta acattatgat
     1501 ttgaagggag ttcatactgt aacctgcgac tatgaagtcg taagttacta atagcgatgc
     1561 ctttagttgt aatttttata tctctaaatg tacaaaagcc ccgtgacaga atcacagggc
     1621 ttcggtactc taattcgtgc agatacctgc tggattagac gtcgcaatca aatcttagca
     1681 gggctggcta gccatgtcac taccagctta ataccattca acgacattaa aatcatatct
     1741 gcaccttaga cgctcaggtc tacgccccgg actccggcag gataaacagg caaattggtg
     1801 ggaacgtaac ttaaccgcga aagagttagg ctctaccccg tctcgctgta cgataacaag
     1861 cacctgataa aatgagcgaa taaggcagca gtaagcggga aagtgtgatt gcgagaagtc
     1921 tgacacgaac cccacacaaa tcgaatccgt tgcaagatga gacacacaag aaaagagctg
     1981 gacgaaggtc gctccgctcc ccgtggtttt ttatataccc accacggggg cattaggttt
     2041 ttatgttcaa aaaacaacc
//
