LOCUS       NZ_AP027956            13190 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain VE-F5 plasmid pVE-F5_3, complete sequence.
ACCESSION   NZ_AP027956
VERSION     NZ_AP027956.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00587232
            Assembly: GCF_030162015.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Whole genome sequencing of colistin-resistant Escherichia coli
            isolated from Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13190)
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 90x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 17:58:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,840
            CDSs (total)                      :: 4,719
            Genes (coding)                    :: 4,521
            CDSs (with protein)               :: 4,521
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 64 of 198
            Pseudo Genes (incomplete)         :: 122 of 198
            Pseudo Genes (internal stop)      :: 50 of 198
            Pseudo Genes (multiple problems)  :: 37 of 198
            Pseudo Genes (short protein)      :: 4 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027956.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="VE-F5"
                     /db_xref="taxon:562"
                     /plasmid="pVE-F5_3"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.95712843 N 105.82005564 E"
                     /collection_date="2020-07-03"
     gene            join(13008..13190,1..264)
                     /locus_tag="QQJ99_RS24120"
                     /pseudo=""
     CDS             join(13008..13190,1..264)
                     /locus_tag="QQJ99_RS24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            401..757
                     /gene="ddp1"
                     /locus_tag="QQJ99_RS24125"
                     /old_locus_tag="VEF5_46390"
     CDS             401..757
                     /gene="ddp1"
                     /locus_tag="QQJ99_RS24125"
                     /old_locus_tag="VEF5_46390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000456533.1"
                     /translation="MEYQVREFINEKYTKAVNILKDNLKENYHVFYGVRLIEILFPASE
                     YGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISFDKILDASLLEGTNIVVLECITLA
                     DLLTNDNIDFLYKS"
                     /name="ddp1"
     gene            complement(754..1731)
                     /locus_tag="QQJ99_RS24130"
                     /old_locus_tag="VEF5_46400"
     CDS             complement(754..1731)
                     /locus_tag="QQJ99_RS24130"
                     /old_locus_tag="VEF5_46400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="WP_024245156.1"
                     /translation="MRYLTELICDLYRLTDHHKLTTATFKNLADIKLFKPTADADTILQ
                     LENIFSEYSLRYIDKDLAEILSNLITTDKKHSLDFDFNKIQSLTDSKSFGCGWSKVING
                     SWFKDLYSWGEGMYPAENLKAENILDWKDNIWHIEHEGFPPRPPINVKYYSWFDRYVAS
                     NSGGSHHAAMVVYQSVRDGLEYKREAVIKQLSIDPGTVEILDKNYYSFIFQIKTLNKET
                     RIYTSEYEFTDALKDFGQNRHTILKPVNYVSNIKLVFIPKDALKTNSETFRNWFHSAIS
                     CGKIISLPDYLKNPAHYHTLHYSHEVDSITLGDPSRKFKLKGDS"
                     /name="DUF6685 family protein"
     gene            complement(1756..2037)
                     /locus_tag="QQJ99_RS24135"
                     /old_locus_tag="VEF5_46410"
     CDS             complement(1756..2037)
                     /locus_tag="QQJ99_RS24135"
                     /old_locus_tag="VEF5_46410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185482.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="hypothetical protein"
     gene            complement(2135..2797)
                     /locus_tag="QQJ99_RS24140"
                     /old_locus_tag="VEF5_46420"
     CDS             complement(2135..2797)
                     /locus_tag="QQJ99_RS24140"
                     /old_locus_tag="VEF5_46420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            3178..3822
                     /locus_tag="QQJ99_RS24145"
                     /old_locus_tag="VEF5_46430"
     CDS             3178..3822
                     /locus_tag="QQJ99_RS24145"
                     /old_locus_tag="VEF5_46430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203396.1"
                     /translation="MTSNVPTSFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGTRLERPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKAKKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISNALKRKTGQKSSK"
                     /name="recombinase family protein"
     gene            complement(4110..>5042)
                     /locus_tag="QQJ99_RS24150"
                     /old_locus_tag="VEF5_46440"
                     /pseudo=""
     CDS             complement(4110..>5042)
                     /locus_tag="QQJ99_RS24150"
                     /old_locus_tag="VEF5_46440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /name="methyl-accepting chemotaxis protein"
     gene            complement(5094..5798)
                     /locus_tag="QQJ99_RS24155"
                     /old_locus_tag="VEF5_46450"
     CDS             complement(5094..5798)
                     /locus_tag="QQJ99_RS24155"
                     /old_locus_tag="VEF5_46450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<5863..6804)
                     /gene="intI1"
                     /locus_tag="QQJ99_RS24160"
                     /old_locus_tag="VEF5_46460"
                     /pseudo=""
     CDS             complement(<5863..6804)
                     /gene="intI1"
                     /locus_tag="QQJ99_RS24160"
                     /old_locus_tag="VEF5_46460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /name="intI1"
     gene            6953..7791
                     /gene="aadA1"
                     /locus_tag="QQJ99_RS24165"
                     /old_locus_tag="VEF5_46470"
                     /pseudo=""
     ARG             6954..7741
                     /name="aadA1"
                     /gene="aadA1"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7912..8609)
                     /locus_tag="QQJ99_RS24170"
     CDS             complement(join(7912..8360,8360..8609))
                     /locus_tag="QQJ99_RS24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_225528490.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGIGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            <8665..8916
                     /gene="tnpA"
                     /locus_tag="QQJ99_RS24175"
                     /pseudo=""
     CDS             <8665..8916
                     /gene="tnpA"
                     /locus_tag="QQJ99_RS24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525033.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /name="tnpA"
     gene            complement(9108..9302)
                     /locus_tag="QQJ99_RS24180"
                     /old_locus_tag="VEF5_46500"
     CDS             complement(9108..9302)
                     /locus_tag="QQJ99_RS24180"
                     /old_locus_tag="VEF5_46500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053273080.1"
                     /translation="MEKIGNDIPHLIDQAIDFMASTQAFREYLKKLPPRNTIPSDIPEE
                     NVPLYLQRLEYYRQLYRPK"
                     /name="hypothetical protein"
     gene            complement(9604..10301)
                     /locus_tag="QQJ99_RS24185"
     CDS             complement(join(9604..10052,10052..10301))
                     /locus_tag="QQJ99_RS24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            complement(10376..10657)
                     /locus_tag="QQJ99_RS24190"
                     /old_locus_tag="VEF5_46530"
     CDS             complement(10376..10657)
                     /locus_tag="QQJ99_RS24190"
                     /old_locus_tag="VEF5_46530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10647..10898)
                     /locus_tag="QQJ99_RS24195"
                     /old_locus_tag="VEF5_46540"
     CDS             complement(10647..10898)
                     /locus_tag="QQJ99_RS24195"
                     /old_locus_tag="VEF5_46540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            12132..12968
                     /locus_tag="QQJ99_RS24200"
                     /old_locus_tag="VEF5_46550"
     CDS             12132..12968
                     /locus_tag="QQJ99_RS24200"
                     /old_locus_tag="VEF5_46550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_001050931.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDIRIPYGFRG"
                     /name="RepB family plasmid replication initiator protein"
     ARG             6840..7744
                     /name="aadA"
                     /gene="aadA"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.62"
                     /coverage="114.45"
     Typing          2..191
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.906"
                     /coverage="100.0"
                     /evalue="3.6e-88"
     Typing          13009..13190
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.814"
                     /coverage="100.0"
                     /evalue="1.22e-83"
ORIGIN
        1 gtgttagcca ttcttggtgc gtttaaatgg gctgtattct ggctgggtgg tattttgtgt
       61 gtcatcactt ggtatcatct cgatcaccag ttatggacgc cacaaaatct gactataccg
      121 gtgattacgg caacctggat tttgggactg ctggctgagc ctctgatgga gctgctcaac
      181 aaaactatgc cttggcacaa gttacttgtt cccgaacctg cggacgaaag caccgagaac
      241 agtagcgaga taagccagca atgatgctac tcagcagtta tgccagtccc tgccttttca
      301 ctgcgatgca ggatagggca aacgccgcaa aatgacgtct ctgacgccat tctgcaacgt
      361 ttgcccggtg acctacagtc ggttcttgtc ggagaatttt atggaatatc aggtgagaga
      421 gtttattaac gaaaaatata ctaaggctgt taatatttta aaggataacc ttaaagaaaa
      481 ctaccatgtt ttttatggtg tgagattaat tgagattctt tttccagcca gtgagtatgg
      541 cactgatgct ttctttaaag agtttgaatt aattaacagt gtaatacttc cattggtaat
      601 ctttgattta acacaacgca agccaatgat gattatcagc ttcgataaga tacttgatgc
      661 cagtttgttg gaaggaacaa atattgtggt tcttgaatgt attaccctcg ctgatcttct
      721 gacaaatgat aacattgatt ttttatataa aagctaagaa tctcctttca gtttaaattt
      781 tctgcttggg tcgccaagtg ttattgagtc tacttcatgt gagtaatgaa gtgtatgata
      841 atgtgctgga tttttcagat aatcagggag tgatattatt tttccgcagc taattgcaga
      901 atgaaaccag ttgcggaatg tctcgctatt tgtttttaat gcatcttttg gaataaatac
      961 taattttatg ttggaaacgt aattaacagg tttaagtatg gtatgtctgt tttgtccaaa
     1021 gtcttttaaa gcatctgtaa actcgtattc agatgtataa attctagttt ctttattaag
     1081 ggttttgatc tggaatatga atgaatagta attcttatca agtatttcaa cagtgcctgg
     1141 gtctatactc agttgtttaa taacagcttc tcttttatac tctaacccgt ctctgactga
     1201 ttgatataca accattgctg catgatgtga gccaccactg tttgaagcta catatcggtc
     1261 aaaccaagaa taatatttaa cattaatagg ggggcgtggg gggaaacctt catgctcaat
     1321 gtgccatata ttatctttcc agtctaaaat attttcagct ttcagatttt cggcaggata
     1381 catcccctct ccccaagaat aaagatcttt gaaccaacta ccatttatta ccttgctcca
     1441 tccacaaccg aaacttttcg agtcagtcag agattgtatt ttgttaaaat cgaagtccag
     1501 agagtgtttc ttatctgtag ttattaagtt gctcagaatt tcagccagat ccttgtcaat
     1561 atatctcagt gagtattcgc taaatatatt ttcaagctgc aatatggtgt ctgcatctgc
     1621 tgtaggttta aataacttaa tatcagcaag atttttaaac gttgctgtgg tcagtttatg
     1681 gtgatctgtc aacctgtaga ggtcacagat caattcggtt aaatatctca tagattttca
     1741 tccatgcttc tatggttaga attttgcaga ctcaagcaac cagtgattct ttatgttttc
     1801 atcctggctg ttgtacatcg ccagcgccgc tttgagaacg gttgttttgc tctgccctgt
     1861 tctgatagct tcagcttcca gtatgtcctc aaaatgggct gaaagtctga agttcatcaa
     1921 ctttggttta cccactacag gcgctctggt agcgccggag ataaaacgcg cggtttctgc
     1981 gtttgttgct ggttgctgag attcatcgat gttcggacgg tttagtttta aagccatgac
     2041 aatttcccct tgtacggtat gcattaatag taaatatcat actatcttta tattatcttt
     2101 acaatgtaaa gatttataga tggtattgat attactataa aattccaatt tcctgcgcca
     2161 ctagttcaat ctgtgctttc gctgttgaaa ggcttgatgc ccggctaacg tcatgtaccc
     2221 ctgccccttc attacaggcg ctttcataca catccagctc ggatatccgg gttttaagag
     2281 gttgaatcca gatgttgtcc gaacgcagta atttatccag atcaatggcc gcttttctgt
     2341 ggcggggttt cgctgagttg attctggtga acagcaccca cggttccagg gctgcgttag
     2401 ctttctgagc ggtgcggatt ttctctgtaa cactggtcag agtttcgctt tcgaaatcag
     2461 aggacggctt aaccagtgtt accacgatat ctgatactgt cagggcgcta cggaactcct
     2521 ggctgtcgtg gccggggcaa tcgacaatga ggacttcaca cagcttacta agtcgcttaa
     2581 tttcattgct gacgtttccg taagctgcgt gaaccgggat agtgagtaag ccgttatctg
     2641 tccgcttttc gttccagctc aacagatcgt ggtttttatc tgtcttgagg ataattaccg
     2701 acttaccttt attgaccagc attgagcctg tgttagccac gtaggtgctt ttgcctacac
     2761 cacccttatc tgatacgaca agcaagattt tacccatttc agaattcctc tctttatgac
     2821 gtaaagatta tgtaaagact gtcttatctt tacattgtgt gttgttcgta aagattattt
     2881 tgtctttaca gtgtctttat attacacatg atttaccatg taaagcacaa ataaagataa
     2941 gcttgtattt atgttatctt tattgtgtaa agatttgtgt gtatttatag tggctttacg
     3001 tggtaaatct gagaccgtct ttacatcatc tttatgttgt aaatttctgt gtgacattgt
     3061 cactttatgg tcggaggtct gttttctggt tctttttgag tgtgcctttc ctgtcgatct
     3121 actttctggt gcattttggg tatagtggtg taaccttatg tgaaccggtt ctgtaatatg
     3181 acttcaaatg taccaacaag ctttgtccgc gcttatctga gagcttcaac agcagaacag
     3241 gatgcttcgc gtgccctgga aactatcgag gcttttgcca gggaaagagg cctgatcatt
     3301 tgcagctact atattgagaa tgaatccggc acccgtcttg agcgcccgga actttttcgt
     3361 cttctgagag attgccaaca aaatgacatt ttgcttatcg aggatgtgga cagactttcc
     3421 cgcctgtcag gggaagactg gaatacgttg aagaaaatga tccggcagaa agacatccgg
     3481 gttatggcgg tgaatgtgcc gacgacatgg attaattctg gtatgagtga gtttgacagc
     3541 cggttgtttg cagccattaa cgatatgctg ctggatatgc tggccgcagt tgccagaagg
     3601 gactatgaac aacgtcggga acgccagaaa caagggatag agaaagcaaa gaaagacggc
     3661 aaatataaag ggcgtaaacc taatcaagcc agacatgatg caattatcag gttgatagaa
     3721 agcggtagct cctggacaca ggtacagaaa gtgctagggt gcagccgtgg cacaatcagt
     3781 aatgcgctaa aacgtaaaac cgggcagaaa tccagtaagt agaggagtag ggggctaata
     3841 tacctgcaag gcaggatatt aactcaggag attgggtgtg tggtgtttag tcaatgcatt
     3901 tgaatattgt aactaaaata gggggaaccg cagaattcgg aaaaaatcgt acgctaagct
     3961 aacggtgttc tcgtgacagc tctttgacta ggctttctaa ggccattctg atagccctga
     4021 cttcctgaaa agccatggct aaaatttgtg cggctaaaag ggataaccga tggtaaagta
     4081 agttatccct gtcgagatac tgaaaagcgt tatcctcgtt tttcccaaaa ctgttttgcc
     4141 agttcgctca gagcgctagt taactgagcg acagatttcg cactttgcaa attattctgc
     4201 ccggtctgta cattggtatc agcagcatcg cgtatattga taatactgcg gtttatgtct
     4261 tcacttactg ctccctgctg ctcgaccgca gtcgctattt gcgcgttcat gtcggtaatt
     4321 tcgttaacgc gttggccaat tccatcaaga gctgtagctg cttcctctgc gtgagctaca
     4381 ctcgtgtgcg cttgccgact actttgctcc atgactgtaa cagcggattg cgctcgctct
     4441 tgtagagcgc tgatcatgct ttgaatatcc gttgtcgatt gctgtgtgcg agcagcaaga
     4501 ctgcgaacct catcggcgac aacagcaaaa ccacgcccct gctcaccagc acgcgcggcc
     4561 tcaattgctg cgttgagtgc caacaaattc gtttgctcgg cgatccctcg tataacgtca
     4621 agaacttttg atatctcgtt actttgacct tcaagctcat gaataacctg agtggcttgc
     4681 ctaatttcac cttcaagggc agtgattgac tggcttgtgt gggctaccag acgctggcca
     4741 gatgccgtct cagtgtctgc tcttccggcc gcatctgcag catgctgtgc attgctcgca
     4801 acctcttgaa tgcttgccac catttggttt actgccgttg ctatttgatc tgtctctgcc
     4861 tgctgctcaa ctgtaagtac attgcttgac tcaatatcct ttagtaggcc tcgggtgtgt
     4921 tcgctaagcc gatttgatgc atcacctatg cgacctacta tggcgcctgt ttcagcttgc
     4981 atcattcgta aagcaaactc tatttggcca aactcatcgg tgcgcccagt gtagagggat
     5041 tgtcactgtt gcaaagttag cgatgaggca gccttttgtc ttattcaaag gccttacatt
     5101 tcaaaaactc tgcttaccag gcgcatttcg cccaggggat caccataata aaatgctgag
     5161 gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc gtaagccgtc
     5221 ttcatggatt taaatcccag cgtggcgccg attatccgtt tcagtttgcc atgatcgcat
     5281 tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg gcaccggcct
     5341 tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt gttgatgaat
     5401 cgcgggatct gccacttctt cacgttgttg aggattttac ccagaaaccg gtatgcagct
     5461 ttgctgttac gacgggagga gagataaaaa tcgacagtgc ggccccggct gtcgacggcc
     5521 cggtacagat acgcccagcg gccattgacc ttcacgtagg tttcatccat gtgccacggg
     5581 caaagatcgg aagggttacg ccagtaccag cgcagccgtt tttccatttc aggcgcataa
     5641 cgctgaaccc agcggtaaat cgtggagtga tcgacattca ctccgcgttc agccagcatc
     5701 tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg
     5761 atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg
     5821 ggatgataag tttatcacca ccgactattt gcaacagtgc ccatgcgtgt aaatcatcgt
     5881 cgtagagacg tcggaatggc cgagcagatc ctgcacggtt cgaatgtcgt aaccgctgcg
     5941 gagcaaggcc gtcgcgaacg agtggcggag ggtgtgcggt gtggcgggct tcgtgatgcc
     6001 tgcttgttct acggcacgtt tgaaggcgcg ctgaaaggtc tggtcataca tgtgatggcg
     6061 acgcacgaca ccgctccgtg gatcggtcga atgcgtgtgc tgcgcaaaaa cccagaacca
     6121 cggccaggaa tgcccggcgc gcggatactt ccgctcaagg gcgtcgggaa gcgcaacgcc
     6181 gctgcggccc tcggcctggt ccttcagcca ccatgcccgt gcacgcgaca gctgctcgcg
     6241 caggctgggt gccaagctct cgggtaacat caaggcccga tccttggagc ccttgccctc
     6301 ccgcacgatg atcgtgccgt gatcgaaatc cagatccttg acccgcagtt gcaaaccctc
     6361 actgatccgc atgcccgttc catacagaag ctgggcgaac aaacgatgct cgccttccag
     6421 aaaaccgagg atgcgaacca cttcatccgg ggtcagcacc accggcaagc gccgcgacgg
     6481 ccgaggtctt ccgatctcct gaagccaggg cagatccgtg cacagcacct tgccgtagaa
     6541 gaacagcaag gccgccaatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc
     6601 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc
     6661 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     6721 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     6781 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     6841 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     6901 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     6961 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     7021 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     7081 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     7141 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     7201 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     7261 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     7321 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     7381 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     7441 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     7501 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     7561 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     7621 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     7681 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg taatcggcaa
     7741 ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     7801 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     7861 atttttaagc gtgcataata agccctacac aaattgggta atgactccaa cttattgata
     7921 gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccaccg attttgagaa
     7981 cgacagcgac ttccgtccca gccgtgccag gtgctgcctc agattcaggt tatgccgctc
     8041 aattcgctgc gtatatcgct tgctgattac gtgcagcttt cccttcaggc gggattcata
     8101 cagcggccag ccatccgtca tccatatcac cacgtcaaag ggtgacagca ggctcataag
     8161 acgccccagc gtcgccatcg tgcgttcacc gaatacgtgc gcaacaaccg tcttccggag
     8221 cctgtcatac gcgtaaaaca gccagcgctg gcgcgattta gccccgacat agccccactg
     8281 ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc tgtatgcgcg aggttaccga
     8341 ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga ggccaatgcc cataatgcgg
     8401 gcggttgccc ggcatccaac gccattcatg gccatatcaa tgattttctg gtgcgtaccg
     8461 ggttgagaag cggtgtaagt gaactgcagt tgccatgttt tacggcagtg agagcagaga
     8521 tagcgctgat gtccggcggt gcttttgccg ttacgcacca ccccgtcagt agctgaacag
     8581 gagggacagc tgatagaaac agaagccact ggagcacctc aaaaacacca tcatacacta
     8641 aatcagtaag ttggcagcat caccgtgcat cttgtcgtaa tagtgccgcc aaaaatctca
     8701 atatctactc tgatggggca cctgaaaggt cgcagtgcaa ttaggctcta caatcgattc
     8761 ccgcatatca ggaagaagtt atggggaaac catttttggt cgcggggtta ttttgtcgat
     8821 acggtaggcg tgaacgaaga aattatcaga cgatatgtga ggcatcagga aaagatggaa
     8881 caaacacatg aacagcagat ggagttgcta gagtagaaaa cggaatatga catttgcccc
     8941 ccttataggg ggcattctga aaagccacct cctcaggagg tggtctttta ctctatcaac
     9001 aggctgtgtg atcaacaatg gtcctctctc tgacagttct gatttcaaat aatcagctta
     9061 aaaatacctt tcaaaagtaa acaatttcct ctgagggcac attttatcta ctttggcctg
     9121 taaagctggc gataatattc cagacgttgt agatataacg gcacgttttc ctcaggaata
     9181 tcggacggaa ttgtgttacg gggggggagt tttttcaaat actctcggaa cgcctgggtt
     9241 gatgccataa aatctatagc ttgatctata agatgcggaa tgtcattacc tattttttcc
     9301 atgataatat ctccgtatta cttcgcacca gcggttaatt ttagataaga tgctaaaagt
     9361 agcattaagg aaaacgtgct taatcctcaa tgttgactcc cggaaatctt ttaacttttt
     9421 aagtttctta atgaatcaat aaattacata gttcgtaagc aagtatatta ttgcctgatt
     9481 aaactatttt gatgctatca accatggaca aagcacctcg ttgcacataa cagagactat
     9541 gacgttattt ctgtcagagg ccatcacatg aaagaagtca aaaaaagggg taatgactcc
     9601 aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac
     9661 cgattttgag aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag
     9721 gttatgccgc tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag
     9781 gcgggattca tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag
     9841 caggctcata agacgcccca gcgtcgccat cgtgcgttca ccgaatacgt gcgcaacaac
     9901 cgtcttccgg agcctgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac
     9961 atagccccac tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg
    10021 cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg
    10081 cccataatgc gggcggttgc ccggcatcca acgccattca tggccatatc aatgattttc
    10141 tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca gttgccatgt tttacggcag
    10201 tgagagcaga gatagcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca
    10261 gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc tcaaaaacac
    10321 catcatacac taaatcagta agttggcagc atcaccaatc atttgatgat taatcttagc
    10381 gcaaatctgc gatctgatag acttcgctgc tttcccttct tccaacggca ataacgagta
    10441 aaacgacttt ctcatcaatg acttggtata caagtctgta gccagatgca cgaagtttga
    10501 ttttatagcg atcagcatga ccatgtaacc gggctgcggg tacgcgtgga ttttccagcc
    10561 gttctgccag tttctttttg aattgttcac ggatggtgtg gccgagtttc tgccattcct
    10621 tcagtgctct acggtcaaaa gccagttcat aggtcatcca ggttaacctt cacaatttcc
    10681 gggtttttca tgcgctcatc agcaacagcg tttaactcag catcttcagc gagttcccga
    10741 tagtaggcgt aaagttttgg tggaacgcaa tagaacgccg gttcgtttcg gttcaggatc
    10801 gcaacagcgt ccccttcacc ttcagctacg gttcccatag gatttttttt caggtcagta
    10861 atactggccg ctgtagttgt cagaatttga tagctcataa gccctccgct tgtctcattg
    10921 gcataatagc atctttaaaa gcactcttaa aagtgcttat gttgagaaaa acatcagaac
    10981 aggtgagata tggctctttc cgacatcgcc ttaaaacgct ctgtaacgcg ttctgagcgt
    11041 tttttcccgt ttcgttgtag tcagctacgg tttaacggtt tttaacgccg ctcaacgctt
    11101 tttagcggcc ttgttgctgt gatctgttgc catgatccgg ataggagttc aatcttgatc
    11161 aaaatttcct caaggcaacg actgttttgt aaataccctc taaaacgcgc tgtatcgcat
    11221 tctgagcgat tttttcgatt tcgcatggtt tcaccatggt tgtatcgaac gaagcggcag
    11281 aaagcgattc agggcttctc agtgaaagat ccaaaaagga tcaatcaacg gatcgtatga
    11341 gctgaactct tctggttttg agaaaggaga caccgctcgc cgcagccgaa cgaccgagtg
    11401 tagcgagtta gtgagcgagg aagcggaata gcacagggat tacattaggc acttacgcac
    11461 agtggttaaa aaatgagcat attgttttta tgtgtggttg tcttggttca gagtgatctg
    11521 tcctccgggt ttacgcaaag ttatccacag gcgctgtgaa ttttttgttt tgagcagagt
    11581 tatccacaga ttttttgtgt ttttttgctt attaaacttc ttgtttttaa gatcttaaat
    11641 gtagatcggt agatctactt taagatcttt aacttacttt aacttattct gcaaacattg
    11701 tgccagcttt tttatcaata atttcattaa gataggtgtt gttttttgtc gcctcaaaat
    11761 gaaaggtgga tgggtacgca ctgaaaggtg gatgggtacg cactgtaatt ttcatgaaag
    11821 gtggatggct gcgcactgaa aggtggatgg gtacgcactg aaaggtggat gggtacgcat
    11881 ttttctgttt ttattcagat attcgtatct ttgtatcttc atttctttag ttctttatag
    11941 tgctctaatt taacgtatga cgttaaaagg tggatacctg cgcatttcta tcatattgaa
    12001 tataaatgat aaaaattctt tcatgtgatc cgtgttttgg tggaaataca taaggtggat
    12061 aaaccacttt acatatttcc gcctttgtgg ttatgatttc accatcagaa cttaaaaacc
    12121 acaggtaatg tatgaactta ctgagcgtta caaagaaaac aaaaattcgc cacagaaatg
    12181 aaattaacag cacattctca tcgttgcctt tgtctgccag aagaattttg tttatggcta
    12241 tggcgcagat tgattcacgt gaagagttat ctgagggggc aacctttcgt attacggctc
    12301 gggaatatgc atttatcgct gatattgatg taacaggtgc ttaccggcag ttgaaagagg
    12361 gggcagaaga acttcaggcc agtgttattc gcattcctcg aaatcaactt ctcacacctc
    12421 agggcaatca aagctctgga catacgaaga agagaggtaa attaccttct gatgcagtca
    12481 ggcttatgaa cctgactgcg ttttgtgatt atgttgaaag tgaaggatat attgatattg
    12541 ctttttcaca tgtcattgag ccatatatca cgatgttaaa ggattcttac actactcagg
    12601 ttttgatatc gtctgtaaga ctcgctgatc aaaattcaaa tatgctttat cagtttatca
    12661 ggaaacaata cagcagtggt aaaaagacat ttttcgacat tgaagtagat gtattaaagg
    12721 atgaacttga attattcaga atagataatg gcgaaaaagt ttacctttat cagaatttta
    12781 aggattttaa taaggttttt ctgcaaaaaa atattgaaaa aataaatcaa tatacagaga
    12841 ttaatcatct cgaagttaaa atcgttgaga gagtcgcaag aagagcatca aagctaaggt
    12901 tttcttataa aatagataaa gaaagtgagg gcttagatat cagaatacca tacggtttca
    12961 gaggataatt gcataaactt cgttttgaga tcgaaatgag gtagctcatg gctaaaatct
    13021 atcaattccc tcagggggaa gaacgtgcta aattcaggaa agaaattgcc agggagcgta
    13081 agaaacgttt tgcagtgaaa acagggagca cttttgttaa gtggcttggc tgaggatggt
    13141 tttatcttcg tcttcttgtc gcgagtgttt tacattttat ctttgttttt
//
