LOCUS       NZ_AP027949             9902 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Escherichia coli strain VE-F10 plasmid pVE-F10_3, complete sequence.
ACCESSION   NZ_AP027949
VERSION     NZ_AP027949.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00587229
            Assembly: GCF_030161935.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Whole genome sequencing of colistin-resistant Escherichia coli
            isolated from Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   Suzuki,M., Vu,T.M.H. and Kasuga,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Contact:Masato Suzuki National Institute of
            Infectious Diseases, Antimicrobial Resistance Research Center; 4-2-1
            Aobacho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Coverage                   :: 232x
            Sequencing Technology             :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/23/2023 18:03:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,869
            CDSs (total)                      :: 4,747
            Genes (coding)                    :: 4,544
            CDSs (with protein)               :: 4,544
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 63 of 203
            Pseudo Genes (incomplete)         :: 134 of 203
            Pseudo Genes (internal stop)      :: 42 of 203
            Pseudo Genes (multiple problems)  :: 37 of 203
            Pseudo Genes (short protein)      :: 4 of 203
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027949.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="VE-F10"
                     /db_xref="taxon:562"
                     /plasmid="pVE-F10_3"
                     /geo_loc_name="Viet Nam:Hanoi"
                     /lat_lon="20.95712843 N 105.82005564 E"
                     /collection_date="2020-07-03"
     gene            898..1089
                     /locus_tag="QQK65_RS24250"
                     /old_locus_tag="VEF10_46510"
     CDS             898..1089
                     /locus_tag="QQK65_RS24250"
                     /old_locus_tag="VEF10_46510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2363..3060)
                     /locus_tag="QQK65_RS24255"
     CDS             complement(join(2363..2811,2811..3060))
                     /locus_tag="QQK65_RS24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            3173..4033
                     /locus_tag="QQK65_RS24260"
                     /old_locus_tag="VEF10_46540"
     CDS             3173..4033
                     /locus_tag="QQK65_RS24260"
                     /old_locus_tag="VEF10_46540"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            4408..4968
                     /locus_tag="QQK65_RS24265"
                     /old_locus_tag="VEF10_46550"
     CDS             4408..4968
                     /locus_tag="QQK65_RS24265"
                     /old_locus_tag="VEF10_46550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            4972..>5343
                     /locus_tag="QQK65_RS24270"
                     /pseudo=""
     CDS             4972..>5343
                     /locus_tag="QQK65_RS24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            5323..5568
                     /locus_tag="QQK65_RS24275"
     CDS             5323..5568
                     /locus_tag="QQK65_RS24275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041937891.1"
                     /translation="MSDFRALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(5600..6250)
                     /gene="tetR(A)"
                     /locus_tag="QQK65_RS24280"
                     /old_locus_tag="VEF10_46570"
     CDS             complement(5600..6250)
                     /gene="tetR(A)"
                     /locus_tag="QQK65_RS24280"
                     /old_locus_tag="VEF10_46570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_038989485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEARPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     ARG             6282..7555
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.81"
                     /coverage="100.0"
     ARG             6357..7552
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7587..8471)
                     /locus_tag="QQK65_RS24290"
                     /old_locus_tag="VEF10_46590"
     CDS             complement(7587..8471)
                     /locus_tag="QQK65_RS24290"
                     /old_locus_tag="VEF10_46590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(8609..9001)
                     /locus_tag="QQK65_RS24295"
     CDS             complement(8609..9001)
                     /locus_tag="QQK65_RS24295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <9005..9869
                     /locus_tag="QQK65_RS24300"
                     /pseudo=""
     CDS             <9005..9869
                     /locus_tag="QQK65_RS24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     ARG             3174..4030
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(404..1023)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.243"
                     /coverage="99.0"
                     /evalue="1.1799999999999999e-174"
ORIGIN
        1 tatgtgggcg tttatgtaac atatgtcgct gggccaactc ttctggtttc tgggttttta
       61 tcgtggatta caaggccgta atctgataat gcccctaatg ggtcagatct ctaggatagt
      121 agcaatacag caaggaactt cttgagatcc tttctgttgc gtcgtaatct catgtcctgc
      181 aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaatcaaga gctaccaact
      241 cttttttccg aaggtaactg gctttcacgg agcgcagata ccaaaactgt ccttctagtg
      301 tagccgcagt caggccacca cttcaagaac tctgtagaag cactctacta ctctgctccg
      361 tgaatcctgt taccagtggc tgctgccagt ggcgcatttg catgtctttc cgggttggac
      421 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
      481 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
      541 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
      601 gagggagcca ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
      661 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgc
      721 cccgctgggg cgtggccttt tcgcccttgt ccttagcttt cgttatgctt accgttacgt
      781 tatctgctta tcttccctcc gctcgccgca gactcatgac cgaacggagt gagcgaatga
      841 gcgaggaagc ggaaaaccat cagaacgtac agtaagcact ttcgcgccgg ggccttcatg
      901 aacaaacaac aaaagacagc cgttaacatg gctaaattca tccaggatca gtccctgctt
      961 cttctggaca ggcttaatga gctggatctg gatcatgagg ctgatttctg cgagaaactc
     1021 catgaggatg cggaacgcct ttaccgaagc ctgtcagcgc gcctgaatga gcagcaggac
     1081 ggtgcgtaat ggctccgttg tgggtattac cggagtaata ccggcatcgc cttaattttc
     1141 attgtcttgg tgcttaatat tattaccccg gtaataccca gaatcccttt attttttctt
     1201 ttttatttat ttttcatgaa gttgcgatga aaatcgcagc tgcgttaggt gtatgtcatt
     1261 ttttagtata taaaacgacc actcccgagt gctggcgccg caggttaagg atgattctgg
     1321 ctaaaaagcg tccttttccg ggaaagggtc atgctcgtat gaccctatgg gttcagcgac
     1381 tacacataaa ctacaaaatt gcatctgctt ttaccccaga acgcgggtgt acaacagaat
     1441 tacatcaaaa gtacatcaaa attacatcac ttttacgtca gatcgcgggt gtacaacaga
     1501 attacatcaa aagtacatca cttttacgtc acttttacat catttttacg tcagatctga
     1561 cctctgaact attcgtacat ccgccccctc ttcctgggcc tcctgcacgt gctgaaaatg
     1621 gtttttccgg ctgtgccggc aacgcctcac ggccacgcaa taaattgcgc atccgttcac
     1681 cgttatcggg gtggttcatg gtggctttca gggcgttcat cggattttag ccgctaaaat
     1741 cccggccagg tatgtccgtg catagccatg cagtcgcgcc agaatcatca ggggcgtttt
     1801 ttctcgatat gggcacagaa aggtgtgtgt ttcaggccgg aggcctcaga aacgccctga
     1861 gggcctctca ggcggtgcgg gcaacggatg ttatggtaaa tccggtggct ttgacgtgcc
     1921 tgccgttgtg taccgtcgtg agtgaaaata attatattgt tcgaagaaca gcgctttttg
     1981 gccggagaaa agcagttctg caaacgatgg tatatcgata tgagttcttt tggcattaat
     2041 ccaggcaaga cgccgggcaa taagcgtaat attccggatg ttacttattt ccatttctgt
     2101 caacagcatc cccccccacg ccggagaata aaaaagccgc ctgttccatc ggcaagccct
     2161 acatcaaggg aaactacgtc ttacgattcg ttttgaaccg ggacgctttg ttgctgcgaa
     2221 tcactaacca gataatccac gttttgtgag tgggtttagg ttaaggcttc aatagaacaa
     2281 taatagcgtt aagtgattag ggcaacctgt gattgctaaa atgatgaagt cttcacatca
     2341 tttcggtggt aatgactcca acttactgat agtgttttat gttcagataa tgcccgatga
     2401 ctttgtcatg cagctccacc gattttgaga acgacagcga cttccgtccc agccgtgcca
     2461 ggtgctgcct cagattcagg ttatgccgct caattcgctg cgtatatcgc ttgctgatta
     2521 cgtgcagctt tcccttcagg cgggattcat acagcggcca gccatccgtc atccatatca
     2581 ccacgtcaaa gggtgacagc aggctcataa gacgccccag cgtcgccata gtgcgttcac
     2641 cgaatacgtg cgcaacaacc gtcttccgga gcctgtcata cgcgtaaaac agccagcgct
     2701 ggcgcgattt agccccgacg tatccccact gttcgtccat ttccgcgcag acgatgacgt
     2761 cactgcccgg ctgtatgcgc gaggttaccg actgcggcct gagtttttta aatggcggaa
     2821 aatcgtgttg aggccaacgc ccataatgcg ggcggttgcc cggcatccaa cgccattcat
     2881 ggccatatca atgattttct ggtgcgtacc gggttgagaa gcggtgtaag tgaactgcag
     2941 ttgccatgtt ttacggcagt gagagcagag atagcgctga tgtccggcag tgcttttgcc
     3001 gttacgcacc accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac
     3061 tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcatca tcaccccgct
     3121 catgagacaa taaccctggt aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3181 tcaacatttt cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3361 ttttccaatg atgagcactt ttaaagttct gctatgtggt gcggtattat cccgtgttga
     3421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3541 tgccataacc atgagtgata acactgctgc caacttactt ctgacaacga tcggaggacc
     3601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     3721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4141 cccttaacgt gagttttcgt tccactgagc gtcagacccc ggaaacgata ggtgcaagca
     4201 agttaagggt tgcatcgcgc atgtcaatct aggctatacc ctaacttgat gtcaggcagg
     4261 gccgcgccgc ttcgtcagaa tagagtctgc tttcccattt tttgacacat gcccgcgaag
     4321 gttatagatt tcagcctgac agaaatgggc tttgaggcac aacggaacag aaagtgcact
     4381 taagccgcct tcaaccaagg agacatcgtg caggggcacc gcatcggcta cgtccgggtc
     4441 agcagctttg accagaaccc ggaacgccag ctggaacaaa cccaggtgag caaggtgttc
     4501 accgacaagg catcgggcaa ggacacccag cgcccccagc tcgaagcgct gctgagcttc
     4561 gtccgcgaag gcgatacagt ggtggtgcac agcatggatc ggctggcccg caacctcgat
     4621 gacctgcgtc gcttggtaca gaagctgact cagcgcggcg tgcgcatcga gttcctgaag
     4681 gagggcctgg tgttcactgg cgaggactcg ccgatggcca acctgatgct gtcggtgatg
     4741 ggggccttcg ctgagttcga gcgcgccctg atccgcgagc ggcagcgtga gggcatcgcc
     4801 ttggccaagc agcgtggcgc gtaccggggc cgcaagaaag ccctgtccga tgagcaggct
     4861 gctaccctgc ggcagcgagc gacggccggc gagcccaagg cgcagcttgc ccgcgagttc
     4921 aacatcagcc gggaaaccct ctaccagtac ctccgcacgg acgactgaca catgccgcgt
     4981 cgcttgatcc tctcggccac ggagcgggac accctgcttg cgctgccgga aagccaggat
     5041 gacctgatcc gctactacac cttcaacgac tccgacctgt cgctgatccg ccagcgacgc
     5101 ggcgacgcca accgcctcgg cttcgccgtg cagctctgcc tgctgcgcta ccccggttac
     5161 gcgctgggaa ccgacagcga gctgcccgag ccggtcatcc tgtgggtggc gaagcaagtc
     5221 caggccgagc cggcgagctg ggcaaagtac ggcgagcgcg acgtgacccg tcgcgagcat
     5281 gcccaggaac tgcgcaccta cctgcaactg gccccgttcg gcctgtccga cttccgcgcc
     5341 ctggacctcg cccgcgagcg ccaggcgcac gaggccggcg cgcggacccg cgccacggcc
     5401 cacgagcgga cgccgcagca ggagcgccag aaggccgcca gagaggccga gcgcggccgt
     5461 gaggcttgga cgctagggca gggcatgaaa aagcccgtag cgggctgcta cgggcgtctg
     5521 acgcggtgga aagggggagg ggatgttgtc tacatggctc tgctgtagtg agtgggttgc
     5581 gctccggcag cggtcctgat caatcgtcac cctttctcgg tccttcaacg ttcctgacaa
     5641 cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt
     5701 ccggacgggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct
     5761 gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg
     5821 ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac
     5881 ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc
     5941 gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc
     6001 gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat
     6061 gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt
     6121 gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac
     6181 cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca
     6241 actttgtcat gcttgacact ttatcactga taaacataat atgtccacca acttatcagt
     6301 gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa
     6361 acccaacaga cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg
     6421 cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac
     6481 cgcccactat ggcattctgc tggcgctgta tgcgttgatg caatttgcct gcgcacctgt
     6541 gctgggcgcg ctgtcggatc gtttcgggcg gcggccggtc ttgctcgtct cgctggccgg
     6601 cgctgctgtc gactacgcca tcatggcgac ggcgcctttc ctttgggttc tctatatcgg
     6661 gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt atattgccga
     6721 tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt
     6781 cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc cccacgctcc
     6841 gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt tccttttgcc
     6901 ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc cgctcgcttc
     6961 gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct tcttcatcat
     7021 gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg atcgctttca
     7081 ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc attcactcgc
     7141 ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg cactcatgct
     7201 cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac ggggatggat
     7261 ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc
     7321 aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct cactggcggc
     7381 gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct atgcggcttc
     7441 tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact tgctctgcct
     7501 gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg
     7561 gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta
     7621 ggagtgcggt tggaacgttg gcccagccag atactcccga tcacgagcag gacgccgatg
     7681 atttgaagcg cactcagcgt ctgatccaag aacaaccatc ctagcaacac ggcggtcccc
     7741 gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac
     7801 caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg
     7861 gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt
     7921 cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg
     7981 cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg
     8041 acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa
     8101 atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac
     8161 acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag
     8221 accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg
     8281 cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc
     8341 ggcaacgccc gcagcatcgc gaccgtcatc ggtgagaagt tcggcaggta ttgggtggtg
     8401 acaatgtagg tgctgcccca aatggcaggt gctatcgctg tgaacaataa atcgggcgtg
     8461 cgtaatgaca tgcgattaca agacctccgc tacggcgatg ggtttttgag agttgcaggc
     8521 gccgcgatga ccgcaccctg acttggactg gccctgcctc gcaccaatcc acctgcggct
     8581 atggccgcga tcatgcgagg cccgaaaatt acacgacctg tacacgtccg ccgaacccgg
     8641 aacgcaacag agcttcgtgg gcatcccgat ccgggtcgta gcagcagtcc tggaccaaac
     8701 gcacgtcgta gtccgcatca ctagcccagg cgacgcttga aagaacaacg ccggtggtgc
     8761 ttatcccggc catgacaagc gtgcttacgc cgcgcgtccg aaggtcggcg tcgagcgccg
     8821 tgccataaaa gacgctggct cgcggacatg catagaaaag gtcgccccgt tcgatcgcaa
     8881 gcccttcgac gggcagaccg gtgcgaaatc gtccgctcgg gagatacggt gagatttggc
     8941 gattcgtcgc cggcggcgca tgttcatact cttcgccgag cgaaaagttg gggaacagca
     9001 cgggccggcc gcgcaaggcg tgctggcagc cgtgcagacc ctgcgtgaga tgaacgccga
     9061 caacctgcgc aaggtgccgg ccgatgcacc cacggccttc atcaagccgc gctggaagcc
     9121 gctggtgatc accccggaag gcctcgaccg gaaattctac gaaatctgcg ccctgtccga
     9181 gctgaagaac gccctgcgct ccggcgacat ctgggtcaag ggctcgcggc agttccgcga
     9241 cttctacgac tacctgctgc cggccgagaa gttcgccgca ctcaagcgcg agcaggccct
     9301 gcccctggcg atcaacccga acagcgacca gtacctggaa gagcgtttgc agctgctgga
     9361 cgagcagttg gccaccgtca cccgcctggc caaggacaac gagctgcccg atgccatcct
     9421 caccgagtca gggctgaaaa tcaccccgct ggatgcggcg gtgccggatc gagcgcacgc
     9481 tgttcatcct ggactggctg caaagtgttg aactgcgccg ccgcgtgcat gccggcctga
     9541 acaaaggtga ggcgcgcaac tcgctggcca gggcggtgtt cttcaaccgc cttggggaaa
     9601 tcagggatcg gagcttcgag cagcagcgct accgggccag cggcctcaac ctggtgacgg
     9661 cggctatcgt gctgtggaac acggtgtacc tggaacgcgc cacccagggg ttggtcgagg
     9721 ccggcaagcc ggtggacggc gagctgctgc aattcctgtc gccgctgggc tgggagcaca
     9781 tcaacctaac cggcgattac gtctggcggc agagccgcag actggaagac gggaagtttc
     9841 ggcccttacg gatgcccgga aaaccttagc gtacgatttt ttccgaattc tgcgggctcc
     9901 cc
//
