LOCUS       NZ_AP027907             2043 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2018-11-1BCC plasmid p2018-11-1BCC_5,
            complete sequence.
ACCESSION   NZ_AP027907
VERSION     NZ_AP027907.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562585
            Assembly: GCF_036508395.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 248x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036508395.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,650
            Genes (coding)                    :: 4,402
            CDSs (with protein)               :: 4,402
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 75 of 248
            Pseudo Genes (incomplete)         :: 170 of 248
            Pseudo Genes (internal stop)      :: 51 of 248
            Pseudo Genes (multiple problems)  :: 44 of 248
            Pseudo Genes (short protein)      :: 3 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027907.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2018-11-1BCC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2018-11-1BCC_5"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2018"
     gene            complement(join(1358..2043,1..283))
                     /gene="mobV"
                     /locus_tag="AABK19_RS23860"
                     /old_locus_tag="VEE49_46030"
     CDS             complement(join(1358..2043,1..283))
                     /gene="mobV"
                     /locus_tag="AABK19_RS23860"
                     /old_locus_tag="VEE49_46030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            862..1296
                     /locus_tag="AABK19_RS23865"
                     /old_locus_tag="VEE49_46020"
     CDS             862..1296
                     /locus_tag="AABK19_RS23865"
                     /old_locus_tag="VEE49_46020"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3..292)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.753"
                     /coverage="96.0"
                     /evalue="5.899999999999999e-54"
     Typing          complement(1362..2041)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.476"
                     /coverage="96.0"
                     /evalue="4.53e-132"
ORIGIN
        1 actccgcctg ctgccgtggt gtggcctctt tccaccactc agggcttgct gtcataacgt
       61 attcaacggc cagtaccgca tctttacggc gtttttccgg cagcagctcc cgcacacgcc
      121 ccattgcctg atccgttgat ttggacacag agcagtagtt ttctggtgtc cgttcagcgt
      181 tagcgttcgg cgtctcacgc tctcggtagc agtgctgcaa cgcgctggcc acgcttccca
      241 ttcccgtcag ctttttgcag cgcattatcg cgtagttcgc catgttcccc cctgattccg
      301 gcattcattc agccaggcgg gggagcgcat tgcgatacct ccggcgggcc actatcaaga
      361 tgatagtgta ctcactagac tttgctacgc aaaatcgttc gggcagcaag ctgccagaag
      421 gtcaaaatca acggcggtct ggcaggtgga aggcgtcgct ccgcgtcacc tcccaccact
      481 gccaagcctg ttttatttgt gcgtcgtcaa gggtgaaatg cactcgctcc gctcgccctt
      541 gacgaatgtc aggcaacaaa gtcgtctttg aaagagcacg cgccgctcgc cgcaggtcag
      601 gctgaggcgt accgcagcgc agcgaggaac gaacagcctg aacgaggaag cggaatatca
      661 cctgaacggg catagaaatg cttacgcacg aaccgatgaa aatagggttc gctatcctgg
      721 catatgagct tctcgctata agatacagaa gagagccttc tctcagccct tgtggggcgc
      781 ggctctcgct tgacgatagt tatcattatt gataacgttg ttatcattat tgataactat
      841 ttttttggga gagcctctta aatgagtaac cgaattactc agatagacac tgctacaggg
      901 gaagaggtag gtagttttgt cgcagttatc cgacccaaac agaagtcagc ttttgagagg
      961 catttcacta tgaaccaaga cgctttaaag atccttgcta agaccctcac aggggagcaa
     1021 ttcaaagtct tgatgatgct gttagctgat ctcgattatg aaaattacat ccaaattgcc
     1081 caagccgata ttgccgacgc tttaggtatg cagaaaacca atgtaagccg ttcggttaag
     1141 gcgctaattg acctatccgt gattcttcaa ggcccaaaag tagggcgcag ttacagttac
     1201 cgtctgaacc ctcagtttgg ctggaaaggt actgtcagca accacaagaa agcactaaaa
     1261 aacggcctca gcgttataca gggcggcaga acctaatctg gaatgccgca aatgacgtca
     1321 ctttgcggcg ttagccctat cctgcatcgc atcaaaacta acgctccata ccacccatgc
     1381 cacgcgatcg cgtctgtcgc tgtttttcac gttcaatacg ctgctgtttt tccttttcct
     1441 ggttttgctg cttaaaccgc tgagccatca tcaggatttc ggtcatctgc tctctggaaa
     1501 gccccttaaa aggctctgtg acgctctgta ggcgtttctg ctgcgcatcc atagtcttct
     1561 gtagctcacg cgctcttttt gcgttctggg cgctctgaga ggccatagcg atggttcctg
     1621 caaagccatc gttcactgcg tggcttagac gtttggctat ggctgcctga tcttccacgt
     1681 ctttggtgaa aatgcccttc cgcaatacac gcggctccag cgcctccggc gagatcgata
     1741 cctggctacg ggtgccacgg tttatcgatt cgtagtagtg ctgaactgtc tgatgcgtgg
     1801 cgcggcttcc ctcgatgccg cgctcaagac ccagtttttt tactgattca gcgtaggtgc
     1861 tctgatcttc acgcatcttt gaccgcccgc caataaactc tttcgcggac agccgtccat
     1921 cctgcgtcaa tggcaccacg aacgcagaca ggtgcggggt ggcctcgtcc cggtgaacca
     1981 ccgccgccac aacacgatcc ttcccgtatt tcttctccag ccattgctca gagcgggcga
     2041 aaa
//
