LOCUS       NZ_AP027906             2138 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2018-11-1BCC plasmid p2018-11-1BCC_4,
            complete sequence.
ACCESSION   NZ_AP027906
VERSION     NZ_AP027906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562585
            Assembly: GCF_036508395.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 248x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036508395.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:27:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,773
            CDSs (total)                      :: 4,650
            Genes (coding)                    :: 4,402
            CDSs (with protein)               :: 4,402
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 75 of 248
            Pseudo Genes (incomplete)         :: 170 of 248
            Pseudo Genes (internal stop)      :: 51 of 248
            Pseudo Genes (multiple problems)  :: 44 of 248
            Pseudo Genes (short protein)      :: 3 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027906.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2018-11-1BCC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2018-11-1BCC_4"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2018"
     gene            join(1860..2138,1..717)
                     /locus_tag="AABK19_RS23855"
                     /old_locus_tag="VEE49_46010"
     CDS             join(1860..2138,1..717)
                     /locus_tag="AABK19_RS23855"
                     /old_locus_tag="VEE49_46010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_038989441.1"
                     /translation="MQNPKLKNLTDYSPDDKPWDVHKSQSDDVGGIYLRAAEFEAYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDARWMFLTLTVRNCAIEDLGDTLTAMNAAFQRMKVRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPGMLNGHDYVKHYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPDWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDPD"
                     /name="protein rep"
     Typing          complement(438..628)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.375"
                     /coverage="98.0"
                     /evalue="1.4200000000000001e-44"
ORIGIN
        1 gcccgttttt atcagtctct gccgaaaatc gtcgctgatt accctgatgc ccgctggatg
       61 ttcctcacgc tcaccgttcg caactgtgcg atcgaggatc tcggggacac gcttactgcc
      121 atgaatgccg cattccagcg catgaaggtt cgcaaggaat ttaaatccgt acagggatgg
      181 attcgcacca cagaagttac ccgcggtcgt gatggctctg cacaccccca ctttcacacc
      241 ctgatgatgg ttcctcctgg gatgctgaac ggtcatgact acgttaagca ctaccgctgg
      301 gttgaactct ggcgtgactg cctgcgcgtt gattacgatc ctaatgttga tgttcgggca
      361 gtgaaacccc gcaagcctaa agatggccag actctggcat gtgccactgc tgaactggtg
      421 cgcggtgccg ttgctgaaac cctgaaatat tcgacaaagc cggctgatat ggttgccgac
      481 ccggactggt tcttagagct gacccggcag acgcataaac gccgttttgt tgcgaccggt
      541 ggcgctctga aagacgttct gaaactggag caggaaacgg atcaggacat ggtgatcggg
      601 gatgatattt ccgatggtga agacgatggt tcacgcctcg cctttggctg ggattcgacg
      661 ccaagaaaat acgtccgttt gcctgaacgc gataagttta aatcagatcc tgattagggt
      721 gtcggggcct gccctgacca ggttgttttt ggatgccgct gcgtgtggcg gtactccaaa
      781 aacacatctt gtccggtaca cagatttcct ttggcattcc ttcgggtttc cctgagattt
      841 ccactctgca tacatacacg catacacgaa taatcaacgg cagaattcgg gcaggatatg
      901 taaagtggga ccactttacg cctgccttat tcgcactctg ctcaatggaa tattaaatct
      961 gacagcgttc gccgtcgtcg aatcgtcaac ttctaaatcg ggggatccct ttagggttcc
     1021 gatttagtgg ttgtacgact gcacccctca tacccttgtg atgggtgatg gttcacatgg
     1081 tgagccatcg ccctgtaaag ccgcttttta cccccttcaa ggcgttgcct gtggggtagg
     1141 tgcgtaagcc ctgccctaca ggtgatgccg tgggggtatt ttcgttttgc tcggtatgcc
     1201 ttattcgttt aaatccgcca tcaggagtat gcggggtaaa gtcgtgcggc cgcgatgagg
     1261 aacggggatt gcccccgcag ctctgcgagg aggcacacgc cgttcggaat cggggtccac
     1321 tctgtttaat agtggactct tgttgcgatt tgcaacggaa acagccgcgt gcagtgaatg
     1381 taatactgca cgtttgtctc aatctgacat ttctcagggg ggggcgattt aacgtggttt
     1441 gcttaatttt tgagcttacg aatgcttgag tgtttgagtg cctgaatgct gcttttgggg
     1501 gttggttttt cggccctgaa aggggcgaaa tccttgtatt tatacttaag ggataactct
     1561 aggattcaaa ttcgcttcca gcccttgtgc cgcaaggcct gagagccaat ttgctcgaaa
     1621 atggttttgt gagtatgatt tctgacaggt gatcgtgtcc gcgaactaat ttttgattac
     1681 ttggggtggt tcccaagacc ttacttactg acacgatgat atgagaaacc cctgcaagtg
     1741 ctaccaacac ttcaggggcg cgatcacctg gctaaatacg gtttgaaatc gactcgcaac
     1801 aggtgatacg gaaatgataa cacacccttc cagcgcctgc ccatattgga gcaggtgcta
     1861 tgcagaatcc taagctaaag aaccttactg actacagtcc cgacgataag ccgtgggacg
     1921 ttcacaagtc gcaatctgat gatgttggcg gtatctatct gcgtgcagct gaatttgagg
     1981 cgtatgcggc tcgtatgcgc gattgtggcg gcttgctgcg ttttggctgg tctaccctta
     2041 aagacaccgg agaaacgcgt ttacggcttc gtgaggcgca tttctgccgc gttcgtcact
     2101 gccctgtctg ccagtggagg cgttccctga tgtggcaa
//
