LOCUS       NZ_AP027892             2442 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2018-06-4CC plasmid p2018-06-4CC_3, complete
            sequence.
ACCESSION   NZ_AP027892
VERSION     NZ_AP027892.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562621
            Assembly: GCF_036507815.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 596x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036507815.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:06:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,719
            CDSs (total)                      :: 4,600
            Genes (coding)                    :: 4,407
            CDSs (with protein)               :: 4,407
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 65 of 193
            Pseudo Genes (incomplete)         :: 116 of 193
            Pseudo Genes (internal stop)      :: 35 of 193
            Pseudo Genes (multiple problems)  :: 24 of 193
            Pseudo Genes (short protein)      :: 5 of 193
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027892.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2018-06-4CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2018-06-4CC_3"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2018"
     gene            complement(join(1561..2442,1..87))
                     /gene="mobV"
                     /locus_tag="AABK02_RS23580"
                     /old_locus_tag="VEE72_44960"
     CDS             complement(join(1561..2442,1..87))
                     /gene="mobV"
                     /locus_tag="AABK02_RS23580"
                     /old_locus_tag="VEE72_44960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            792..1274
                     /locus_tag="AABK02_RS23585"
                     /old_locus_tag="VEE72_44940"
     CDS             792..1274
                     /locus_tag="AABK02_RS23585"
                     /old_locus_tag="VEE72_44940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            1261..1491
                     /locus_tag="AABK02_RS23590"
                     /old_locus_tag="VEE72_44950"
     CDS             1261..1491
                     /locus_tag="AABK02_RS23590"
                     /old_locus_tag="VEE72_44950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_140424895.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHGYHLND"
                     /name="hypothetical protein"
     Typing          complement(1565..2442)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.222"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 acgctctcgg tagcagtgct gcaacgcgct ggccacgctt cccattcccg tcagcttttt
       61 gcagcgcatt atcgcgtagt tcgccatgtt cccccctgat tccggcattc attcagccag
      121 gcgggggagc gcattgcgat acctccggcg ggccactatc aagatgatag tgtactcact
      181 agactttgct acgcaaaatc gttcgggcag caagctgcca gaaggtcaaa atcaacggcg
      241 gtctggcagg tggaaggcgt cgcttcgcgc caccccccac cactgccaag cctgttttat
      301 ccggagtgtc gtcaagggta aatggcactc gctacgctcg cccttgacga aatcaggcaa
      361 tgaaatcgtc tttgaaagag caggaaccgc tcgccgcagc tttggctgag gcgtaccgca
      421 ggagcaacgc gacgaggaac gaacagccaa agcgaggaag cggaatttcc actccccgta
      481 catagaaatg cgtacactcc ttgctaaaaa aaggcagttt ttcagtgtct aaaaatgccg
      541 tttttcagcc cttattttcc cccttaatat atagatatag atatgggaac ccgcttgtac
      601 caagggctag agcgaagaat cgtactcacc tcaagtacca atcgtactca cctcaagtac
      661 taatcgtact cacctcaagg actaatcgta ctcacctcaa gtactaatcg tacttgatac
      721 aaaggacttg actgtatgtc tctttagtac ttaaatgcga gtactaaatc atggcgaaac
      781 gtggggctgg agtgaatacg aaaaagattt ctaggcttga acggcataca tacatggatg
      841 aaaacggtga ggtaaaacaa gactctaaga tcgagacctt ttccatccca gccgaacctc
      901 cctacgtcaa gctatacatt gcagacattg cgatgattca tggtttatca aaatctcaaa
      961 atgatgtgct tttacaattg gcaatgctga ttggatggga tgggtttgtg tctgtatcaa
     1021 aaactagatt tgaaaaaacc atacagccca gggttcatat tgaatatcag acctttaaaa
     1081 acataattgc ggctcttgta gataaacaaa tattcttccg ggcagggcgt ggggaacttg
     1141 aagctaaccc ctttcttttt gcacggggag actggccatc aatttacgaa agaagaaaga
     1201 aaattgagtt aaagatcagt tatgacacca atggaagaac ggtgcagacg aaggtaatgg
     1261 atgatgaatg ctgaagataa actttttaaa aatgtagaca ggctaatgct gcatgattca
     1321 ctaaaaaaga atgaggttct gactgtttgg gagcgtactt ttttaagcag tgtttacagc
     1381 atatatcgta gaactagcgg taataaatac tcaactaagg gtttgtcacc aaagcagaaa
     1441 aacgcagctt tttctatttt aaaaaaacat ggttaccatc tgaatgatta attttttcgt
     1501 ggtgaatgcc gcaaatgacg tcactttgcg gcgttagccc tatcctgcat cgcatcaaaa
     1561 ctaacgctcc ataccaccca tgccacgcga tcgcgtctgt cgctgttttt cacgttcaat
     1621 acgctgctgt ttttcctttt cctggttttg ctgcttaaac cgctgagcca tcatcaggat
     1681 ttcggtcatc tgctctctgg aaagcccctt aaaaggctct gtgacgctct gtaggcgttt
     1741 ctgctgcgca tccatagtct tctgtagctc acgcgctctt tttgcgttct gggcgctctg
     1801 agaggccata gcgatggttc ctgcaaagcc atcgttcact gcgtggctta gacgtttggc
     1861 tatggctgcc tgatcttcca cgtctttggt gaaaatgccc ttccgcaata cacgcggctc
     1921 cagcgcctcc ggcgagatcg atacctggct acgggtgcca cggtttatcg attcgtagta
     1981 gtgctgaact gtctgatgcg tggcgcggct tccctcgatg ccgcgctcaa gacccagttt
     2041 ttttactgat tcagcgtagg tgctctgatc ttcacgcatc tttgaccgcc cgccaataaa
     2101 ctctttcgcg gacagccgtc catcctgcgt caatggcacc acgaacgcag acaggtgcgg
     2161 ggtggcctcg tcccggtgaa ccaccgccgc cacaacacga tccttcccgt atttcttctc
     2221 cagccattgc tcagagcggg cgaaaaactc cgcctgctgc cgtggtgtgg cctctttcca
     2281 ccactcaggg cttgctgtca taacgtattc aacggccagt accgcatctt tacggcgttt
     2341 ttccggcagc agctcccgca cacgccccat tgcctgatcc gttgatttgg acacagagca
     2401 gtagttttct ggtgtccgtt cagcgttagc gttcggcgtc tc
//
