LOCUS       NZ_AP027880            10032 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2018-05-2CC plasmid p2018-05-2CC_3, complete
            sequence.
ACCESSION   NZ_AP027880
VERSION     NZ_AP027880.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562579
            Assembly: GCF_036507355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 234x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036507355.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:07:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,599
            Genes (coding)                    :: 4,319
            CDSs (with protein)               :: 4,319
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 81 of 280
            Pseudo Genes (incomplete)         :: 182 of 280
            Pseudo Genes (internal stop)      :: 54 of 280
            Pseudo Genes (multiple problems)  :: 35 of 280
            Pseudo Genes (short protein)      :: 4 of 280
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027880.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2018-05-2CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2018-05-2CC_3"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2018"
     gene            join(8887..10032,1..1245)
                     /locus_tag="AABK05_RS23395"
     CDS             join(8887..10032,1..1245)
                     /locus_tag="AABK05_RS23395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="WP_338388434.1"
                     /translation="MQFRFESTKIVVVELWGFGALVVKHFLKAHKPRLSNSLRYNMKFR
                     VFCLAALPCFSVSAQSLENLGSLTADQQGASSATAVSADGSTVVGEAAYDLTAGGTGRH
                     AFRWTSAGGMQDLGDLTANQQGYSSATAVSADGSTVVGGAAYDRTAGGTGRHAFRWTSA
                     GGMQDLGDLTANQQGYSSATAVSADGSTVVGGADYDRTAGGTGRHAFRWTSAGGMQDLG
                     DLTADQQGASSAYAVSADGSTVVGGANYDRTAGGTDYHAFRWTSAGGMQDLGDLTANQQ
                     GYSHATAVSADGSTVVGWANYDLTAGGTGRHAFRWTSAGGMQDLGDLTADQQGASSAYA
                     VSADGSTVVGWANYDRTAGGTDYHAFRWTSAGGMQDLGDLTADQQGYSSATAVSADGST
                     VVGGADYDLTAGGTGRHAFRWTSAGGMQDLGDFTSDQQGSSSATAVSADGSVVAGIASN
                     DAGEMRAFIYKAPQSPTDPNLPIQDYENVLKSFPKLAADKELAAAQQQLGMQRLLTSQC
                     SAGQAEQSCLVVSGSLDTAGSDGPIGRRQQTAGTVTLGRGLDERWTVGASLSLAHSSLH
                     HNAVNPKNAYGVSGWVNYSEHGLSGLGWQMDAAIGYAKNKSTITRGQGLEHVHLVQGTS
                     NLDTVAARIALGFGIQQRHGWTLTPAVSVTQQNSKFAAYSESPSAFTASYDKSELKTTV
                     LGVDLSAKKSLSDKSQLRLAVGVEQDLKTDRMKLAGVSDVPGAQRFNLESALERKKLRP
                     YASVGYRYQVSAQGAVFVNTGVMRDMFSNKPNVGVQVGYSARF"
                     /name="autotransporter domain-containing protein"
     gene            1384..1527
                     /locus_tag="AABK05_RS23400"
                     /old_locus_tag="VEE46_45080"
     CDS             1384..1527
                     /locus_tag="AABK05_RS23400"
                     /old_locus_tag="VEE46_45080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866074.1"
                     /translation="MSVSLRLPPELNQRLSNLAERELEAVRAGKSKAVPLEDVMKQYGL
                     AD"
                     /name="hypothetical protein"
     gene            1511..>1708
                     /locus_tag="AABK05_RS23405"
                     /old_locus_tag="VEE46_45090"
                     /pseudo=""
     CDS             1511..>1708
                     /locus_tag="AABK05_RS23405"
                     /old_locus_tag="VEE46_45090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1708..1947
                     /locus_tag="AABK05_RS23410"
                     /old_locus_tag="VEE46_45100"
     CDS             1708..1947
                     /locus_tag="AABK05_RS23410"
                     /old_locus_tag="VEE46_45100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866072.1"
                     /translation="MTQDKWLAERLAYLRGLKAPSDQQRLLLMLADKPDRTADAVRIIN
                     AEKAAERKARPTICRPASPCPSKAVAASRCLRQP"
                     /name="hypothetical protein"
     gene            complement(2447..3442)
                     /locus_tag="AABK05_RS23415"
                     /old_locus_tag="VEE46_45110"
     CDS             complement(2447..3442)
                     /locus_tag="AABK05_RS23415"
                     /old_locus_tag="VEE46_45110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="WP_257866071.1"
                     /translation="MSKSNGPQPIGSLLGNIVPREVKRAADATASKGQSNKSLSFISRQ
                     KINLIDAAAEIATNPPSGEDMAFTHAVLCQVGLPRAKVEGREFMRQSGAAWVNVQAGYL
                     DEGNGPVLQPIPYGVMPRLALAWVSTFAVRNKEREIKIGDSAAEFLSLMGLDDQGARYA
                     TLRKQMHALAACRLQLGFKGRTFNGQPVEQFDAWMANRDTKQRALWPGTMVLSEGYFTS
                     LMQSAVPLDNRALMALKGSALALDVYAWLAHRLHRIEGRGVTLHWKSLREQFAQEYTGK
                     DADKDFKKKFVPALKKVLAVYPQAKVKPVTGGVLLLGSPPPISYKGGPTE"
                     /name="replication protein RepA"
     gene            complement(3470..3727)
                     /locus_tag="AABK05_RS23420"
                     /old_locus_tag="VEE46_45120"
     CDS             complement(3470..3727)
                     /locus_tag="AABK05_RS23420"
                     /old_locus_tag="VEE46_45120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005918078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866070.1"
                     /translation="MATKKPADLTGLVATKGSAAPVADMPTCTPKVKAEAEASANNVPL
                     NFRVSAELRRRFRMFAAAHDLKLNELLRLAFDEYEQRHNN"
                     /name="hypothetical protein"
     gene            complement(3729..4379)
                     /locus_tag="AABK05_RS23425"
                     /old_locus_tag="VEE46_45130"
     CDS             complement(3729..4379)
                     /locus_tag="AABK05_RS23425"
                     /old_locus_tag="VEE46_45130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400008.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_257866069.1"
                     /translation="MKILVLASQKGGAGKTTLAAHLAVAAEQAGAGPAVLLDTDPQGSL
                     SAWWNSREDDTPALAAASLAELPAKLRALAEAGFKLAVIDTPPAITEAIRDVVKLADLV
                     LIPTRPSPHDLRAVGSTVDIAQETGRPFAFAVTQAKPTARLTVQAVAALSAHGAVAPAI
                     VHDRVDYAASMVDGRTVGESDPKSRSAEEISQLLAFVQERMNAKKITKKKETV"
                     /name="ParA family protein"
     gene            complement(4558..5190)
                     /locus_tag="AABK05_RS23430"
                     /old_locus_tag="VEE46_45140"
     CDS             complement(4558..5190)
                     /locus_tag="AABK05_RS23430"
                     /old_locus_tag="VEE46_45140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477436.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_257866068.1"
                     /translation="MAASKVARVYIRVSTDEQDVRRQESIIESAKAAGYYVAAVYREKA
                     SGARADRPELLRMIADLQAGEVVIAEKMDRISRLPLPEAEQLVQAIRDKGARLAVPGVV
                     DLSELTETAEGVARIVLQSVQDMLLRLALQMARDDYEDRRERQRQGIELAKAAGRYRGR
                     KPNRALHARVVALREGGSSIAETARLAGCSESQVKRIWASRRQIPAA"
                     /name="recombinase family protein"
     gene            complement(5202..5456)
                     /locus_tag="AABK05_RS23435"
                     /old_locus_tag="VEE46_45150"
     CDS             complement(5202..5456)
                     /locus_tag="AABK05_RS23435"
                     /old_locus_tag="VEE46_45150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_257866067.1"
                     /translation="MRLVMTLAAVAVLTACNPASSDEAADARPVHDVAYFLDHPDERKA
                     QIAECRNNPGELADTPNCKNAMAAQQKAMFRGTGMPSIR"
                     /name="EexN family lipoprotein"
     gene            complement(5585..5737)
                     /locus_tag="AABK05_RS23440"
     CDS             complement(5585..5737)
                     /locus_tag="AABK05_RS23440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866066.1"
                     /translation="MKKAGRQSRPTFRLLAAGLAFALRRVEDGPAKREHGFWIVQSKSR
                     GGRRV"
                     /name="hypothetical protein"
     gene            6213..6986
                     /locus_tag="AABK05_RS23445"
                     /old_locus_tag="VEE46_45160"
     CDS             6213..6986
                     /locus_tag="AABK05_RS23445"
                     /old_locus_tag="VEE46_45160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004668256.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_257866065.1"
                     /translation="MALAHKAQGYDVADVERPARERWVTMSNALTRAGHGLTLAEKRIV
                     AAAVSKLGSRRVLKPGEVPRTKITAAEYAETFEVDMNTAYDQLQGAAKQLYNRSITFYE
                     PAHKRNGKPLPPTRVQMRWVGSVKYQDGEGWVELAWWPDLLRHLTGLQRQFTTYQLQQA
                     SALRSGYSWKLLELLMRFESTGWAEYTIEDFKASMDAPPSLSDFGQVKRRIIDPAVKEL
                     TEKDGWLIQWQPIKAGRKVKALRFTFMRDPQGRLL"
                     /name="replication initiation protein"
     gene            complement(7079..8599)
                     /locus_tag="AABK05_RS23450"
                     /old_locus_tag="VEE46_45170"
     CDS             complement(7079..8599)
                     /locus_tag="AABK05_RS23450"
                     /old_locus_tag="VEE46_45170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_338383265.1"
                     /translation="MAIARLSMKVGKAGKAGPHAAYIAREGQYANRLERGERLEATEAG
                     NMPASAQSNPLAFWQAADAYERKNGTTYREMEIALPRELDAEQRAALVREFVRQEIGDR
                     HAYQWAIHVPTAADGGEQPHVHLMFSERQRDGIERDPEQYFKRYNAKAPEKGGARKGYG
                     PSAGQTLTKAERAAELKELRRRWEAMCNAHLERAGVEQRIDMRSHAERGTGLEPERKQL
                     PSQWRGQGRANVIEFRQARAELAEARQELARAEVIHLEAERQFEAMPAAELVEKWDTTT
                     KELAKGYRQRADRLEHRLRTEIHDIGDKRYRARVDHAEKRPAEVKDTLWNRITGKVAEY
                     GKALADWQGTMKRIEGWKQRREADLMRRLKRLEGYLSRTHYRDKATTKAERTLARKYPA
                     WAQRVPAAREQVAAERAQQRALQQAAQGMAKQIVSLAAAHQKGRVQGVSRELGRVLDAV
                     NAMPGRSSQKAATLAAELRERPDLMQTYQPLLAPHQRTLQRSRGGMER"
                     /name="MobA/MobL family protein"
     Typing          complement(3049..3199)
                     /name="ColRNAI_rep_cluster_1998"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.842"
                     /coverage="100.0"
                     /evalue="2.97e-40"
ORIGIN
        1 gaccaacagg gatacagctc tgccactgcc gtgagcgcgg acggcagcac ggtcgtgggg
       61 ggggctgatt acgatctcac ggcaggcggg acgggcaggc atgcatttcg ctggaccagt
      121 gcgggcggca tgcaggattt gggcgacttt acgtccgacc aacagggatc cagctctgcc
      181 actgccgtga gcgcggatgg cagcgtggtg gcgggaatcg caagcaatga cgcaggagaa
      241 atgcgtgcat ttatttacaa agcgcctcag tcgccaacag accctaatct accgatccaa
      301 gactacgaga atgtcctcaa gtcgtttcct aagttggccg ccgacaaaga gttggctgct
      361 gcgcagcagc agctaggtat gcagcgcttg ttgacttcgc aatgttcagc agggcaagcg
      421 gagcaaagct gcttggtggt ctctggctcg ttggatacgg caggctctga cggcccaatt
      481 ggtcgccgtc agcaaacagc ggggactgtc acactagggc gaggtcttga tgagcgctgg
      541 acggtggggg caagcttgtc gctggcgcac tcgagcttgc accacaacgc agtaaatcct
      601 aagaatgctt atggcgtaag tggctgggtg aactacagcg agcatggctt gtccggtctt
      661 ggctggcaga tggatgcagc cattggttat gcgaagaaca agagcaccat cacccgtggt
      721 cagggccttg agcatgttca cctggtgcag ggcacaagca atttagacac agtggcagct
      781 cgtattgcct tggggtttgg catccagcag cgtcatggat ggactttgac gccagcagtg
      841 tctgtgacac agcaaaacag caagtttgca gcttacagtg agtcgccttc agcctttact
      901 gcgtcgtacg ataaatcgga gcttaaaacg actgtgttgg gagtggatct gagtgccaag
      961 aagtctttga gcgacaagtc gcagctgcgt ttagcggtag gcgttgagca agaccttaaa
     1021 acggatcgta tgaagctggc tggcgtctct gatgttccag gagcgcagcg atttaactta
     1081 gagagtgctt tagagcgcaa gaaactgcgt ccttatgcct ctgtgggata ccgctatcaa
     1141 gtcagtgcac agggggctgt gtttgtgaac acaggcgtga tgcgggatat gttctcgaac
     1201 aaacccaacg tgggtgtgca ggtcggttat agcgcacggt tttaacgcga catagcgttc
     1261 aaacatttcc tcagctcacg gtaaatcctg cttatcgatg gcaacaaaaa aggcgctcaa
     1321 agcgcctttt ttgcgtctgg tgctaccatg cgagctacat gtatctcaaa aggagttgat
     1381 gccatgtctg tatctcttcg cctaccccca gaactgaacc agcgtctgag caacttggcc
     1441 gagcgtgagc tggaagcggt gcgtgccggt aaatccaagg ccgtgccttt ggaagacgtt
     1501 atgaagcagt atggcttggc agattgaact gtccgaaggt gctgtcaaaa acctcaagca
     1561 gctcgaccac caacacgccc gacgcatcct gagctttttg cacggtcgcg tggcgcaact
     1621 tgatgacccg cgcagcattg gcgaggcact caaaggctcc aagctggggg agctttggaa
     1681 gtactgcgtg ggggactacc gaatcatatg acacaagata agtggctggc cgaacggctg
     1741 gcatacctgc gcggcttgaa ggctcccagc gatcaacagc gcctgctgct gatgctggcc
     1801 gacaagcccg accgcaccgc cgacgccgtc cgcatcatca acgccgagaa ggccgccgag
     1861 aggaaggccc gtccgacgat ctgccgaccc gcatcaccct gcccaagtaa ggccgtagcc
     1921 gcttcgcggt gccttcggca gccttgaccc ttcccctacg ctcaaaacgc gctgtaggcc
     1981 cttctacggg ccttggcgac cccttccctt cctgattgcc gaagccggag ggggagccgc
     2041 cgccgcaggc ggtgggggaa ccccgcaggg gtgccccatg tccttgtcgc agcagcgttt
     2101 tccacaggtt gtggcggcca attacaggtt tagatttata ggattctttt tttaaaggct
     2161 taacctatcg gtttaggggg ggtccgattt aacgggaaaa agggggtccg atttaacgtg
     2221 gataagtacc cttggggggg tccgatttat cgaggtgggg gggtccgatt taacgtggat
     2281 aagtggcgct atcgacaggg ttgtgcaccg ttttggtccc gctggggttt tcggacactt
     2341 ccagagacat aaaaccccgg agattcaatg cgttgcacac gaatcgccgg ggtctagggg
     2401 gggccgatct aacgacacgt ttgtcggaca cttcaagggg ttagcgttac tcggtcggac
     2461 cgcccttgta agagatcggc ggcggggaac cgagcagcag gacgccgccc gtgaccggct
     2521 tcaccttggc ctgcggatag acggccagaa ccttcttgag ggccggcaca aacttcttct
     2581 tgaagtcctt gtcagcatcc ttgccggtgt actcctgcgc gaactgctcg cgcagagact
     2641 tccagtgcaa agtgacgccg cgtccctcga tgcgatgcag ccggtgggcc agccaagcgt
     2701 agacatcgag cgccagcgcc gaacctttca gggccatcag ggcgcggttg tcgagcggta
     2761 cggcgctttg catcagtgac gtgaaatagc cctcggacaa caccatcgtg cccggccata
     2821 gcgcccgctg cttggtgtcg cggttcgcca tccatgcgtc gaactgctcg accggctgtc
     2881 cattgaaggt gcggcccttg aagccaagtt gcagacggca ggcggccagc gcgtgcatct
     2941 gcttgcgcag ggtcgcatag cgcgcccctt ggtcgtccaa gcccatcagg ctaaggaact
     3001 cggccgcgct atcaccgatc ttgatttcgc gctccttgtt gcgcacggca aaggtggaca
     3061 cccacgccag cgccagccgg ggcatgacgc cataggggat cggttgcagc accggcccgt
     3121 tgccttcgtc cagataaccg gcctgcacgt tcacccacgc ggccccggac tggcgcatga
     3181 actcgcgccc ctcgaccttg gcgcggggca agccgacctg acaaagcacg gcgtgagtaa
     3241 acgccatgtc ctcgccgctc ggcgggttcg tggcgatttc ggcggccgcg tcgatcaggt
     3301 ttatcttttg gcgactgatg aatgacaggc tcttattgct ctgcccttta gatgccgtag
     3361 cgtcggccgc ccgcttaacc tcgcgcggca caatgttgcc aagcagcgaa ccgatgggct
     3421 gggggccgtt agatttgctc ataaggcgat ctccaccggc atggccgggc tagttattgt
     3481 ggcgctgctc gtattcatcg aaggccagcc gcaacagctc gtttagcttg aggtcgtggg
     3541 ccgctgcgaa catgcggaaa cgtcgacgca gctcggccga cacgcggaaa ttgagcggca
     3601 cattgttcgc cgaggcctcg gcctcggcct tcaccttggg ggtgcaggtc ggcatgtcgg
     3661 caaccggcgc ggcgctgccc ttcgtggcaa caaggccggt caaatcagcg ggcttcttgg
     3721 tagccatatt aaacggtttc cttctttttg gttatcttct tagcgttcat gcgttcttgc
     3781 acgaaagcta aaagctggga gatttcctcg gcgctgcggc tcttggggtc gctctcgcct
     3841 acggtgcggc cgtccaccat cgaggcggca tagtccacgc ggtcatgcac gatggctggg
     3901 gctacagccc catgcgcgga caaggcggcc acggcctgaa cggtaagccg cgcggtcggc
     3961 ttggcctgcg tcacggcgaa ggcgaaaggc cggccggttt cctgcgcaat gtccacggtg
     4021 ctgcctacgg cccgcaggtc gtgcgggctg ggccgggtcg ggatcaacac aaggtcggcc
     4081 agcttcacca cgtcgcggat agcctcggtg atggccggcg gggtgtcgat cacggccagc
     4141 ttgaaaccag cctcggccag cgcccgcagc ttggcgggca gctcggccag cgaggcggcg
     4201 gccagtgccg gggtgtcgtc ctcgcgggag ttccaccacg ccgaaagtga cccttgcggg
     4261 tcagtgtcga gcaggaccgc cgggccggct cctgcctgtt cggcggcaac cgcaaggtgc
     4321 gccgctaggg tggttttccc ggctcccccc ttctggcttg ctagaacaag gattttcaca
     4381 tgcgccctct tgattcgtgt tgggacgctt taatgatagc caaaaagcac gcatcttgca
     4441 gagtctattg aacaaaagca cgcttgcgtg ctttctggat tgtgcaatcc aaaagccggg
     4501 gcggccggcg cgtgtaatcg caaacgggta gggaagggga ggccggtttc tgatgcccta
     4561 tgccgccgga atctgccggc gggaggccca tattcgtttg acctggctct cgctgcatcc
     4621 ggccagccgg gcggtttccg cgatgctcga tccgccctca cgcaaggcca cgacgcgagc
     4681 gtgtagcgcc cggtttggtt tcctacctct gtagcggccg gcggccttgg caagctctat
     4741 gccctgccgc tgccgctcgc ggcggtcctc gtagtcgtca cgcgccatct gcagggcgag
     4801 gcgcaagagc atgtcttgca ccgattgcag gacgatgcgg gccacgccct cggccgtctc
     4861 ggtcaactct gacaggtcca ccacgccggg cacggcgagc cgcgcgccct tgtcgcggat
     4921 cgcctgcaca agctgctcgg cctcgggcaa gggcaggcgg ctaatgcggt ccatcttctc
     4981 ggcaatgaca acctcgccgg cctgcaagtc cgcgatcatg cgcaacagct cgggccggtc
     5041 ggcgcgcgcg ccggatgcct tctcacgata gacggcggcc acgtagtagc cggccgcctt
     5101 cgcgctctcg atgatggatt cttgccggcg cacgtcctgc tcgtcggtgc tgacgcggat
     5161 atagacccgt gcgaccttgg acgctgccat tttggtgcct cctaccggat gctcggcatt
     5221 ccggtgcccc ggaacatggc tttctgctgg gccgccatcg cgttcttgca attgggcgtg
     5281 tcggccagct cgccggggtt gttccggcat tccgcgatct gcgccttacg ctcgtcggga
     5341 tggtcgagga aataggccac gtcatgcacg gggcgggcgt cggccgcttc gtcgctgctg
     5401 gcaggattgc aggccgtcag cacggccacg gcggcgagcg tcatcacaag gcgcatagtt
     5461 tctcctttgg cgcagttggg tattcctaaa tgcgcccagc atagcagcta gggcgcattt
     5521 ggtgaatccg cgatgctatt gcgctaggct cgcaaagccg atccatctgc gccagcgttt
     5581 gcgattacac gcgccggccg ccccggcttt tggattgcac aatccaaaag ccgtgttcgc
     5641 gcttcgccgg gccatcctcg acgcggcgaa gcgcgaacgc gaggccagcg gccaataggc
     5701 gaaaggtcgg ccggctctgc cggccggctt ttttcatgga atcggatccc ggtcgccggc
     5761 cggggattcc ttcaacgtgt aatcggtcac gctagaaagc cataggacgc gctaggagcg
     5821 ccgcaccggc cgggccgcta ccttccccct cccctgccct aaagaacgcc gccacgggct
     5881 gccagcgcag ccgtaggcgt atcagattac acgtgccccc cggcctcttt tggtgctttc
     5941 cacctatcag cccgcaacca ggtcaaagga gatccacgac actggccgcc agctctcttc
     6001 cctgccgagc ctgccggccg gtaggccggc ccatcgccgg cgcagccggc gctacaaaac
     6061 gcttcggccg cttgcggccg tccgattagc cggaggggga gccgccgccg caggcggtgg
     6121 gggaaccccg caggggtgcc ccaagggggg cgacgtcgcg gagggccgca ggcccggagg
     6181 ttgtcaggat ggcagggacg aagcgggagc aaatggccct ggcgcacaag gcgcagggct
     6241 acgacgtggc cgacgtggag cggccagcga gggaacggtg ggtgacgatg agcaatgcgc
     6301 tcacgcgggc aggccacggc ctgacgctgg ccgagaaacg catcgtggcc gctgcggtgt
     6361 ccaagctggg cagccgccgc gtgctcaagc ccggcgaggt gccgcgcacc aagatcaccg
     6421 ctgccgaata tgccgagacc ttcgaggtcg atatgaacac ggcctatgac cagctccagg
     6481 gcgcggccaa gcagctctac aaccgctcga tcacgttcta cgagccagcc cacaagcgca
     6541 acggcaagcc gctgccgccg acgcgggtgc aaatgcgctg ggtggggtcg gtgaagtacc
     6601 aggacggcga gggctgggtc gagctggcgt ggtggccgga tctgctgcgc cacctcaccg
     6661 gcctgcaacg gcagttcacg acctaccagc tccagcaggc cagcgccctg cgctcgggct
     6721 actcctggaa gctcctggaa ctgctgatgc gcttcgagtc gaccggctgg gccgaataca
     6781 ccatcgagga cttcaaagcc tcgatggacg cgccaccgag cctcagtgac ttcggccaag
     6841 tgaagcgccg gatcatcgat cctgcggtca aggaactgac cgagaaggac ggctggctga
     6901 tccagtggca gccgatcaag gcgggccgca aggtcaaggc gctgcgcttc accttcatgc
     6961 gcgatccgca ggggcggctg ctgtaacaca ccgcccgcca cagcgggcaa agccgccgag
     7021 ttggacacca aagagtaagc aaaagcctac agcgggcatc gccaaaggct tacacgcgct
     7081 accgctccat gccgccgcgt gaccgctgta gggtgcgctg atgcggcgca agcaacggct
     7141 gataggtctg catcaggtct ggccgttcgc gcagctctgc ggccagcgtg gccgcctttt
     7201 ggctgctgcg accaggcata gcgttcaccg cgtccaggac tcggccaagc tcgcgcgaaa
     7261 cgccctgcac acgccccttt tggtgtgctg ccgcaaggct cacgatttgc ttggccatgc
     7321 cttgcgcggc ctgttgtagc gcacgctgct gcgcccgttc tgccgccacc tgttcacgcg
     7381 ctgccggaac ccgttgcgcc catgctggat acttccgcgc caaggtgcgc tcggccttgg
     7441 ttgtggcctt gtcgcggtag tgagtgcgcg acaaataccc ctcaagccgc ttgagacgtc
     7501 gcattaggtc ggcctctcgc cgctgcttcc agccctcgat gcgcttcatc gtgccttgcc
     7561 agtcggccag cgccttgccg tactcggcta ctttgcccgt gatccgattc cacagcgtgt
     7621 ccttcacctc ggcggggcgc ttttcagcgt ggtctacccg cgcccgatac cgcttgtcgc
     7681 ctatgtcgtg gatctccgtc cgcaagcggt gctcaaggcg gtcagcccgc tgcctgtagc
     7741 ccttggcaag ctccttggtg gtcgtatccc acttctcaac cagctcggcg gccggcatcg
     7801 cctcaaactg ccgctcggcc tccaggtgga tgacctcggc gcgggccagc tcctggcggg
     7861 cctcggccag ctcggcgcgg gcctgccgga actcgatcac gttggcccgg ccctggccgc
     7921 gccactggct cggcaactgc ttgcgctccg gctccaggcc ggtgccgcgc tcggcgtggc
     7981 tgcgcatgtc gatgcgctgc tccaccccgg cccgctccag gtgcgcattg cacatggcct
     8041 cccagcgcct gcgcagctct ttcagctcgg cggcccgctc ggccttggtc aaggtctggc
     8101 ccgcgctcgg gccgtagccc ttgcgtgcgc cgcccttctc cggggccttg gcgttgtacc
     8161 gcttgaagta ctgctccggg tcgcgctcga tgccgtcgcg ctgccgctcg gaaaacatca
     8221 ggtggacgtg cggctgctcg ccaccatcgg cggcggtcgg tacatggatg gcccactggt
     8281 aggcgtgccg gtcgcctatt tcctggcgta caaactcgcg caccagcgcg gcccgctgct
     8341 cggcgtccag ctcacgcggg agggcaatct ccatttcacg gtaggtggtg ccgttcttgc
     8401 gctcgtaggc gtcggcggcc tgccagaagg caagcgggtt ggactgcgcc gaggcgggca
     8461 tgttgcccgc ctcggtcgct tccaaccttt cgccgcgctc taggcggttg gcgtactggc
     8521 cttcgcgggc gatgtaggcg gcatgaggcc cggccttacc ggccttgcct accttcatcg
     8581 agagccgcgc tatcgccatg ccgccgcctt tcctttggtg ttgccgttgc gccgcaggcg
     8641 caggggttca ggggcttgcc cctgacgcgc gtttctcggc ttgccgagaa acaactaagc
     8701 gcgccttcta tggaaggaag catacagcgc atgcacacgc gctgtcacca tttgcataga
     8761 atggtgcatc acgaccaaag tgagtagcac cgatgacgca aaacaaatgg ctcaatgagc
     8821 ggctggcata tattcgtggc ctcaaagctc cgaatgacca tctgctgacc ttaacagttg
     8881 caggtgttgc agttcagatt tgaatccacc aagatagtcg ttgtcgagct ttggggcttt
     8941 ggggctttgg tggtaaagca ttttctaaag gctcacaaac cacggttatc caattcactg
     9001 agatacaaca tgaagtttcg agtcttttgc ttggcagcac tcccctgctt ttcggtttca
     9061 gcgcagtctt tagaaaattt agggtccttg acggccgacc aacagggagc cagctctgcc
     9121 actgccgtga gcgcggacgg cagcacggtc gtgggggagg ctgcttacga tctcacggca
     9181 ggcgggacgg gcaggcatgc atttcgctgg accagtgcgg gcggcatgca ggacttgggc
     9241 gacttgacgg ccaaccaaca gggatacagc tctgccactg ccgtgagcgc ggacggcagc
     9301 acggtcgtgg ggggggctgc ttacgatcgc acggcaggcg ggacgggcag gcatgcattt
     9361 cgctggacca gtgcgggcgg catgcaggac ttgggcgact tgacggccaa ccaacaggga
     9421 tacagctctg ccactgccgt gagcgcggac ggcagcacgg tcgtgggggg ggctgattac
     9481 gatcgcacgg caggcgggac gggcaggcat gcatttcgct ggaccagtgc gggcggcatg
     9541 caggacttgg gcgacttgac ggccgaccaa cagggagcca gctctgccta tgccgtgagc
     9601 gcggacggca gcacggtcgt ggggggggct aattacgatc gcacggcagg cgggacggac
     9661 tatcatgcat ttcgctggac cagtgcgggc ggcatgcagg acttgggcga cttgacggcc
     9721 aaccaacagg gatacagcca tgccactgcc gtgagcgcgg acggcagcac ggtcgtgggg
     9781 tgggctaatt acgatctcac ggcaggcggg acgggcaggc atgcatttcg ctggaccagt
     9841 gcgggcggca tgcaggactt gggcgacttg acggccgacc aacagggagc cagctctgcc
     9901 tatgccgtga gcgcggacgg cagcacggtc gtggggtggg ctaattacga tcgcacggca
     9961 ggcgggacgg actatcatgc atttcgctgg accagtgcgg gcggcatgca ggacttgggc
    10021 gacttgacgg cc
//
