LOCUS       NZ_AP027864             1901 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017-19-1CC plasmid p2017-19-1CC_10,
            complete sequence.
ACCESSION   NZ_AP027864
VERSION     NZ_AP027864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562630
            Assembly: GCF_036506655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 523x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036506655.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:01:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,921
            CDSs (total)                      :: 4,796
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 61 of 232
            Pseudo Genes (incomplete)         :: 163 of 232
            Pseudo Genes (internal stop)      :: 47 of 232
            Pseudo Genes (multiple problems)  :: 36 of 232
            Pseudo Genes (short protein)      :: 4 of 232
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027864.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017-19-1CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017-19-1CC_10"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(join(1841..1901,1..227))
                     /locus_tag="AABJ85_RS24595"
     CDS             complement(join(1841..1901,1..227))
                     /locus_tag="AABJ85_RS24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049092722.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(224..772)
                     /locus_tag="AABJ85_RS24600"
                     /old_locus_tag="VEE77_47400"
     CDS             complement(224..772)
                     /locus_tag="AABJ85_RS24600"
                     /old_locus_tag="VEE77_47400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_106642455.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQGDAL"
                     /name="replication/maintenance protein RepL"
     gene            1266..1391
                     /locus_tag="AABJ85_RS24605"
     CDS             1266..1391
                     /locus_tag="AABJ85_RS24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_072646109.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPLLFFQG"
                     /name="replication protein"
     Typing          1345..1605
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.618"
                     /coverage="100.0"
                     /evalue="8.2e-133"
ORIGIN
        1 ttttttggct tttgtatttt ttcatcgttg ctaacgacaa acattatttt atttccatct
       61 tcattgataa catcaacaag attaaattct ttatcttcac aattcatata aagatttatt
      121 cctgttgcat attcaatacc atatttatta attaactcat ctattgaact gcatatttct
      181 aattttagtt cttctaatct ccaacgtgat tcatatttaa ttctcatagt gcatcaccct
      241 gctctgagtc atgattagag tactttaagg aactttcaaa ctcttcacgc ctagaaagca
      301 taagccgcaa tttacgatct ataaccccat ctttttgagt taatgttttt gcgttaaatg
      361 ctgcatattt tttagtattg ttatatccac gccatacctg ataaggatct aatgcatagg
      421 cataatatat gcctactggt attttaacta ttgcgccaat actttctaaa tccccaatcc
      481 aatattggat cgttcttaaa ctaacaccga aataatcagc taagaatttt tgatcacata
      541 caacagcccc acatgattta tctatattct gggcaaaaaa agaatataac cgaaatgctc
      601 taggaacttt ttctgatata gcgtccaaca tattccaacc agccttagta acctgcgacc
      661 agtctggagg attcaaccga tctcgttttt gcttagcgtt cttggattga tcttgtacct
      721 gatgatctcg ccgtttttgc tgttcaaagt cttcaaattg ctgttccaac atacttgcat
      781 ccttacctgc aatgatattt cgcaccttac gaaatttata tcgtacagta cgaaacaaca
      841 catgcaaaca tgtttgcgta tacacatgca aataacgttt cgtatgatgg tgtcttaagt
      901 caatgatttt taaagaaatg tcaatttttg ctctaaagat ctatcaaagc gtttttttac
      961 tgctcatgat ttaacactac ctaactgctt ttggggtgtc ggggcgcagc cctgacccag
     1021 tcacgtagcg atagcggagt gtatactggc ttactcctct gcaagcctta ccagccgtga
     1081 tttttgccag atttcatgct ctcaacgttc aacaactcca tcatggtttt gccgtccgtt
     1141 caccgctaaa aagcctcaga aatcgggaca ggatgtgcat ttgtcggacc gcgcacacgc
     1201 aatcagacaa atgccacctg gtcagggctt caccccgaca ccccgcatca gtgcgtaagt
     1261 gctcaatgtg gcttccggtt ctcttccgct tcctcgtcga ttcgctcgct acgctcggtc
     1321 atcgacttgc ggcgagcggt atcacctctc gcatgttgtc ggtgatcccg ttgttgttct
     1381 ttcagggatg agtcgtcaga tagcccttca aacgcgctca gaagctctca gacgcactaa
     1441 acgggtctta aatgcccgtc agggcatggg aggcgcgaaa aacgcttaca gagcgtttca
     1501 ggaggtcagg cgcgccgata cccctaacca ggggctttca ggtcgctagg aacagcgtca
     1561 gccccctgca aagcctattt gcacggcaca ggcagctatt cggttggtag gtggtcgtag
     1621 accattggtg ttacagcgat gggggtctgg ggttctcccc agacggtttc cggttgcgct
     1681 caaggcgcta tcggaaccgt ggcggctcgg tcgttctgga gagcggggaa caaaaacacg
     1741 caagggattc gaggcggcaa aagagcaatg caggatagac aatcatggtc agaacgttaa
     1801 cacgtagccc ctgattgtcc gcctgccgct ggctcaatgg tcattttttt cgatatttat
     1861 ctagctcaat tacaggtgct ggagtttttt tctttgctat t
//
