LOCUS       NZ_AP027861             4593 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017-19-1CC plasmid p2017-19-1CC_7, complete
            sequence.
ACCESSION   NZ_AP027861
VERSION     NZ_AP027861.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562630
            Assembly: GCF_036506655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 523x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036506655.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:01:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,921
            CDSs (total)                      :: 4,796
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 61 of 232
            Pseudo Genes (incomplete)         :: 163 of 232
            Pseudo Genes (internal stop)      :: 47 of 232
            Pseudo Genes (multiple problems)  :: 36 of 232
            Pseudo Genes (short protein)      :: 4 of 232
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027861.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017-19-1CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017-19-1CC_7"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            340..900
                     /locus_tag="AABJ85_RS24515"
                     /old_locus_tag="VEE77_47310"
     CDS             340..900
                     /locus_tag="AABJ85_RS24515"
                     /old_locus_tag="VEE77_47310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            907..1140
                     /locus_tag="AABJ85_RS24520"
                     /old_locus_tag="VEE77_47320"
     CDS             907..1140
                     /locus_tag="AABJ85_RS24520"
                     /old_locus_tag="VEE77_47320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1828..2667)
                     /locus_tag="AABJ85_RS24525"
     CDS             complement(1828..2667)
                     /locus_tag="AABJ85_RS24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(2859..3008)
                     /locus_tag="AABJ85_RS24530"
     CDS             complement(2859..3008)
                     /locus_tag="AABJ85_RS24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     gene            complement(3678..3869)
                     /locus_tag="AABJ85_RS24535"
                     /old_locus_tag="VEE77_47330"
     CDS             complement(3678..3869)
                     /locus_tag="AABJ85_RS24535"
                     /old_locus_tag="VEE77_47330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3932..4255
                     /locus_tag="AABJ85_RS24540"
                     /old_locus_tag="VEE77_47340"
     CDS             3932..4255
                     /locus_tag="AABJ85_RS24540"
                     /old_locus_tag="VEE77_47340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_252312036.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQVVREQGGR
                     DDS"
                     /name="plasmid mobilization relaxosome protein MobC"
     Typing          3506..3590
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(2937..3495)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1238
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4234..4586
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.508"
                     /coverage="92.0"
                     /evalue="6.3700000000000024e-59"
ORIGIN
        1 gtaagcgcct ccagccgtac tacgaccgtg cggacaggcc gaggataaat gcctggcaga
       61 cgctggttaa tcatcattac gggctgcgtg acccgaacgc accggagaac agacggacgc
      121 ttgtcacacc gaataatctg ccaaaagcga aacaggaggc cgcagaagcg attacacggg
      181 gtcttgaggc gctttaccgt gccggagcaa taaaaacgcg tcaggatgtc actgaggcgc
      241 tcacagcggc aggttttgag gtggtgagga caaccagaag cagtatcagt attgccgacc
      301 cggagggggg gcggaatctc aggctaaagg gggcgctgta tgagcaatct tttacaaatg
      361 ggaacggact tcgagaagaa actgaaagag cgagccgctt ctaccgagaa catgctgaac
      421 agcgagttca ggaagctaga agagtctgta gacaaggcac tgagcttaaa caggcagaaa
      481 atcagagacg ctatcagcga acatacaaca tccgtgaaac agcaactgga tacgctgagc
      541 accaccgtga gcacgcagct cagcaccaca gaggccgaac tgtcccggca gcagaaaaac
      601 ctgctctggc aggtgatcaa agggcgggta ctgtttccgg cgctgaccgc gctcagcgtc
      661 acgggcggca ttttcctggg gtgctggggg ctgattcagt ggcaggaaag caggattgcg
      721 aaaaatatct tgaccatcag agagcaggaa aacacgctgg cgaaactcga ggcaaaaacc
      781 tggggggtga cgttcgtcaa tggcgagaac gggaaatttc tggtgctgcc ggacggggtg
      841 aaaggggaaa acacctggac ggtgggcgac aagaacgccg tgaggctggt cagggagtaa
      901 acgagtatga cggaactgga agggcatttg ctgaacgcct tagagcattt gcaacaggac
      961 tatatgcggc ggctgaacga gtgggagagc gccttcgcag aattgcagaa gatgcacgcg
     1021 gttacgcaac agaacaacgc gatactgaac gagcgtgtcg tgaacttgag tcagcaggtg
     1081 cagctattag ccggacaagt cgaccgcttg agtcggttat ttatcacgaa caacagataa
     1141 gggatgaaca ggagcgtcag gcgcaggagc ttgcccggcg ctggcatgaa gagcaggaac
     1201 ccgaagcgcg gatctggcgt ggcccgtcaa tggggatgtg accggggtac cccctgaaat
     1261 cgagccagtt tgtggcacgc cctacccgga tggcgccaat gttaatgtac gtacattagc
     1321 attaacaata tccaaacctc cccattttgc cccgttcgac ggctggaacg tgcgaaactg
     1381 atcgtcattc agtttcgcgc ataactatgc atgaggttaa aatttaccag gcgcgatcgc
     1441 ggcagttttt cgggtggttt gttgctgttt ttacctgtct gctgccgtga tcgcgctgaa
     1501 cgcgtttaag cggtacgcgc aatgcgacgt tatggtaaat acggagtatt ttaattgcgg
     1561 gcctttgttg tatatctctg tcgtttgtcc ccggggggaa gcattactgc taacaagtcc
     1621 cgtgagaaaa ctgcgcaatc gtattgcgca ccatgaacct attctcaacc gcaatctgga
     1681 ggatgatttt gctacgataa agagaattat cgcgtatcgg tgtcagcatt cattggagtg
     1741 gatgctgaaa aaccaggtgt tactccctct acttacactt aaacctctct aaaaatataa
     1801 gtaagtacta tcgagggcta tcatgaacta tggtgccctc tgagcatagc tacccattac
     1861 ttcttctatg tcattgaata attcatttct taactctaag tattctagta actccatttc
     1921 taatgactct gtcgtgaact taaatctaga aggatctttg attgagttta ctagtcttga
     1981 taacgctcta tatgttttga gtgaaggttg cattatagat ggaaagtatt tgaaaacctc
     2041 ttcatctctg ttgaaattaa ctaaaaactt gtagtcatca tttataaatc tcaagcatac
     2101 agcgtcaagc ttatcgcctt ttcttattat tatttcttta taggaaagat atttttcaga
     2161 agataagtcc cacatcaagc gaaaatctct ctttgcgaac gagttcgcgt catcattgaa
     2221 aagagatgat gtgatggctt tggatagacc ttctgtgttt atctctttgt tggatgggta
     2281 gtaaatttta ttaattgctt tcattctctc aaatgatgaa tataggtcat tatgccacct
     2341 tgttgaaatc actcctttag ttatattctc cttgctaaaa ttaagccctg tatcaaaaat
     2401 aaatgcagcc atcagtgaat atgatatgtt ttttattaac tcagacttaa atggttccgc
     2461 tatggagtag tacagtgata aaataactaa aaataaaagg tattgtctta cctttcccga
     2521 tgagtatatg taaaggcgat atttatttat tgatagtttt attttgtatg gaatgatctc
     2581 atttctgtca tatcctcttc gtggtggagc aaaacaaaag aaacatgcag ctattaataa
     2641 aacaaaaaag atgaacagtg taagcaagtt cggctcctgt gtatgctcac tatgcatgat
     2701 tgtcttgaga tctaacgatc ctgaaggtag tcttgtccgt aaacgaaaaa accgcccagc
     2761 aaggcggctt ttcgaaggtt ctcagagcag caactctttg aaccaaggta actggcttgg
     2821 aggagcacag ccaccaaatc tgtcctttca gtttagcctt aaccggcgca taacttcaag
     2881 actaactcct ctaaacggtt accaatggct gctgccagtg gcgtttttac gtgtctttcc
     2941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg ggccgagata ccaacagcgt
     3061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3181 tatagtcctg tcgggtttcg ccacctctga cttgagcatc tgtttttgtg atgctcgtca
     3241 ggggggcgga gcctatggaa aaacgccagc ggcgcggcct tcttcctgtt cctggccttt
     3301 tgctcacatg tttcttttgg cgttatcccc tgattctgtg gataaccgca tcaccgtctt
     3361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga
     3421 ggaagcggaa gagcgcctga tgcggcattt tctccttacg catctgtgcg gtatttcaca
     3481 ccgcatatgg tgcacttttc ttataatccg cactgatgcc acatagttaa gccagtatac
     3541 actccgctat cgctacgtga ctgggtcagg gctgcgcccc gacacccgct aaaacccgct
     3601 gacgcgccct gacgggcttg tctgctcccg gcatccgctc acaggcaagc tgtgaacgtc
     3661 tccgggagct gcatgtttca gttgctttca ccgtcatcgc cgaagcgtgc gaggcaacta
     3721 cggtagagtt cgtcggcgtg gtcgtgaagt gattcgcaga tgtctgcctg ttcgtccgcg
     3781 tccagctcgt tgagtttttc cagcagggtc agcgtctggc ttctgataaa gcgggccatg
     3841 ttaagggctg ttttttcctg tttggtcatg gtcttaaggc tccttaatag gggatttttt
     3901 agggggaatg ataccgatga aacgcgagaa gatgctcacg atacgggtta gcgatgagga
     3961 gcacgcccgg ttactggaac gctgcgaggg taaacgactg gcagaatgga tgcggcgggt
     4021 ctgtctgggg gagccggttg cgcgcacagg aaaactgccg acactatcgc cgccattgct
     4081 gcggcatctg gctgccatcg ggaacaatct gaaccagaca gcccggaagg tgaacagcgg
     4141 ccactggtct tcgattgacc gggttcatgt ggtggccgct ctgatggcca tcgagggtga
     4201 attgcgccag ttgcggcagg ttgtcaggga acagggggga cgggatgata gttaaatttc
     4261 atgccagggg gaaaggcggt ggaagtggcc cggtcgatta cctgctgggg cgggagcgta
     4321 accgcgaagg cgcaacggtg ctccggggta acccggaaga aatccgggaa ctcattgatg
     4381 ccacgccatt ttcgaagaaa tacacgtcag gcgttctgtc gttcgcggag aaggagttac
     4441 cgccgggtga acgcgaaaga gtgatgacga gctttgagcg tgttcttatg cccggtctcg
     4501 aaaaagacca gtacagcatt ctctgggtgg aacaccagga taaggggcgg ctcgaactga
     4561 attttgtcat tccgaacatg gaactacaga gcg
//
