LOCUS       NZ_AP027853             6647 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.18.04CC plasmid p2017.18.04CC_3,
            complete sequence.
ACCESSION   NZ_AP027853
VERSION     NZ_AP027853.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562610
            Assembly: GCF_036506435.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 136x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036506435.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:00:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 220
            CDSs (without protein)            :: 220
            Pseudo Genes (ambiguous residues) :: 0 of 220
            Pseudo Genes (frameshifted)       :: 66 of 220
            Pseudo Genes (incomplete)         :: 152 of 220
            Pseudo Genes (internal stop)      :: 36 of 220
            Pseudo Genes (multiple problems)  :: 34 of 220
            Pseudo Genes (short protein)      :: 3 of 220
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027853.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.18.04CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.18.04CC_3"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(join(5720..6647,1..641))
                     /gene="cea"
                     /locus_tag="AABJ74_RS25470"
     CDS             complement(join(5720..6647,1..641))
                     /gene="cea"
                     /locus_tag="AABJ74_RS25470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            901..1338
                     /locus_tag="AABJ74_RS25475"
                     /old_locus_tag="VEE64_48770"
     CDS             901..1338
                     /locus_tag="AABJ74_RS25475"
                     /old_locus_tag="VEE64_48770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1419..1838
                     /locus_tag="AABJ74_RS25480"
                     /old_locus_tag="VEE64_48780"
     CDS             1419..1838
                     /locus_tag="AABJ74_RS25480"
                     /old_locus_tag="VEE64_48780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2156..2389)
                     /locus_tag="AABJ74_RS25485"
                     /old_locus_tag="VEE64_48790"
     CDS             complement(2156..2389)
                     /locus_tag="AABJ74_RS25485"
                     /old_locus_tag="VEE64_48790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2396..2914)
                     /locus_tag="AABJ74_RS25490"
                     /old_locus_tag="VEE64_48800"
     CDS             complement(2396..2914)
                     /locus_tag="AABJ74_RS25490"
                     /old_locus_tag="VEE64_48800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2952..3650)
                     /locus_tag="AABJ74_RS25495"
                     /pseudo=""
     CDS             complement(<2952..3650)
                     /locus_tag="AABJ74_RS25495"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3592..3915)
                     /locus_tag="AABJ74_RS25500"
                     /old_locus_tag="VEE64_48810"
     CDS             complement(3592..3915)
                     /locus_tag="AABJ74_RS25500"
                     /old_locus_tag="VEE64_48810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3979..4170
                     /locus_tag="AABJ74_RS25505"
                     /old_locus_tag="VEE64_48820"
     CDS             3979..4170
                     /locus_tag="AABJ74_RS25505"
                     /old_locus_tag="VEE64_48820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4840..4998
                     /locus_tag="AABJ74_RS25510"
     CDS             4840..4998
                     /locus_tag="AABJ74_RS25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5197..5334)
                     /locus_tag="AABJ74_RS25515"
     CDS             complement(5197..5334)
                     /locus_tag="AABJ74_RS25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5496..5723
                     /locus_tag="AABJ74_RS25520"
                     /old_locus_tag="VEE64_48830"
     CDS             5496..5723
                     /locus_tag="AABJ74_RS25520"
                     /old_locus_tag="VEE64_48830"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4256..4343)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4354..4912
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2053..3602)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcttaatct ctccatccat tttcaccact tcagattgtg cagcagaaag ttttttttgg
       61 gcgatctcaa cagctttagc ttcttcactc aatgcagcca gtcgtttctc ttcagcttca
      121 gccagtttca actggcgttc tgtttcagcc ttctcccgtt caatctcttt acgtcgttgt
      181 tctgcttcct gaaaagcctt ttctgctgct tccgcttctt tacgggcttt ttcttctgct
      241 ttcgcaaggc gcaaacgctc tgcttccgcc tgcatagctg cattattagc atgagcaagc
      301 tctgttgctg aaggcgtacg tgaggcattg tgacgaagag cctcattcac gatatccttc
      361 aggcgctgag tcagcgcatc cctgtttgcc tttgctttcg cctgtgcttc cgctgcagct
      421 tttgcccggg cagcctgctc tgcctgtgtt ttctttaatt gagcagtaga ccatttagca
      481 gttgcatgaa tagctgcaga actttcactt ttactgcctc cttttccacc tccgccgcca
      541 gagccactcc cgtcaggagt accattcaaa agagtaataa ttacctgtcc cttatcatca
      601 taaggaacac catctttata gtacgctacc gcggtttcca ttataaaatc ctctttgact
      661 tttaaaacaa taagttaaaa ataaatactg tacatataac cactggtttt atatacagca
      721 taaaagctac gccgctgcat tttccctgtc aagactgtgg acttccattt ttgtgaaaac
      781 gatcaaaaaa acagtctttc acaccacgcg ctattctcgc ccgatgccac aaaaaccagc
      841 acaaacatta ccgttctcag acctcattat gttttactga aactatgaga tgagacatct
      901 atgggacact gtcactttat ggcatggcac acactccggg acgcactaaa aatgacaggc
      961 agatcgcgtt cacagtttta ccgtgatatg cgcgcaggcc ttgtcagtta ccgtaccggc
     1021 agggacggac gacgggagtt tgaaaccagt gaactgatcc gggcatacgg cgaattaaag
     1081 cagaatgaga caccagaaag gcacagtgag ggacatgcag aaaatccaca tgatcagcag
     1141 acagaacgca ttctccggga gctgaatgag ctgaaacaat gcctgacgct gatgcttgag
     1201 gataaacagg cacaggatat ggatcgcaga cgccaggaag cagaacggga acagctacaa
     1261 aatgagatag cccagctcag gcaggcactg gaactggaaa agaaacgggg attctggtcc
     1321 aggttgttcg gtcgctgaac gctgtcagag actgatgata aaatagtctt cggataataa
     1381 ctcaccgaga ataaatactt taaggtaggg agacactcat gagacgtacc ggaaacaaac
     1441 tttgtcttat cgccatgata acagcaacag tagctctcac agcctgtacc ccaaagggca
     1501 gcgtggaaca acatacccgg cattacgtat atgcttctga tgacggtttt gatcccaact
     1561 tttccaccca aaaagccgac acaacacgaa tgatggtgcc tttttttcgg cagttctggg
     1621 atatgggagc taaagataaa gcgacaggaa aatcacggag tgatgtgcaa caacgcattc
     1681 agcagtttca cagccaggaa tttttaaact cactccgggg cacaactcaa tttgcgggta
     1741 ctgattaccg cagcaaagac cttaccccga aaaaatccag gctgctggct gacacgattt
     1801 ctgcggttta tctcgatggc tacgagggca gacagtaagt ggatttacca taatccctta
     1861 attgtacgca ccgctaaaac gcgttcagcg cgatcacggc agcagacagg taaaaatggc
     1921 aacaaaccac ccgaaaaact gccgcgatcg cgcctgataa attttaaccg tatgaatacc
     1981 tatgcaacca gagggtacag gccacattac ccccacttaa tccactgaag ctgccatttt
     2041 tcatggtttc accatcccag cgaagggcca tccagcgtgc gttcctgtat ttccggctga
     2101 cgctcccgtt ccagggataa cacatgttcg cgctcctgta tcagccgttc ctctcttatc
     2161 tccagttctc gctgtataac tggctcaagc gttctgtctg ctcgctcaag tgctgcacct
     2221 gctgactcaa ctgcatgacc cgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa
     2281 acatcttctg caattccacg aaggcgctct cccattcgct cagccgctgc atatagtcct
     2341 gttgcagctg ctctaaggcg ttcagcaaat gtgtttccag ctctgtcact ctgtgtcact
     2401 ccttcagatg tacccactct ttcccctgaa agggaatcac ctccgctgat ttcccgtacg
     2461 gaaggacaag gaatttcctg ttcccgtcct gcacaaactc cacgccccat gtcttcgcgt
     2521 tcagtttctg caatgtctct tcctgcttcc tgatttcttc caggttcgcc tgtatcctcc
     2581 ctccaagata ccagagcgtc ccgccactcg cggtaaacag gagaaatact atccccagta
     2641 acatcatgcc cgtattccct gccagcttta acacgtccct cctgtgctgc atcatcgcct
     2701 ctttcacccc ttcccggtgt ttttccagcg attcctctgt cgaggctgtg aacagggcta
     2761 tagcgtctct gattttcgtc tcgtttgatg tcacagcctc gcttacagat tcgccgagcc
     2821 tcctgaactc gttgttcagc attttctctg tagattcggc tctctctttc agctttttct
     2881 cgaactccgc gcccgtctgc aaaagattgc tcataaaatg ctcctttcag cctgatattc
     2941 ttcccgccgt tcggatctgc aatgctgata ctgcttcgcg tcaccctgac cacttccagc
     3001 cccgcctcag tgagcgcctg aatcacatcc tgacggcctt ttatctctcc ggcatggtaa
     3061 agtgcatcta tacctcgcgt gacgccctca gcaagcgcct gtttcgtttc aggcaggtta
     3121 tcagggagtg tcagcgtcct gcggttctcc ggggcgttcg ggtcatgcag cccgtaatgg
     3181 tgattcacca gcgtctgcca ggcatcaatt ctaggcctgt ctgcgcggtc gtagtacggc
     3241 tggaggcgtt ttccggtctg tagctccatg ttcggaatga caaaattcag ctcaagccgt
     3301 cccttgtcct ggtgctccac ccacaggatg ctgtactgat ttttttcgag accgggcatc
     3361 agtacacgct caaagctcgc catcactttt tcacgtcctc ccggcggcag ctccttctcc
     3421 gcgaacgaca gaacaccgga cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc
     3481 cggacttctt ccggattacc ctgaagcacc gttgcgcctt cgcggttacg ctccctcccc
     3541 agcaggtaat caaccggacc actgccacca ccttttcccc tggcatgaaa tttaactatc
     3601 atcccgcgcc ccctgttccc tgacggccag acgcagccgg cgcagctcat ccccgatggc
     3661 catcagtgcg gccaccacct gaacccggtc accggaagac cactgcccgc tgttcacctt
     3721 acgggctgtc tgattcaggt tatttccgat ggcggccagc tgacgcagta acggcggtgc
     3781 cagtgtcggc agttttccgg aacgggcaac cggctccccc aggcagaccc gccgcatcca
     3841 taccgccagt tgtttaccct cacagcgttc aagtaaccgg gcatgttcat catcagtaac
     3901 ccgtattgtg agcatcctct cgcgtttcat cggtatcatt accccatgaa cagaaatccc
     3961 ccttacacgg aggcatcagt gactaaacag gaaaaaaccg cccttaacat ggcccgcttt
     4021 atcagaagcc agacattaac gctgctggag aagctcaacg aactggacgc agatgaacag
     4081 gccgatattt gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ctgcctcgca
     4141 cgtttcgggg atgacggtga aaacctctga tacatgcagc tcccggagac ggtcacagct
     4201 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc
     4261 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4321 accatgcggc atcagtgcgg attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc
     4381 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     4441 cgctacgctc ggtcgttcga ctgcggcgag cggtactgac tcacacaaaa acggtaacac
     4501 agttatccac agaatcaggg gataaggccg gaaagaacat gtgagcaaaa gaccaggaac
     4561 aggaagaagg ccacgtagca ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4621 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4681 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4741 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat
     4801 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4861 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aaggcacgcc
     4921 ttaacgccac tggcagcagc cactggtaac cggattagca gagcgatgat ggcacaaacg
     4981 gtgctacaga gttcttgaag tagtggcccg actacggcta cactagaagg acagtatttg
     5041 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5101 gcaaacaaac caccgttggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5161 gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag
     5221 tttagaagag gaggatggtg cgatggtccc tccctgaaca tcacgtatat agttagcctg
     5281 acatccaaca aggaggttta tcgcgaatat tcccacaaaa aatcttttcc tcataactcg
     5341 atccttataa aatgaaaaga atatatggcg aggtttaatt tatgagctta agatactaca
     5401 taaaaaatat tttatttggc ctgtactgca cacttatata tatatacctt ataacaaaaa
     5461 acagcgaagg gtattatttc cttgtgtcag ataagatgct atatgcaata gtgataagca
     5521 ctattctatg tccatattca aaatatgcta ttgaacacat agcttttaac ttcataaaga
     5581 aagatttttt cgaaagaaga aaaaacctaa ataacgcccc cgtagcaaaa ttaaacctat
     5641 ttatgctata taatctactt tgtttggtcc tagcaatccc atttggattg ctaggacttt
     5701 ttatatcaat aaagaataat taaatcccta acacctcatt tatagtatta agtttattct
     5761 tatcaatata ggagcataga attcctgtaa caatagcaat accccaaata cctaatgtag
     5821 ttccagcaag caagctaaaa agtaaagcaa caacataact cacccctgca tctgcagctt
     5881 tcttctctaa tgtaagaaat agtggcttcc agtcacctgt atccttaatt tttaggatat
     5941 cagataccac atcatatcca aaagaaacat gccccgtaat cttcaagtac ttggcaaact
     6001 gatctaaatg tttagcccag tcatcatact tcaccgatgc caacgcatta aaaatagcat
     6061 cacgatcggc tttgctgaat ttcttattta aaacatcctt gtatttttca aaagcagcga
     6121 gagcttcatt cacattgccg attttcttac ctttagactt atcagcaagt tcctgtgcca
     6181 ttttcgaata tttttcacca tatttttcag tcagcgtttg ataaaagcta actgttgcat
     6241 caacagcatc cttaatctgt gaattaagga gattattctg tgcttttttc aaattttctt
     6301 cagcttcatg aacacgagcg ataccggcat tacgattatt actgacctga gaaatagcct
     6361 tctggatctg agttatatca gcatttatcc ggttaatacg tgtttctgat gctgttacct
     6421 gtttttgttt ttcttctcta atcttaccgg ccccaacccg tcgtctggtt gcttcaaaaa
     6481 aaggacggtt ctgaagcgga tcattggctc ttggtgatag ttttttgacc agctcatcca
     6541 gttctttata tttagcggat gcctgagcca gttcatttcg ttttccagcg agcgttttca
     6601 tttctgcatc acgggcatgg atactggagc ttaaacgaga attgaga
//
