LOCUS       NZ_AP027846             2443 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.18.02CC plasmid p2017.18.02CC_2,
            complete sequence.
ACCESSION   NZ_AP027846
VERSION     NZ_AP027846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562607
            Assembly: GCF_036506055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 118x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036506055.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:15:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,752
            CDSs (total)                      :: 4,634
            Genes (coding)                    :: 4,439
            CDSs (with protein)               :: 4,439
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 71 of 195
            Pseudo Genes (incomplete)         :: 120 of 195
            Pseudo Genes (internal stop)      :: 43 of 195
            Pseudo Genes (multiple problems)  :: 37 of 195
            Pseudo Genes (short protein)      :: 2 of 195
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027846.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.18.02CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.18.02CC_2"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            join(1877..2443,1..402)
                     /gene="mobV"
                     /locus_tag="AABK09_RS23750"
     CDS             join(1877..2443,1..402)
                     /gene="mobV"
                     /locus_tag="AABK09_RS23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(472..702)
                     /locus_tag="AABK09_RS23755"
                     /old_locus_tag="VEE62_45630"
     CDS             complement(472..702)
                     /locus_tag="AABK09_RS23755"
                     /old_locus_tag="VEE62_45630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152923862.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNVAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(689..1171)
                     /locus_tag="AABK09_RS23760"
                     /old_locus_tag="VEE62_45640"
     CDS             complement(689..1171)
                     /locus_tag="AABK09_RS23760"
                     /old_locus_tag="VEE62_45640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          2..399
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.241"
                     /coverage="100.0"
                     /evalue="8.07e-58"
ORIGIN
        1 cgtggcaccc gtagccaggt atcgatctcg ccggaggcgc tggagccgcg tgtattgcgg
       61 aagggcattt tcaccaaaga cgtggaagat caggcagcca tagccaaacg tctaagccac
      121 gcagtgaacg atggctttgc aggaaccatc gctatggcct ctcagagcgc ccagaacgca
      181 aaaagagcgc gtgagctaca gaagactatg gatgcgcagc agaaacgcct acagagcgtc
      241 acagagcctt ttaaggggct ttccagagag cagatgaccg aaatcctgat gatggctcag
      301 cggtttaagc agcaaaacca ggaaaaggaa aaacagcagc gtattgaacg tgaaaaacag
      361 cgacagacgc gatcgcgtgg catgggtggt atggagcgtt agttttgatg cgatgcagga
      421 tagggctaac gccgcaaagt gacgtcattt gcggcattca ccacgaaaaa attaatcatt
      481 cagatggtaa ttatgttttt ttaaaataga aaaagctacg tttttctgct ttggtgacaa
      541 acccttagtt gagtatttat taccgctagt tctacgatat atgctgtaaa cactgcttaa
      601 aaaagtacgc tcccaaacag tcagaacctc attctttttt agtgaatcat gcagcattag
      661 cctgtctaca tttttaaaaa gtttatcttc agcattcatc atccattacc ttcgtctgca
      721 ccgttcttcc attggtgtca taactgatct ttaactcaat tttctttctt ctttcgtaaa
      781 ttgatggcca gtctccccgt gcaaaaagaa aggggttagc ttcaagttcc ccacgccctg
      841 cccggaagaa tatttgttta tctacaagag ccgcaattat gtttttaaag gtctgatatt
      901 caatatgaac cctgggctgt atggtttttt caaatctagt ttttgataca gacacaaacc
      961 catcccatcc aatcagcatt gccaattgta aaagcacatc attttgagat tttgataaac
     1021 catgaatcat cgcaatgtct gcaatgtata gcttgacgta gggaggttcg gctgggatgg
     1081 aaaaggtctc gatcttagag tcttgtttta cctcaccgtt ttcatccatg tatgtatgcc
     1141 gttcaagcct agaaatcttt ttcgtattca ctccagcccc acgtttcgcc atgatttagt
     1201 actcgcattt aagtactaaa gagacataca gtcaagtcct ttgtatcaag tacgattagt
     1261 acttgaggtg agtacgatta gtccttgagg tgagtacgat tagtacttga ggtgagtacg
     1321 attggtactt gaggtgagta cgattcttcg ctctagccct tggtacaagc gggttcccat
     1381 atctatatct atatattaag ggggaaaata agggctgaaa aacggcattt ttagacactg
     1441 aaaaactgcc tttttttagc aaggagtgta cgcatttcta tgtacgggga gtggaaattc
     1501 cgcttcctcg ctttggctgt tcgttcctcg tcgcgttgct cctgcggtac gcctcagcca
     1561 aagctgcggc gagcggttcc tgctctttca aagacgattt cattgcctga tttcgtcaag
     1621 ggcgagcgta gcgagtgcca tttacccttg acgacactcc ggataaaaca ggcttggcag
     1681 tggtgggggg tggcgcgaag cgacgccttc cacctgccag accgccgttg attttgacct
     1741 tctggcagct tgctgcccga acgattttgc gtagcaaagt ctagtgagta cactatcatc
     1801 ttgatagtgg cccgccggag gtatcgcaat gcgctccccc gcctggctga atgaatgccg
     1861 gaatcagggg gggaacatgg cgaactacgc gataatgcgc tgcaaaaagc tgacgggaat
     1921 gggaagcgtg gccagcgcgt tgcagcactg ctaccgagag cgtgagacgc cgaacgctaa
     1981 cgctgaacgg acaccagaaa actactgctc tgtgtccaaa tcaacggatc aggcaatggg
     2041 gcgtgtgcgg gagctgctgc cggaaaaacg ccgtaaagat gcggtactgg ccgttgaata
     2101 cgttatgaca gcaagccctg agtggtggaa agaggccaca ccacggcagc aggcggagtt
     2161 tttcgcccgc tctgagcaat ggctggagaa gaaatacggg aaggatcgtg ttgtggcggc
     2221 ggtggttcac cgggacgagg ccaccccgca cctgtctgcg ttcgtggtgc cattgacgca
     2281 ggatggacgg ctgtccgcga aagagtttat tggcgggcgg tcaaagatgc gtgaagatca
     2341 gagcacctac gctgaatcag taaaaaaact gggtcttgag cgcggcatcg agggaagccg
     2401 cgccacgcat cagacagttc agcactacta cgaatcgata aac
//
