LOCUS       NZ_AP027842             2101 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.17.03CC plasmid p2017.17.03CC_13,
            complete sequence.
ACCESSION   NZ_AP027842
VERSION     NZ_AP027842.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562605
            Assembly: GCF_036505855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 115x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036505855.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:46:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,606
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,110
            CDSs (with protein)               :: 5,110
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 372
            CDSs (without protein)            :: 372
            Pseudo Genes (ambiguous residues) :: 0 of 372
            Pseudo Genes (frameshifted)       :: 109 of 372
            Pseudo Genes (incomplete)         :: 271 of 372
            Pseudo Genes (internal stop)      :: 48 of 372
            Pseudo Genes (multiple problems)  :: 54 of 372
            Pseudo Genes (short protein)      :: 4 of 372
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027842.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.17.03CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.17.03CC_13"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(613..879)
                     /locus_tag="AABK32_RS28015"
                     /old_locus_tag="VEE61_54360"
     CDS             complement(613..879)
                     /locus_tag="AABK32_RS28015"
                     /old_locus_tag="VEE61_54360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(883..1848)
                     /locus_tag="AABK32_RS28020"
                     /old_locus_tag="VEE61_54370"
     CDS             complement(883..1848)
                     /locus_tag="AABK32_RS28020"
                     /old_locus_tag="VEE61_54370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1224..1455)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ccttaagtat aaatacaagg atcacgcgcg agaattgcgc aaaaaataac cacaatttta
       61 accccccaaa acaagcaccc ccgaacggct taccccaacc ttttccacac acccgatcac
      121 caaaacaggg aataaaaaca aaaccctgtc ttacaggtgg atggcctcac ttcgtgaaac
      181 catccacccc gcataagttt tttaatcggt ggtgtcgtca atcactaaat cggaacccca
      241 aaggggaccc ccacgattta gcatttgacg agattaactc tcggagatgg catcatgctt
      301 ttaacggact ttaaaaaacg gtgaacggat gcggatttta atccgtgtct gtgaggcgtt
      361 gagcaacgag caaagcgagg cgatagaaag agagccagcg gcaggctcag gacgataatc
      421 cggggaaaat gtaccactcg cagagtgtta acatttgaca tcggattgtc tatcctgcat
      481 tggtgtcgag ttggcaaggc tgaggataat gctgaggata atgctgagga taatgcagga
      541 tagacagagc actagagatt gcatgcaatc tcgtacctat cacctcagcc tgccgctggc
      601 tcttttccag aactaagaat gcgttcttaa aaatccaaac ttgataccgt ttaaatctat
      661 tttcacaaaa agagttttta ccctctcgtt tgttttctga atatataaaa ttaattcatc
      721 ctcaaaacct tcgtgcggat gaaaaccaaa tgtgccaaaa cttaatccat caataagcaa
      781 aataaggttt ttaaacctgt caggaatcac cgcttcactt cttaaaaaaa ataaaccaat
      841 agtcttattt ccattcaggt attcaatatt aatattcata cgttaatccg atttatcctt
      901 ctcaggggcg cgtttgtaac gccgtttacc tgaatcccag acaaacgccg tccgcttccc
      961 gtcatcagtg ccatccccta catcgtcggc aatgacaaga tcctcattgg tttctcgatc
     1021 caactggagg acgtttttta gcgccccacc ggtcgagata aaacggcgct tgtgaagctg
     1081 ccgcgtcagc tcaagaaacc actcaggatc gtttgccata tcttccggtt taacggagta
     1141 tttcagcgtt tcagcaaccg cgctttgcag ttgctcggca acgttggcca caacctcacc
     1201 tgtcttagtt tttactgccc gaatatcgat attcggctca tagttcaccc gcaagcaatc
     1261 gcgccagagt tctacccaac gttcgtgctt aacgtagttc ttccctttaa accaagaagg
     1321 ttgcaccatc agcagacagt gaaaatgcgg atgtgcgctg ccatccttac ctcgcgtcac
     1381 ctccgtagcc ctgatccacc cctgaacagg tgatagctcc tttcgctttt ccattcgctt
     1441 aaacgccgca ttcattgctg taaggactgt tccaagttca cctatctcgc agttcctgac
     1501 agttaacgtc agaaacaacc atcgggaaga cgggtaatcc acaacgattt tcggtagagc
     1561 ctgataaaaa cgggcttgcc acatgagggt tcttctccac tggcagacag ggcaatgacg
     1621 gacacggcaa aattgcgcac tacgaagccg taggcgcgtt tctccggttt ccatgatggt
     1681 agaccaacca aaacgtaaca aaccgctaca ggaggccata cggagtgcat agcgctcgaa
     1741 ttcagcagca gtctggtaga tacccccgac agaatcggta agcgtgcgct gggtatccca
     1801 aactgcatca cgggggctgt agtccgaaag gaatttatct tctgacatta agcctgctcc
     1861 gatctgggca ggctgtgatt tgatgtggta tattcatcac gtacctgtgc attgcgagcc
     1921 agtacatgtc accagagttt gccgctgagg tgacacattc gaaatgttgg ggagtgttga
     1981 cgcactcccc aacgccgatt aaaaacgcct caattctgct ccattttctt tgacctgtca
     2041 aaacccactc aaagcctcgt cattactggg ctaaaccact atttcatatc cgccatttat
     2101 c
//
