LOCUS       NZ_AP027841             3141 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.17.03CC plasmid p2017.17.03CC_12,
            complete sequence.
ACCESSION   NZ_AP027841
VERSION     NZ_AP027841.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562605
            Assembly: GCF_036505855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 115x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036505855.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:46:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,606
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,110
            CDSs (with protein)               :: 5,110
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 372
            CDSs (without protein)            :: 372
            Pseudo Genes (ambiguous residues) :: 0 of 372
            Pseudo Genes (frameshifted)       :: 109 of 372
            Pseudo Genes (incomplete)         :: 271 of 372
            Pseudo Genes (internal stop)      :: 48 of 372
            Pseudo Genes (multiple problems)  :: 54 of 372
            Pseudo Genes (short protein)      :: 4 of 372
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027841.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.17.03CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.17.03CC_12"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(587..799)
                     /locus_tag="AABK32_RS27995"
     CDS             complement(587..799)
                     /locus_tag="AABK32_RS27995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338389721.1"
                     /translation="MRKFAILTRSTAGASETNRVPYLCIAMQHPENAFHAFLGIQPGEN
                     MVFLLFERKDVKNLVIALAIFRRRA"
                     /name="hypothetical protein"
     gene            complement(866..1399)
                     /locus_tag="AABK32_RS28000"
                     /old_locus_tag="VEE61_54330"
     CDS             complement(866..1399)
                     /locus_tag="AABK32_RS28000"
                     /old_locus_tag="VEE61_54330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106509525.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPATGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1402..2328)
                     /locus_tag="AABK32_RS28005"
                     /old_locus_tag="VEE61_54340"
     CDS             complement(1402..2328)
                     /locus_tag="AABK32_RS28005"
                     /old_locus_tag="VEE61_54340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001314449.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2504..2899
                     /locus_tag="AABK32_RS28010"
                     /old_locus_tag="VEE61_54350"
     CDS             2504..2899
                     /locus_tag="AABK32_RS28010"
                     /old_locus_tag="VEE61_54350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     Typing          complement(1406..2328)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catgggatcg tgcgatatgc tggatggtgg aaggtcggag ggaacggcaa caactcaccc
       61 ctcccagtga agggacgggt agtttgccgc tctcgatatg ccggacggag ccaaggccat
      121 cgatcacggg gcgcgtcagt ggaagaaccc gcgcctgcgt gacgtatgcg tcttcgccgc
      181 accatctcac ccgtcacgcc accacatacc gggaactgcc gccaaggtct aactcaacca
      241 atctagccgc gctacatacg gtttttgtgg caactcatca ggtgtccgct gtcgatgaaa
      301 ctctctgcgc cggtagcgga actctcacgc gtgcggtcgc cgtcgtcaca gctcccccaa
      361 ccgcaaacga ttctcgcaaa tacgcaagta tctgcaaaaa caggtttttt tcggggttcc
      421 tgggagactg tgctgttgaa aaattcttga agaatacaga aggctcatgc aaactgaatg
      481 aactggtttc tgtcgctcct aacaacagac tccacaggaa cattgaggag gtcagagacc
      541 tcaatgaacc cgaaacagct cgccttcctg gcgagctgtt ttcgttctac gcccgccgac
      601 gaaagatcgc aagtgcaata acgagatttt tcacgtcttt acgctcaaaa agtaaaaaaa
      661 ccatgttttc ccctggctga atccccaaaa acgcgtgaaa tgcgttttca ggatgttgca
      721 tagctatgca caaatacggc acacgatttg tttcgctcgc cccagccgtc gaacgggtca
      781 aaatggcaaa ctttctcatc agaaaacggt gaacgggacg cggaattttt tcgcgtcccg
      841 tgaggcgttg gacaagcgaa gcgcgtcagt ttttccggct cttccctgtt gctggtttct
      901 ccgccagttt atcttccagt gtcacaactt tctgatacac cgccgctgtg gcgttgtagt
      961 tcctgtcgag cattgttttc attccccatc caagcacaca gaacataacc agaaaactga
     1021 ccgtaacacc gataaaccat ttcagactgt atttattgag cgtgttgtat tctttaacaa
     1081 cattgtgatg aattgtcgca atgtccttgt tcagggcatc cgcgtttgcc ttcatgtttg
     1141 cgactgactc ttccagccgc tgaaaatcct ggctgatgcg ctcacctatc agccctgcaa
     1201 catccagctt ctgcacctcg tttctgactt catccctgac ctgttttgcc acaccgtcag
     1261 aaatatcccg atatccgcga agtgtttccc ttgccacagc cagagaatca gcaacagctt
     1321 tctgttgctc cattgcggta tgttgcagat gctccaccga taacaggagc gtgagtaatt
     1381 tttcttctgt gctggccatg ttcagaatcc catagaaaat ccacgtgacg gtgatttaac
     1441 ccgttcaggt tcagttcggt tctgtctgct cactctgtct ctcatgtcaa agccatgctc
     1501 ccgcgcaaca tcatgttgca gccttctgac cagttcatgt tgcttaaccg gagtgtccag
     1561 ccattccctg atgtcatgcc cgaccgttcg cgcctgttca agatggcggg aatagtccgg
     1621 ttcagaccgt ttttgcatga tgtcatgacg ctgggcatca tcaagccgtc cggcagcttc
     1681 aaaacccaga tgcacttcgg gacgtcgttc aatgccctgt tctttcagcg tccggcagtc
     1741 aatgaagccc ctttcaaagc catgctttct catatgttcg ttgtgcttca cctcccagcg
     1801 ttttcgcaat tcccgcaact gctctttttg ctgagtgaga gtcaggcttc cggaatcctt
     1861 tttcgcacct ccccgctcgg gatacctggc gttatagcgc cggaaatact gttccggtgt
     1921 acgttcaatg ccatcattca cacgctggct catcatgata tgcgcatgag gctgttcgcc
     1981 gccgtcaatg ctcgctttcg gattgtgaat ggcaaaactc caggcgtgcc gttcgccagc
     2041 ttcctgacgt acaaaatcct gcaccagttc aaggcgctgt tccgctgtca gttcacgggg
     2101 taatgcaatt tcaagttccc gataggtcga accgttcgca cgttcaaact ggtcagccgc
     2161 ctgccagaaa tgtgagggat tatctcgcgc ccattccggc atgtttccgt gtgcggaata
     2221 ttccaaatcc tgacggtcgc ggtatttctc tttgcgctcg atgtaatcgg cgtggtttgc
     2281 agcctgcccc ttgccaccaa atttaacgct gaggtgatag gttgccacgg tttcagccct
     2341 ccgcaggagc gcactgtttc tcgacgaagg agagaaacat ataagtgcgc ttccttctaa
     2401 ttttacgaat tagcacggaa gataccccct gcacccaccg accgcaagcg atatactgca
     2461 caaccccaac acaccgcaaa aaacaccagg gagcatccca aacatgcgca tcagcaacat
     2521 cgactggttg aagaaacgaa tcggctttat caggaagctc ggagaacaga ccgcacgaca
     2581 gaggcagatc attgacctgc tcgataacga agccggacta acagaacagg agcgcaaact
     2641 actccacgtc ctggcaaccg cagaaaaaaa cgagctacag gcacaggaga acgcacgcaa
     2701 acaggccaac caaaaacgga tggaggggaa aacacaacga cgggaacgca atcaccgcct
     2761 gtttcttgcg gcgggattac tgatcgaggc cggactagtg gacacaaaaa caggcgaact
     2821 gagataccca aaagacaata tattacagaa acttaaggca atcaggctcg atcttgaaac
     2881 aagcccagat catcattaat tccgctgcgt ggtggtgcgt ttgcctgccg gtgttcaatg
     2941 ttttcgattt cgcgacgcgc tacgcgcgga tctggcacag gcgcgtaagt gcacaatgct
     3001 tgcctctgtg actcttccgc ttcctcgccc actcgctcac tacgttcggt cgctggggct
     3061 gcggcgagcg gtgggatcac tctcgttgtg atgcagatcc gttttttttt ttgcactgga
     3121 gttatcagga gtagcggtgc a
//
