LOCUS       NZ_AP027839             4096 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.17.03CC plasmid p2017.17.03CC_10,
            complete sequence.
ACCESSION   NZ_AP027839
VERSION     NZ_AP027839.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562605
            Assembly: GCF_036505855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 115x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036505855.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:46:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,606
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,110
            CDSs (with protein)               :: 5,110
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 372
            CDSs (without protein)            :: 372
            Pseudo Genes (ambiguous residues) :: 0 of 372
            Pseudo Genes (frameshifted)       :: 109 of 372
            Pseudo Genes (incomplete)         :: 271 of 372
            Pseudo Genes (internal stop)      :: 48 of 372
            Pseudo Genes (multiple problems)  :: 54 of 372
            Pseudo Genes (short protein)      :: 4 of 372
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027839.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.17.03CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.17.03CC_10"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(214..2373)
                     /gene="mobQ"
                     /locus_tag="AABK32_RS27965"
                     /old_locus_tag="VEE61_54260"
     CDS             complement(214..2373)
                     /gene="mobQ"
                     /locus_tag="AABK32_RS27965"
                     /old_locus_tag="VEE61_54260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023142279.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLATL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            2561..2815
                     /gene="mobC"
                     /locus_tag="AABK32_RS27970"
                     /old_locus_tag="VEE61_54270"
     CDS             2561..2815
                     /gene="mobC"
                     /locus_tag="AABK32_RS27970"
                     /old_locus_tag="VEE61_54270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            3347..4054
                     /locus_tag="AABK32_RS27975"
                     /old_locus_tag="VEE61_54280"
     CDS             3347..4054
                     /locus_tag="AABK32_RS27975"
                     /old_locus_tag="VEE61_54280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(2016..2374)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2375..2554
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.238"
                     /coverage="100.0"
                     /evalue="2.68e-81"
     Typing          3348..4054
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(218..2460)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.599"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgtgttag tagtgcgaag gtactattga agtgaaaaat tgcgtcaaaa gcgctctaat
       61 gcagcgaaaa aagaaccccc atttttcggg ggcggaaaaa caaaactatg cttgcattat
      121 gtgtgtgctt cttgcattca tccctgtcga tgaacatcga agaacatcga cgaacataac
      181 acgatgttaa acaaaacaaa atattgttca cattcactgt gaccaaccgc gatcatgtct
      241 cctctcggtg gcgtgtgttc gtgcatcttc ctcgcgccgc cattcgtccc aggtacgatt
      301 aaaacccagc gagcggaaca gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa
      361 aagcggtgcc tcacgggttt tcagatgaaa ctccataagt tccggcgcag gtgatatgtg
      421 gctgtctgca tcgtgattta cggcaacacc ataccctgca taccgtaacc ccttatcggt
      481 cagtgcactg atgaccagct ggctgttatc ggctgaggga acggcaaacg cgggatacag
      541 cgtaccatta agccggacaa caccgcccag agagtcatcc acgcccatga ccagcaaaag
      601 ctgaagctgg cgggtttccc ggtcgacaga actcattacc ggcatcagcg tcgctagcag
      661 acgtccctgc gtaaaatcca ccttccttgc tgccagtgat ggcatggctg gctggcggtc
      721 ttctccaaga cagaaagact ccggccatgt cttcggctgg cggagattgc gggtatattg
      781 cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag gtcgcagagg aataaacacg
      841 ttgcagtggt ggatgttgac cgatttcgct tcggtcacag atataaccac tcctgtcacg
      901 cattaaatca acacgctcaa agattctggt gcggtatttc tccagctcgt tcccccagag
      961 cgtgtattct ccgttccggt tgtgaagcag aaggtaacag gtcatttccc cgttaccagg
     1021 catgggggcc tcgccatact ccgtaacgac tgcgctcagt acccctgaat ccataacggc
     1081 attgtcattt ttcctgagct gttccgctcg tcgctccgcg cgactacgtt ctgtatcctc
     1141 ctgctccttc cggcgcaatt cctcttcacg tttaagcaac tggactctga gagctgcaag
     1201 gccggatgcc ccccactgct gccactgttt ttcaaatgcc gtctgactcg cgggaatgtc
     1261 ctgtttctcc accttccggt aatgctgtcg caatttttcc atttgctggc tcagcgactg
     1321 gatttttccc tgttcgctac ggtattccgg tgccgcccat tgcatcaggg gaatacggga
     1381 aaaaccggac gaaaacggcg atgatttttt caggctggca gcatggctca tcgcttcctt
     1441 tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg ccatcatcga
     1501 catggcatta ctggtgagtt tccatgccgc attactgtcc ttcggcaacg cttcgatgat
     1561 gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg atattcagaa ggtcagccac
     1621 acggcgggca ggctctgtca gcccgctgtt tctgagttcg gtacgggaaa tctgtatttc
     1681 caggttaagt ctgttaatca gacgattctg ttcgccgcgt tctgtttcga taccgcgtgt
     1741 ttccatcgca caggcagact ttccgaggtg aataccaggc gttttctcaa gcccctgtcg
     1801 ctcgtatgaa cgatggtcta tctcttcctg ataaccggcg ttcgccagtg ccctgttcgc
     1861 atggtcagcc catgatgcac gccacgtctc ggcatgtgtc cggtcgttcc agtccctgac
     1921 ctttccaccg aatccagcag gtccgacagc tctcgtggtc agcatgatgt gcgcatgggg
     1981 atttgtttta tccatgtgat ggaacgccac atcagcaatc atgccctgac tgacaaagtt
     2041 ttctctgaca aaggcgagaa cggtttcccg tgcggcatcc tgcggtagtt cgcgggggat
     2101 ggcgagttca atttcccgcg caagttgtga gttcttccgg cgttcggcct tctcaacggc
     2161 attccagagt tcggcacggt tcgtacacca ggcaggggct gtcgcgggag tcagaatcac
     2221 agcttcagcc acaccgcttt tgcgggtgta gtcgtgagtc agtccggtac gctcatcaag
     2281 catcagtgaa ccggaacgat aggcagcaga agcaacggcg ctgtagccgc tgtttctgga
     2341 gatgattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt tttgtggttt
     2401 tctttcagcc ccgcagggcg cacacaatcg aaacggagtg gagattgtat aagtgcgcac
     2461 ttcgtgcact tcgtgatttt ccgtggatta tgccagctca gtttttaaac taccagtgac
     2521 gcgctaaaat gcgatttttc atcgcgcacg gagttacatc atgagcagaa cactcgaaca
     2581 gaagattgct gatgctgagg ccagattaca gcgcctgaag gcgaagagca ggagtctgga
     2641 cacagcgcag aaggtcattg tgggtgcagc attgctggca aaggtcagaa agccggagga
     2701 ggtgcagttg cgtgcctggt tactccagtt cctgaaggca gaggtgacac gacaggctga
     2761 tgtgacgcgc atactgccgc tgattaacga gctggaagcg ctgccaggac agtgattgta
     2821 tcgtccgctc gccgcagtcc cagcgaccga gcgttagcga gcgagtgggc gaggaagcgg
     2881 aagtgaatcc gtcgcctgat tgagcacata cgcacggttt ttcgggaggt gatatgcgct
     2941 gaacccgcat gaatactgga tttccacagg gttggagggg gtgtattcgg aacttgcaat
     3001 tctgcttttt tgcaacgcat tgtttttatt ggttttttgt tttcagcact ccaagttatc
     3061 cacaggctcg tcgttaagtt tttagatctt gtttttaaga tcttgttttt aatacttgtt
     3121 ttagcgccaa aattccttat agaaaacaaa atgttacaac gctataggtg atatgattta
     3181 aggtaaaggt gatataattt aaggtgcttg ggtgaggtaa tttaaggtaa aggtgaggag
     3241 atttaaggta atattcacag gaaaatgggg ggtgaaaata taattgctca ccaatgctaa
     3301 aggtgatagg tttttatttc acctcatttt tggcctggtg gttaaaatga catcaaaccc
     3361 tctgatagcc tataagtcca atgcgctggt tgaggccagt tacaagctaa ctttacaaga
     3421 acagcgtttc ttattgctct gcataagccg acttaactcc ggtactgacg tagcttcacc
     3481 tgaattgcaa aagacaatga ccattacagc tgcggaatat tttgattcat tccctgatat
     3541 ggggcggaaa aatgccgagg ttcagctaca ggaagcgatc gatcgtttgt gggacaggtc
     3601 gatcattctc aaggacgacg agaagcgcga ggagttccgc tggattcagt atcgggcaca
     3661 gtacgcgagg ggagagggga aagcacaaat cactttttct gacgcggtaa tgccttatct
     3721 gacacagttg aaggggcaat tcaccagagt cgtcattaaa aatatttcta atttatcaag
     3781 aagttacagc ataagaatat acgaaattct ccagcaattc cgcagtactg gggaacgtat
     3841 tatcgccctc gatgatttta aatcatcact gatgcttgat ggtaaatata aagatttcaa
     3901 aacgttaaac agggatctaa taaaaccctg tgttgacgaa ctgaacaaaa aaagcgacct
     3961 ggctgtaacg gttgagacga tcaaaaaggg gcgcacggtt gtagccctgc attttcgctt
     4021 caaggaagac aaacagatca agatgacgat ctagaatcga ttttaaggca cctaaaacgc
     4081 tccctgctgc gttttt
//
