LOCUS       NZ_AP027836             8895 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.17.03CC plasmid p2017.17.03CC_7,
            complete sequence.
ACCESSION   NZ_AP027836
VERSION     NZ_AP027836.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562605
            Assembly: GCF_036505855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 115x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036505855.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:46:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,606
            CDSs (total)                      :: 5,482
            Genes (coding)                    :: 5,110
            CDSs (with protein)               :: 5,110
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 372
            CDSs (without protein)            :: 372
            Pseudo Genes (ambiguous residues) :: 0 of 372
            Pseudo Genes (frameshifted)       :: 109 of 372
            Pseudo Genes (incomplete)         :: 271 of 372
            Pseudo Genes (internal stop)      :: 48 of 372
            Pseudo Genes (multiple problems)  :: 54 of 372
            Pseudo Genes (short protein)      :: 4 of 372
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027836.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.17.03CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.17.03CC_7"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            join(<8888..8895,1..145)
                     /locus_tag="AABK32_RS27825"
                     /pseudo=""
     CDS             join(<8888..8895,1..145)
                     /locus_tag="AABK32_RS27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            complement(2275..2469)
                     /locus_tag="AABK32_RS27830"
                     /old_locus_tag="VEE61_54010"
     CDS             complement(2275..2469)
                     /locus_tag="AABK32_RS27830"
                     /old_locus_tag="VEE61_54010"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_116835902.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCRKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            3087..3281
                     /locus_tag="AABK32_RS27835"
                     /old_locus_tag="VEE61_54020"
     CDS             3087..3281
                     /locus_tag="AABK32_RS27835"
                     /old_locus_tag="VEE61_54020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041629.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELGNSIQTHFEAQYGTEP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3930..4073
                     /locus_tag="AABK32_RS27840"
     CDS             3930..4073
                     /locus_tag="AABK32_RS27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(4268..4399)
                     /locus_tag="AABK32_RS27845"
     CDS             complement(4268..4399)
                     /locus_tag="AABK32_RS27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            4429..4719
                     /gene="cki"
                     /locus_tag="AABK32_RS27850"
     CDS             4429..4719
                     /gene="cki"
                     /locus_tag="AABK32_RS27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(4716..6362)
                     /gene="cka"
                     /locus_tag="AABK32_RS27855"
                     /old_locus_tag="VEE61_54040"
     CDS             complement(4716..6362)
                     /gene="cka"
                     /locus_tag="AABK32_RS27855"
                     /old_locus_tag="VEE61_54040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            6603..6893
                     /locus_tag="AABK32_RS27860"
                     /old_locus_tag="VEE61_54050"
     CDS             6603..6893
                     /locus_tag="AABK32_RS27860"
                     /old_locus_tag="VEE61_54050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315653.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTSPSA"
                     /name="hypothetical protein"
     gene            complement(6885..7163)
                     /locus_tag="AABK32_RS27865"
                     /old_locus_tag="VEE61_54060"
     CDS             complement(6885..7163)
                     /locus_tag="AABK32_RS27865"
                     /old_locus_tag="VEE61_54060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315650.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQEAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            7285..>7560
                     /locus_tag="AABK32_RS27870"
                     /old_locus_tag="VEE61_54070"
                     /pseudo=""
     CDS             7285..>7560
                     /locus_tag="AABK32_RS27870"
                     /old_locus_tag="VEE61_54070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            7656..8884
                     /locus_tag="AABK32_RS27875"
                     /old_locus_tag="VEE61_54090"
     CDS             join(7656..7971,7971..8884)
                     /locus_tag="AABK32_RS27875"
                     /old_locus_tag="VEE61_54090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_094106523.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RNKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     Typing          1389..1467
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="87.0"
                     /evalue="1.57e-30"
     Typing          complement(3362..3443)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="93.0"
                     /evalue="6.91e-34"
     Typing          3460..4002
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.157"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccaggaattt ttaaactcac tccggggcac aactcaattt gcgggtactg attaccgcag
       61 caaagacctt accccgaaaa aatccagact aatggcagac accatttctg cggtttatct
      121 cgatggatac gagggcagac agtaagggat ttaccataat cccttaattg tacgcaccgt
      181 taaaacgcgc tcagcgcgtt tctggcgcaa aaaccgtaaa aagggatgtt ttccccgggg
      241 taaacaggaa aaatgcgtca ggaacgtttt cagcgcgttg catgactatg catgaaactg
      301 aatggcgatc ggtttgggcg cgtccgctgt ccctgaggct tattttcgta aattttcagt
      361 tcgtaattct ctgactcatt gctgcatatc agaccagaac gatcctagga gcgcccctag
      421 gatcgctctg taaaaaaatg cgacacgccc ctagggccgc ccctacatta attaactgtt
      481 tttttctaca gatagtcagt actgacccta agcagggtcg tcatagcgtc gccatagggt
      541 cgtcgacgac gtgcttttgt ataaatctga ataagaaaat atatttttag agccataacg
      601 acatgattta aaaagcaaat cagcaaaaag ttggttttgc gtagtgtgtg ggcatttcag
      661 gcatgagaac agactcgcgt ttttccggaa gaactgcggg gatttttgat taaacaatac
      721 tcaccgcaga gcggaatttt atgcaacgcc ggctgtgcgg cacagggatt tttaatcccc
      781 agtatgtcag agggtgcgga tgacggttcc cgtctggcgt tttcatgggc gccggttgtc
      841 aaaaaatgcc agcgtgatcg gtcgaaggat aaacgcagat taatcagtga tgattgagcg
      901 cgaatctgac aacgagtata gcgacacagc aacaaaaaag acgccagaaa cgcgcaggag
      961 tgttttagcg tgtctccggc gtgatgtcga gttgtgcggt aatgagtgag cgaaaaaaat
     1021 cccctggatt acagggggaa cccattatca gactttgaag tttatttgtt atttgatgga
     1081 gtgagcacga atcccagaga tgacgttcag gtggcaagga aacgcgctca gacgctttct
     1141 aacgcacttt tttgcatcgg gcaattattt tatatgtttt ggtgggttca ggctgtcaga
     1201 gacgttttga gcgcgtttct ggcgcttgct gctactgcca aaccatcccc atggccagtt
     1261 gatgccagaa acgcgcagga gttttgtagc gcgtctccgg tccctgatca atgaattccc
     1321 ggtcattaac atgcgtttac ccggaaacgc gccctgatgt tctatcgtat cgcgggaacg
     1381 gtgtcgaggg tgtcggggca aagccctgac caggtggtaa tcgcatagcc gtgcgtgcgc
     1441 ggttatacga ttacacatcc tgtcccggaa tcaggattgt ttttcgcgca caactgcggc
     1501 aaattcaccc cgttgcggtt gcaacgatgg gtatatcaaa actacgaaaa ttattcaggc
     1561 atttgctacc ccccttgccc tggcctccac cagtcacggg ggattttttt gttgtctctc
     1621 atgacgaact acggcaaatt tctgacgttg caaactgcaa cacataaaac acacacaaaa
     1681 ctcacaattg ccatgtcctc attgacaccc ccttgccata tagggggatt ttttcgcctg
     1741 tcctgattgt cttttgccga tggttacggc agattggaaa agagcgcatt tgtggtgctc
     1801 tctccagaat aatatttctc attactcaaa ctctgatggc ttccccctgt aatccagggg
     1861 gattttttaa tttatcgcta tgcttcgtaa ttcattcaga atacagacag tatggttaat
     1921 cagtttttca tcatttttgc agtgtttgcc ctgtcctgca tcagaacggt aaatacagga
     1981 atctctttat gacgaatcct ccatattcag gattttttat gtagcgtcta ctacatgcga
     2041 tccgttggta aaccgttagt tatatattaa ttatatctta attatataca caccaggtta
     2101 gttatacctt ggttatatgt taattatatc ttggttatac ttttgttatg cgtcagttac
     2161 agaggcgttt tgagggtaaa ttctggaggc acgtgaccga ctgtatacaa aatgctggcg
     2221 cattttgata cgtcgacgtg ccaaagggga ctcccctttg aaaccccgcc agaattaaaa
     2281 ccggcctgtc tttctacaat attcagctct ggctatacac caacctatat tttctccttt
     2341 ggcataatga agcgcatcat tccatttttt ccatgctcca atgtaatcgc cttcacgctc
     2401 cagtctgaca gcttcgtttg cgggtgaaaa atatttattt tctaaataaa ttgctttgtc
     2461 ctgattcata ctcgtattta tatttttatt ggaattaaaa agagcggcct gtcgggttaa
     2521 tacatattgg catgactgtt gatatatcag cccaactgca cgctcgtaat cgttttaacg
     2581 tgattttcgc aatatggcaa tccgggttcg tctgtccggt aatcaataaa aaccgtgtga
     2641 aaaacgggac aggatgtgca tttgtcggac cgcgcacaca cgaccggaca aacgccaccc
     2701 ggtcatggct gcgccccgac accccgcgaa cgtctctggc cagaactggc catcacgccg
     2761 ggtcacgttg tcagatgtaa aaaaatcacg cggtaaacgc cgttattcgc gggtttgcgc
     2821 aagcacctcc cgtgcgtcac tttgtcaaaa cgtcaaagtg acgcacatca ggaaccggcg
     2881 gaacgttcat gtttatgacg acaactgcac acaacgcaga cagaaaatcg cgcacatatt
     2941 cagcaaaaaa taagcgtcac ggggtgttaa tgtcgcgcag cccacacacg acggggcttg
     3001 tgtgataatt ttcagtgacg tcacctggcg tgatgaccag gtgacgcaca gccggacgta
     3061 aacaccagta acggagaagg gagaacgtga acaaacagca gcagaccgcg ctcaacatgg
     3121 cgcgatttat caaaagccag agcctgacgt tgctcgaaaa actggatgca ctggatgcag
     3181 acgaacaggc cgccatgtgc gaacgactgc acgaactggc ggaagaactc ggtaacagca
     3241 tacagacaca ctttgaagcg cagtacggca cggaaccata aaggggctgg cgaaagcggt
     3301 cgcggcttgt ccgtgagtgt tttcgccagc agacaagctc gtcagggcgc atcagcgggt
     3361 tttagcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     3421 ggcttaatca tatggcatca gtgcgggttg tatgaaaagt gcaccatgcc gggtgtgaaa
     3481 tgccgcacag atgcgtaagg agaaaatgct cgtccaggcg cttttccgct tcctcgctca
     3541 ctgactcgct acgctcggtc gttcggctgc ggcgagcggt atctgcccac tcaaaagcag
     3601 tgatagtgtt atccacagaa tcaggggatg taagcaagaa acgaaaaccc cgaaaaaggc
     3661 cacgccggag gcgctttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccccg
     3781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg
     3901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttaacgccac
     4021 tggcagcagc cattggtaac tggatagtgg atttagatac gcagaactct tgaagttgaa
     4081 gccttatagc ggctacactg gaaggacagc atttggtatc tgtgctccac taaagccagt
     4141 tacccggtta agcagtcccc aactgactta accttcgact aaaccgcctc cccaggcggt
     4201 tttttcgttt acaggcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4261 atcttttcta ctgaaccgcg atccccgtca gttcagaaga cgaagatggt gcaacggttc
     4321 ctccttgtac atcacgaata tagtttgctt gacatcctga caagaataat attgtaatta
     4381 gaaaaattat tattttcata tttctttcca acaaaagtaa aaatacttat gcatttaaaa
     4441 tactacctac ataatttacc tgaatcactt ataccatgga ttcttatttt aatatttaac
     4501 gacaatgata acactccttt gttatttata tttatatcat caatacatgt attgctatat
     4561 ccatactcta aattaaccat atctagatat atcaaagaaa atacaaagtt aaaaaaagaa
     4621 ccctggtact tatgcaagtt atctgcattg ttttatttat taatggcaat cccagtagga
     4681 ttgccaagtt tcatatatta cactctaaag agaaattaaa tccctaataa ctcattaagt
     4741 ttgctcaact catcatcccc tatgtaagaa gaaacaatac ctgtgacaat tgcaatcccc
     4801 cagaaaccaa gaggcacacc aacaataaaa ctaaacatta atgccacaat ttttgccaca
     4861 cctacatcga ccgcactttt ctccagcgtg acaaacaaag gtcgccagtt accagtttct
     4921 attgcatttt taaaatcaga accaacatca tatagaaaag atactcgact ggtgatttta
     4981 agcgactttg atatcttcgt taaattctta gataactcat cataattaac agactctaac
     5041 gcattaaaaa tagcatcccg atcaacctta ctgaatttct tatccagcac attcttatac
     5101 ttttcaaatg ctgccagagc ttcatcaaca ccttgaattt tctttccttt agacttttcc
     5161 gctaaatcct gagcaatttt tgcgtatttt tctccatatt gttcggttat atattgataa
     5221 aatccaacca tagtatcaac agcatctttt atctggctct tttcaagagc atcctgagct
     5281 tcttttaatt tctgctctgc ctctttaacc tcagcggcct taccatccct gaaactaaca
     5341 gcattcttaa gttcattttc aactgcggag tactcagcct gcttagcttc aagctgacgc
     5401 tgtagttttt tctgaacgtc acgccatccc ggaaatccgg tgaccttcac gtcaacactc
     5461 ttctgagcgt tttcaagctc tcctgcaact ctggaaacag tagcttgctt actctgtaca
     5521 tcactttcag ccttcgccag ttcagctttc gcctcagcta aacgcttctc tgcttccgcc
     5581 accgcctgtt tctcttcatt aagagtttca tgttcagcga cctctgcatt ttcacgagcc
     5641 ttttcttcag cttccttctg tttattcaca tcaccaattt tactttgcaa tgtattttta
     5701 taagaattaa gtttgctaat atctgcatca agctgatttg attttttttg caactcatca
     5761 acatccctct caagatcagt gataccatga taagaatgat gcttgaatac ttttttcatc
     5821 tcctcgattt tcttctgttt ttcactaatc tgtgtagcaa ttttattctt ctgcttttgt
     5881 ttttcattaa ttacattacc caccaccttc gaactcttat ccatatcact gacctgagca
     5941 ttcgttggtg cagtattaac tgaagcagtg ttattgttgt tatttcctga gctttctgca
     6001 aaaagtgatg gcatatcact aattaaagaa ttaagaactc tggagacccc tccaaatgga
     6061 ttatcaacca gagttgaatt ctcttctgtc ataacaatac cattcatcac cggaaggcca
     6121 tcattattaa tgacaacatc accccacgga gtcagatatg actcaccggt tttcatcaca
     6181 gttgatgtag aaccagatga atttgaattt ccctgaccac cattattacc atgcccagag
     6241 ccaccacccc agtgaacccc actattacta ttattatttc caccttgctg attcagattc
     6301 gcccctgttc cccccataga ctcaccagca gttggtccat atccacttag ttctttagcc
     6361 ataaattcct ctttgataat taaaacaata aattaaaaac aacgtactgt atatataacc
     6421 actggtttta tgtacagtgg aaacctacta ctcagcattg cccatgtcaa gaacatggat
     6481 tttcattttt gcaataaaga tcacactaca aggaggcaga cattgagaac gtcgaaacag
     6541 aacaccggag caaatcagga tgagatataa aactgttgga tcatgaaaaa acggagaacg
     6601 atgtgagcaa atctccccgc cataaactga acaaaacaga caagcgactt ctcgacaccc
     6661 tggttgctgc cggatatgag catgacaaag cccgtgacct catccagaaa caggtttaca
     6721 cgctgacact ggcagatcag cgtcatgtgg tcagtgaaat cagtaatggt gtgaatccca
     6781 cccaggctta ctcggcggta taccaggcaa gacgcattcg cctcgcccgt aaatatctga
     6841 acggtaaaaa ggttatggaa gaaaccgggg aaaatacgtc cccatcagcg taaggatttc
     6901 ttttgccgct ccagagactc cagtttttta cgcaaatcct ctcttttttg ggcatctctg
     6961 gtgccagcca gctctgctct caactcatcg atctgaagtt gtatcttcag tctgttactg
     7021 aacattttct gtctggcatt aacatccgca acaatgccgt tttttgtctt ctcggcctct
     7081 tgttgaaaaa cattgctgtc cgcatgactg gcaaccgaag cagaaagaac actaaaaagc
     7141 aggactggca cacatttttt cacgggatta ttcctgactc attgaccatc aaatcacatt
     7201 gggagtaaac cgacgtatga taagagatac tcttcggaga tataactccc tgagtatcaa
     7261 gattaaaaac gcaaggagat gtttatgaga tctgccgctg ccaggctgct tctgatacct
     7321 ctgataacag caacaatagc tcttacagga tgcacaccaa agaccagcct ggaacgacat
     7381 acccggcatt atgtttatgc ttcagatgat ggatttgatc ctaacttcta cacccagaaa
     7441 gcagacacca tacgtatgat ggtgcctttt tttcggcagt tctgggatat gggagctaaa
     7501 gataaagcga caggaaaatc acggagtgat gtgcaacaac gcattcagca gtttcacagc
     7561 caggtagact ggccccctga atctccagac aaccaatatc acttaaataa gtgatagtct
     7621 taatactagt ttttagacta gtcattggag aacagatgat tgatgtctta gggccggaga
     7681 aacgcagacg gcgtaccaca caggaaaaga tcgcaattgt tcagcagagc tttgaaccgg
     7741 ggatgacggt ctccctcgtt gcccggcaac atggtgtagc agccagccag ttatttctct
     7801 ggcgtaagca ataccaggaa ggaagtctta ctgctgtcgc cgccggagaa caggttgttc
     7861 ctgcctctga gcttgctgcc gccatgaagc agattaaaga actccagcgc ctgctcggca
     7921 agaaaacgat ggaaaatgaa ctcctcaaag aagccgttga atatggacgg acaaaaaagt
     7981 ggatagcgca cgcgccctta ttgcccgggg atggggagta agcttagtca gccgttgtct
     8041 ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga accgatgact ggatggatgg
     8101 ccgccgcagt cgtcacactg atgatacgga tgtgcttctc cgtatacacc atgttatcgg
     8161 agagctgccc acgtatggtt atcgtcgggt atgggcgctg cttcgcagac aggcagaact
     8221 tgatggtatg cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct
     8281 gttgcttgag cgaaaacctg ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc
     8341 cgtgaaagaa agcaatcagc gatggtgctc tgacgggttc gagttctgct gtgataacgg
     8401 agagagactg cgtgtcacgt tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc
     8461 ggtcactacc ggcggcttca acagtgaaac agtacaggac gtcatgctgg gagcggtgga
     8521 acgccgcttc ggcaacgatc ttccgtcgtc tccagtggag tggctgacgg ataatggttc
     8581 atgctaccgg gctaatgaaa cacgccagtt cgcccggatg ttgggacttg aaccgaagaa
     8641 cacggcggtg cggagtccgg agagtaacgg aatagcagag agcttcgtga aaacgataaa
     8701 gcgtgactac atcagtatca tgcccaaacc agacgggtta acggcagcaa agaaccttgc
     8761 agaggcgttc gagcattata acgaatggca tccgcatagt gcgctgggtt atcgctcgcc
     8821 acgggaatat ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtctggaaat
     8881 ataggggcaa atcca
//
