LOCUS       NZ_AP027824            10103 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.17.01CC plasmid p2017.17.01CC_2,
            complete sequence.
ACCESSION   NZ_AP027824
VERSION     NZ_AP027824.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562603
            Assembly: GCF_036505675.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 67x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036505675.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:10:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,673
            CDSs (total)                      :: 4,556
            Genes (coding)                    :: 4,337
            CDSs (with protein)               :: 4,337
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 59 of 219
            Pseudo Genes (incomplete)         :: 140 of 219
            Pseudo Genes (internal stop)      :: 49 of 219
            Pseudo Genes (multiple problems)  :: 30 of 219
            Pseudo Genes (short protein)      :: 4 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027824.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.17.01CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.17.01CC_2"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(join(9182..10103,1..47))
                     /locus_tag="AABJ82_RS23255"
                     /old_locus_tag="VEE60_45160"
     CDS             complement(join(9182..10103,1..47))
                     /locus_tag="AABJ82_RS23255"
                     /old_locus_tag="VEE60_45160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338383264.1"
                     /translation="MSADGSTVVGEAAYDLTAGGTGRHAFRWTSAGGMQDLGDLTANQQ
                     GYSSATAVSADGSTVVGEAAYDRTAGGTGRHAFRWTSAGGMQDLGDLTANQQGYSSATA
                     VSADGSTVVGGADYDRTAGGTGRHAFRWTSAGGMQDLGDLTADQQGASSAYAVSADGST
                     VVGGANYDRTAGGTDYHAFRWTSAGGMQDLGDLTANQQGYSHATAVSADGSTVVGWANY
                     DLTAGGTGRHAFRWTSAGGMQDLGDLTADQQGASSAYAVSADGSTVVGWANYDRTAGGT
                     DYHAFRWTSAGGMQDLGDLTADQQGYSSATAVSADGSTVVG"
                     /name="hypothetical protein"
     gene            575..2095
                     /locus_tag="AABJ82_RS23260"
                     /old_locus_tag="VEE60_45050"
     CDS             575..2095
                     /locus_tag="AABJ82_RS23260"
                     /old_locus_tag="VEE60_45050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_338383265.1"
                     /translation="MAIARLSMKVGKAGKAGPHAAYIAREGQYANRLERGERLEATEAG
                     NMPASAQSNPLAFWQAADAYERKNGTTYREMEIALPRELDAEQRAALVREFVRQEIGDR
                     HAYQWAIHVPTAADGGEQPHVHLMFSERQRDGIERDPEQYFKRYNAKAPEKGGARKGYG
                     PSAGQTLTKAERAAELKELRRRWEAMCNAHLERAGVEQRIDMRSHAERGTGLEPERKQL
                     PSQWRGQGRANVIEFRQARAELAEARQELARAEVIHLEAERQFEAMPAAELVEKWDTTT
                     KELAKGYRQRADRLEHRLRTEIHDIGDKRYRARVDHAEKRPAEVKDTLWNRITGKVAEY
                     GKALADWQGTMKRIEGWKQRREADLMRRLKRLEGYLSRTHYRDKATTKAERTLARKYPA
                     WAQRVPAAREQVAAERAQQRALQQAAQGMAKQIVSLAAAHQKGRVQGVSRELGRVLDAV
                     NAMPGRSSQKAATLAAELRERPDLMQTYQPLLAPHQRTLQRSRGGMER"
                     /name="MobA/MobL family protein"
     gene            complement(2188..2880)
                     /locus_tag="AABJ82_RS23265"
                     /old_locus_tag="VEE60_45060"
     CDS             complement(2188..2880)
                     /locus_tag="AABJ82_RS23265"
                     /old_locus_tag="VEE60_45060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344542.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_338383266.1"
                     /translation="MALAHKAQGYDVAEKRIVAAAVSKLGSRRVLKPGEVPRTKITAAE
                     YAETFEVDMNTAYDQLQGAAKQLYNRSITFYEPAHKRNGKPLPPTRVQMRWVGSVKYQD
                     GEGWVELAWWPDLLRHLTGLQRQFTTYQLQQASALRSGYSWKLLELLMRFESTGWAEYT
                     IEDFKASMDAPPSLSDFGQVKRRIIDPAVKELTEKDGWLIQWQPIKAGRKVKALRFTFM
                     RDPQGRLL"
                     /name="replication initiation protein"
     gene            3356..3508
                     /locus_tag="AABJ82_RS23270"
     CDS             3356..3508
                     /locus_tag="AABJ82_RS23270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866066.1"
                     /translation="MKKAGRQSRPTFRLLAAGLAFALRRVEDGPAKREHGFWIVQSKSR
                     GGRRV"
                     /name="hypothetical protein"
     gene            3637..3891
                     /locus_tag="AABJ82_RS23275"
                     /old_locus_tag="VEE60_45070"
     CDS             3637..3891
                     /locus_tag="AABJ82_RS23275"
                     /old_locus_tag="VEE60_45070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_257866067.1"
                     /translation="MRLVMTLAAVAVLTACNPASSDEAADARPVHDVAYFLDHPDERKA
                     QIAECRNNPGELADTPNCKNAMAAQQKAMFRGTGMPSIR"
                     /name="EexN family lipoprotein"
     gene            3903..4535
                     /locus_tag="AABJ82_RS23280"
                     /old_locus_tag="VEE60_45080"
     CDS             3903..4535
                     /locus_tag="AABJ82_RS23280"
                     /old_locus_tag="VEE60_45080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477436.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_257866068.1"
                     /translation="MAASKVARVYIRVSTDEQDVRRQESIIESAKAAGYYVAAVYREKA
                     SGARADRPELLRMIADLQAGEVVIAEKMDRISRLPLPEAEQLVQAIRDKGARLAVPGVV
                     DLSELTETAEGVARIVLQSVQDMLLRLALQMARDDYEDRRERQRQGIELAKAAGRYRGR
                     KPNRALHARVVALREGGSSIAETARLAGCSESQVKRIWASRRQIPAA"
                     /name="recombinase family protein"
     gene            4714..5364
                     /locus_tag="AABJ82_RS23285"
                     /old_locus_tag="VEE60_45090"
     CDS             4714..5364
                     /locus_tag="AABJ82_RS23285"
                     /old_locus_tag="VEE60_45090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400008.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_257866069.1"
                     /translation="MKILVLASQKGGAGKTTLAAHLAVAAEQAGAGPAVLLDTDPQGSL
                     SAWWNSREDDTPALAAASLAELPAKLRALAEAGFKLAVIDTPPAITEAIRDVVKLADLV
                     LIPTRPSPHDLRAVGSTVDIAQETGRPFAFAVTQAKPTARLTVQAVAALSAHGAVAPAI
                     VHDRVDYAASMVDGRTVGESDPKSRSAEEISQLLAFVQERMNAKKITKKKETV"
                     /name="ParA family protein"
     gene            5366..5623
                     /locus_tag="AABJ82_RS23290"
                     /old_locus_tag="VEE60_45100"
     CDS             5366..5623
                     /locus_tag="AABJ82_RS23290"
                     /old_locus_tag="VEE60_45100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005918078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866070.1"
                     /translation="MATKKPADLTGLVATKGSAAPVADMPTCTPKVKAEAEASANNVPL
                     NFRVSAELRRRFRMFAAAHDLKLNELLRLAFDEYEQRHNN"
                     /name="hypothetical protein"
     gene            5651..6646
                     /locus_tag="AABJ82_RS23295"
                     /old_locus_tag="VEE60_45110"
     CDS             5651..6646
                     /locus_tag="AABJ82_RS23295"
                     /old_locus_tag="VEE60_45110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="WP_257866071.1"
                     /translation="MSKSNGPQPIGSLLGNIVPREVKRAADATASKGQSNKSLSFISRQ
                     KINLIDAAAEIATNPPSGEDMAFTHAVLCQVGLPRAKVEGREFMRQSGAAWVNVQAGYL
                     DEGNGPVLQPIPYGVMPRLALAWVSTFAVRNKEREIKIGDSAAEFLSLMGLDDQGARYA
                     TLRKQMHALAACRLQLGFKGRTFNGQPVEQFDAWMANRDTKQRALWPGTMVLSEGYFTS
                     LMQSAVPLDNRALMALKGSALALDVYAWLAHRLHRIEGRGVTLHWKSLREQFAQEYTGK
                     DADKDFKKKFVPALKKVLAVYPQAKVKPVTGGVLLLGSPPPISYKGGPTE"
                     /name="replication protein RepA"
     gene            complement(7147..7386)
                     /locus_tag="AABJ82_RS23300"
                     /old_locus_tag="VEE60_45120"
     CDS             complement(7147..7386)
                     /locus_tag="AABJ82_RS23300"
                     /old_locus_tag="VEE60_45120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866072.1"
                     /translation="MTQDKWLAERLAYLRGLKAPSDQQRLLLMLADKPDRTADAVRIIN
                     AEKAAERKARPTICRPASPCPSKAVAASRCLRQP"
                     /name="hypothetical protein"
     gene            complement(<7386..7583)
                     /locus_tag="AABJ82_RS23305"
                     /old_locus_tag="VEE60_45130"
                     /pseudo=""
     CDS             complement(<7386..7583)
                     /locus_tag="AABJ82_RS23305"
                     /old_locus_tag="VEE60_45130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7567..7710)
                     /locus_tag="AABJ82_RS23310"
                     /old_locus_tag="VEE60_45140"
     CDS             complement(7567..7710)
                     /locus_tag="AABJ82_RS23310"
                     /old_locus_tag="VEE60_45140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_257866074.1"
                     /translation="MSVSLRLPPELNQRLSNLAERELEAVRAGKSKAVPLEDVMKQYGL
                     AD"
                     /name="hypothetical protein"
     gene            complement(7849..9120)
                     /locus_tag="AABJ82_RS23315"
                     /old_locus_tag="VEE60_45150"
     CDS             complement(7849..9120)
                     /locus_tag="AABJ82_RS23315"
                     /old_locus_tag="VEE60_45150"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="WP_338383263.1"
                     /translation="MQDLGDLTADQQGYSSATAVSADGSTVVGGADYDLTAGGTGRHAF
                     RWTSAGGMQDLGDFTSDQQGSSSATAVSADGSVVAGIASNDAGEMRAFIYKAPQSPTDP
                     NLPIQDYENVLKSFPKLAADKELAAAQQQLGMQRLLTSQCSAGQAEQSCLVVSGSLDTA
                     GSDGPIGRRQQTAGTVTLGRGLDERWTVGASLSLAHSSLHHNAVNPKNAYGVSGWVNYS
                     EHGLSGLGWQMDAAIGYAKNKSTITRGQGLEHVHLVQGTSNLDTVAARIALGFGIQQRH
                     GWTLTPAVSVTQQNSKFAAYSESPSAFTASYDKSELKTTVLGVDLSAKKSLSDKSQLRL
                     AVGVEQDLKTDRMKLAGVSDVPGAQRFNLESALERKKLRPYASVGYRYQVSAQGAVFVN
                     TGVMRDMFSNKPNVGVQVGYSARF"
                     /name="autotransporter domain-containing protein"
     Typing          5895..6042
                     /name="ColRNAI_rep_cluster_1998"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.248"
                     /coverage="98.0"
                     /evalue="2.97e-40"
ORIGIN
        1 agatcgtaag cagcctcccc cacgaccgtg ctgccgtccg cgctcacggc agtggcagag
       61 ctggctccct gttggtcggc cgtcaaggac cctaaatttt ctaaagactg cgctgaaacc
      121 gaaaagcagg ggagtgctgc caagcaaaag actcgaaact tcatgttgta tctcagtgaa
      181 ttggataacc gtggtttgtg agcctttaga aaatgcttta ccaccaaagc cccaaagccc
      241 caaagctcga caacgactat cttggtggat tcaaatctga actgcaacac ctgcaactgt
      301 taaggtcagc agatggtcat tcggagcttt gaggccacga atatatgcca gccgctcatt
      361 gagccatttg ttttgcgtca tcggtgctac tcactttggt cgtgatgcac cattctatgc
      421 aaatggtgac agcgcgtgtg catgcgctgt atgcttcctt ccatagaagg cgcgcttagt
      481 tgtttctcgg caagccgaga aacgcgcgtc aggggcaagc ccctgaaccc ctgcgcctgc
      541 ggcgcaacgg caacaccaaa ggaaaggcgg cggcatggcg atagcgcggc tctcgatgaa
      601 ggtaggcaag gccggtaagg ccgggcctca tgccgcctac atcgcccgcg aaggccagta
      661 cgccaaccgc ctagagcgcg gcgaaaggtt ggaagcgacc gaggcgggca acatgcccgc
      721 ctcggcgcag tccaacccgc ttgccttctg gcaggccgcc gacgcctacg agcgcaagaa
      781 cggcaccacc taccgtgaaa tggagattgc cctcccgcgt gagctggacg ccgagcagcg
      841 ggccgcgctg gtgcgcgagt ttgtacgcca ggaaataggc gaccggcacg cctaccagtg
      901 ggccatccat gtaccgaccg ccgccgatgg tggcgagcag ccgcacgtcc acctgatgtt
      961 ttccgagcgg cagcgcgacg gcatcgagcg cgacccggag cagtacttca agcggtacaa
     1021 cgccaaggcc ccggagaagg gcggcgcacg caagggctac ggcccgagcg cgggccagac
     1081 cttgaccaag gccgagcggg ccgccgagct gaaagagctg cgcaggcgct gggaggccat
     1141 gtgcaatgcg cacctggagc gggccggggt ggagcagcgc atcgacatgc gcagccacgc
     1201 cgagcgcggc accggcctgg agccggagcg caagcagttg ccgagccagt ggcgcggcca
     1261 gggccgggcc aacgtgatcg agttccggca ggcccgcgcc gagctggccg aggcccgcca
     1321 ggagctggcc cgcgccgagg tcatccacct ggaggccgag cggcagtttg aggcgatgcc
     1381 ggccgccgag ctggttgaga agtgggatac gaccaccaag gagcttgcca agggctacag
     1441 gcagcgggct gaccgccttg agcaccgctt gcggacggag atccacgaca taggcgacaa
     1501 gcggtatcgg gcgcgggtag accacgctga aaagcgcccc gccgaggtga aggacacgct
     1561 gtggaatcgg atcacgggca aagtagccga gtacggcaag gcgctggccg actggcaagg
     1621 cacgatgaag cgcatcgagg gctggaagca gcggcgagag gccgacctaa tgcgacgtct
     1681 caagcggctt gaggggtatt tgtcgcgcac tcactaccgc gacaaggcca caaccaaggc
     1741 cgagcgcacc ttggcgcgga agtatccagc atgggcgcaa cgggttccgg cagcgcgtga
     1801 acaggtggcg gcagaacggg cgcagcagcg tgcgctacaa caggccgcgc aaggcatggc
     1861 caagcaaatc gtgagccttg cggcagcaca ccaaaagggg cgtgtgcagg gcgtttcgcg
     1921 cgagcttggc cgagtcctgg acgcggtgaa cgctatgcct ggtcgcagca gccaaaaggc
     1981 ggccacgctg gccgcagagc tgcgcgaacg gccagacctg atgcagacct atcagccgtt
     2041 gcttgcgccg catcagcgca ccctacagcg gtcacgcggc ggcatggagc ggtagcgcgt
     2101 gtaagccttt ggcgatgccc gctgtaggct tttgcttact ctttggtgtc caactcggcg
     2161 gctttgcccg ctgtggcggg cggtgtgtta cagcagccgc ccctgcggat cgcgcatgaa
     2221 ggtgaagcgc agcgccttga ccttgcggcc cgccttgatc ggctgccact ggatcagcca
     2281 gccgtccttc tcggtcagtt ccttgaccgc aggatcgatg atccggcgct tcacttggcc
     2341 gaagtcactg aggctcggtg gcgcgtccat cgaggctttg aagtcctcga tggtgtattc
     2401 ggcccagccg gtcgactcga agcgcatcag cagttccagg agcttccagg agtagcccga
     2461 gcgcagggcg ctggcctgct ggagctggta ggtcgtgaac tgccgttgca ggccggtgag
     2521 gtggcgcagc agatccggcc accacgccag ctcgacccag ccctcgccgt cctggtactt
     2581 caccgacccc acccagcgca tttgcacccg cgtcggcggc agcggcttgc cgttgcgctt
     2641 gtgggctggc tcgtagaacg tgatcgagcg gttgtagagc tgcttggccg cgccctggag
     2701 ctggtcatag gccgtgttca tatcgacctc gaaggtctcg gcatattcgg cagcggtgat
     2761 cttggtgcgc ggcacctcgc cgggcttgag cacgcggcgg ctgcccagct tggacaccgc
     2821 agcggccacg atgcgtttct cggccacgtc gtagccctgc gccttgtgcg ccagggccat
     2881 ttgctcccgc ttcgtccctg ccatcctgac aacctccggg cctgcggccc tccgcgacgt
     2941 cgcccccctt ggggcacccc tgcggggttc ccccaccgcc tgcggcggcg gctccccctc
     3001 cggctaatcg gacggccgca agcggccgaa gcgttttgta gcgccggctg cgccggcgat
     3061 gggccggcct accggccggc aggctcggca gggaagagag ctggcggcca gtgtcgtgga
     3121 tctcctttga cctggttgcg ggctgatagg tggaaagcac caaaagaggc cggggggcac
     3181 gtgtaatctg atacgcctac ggctgcgctg gcagcccgtg gcggcgttct ttagggcagg
     3241 ggagggggaa ggtagcggcc cggccggtgc ggcgctccta gcgcgtccta tggctttcta
     3301 gcgtgaccga ttacacgttg aaggaatccc cggccggcga ccgggatccg attccatgaa
     3361 aaaagccggc cggcagagcc ggccgacctt tcgcctattg gccgctggcc tcgcgttcgc
     3421 gcttcgccgc gtcgaggatg gcccggcgaa gcgcgaacac ggcttttgga ttgtgcaatc
     3481 caaaagccgg ggcggccggc gcgtgtaatc gcaaacgctg gcgcagatgg atcggctttg
     3541 cgagcctagc gcaatagcat cgcggattca ccaaatgcgc cctagctgct atgctgggcg
     3601 catttaggaa tacccaactg cgccaaagga gaaactatgc gccttgtgat gacgctcgcc
     3661 gccgtggccg tgctgacggc ctgcaatcct gccagcagcg acgaagcggc cgacgcccgc
     3721 cccgtgcatg acgtggccta tttcctcgac catcccgacg agcgtaaggc gcagatcgcg
     3781 gaatgccgga acaaccccgg cgagctggcc gacacgccca attgcaagaa cgcgatggcg
     3841 gcccagcaga aagccatgtt ccggggcacc ggaatgccga gcatccggta ggaggcacca
     3901 aaatggcagc gtccaaggtc gcacgggtct atatccgcgt cagcaccgac gagcaggacg
     3961 tgcgccggca agaatccatc atcgagagcg cgaaggcggc cggctactac gtggccgccg
     4021 tctatcgtga gaaggcatcc ggcgcgcgcg ccgaccggcc cgagctgttg cgcatgatcg
     4081 cggacttgca ggccggcgag gttgtcattg ccgagaagat ggaccgcatt agccgcctgc
     4141 ccttgcccga ggccgagcag cttgtgcagg cgatccgcga caagggcgcg cggctcgccg
     4201 tgcccggcgt ggtggacctg tcagagttga ccgagacggc cgagggcgtg gcccgcatcg
     4261 tcctgcaatc ggtgcaagac atgctcttgc gcctcgccct gcagatggcg cgtgacgact
     4321 acgaggaccg ccgcgagcgg cagcggcagg gcatagagct tgccaaggcc gccggccgct
     4381 acagaggtag gaaaccaaac cgggcgctac acgctcgcgt cgtggccttg cgtgagggcg
     4441 gatcgagcat cgcggaaacc gcccggctgg ccggatgcag cgagagccag gtcaaacgaa
     4501 tatgggcctc ccgccggcag attccggcgg catagggcat cagaaaccgg cctccccttc
     4561 cctacccgtt tgcgattaca cgcgccggcc gccccggctt ttggattgca caatccagaa
     4621 agcacgcaag cgtgcttttg ttcaatagac tctgcaagat gcgtgctttt tggctatcat
     4681 taaagcgtcc caacacgaat caagagggcg catgtgaaaa tccttgttct agcaagccag
     4741 aaggggggag ccgggaaaac caccctagcg gcgcaccttg cggttgccgc cgaacaggca
     4801 ggagccggcc cggcggtcct gctcgacact gacccgcaag ggtcactttc ggcgtggtgg
     4861 aactcccgcg aggacgacac cccggcactg gccgccgcct cgctggccga gctgcccgcc
     4921 aagctgcggg cgctggccga ggctggtttc aagctggccg tgatcgacac cccgccggcc
     4981 atcaccgagg ctatccgcga cgtggtgaag ctggccgacc ttgtgttgat cccgacccgg
     5041 cccagcccgc acgacctgcg ggccgtaggc agcaccgtgg acattgcgca ggaaaccggc
     5101 cggcctttcg ccttcgccgt gacgcaggcc aagccgaccg cgcggcttac cgttcaggcc
     5161 gtggccgcct tgtccgcgca tggggctgta gccccagcca tcgtgcatga ccgcgtggac
     5221 tatgccgcct cgatggtgga cggccgcacc gtaggcgaga gcgaccccaa gagccgcagc
     5281 gccgaggaaa tctcccagct tttagctttc gtgcaagaac gcatgaacgc taagaagata
     5341 accaaaaaga aggaaaccgt ttaatatggc taccaagaag cccgctgatt tgaccggcct
     5401 tgttgccacg aagggcagcg ccgcgccggt tgccgacatg ccgacctgca cccccaaggt
     5461 gaaggccgag gccgaggcct cggcgaacaa tgtgccgctc aatttccgcg tgtcggccga
     5521 gctgcgtcga cgtttccgca tgttcgcagc ggcccacgac ctcaagctaa acgagctgtt
     5581 gcggctggcc ttcgatgaat acgagcagcg ccacaataac tagcccggcc atgccggtgg
     5641 agatcgcctt atgagcaaat ctaacggccc ccagcccatc ggttcgctgc ttggcaacat
     5701 tgtgccgcgc gaggttaagc gggcggccga cgctacggca tctaaagggc agagcaataa
     5761 gagcctgtca ttcatcagtc gccaaaagat aaacctgatc gacgcggccg ccgaaatcgc
     5821 cacgaacccg ccgagcggcg aggacatggc gtttactcac gccgtgcttt gtcaggtcgg
     5881 cttgccccgc gccaaggtcg aggggcgcga gttcatgcgc cagtccgggg ccgcgtgggt
     5941 gaacgtgcag gccggttatc tggacgaagg caacgggccg gtgctgcaac cgatccccta
     6001 tggcgtcatg ccccggctgg cgctggcgtg ggtgtccacc tttgccgtgc gcaacaagga
     6061 gcgcgaaatc aagatcggtg atagcgcggc cgagttcctt agcctgatgg gcttggacga
     6121 ccaaggggcg cgctatgcga ccctgcgcaa gcagatgcac gcgctggccg cctgccgtct
     6181 gcaacttggc ttcaagggcc gcaccttcaa tggacagccg gtcgagcagt tcgacgcatg
     6241 gatggcgaac cgcgacacca agcagcgggc gctatggccg ggcacgatgg tgttgtccga
     6301 gggctatttc acgtcactga tgcaaagcgc cgtaccgctc gacaaccgcg ccctgatggc
     6361 cctgaaaggt tcggcgctgg cgctcgatgt ctacgcttgg ctggcccacc ggctgcatcg
     6421 catcgaggga cgcggcgtca ctttgcactg gaagtctctg cgcgagcagt tcgcgcagga
     6481 gtacaccggc aaggatgctg acaaggactt caagaagaag tttgtgccgg ccctcaagaa
     6541 ggttctggcc gtctatccgc aggccaaggt gaagccggtc acgggcggcg tcctgctgct
     6601 cggttccccg ccgccgatct cttacaaggg cggtccgacc gagtaacgct aaccccttga
     6661 agtgtccgac aaacgtgtcg ttagatcggc cccccctaga ccccggcgat tcgtgtgcaa
     6721 cgcattgaat ctccggggtt ttatgtctct ggaagtgtcc gaaaacccca gcgggaccaa
     6781 aacggtgcac aaccctgtcg atagcgccac ttatccacgt taaatcggac cccccccacc
     6841 tcgataaatc ggaccccccc aagggtactt atccacgtta aatcggaccc cctttttccc
     6901 gttaaatcgg acccccccta aaccgatagg ttaagccttt aaaaaaagaa tcctataaat
     6961 ctaaacctgt aattggccgc cacaacctgt ggaaaacgct gctgcgacaa ggacatgggg
     7021 cacccctgcg gggttccccc accgcctgcg gcggcggctc cccctccggc ttcggcaatc
     7081 aggaagggaa ggggtcgcca aggcccgtag aagggcctac agcgcgtttt gagcgtaggg
     7141 gaagggtcaa ggctgccgaa ggcaccgcga agcggctacg gccttacttg ggcagggtga
     7201 tgcgggtcgg cagatcgtcg gacgggcctt cctctcggcg gccttctcgg cgttgatgat
     7261 gcggacggcg tcggcggtgc ggtcgggctt gtcggccagc atcagcagca ggcgctgttg
     7321 atcgctggga gccttcaagc cgcgcaggta tgccagccgt tcggccagcc acttatcttg
     7381 tgtcatatga ttcggtagtc ccccacgcag tacttccaaa gctcccccag cttggagcct
     7441 ttgagtgcct cgccaatgct gcgcgggtca tcaagttgcg ccacgcgacc gtgcaaaaag
     7501 ctcaggatgc gtcgggcgtg ttggtggtcg agctgcttga ggtttttgac agcaccttcg
     7561 gacagttcaa tctgccaagc catactgctt cataacgtct tccaaaggca cggccttgga
     7621 tttaccggca cgcaccgctt ccagctcacg ctcggccaag ttgctcagac gctggttcag
     7681 ttctgggggt aggcgaagag atacagacat ggcatcaact ccttttgaga tacatgtagc
     7741 tcgcatggta gcaccagacg caaaaaaggc gctttgagcg ccttttttgt tgccatcgat
     7801 aagcaggatt taccgtgagc tgaggaaatg tttgaacgct atgtcgcgtt aaaaccgtgc
     7861 gctataaccg acctgcacac ccacgttggg tttgttcgag aacatatccc gcatcacgcc
     7921 tgtgttcaca aacacagccc cctgtgcact gacttgatag cggtatccca cagaggcata
     7981 aggacgcagt ttcttgcgct ctaaagcact ctctaagtta aatcgctgcg ctcctggaac
     8041 atcagagacg ccagccagct tcatacgatc cgttttaagg tcttgctcaa cgcctaccgc
     8101 taaacgcagc tgcgacttgt cgctcaaaga cttcttggca ctcagatcca ctcccaacac
     8161 agtcgtttta agctccgatt tatcgtacga cgcagtaaag gctgaaggcg actcactgta
     8221 agctgcaaac ttgctgtttt gctgtgtcac agacactgct ggcgtcaaag tccatccatg
     8281 acgctgctgg atgccaaacc ccaaggcaat acgagctgcc actgtgtcta aattgcttgt
     8341 gccctgcacc aggtgaacat gctcaaggcc ctgaccacgg gtgatggtgc tcttgttctt
     8401 cgcataacca atggctgcat ccatctgcca gccaagaccg gacaagccat gctcgctgta
     8461 gttcacccag ccacttacgc cataagcatt cttaggattt actgcgttgt ggtgcaagct
     8521 cgagtgcgcc agcgacaagc ttgcccccac cgtccagcgc tcatcaagac ctcgccctag
     8581 tgtgacagtc cccgctgttt gctgacggcg accaattggg ccgtcagagc ctgccgtatc
     8641 caacgagcca gagaccacca agcagctttg ctccgcttgc cctgctgaac attgcgaagt
     8701 caacaagcgc tgcataccta gctgctgctg cgcagcagcc aactctttgt cggcggccaa
     8761 cttaggaaac gacttgagga cattctcgta gtcttggatc ggtagattag ggtctgttgg
     8821 cgactgaggc gctttgtaaa taaatgcacg catttctcct gcgtcattgc ttgcgattcc
     8881 cgccaccacg ctgccatccg cgctcacggc agtggcagag ctggatccct gttggtcgga
     8941 cgtaaagtcg cccaaatcct gcatgccgcc cgcactggtc cagcgaaatg catgcctgcc
     9001 cgtcccgcct gccgtgagat cgtaatcagc cccccccacg accgtgctgc cgtccgcgct
     9061 cacggcagtg gcagagctgt atccctgttg gtcggccgtc aagtcgccca agtcctgcat
     9121 gccgcccgca ctggtccagc gaaatgcatg atagtccgtc ccgcctgccg tgcgatcgta
     9181 attagcccac gaccgtgctg ccgtccgcgc tcacggcagt ggcagagctg tatccctgtt
     9241 ggtcggccgt caagtcgccc aagtcctgca tgccgcccgc actggtccag cgaaatgcat
     9301 gatagtccgt cccgcctgcc gtgcgatcgt aattagccca ccccacgacc gtgctgccgt
     9361 ccgcgctcac ggcataggca gagctggctc cctgttggtc ggccgtcaag tcgcccaagt
     9421 cctgcatgcc gcccgcactg gtccagcgaa atgcatgcct gcccgtcccg cctgccgtga
     9481 gatcgtaatt agcccacccc acgaccgtgc tgccgtccgc gctcacggca gtggcatggc
     9541 tgtatccctg ttggttggcc gtcaagtcgc ccaagtcctg catgccgccc gcactggtcc
     9601 agcgaaatgc atgatagtcc gtcccgcctg ccgtgcgatc gtaattagcc ccccccacga
     9661 ccgtgctgcc gtccgcgctc acggcatagg cagagctggc tccctgttgg tcggccgtca
     9721 agtcgcccaa gtcctgcatg ccgcccgcac tggtccagcg aaatgcatgc ctgcccgtcc
     9781 cgcctgccgt gcgatcgtaa tcagcccccc ccacgaccgt gctgccgtcc gcgctcacgg
     9841 cagtggcaga gctgtatccc tgttggttgg ccgtcaagtc gcccaagtcc tgcatgccgc
     9901 ccgcactggt ccagcgaaat gcatgcctgc ccgtcccgcc tgccgtgcga tcgtaagcag
     9961 cctcccccac gaccgtgctg ccgtccgcgc tcacggcagt ggcagagctg tatccctgtt
    10021 ggttggccgt caagtcgccc aagtcctgca tgccgcccgc actggtccag cgaaatgcat
    10081 gcctgcccgt cccgcctgcc gtg
//
