LOCUS       NZ_AP027820             6180 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.15.02CC plasmid p2017.15.02CC_2,
            complete sequence.
ACCESSION   NZ_AP027820
VERSION     NZ_AP027820.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562602
            Assembly: GCF_036505475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 152x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036505475.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:59:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,886
            CDSs (total)                      :: 4,769
            Genes (coding)                    :: 4,542
            CDSs (with protein)               :: 4,542
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 75 of 227
            Pseudo Genes (incomplete)         :: 154 of 227
            Pseudo Genes (internal stop)      :: 43 of 227
            Pseudo Genes (multiple problems)  :: 42 of 227
            Pseudo Genes (short protein)      :: 3 of 227
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027820.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.15.02CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.15.02CC_2"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(278..601)
                     /locus_tag="AABJ72_RS24360"
                     /old_locus_tag="VEE59_47090"
     CDS             complement(278..601)
                     /locus_tag="AABJ72_RS24360"
                     /old_locus_tag="VEE59_47090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001483787.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQRVQ
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            668..859
                     /locus_tag="AABJ72_RS24365"
                     /old_locus_tag="VEE59_47100"
     CDS             668..859
                     /locus_tag="AABJ72_RS24365"
                     /old_locus_tag="VEE59_47100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1479..1640
                     /locus_tag="AABJ72_RS24370"
     CDS             1479..1640
                     /locus_tag="AABJ72_RS24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_096111359.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHAKAPLAAAAGKQNVREKYR
                     EFLKWWPE"
                     /name="replication initiation protein"
     gene            2074..2997
                     /gene="yhdJ"
                     /locus_tag="AABJ72_RS24375"
                     /old_locus_tag="VEE59_47110"
     CDS             2074..2997
                     /gene="yhdJ"
                     /locus_tag="AABJ72_RS24375"
                     /old_locus_tag="VEE59_47110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399826.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008170 - N-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="WP_096111358.1"
                     /translation="MKIEFEARYFTDEQNKIIHGDALTELKKLPSESVDLVFADPPYNI
                     GKDFDGLVESWDDEEFLHWLFECIDECHRVLKKNGTMYIMNSTENMPYIDIKCRNIFHI
                     KSRIVWSYDSSGVQAKNYFGSMYEPILMMVKDPKKYTFNSKDILVEAKTGSKRALIDYR
                     KNPPQPYNTQKVPGNVWEFPRVRFLMEEYENHPTQKPTSLLKRIVLASSNPGDVVLDPF
                     SGSFTTGDVSKSLDRRFIGIEINEEYVKMGIRRLNIPSSYTPKELEKLKKRKTKNKSKN
                     VRGDDNDISDLLIDDLPSSQEDHTLI"
                     /name="yhdJ"
     gene            complement(2969..4000)
                     /locus_tag="AABJ72_RS24380"
                     /old_locus_tag="VEE59_47120"
     CDS             complement(2969..4000)
                     /locus_tag="AABJ72_RS24380"
                     /old_locus_tag="VEE59_47120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_096111357.1"
                     /translation="MVDFVKKIDELASKYNVSQRSVVYKYIEKKTRASGRDSKARRSLG
                     NLYALYVLCEAYNDGAKGGTSFSVLMQRMRALPFGSKLQNHPLDNRLNDEVRRSFLVQD
                     NMLPVQSVIEGTVKKRIISIDFLSEFGMDPGSSSLFITKAIDLYIDEITEKQNEFLFNI
                     ENSNGKEGILKFIENSFSPNADARLFEVVSFSVLKLYFSKKNIVINGREEFFQLFKTGR
                     TNANDGGIDFVLKPVGRFFQVTEVLDFKKYFLDFDKTNKFPMSFVVKTDLDPEGVMRLI
                     KVNAEKSMGKELANRYFGLFEEVFTLPTLRNIVGVISDSLEDLEELKRVIIENFKLEYG
                     LLD"
                     /name="type II restriction endonuclease"
     gene            complement(4000..4209)
                     /locus_tag="AABJ72_RS24385"
                     /old_locus_tag="VEE59_47130"
     CDS             complement(4000..4209)
                     /locus_tag="AABJ72_RS24385"
                     /old_locus_tag="VEE59_47130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321970.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_096111356.1"
                     /translation="MNPEDIKKVFGENLRRIRMEKNISQENLAFLSGLDRTYVSGIERG
                     KRNVSLVNINKIALALNVEIKELF"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4331..4585)
                     /locus_tag="AABJ72_RS24390"
     CDS             complement(4331..4585)
                     /locus_tag="AABJ72_RS24390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338386300.1"
                     /translation="MWLKFIGCHRGSFSGGLLPFLPVCCRDRTERVSAVRATDVMVNAN
                     GSGDVKKPPERWLFHFLDRVERHLRHQLWLVAPEGGGRS"
                     /name="hypothetical protein"
     gene            complement(5010..5243)
                     /locus_tag="AABJ72_RS24395"
                     /old_locus_tag="VEE59_47150"
     CDS             complement(5010..5243)
                     /locus_tag="AABJ72_RS24395"
                     /old_locus_tag="VEE59_47150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_141035767.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERIMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5250..5780)
                     /locus_tag="AABJ72_RS24400"
                     /old_locus_tag="VEE59_47160"
     CDS             complement(5250..5780)
                     /locus_tag="AABJ72_RS24400"
                     /old_locus_tag="VEE59_47160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_338386301.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGLRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     Typing          1079..1588
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.129"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(282..634)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.373"
                     /coverage="100.0"
                     /evalue="1.16e-63"
     Typing          complement(4907..6180)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.588"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctccacccac aggatgctgt actgattctt ttcgagaccg ggcatcagta cacgctcaaa
       61 gctcgccatc accttttcac gtcctcccgg cggcagctcc ttctccgcga acgacagaac
      121 acctgacgtg tatttcttcg caaatggcgt ggcatcgatg agttcccgga cttcttccgg
      181 attaccctga agcaccgttg ccccttcccg gtttcgctcc cttcccagca ggtaatcaac
      241 cggaccactg ccaccgcctt ttcccctggc atgaaactta actatcatcc tgcactctct
      301 gttccctgac cacctgccgt aagctgcgca actctctctc gatggccatc agtgcggcca
      361 ccacctgaac ccggtcaccg gaagaccact gcccgctgtt caccttcctc gcagtctgat
      421 tcaggttatt cccgatggcg gccagctgac gcagtaacgg cggtgccagt gtcggcagcc
      481 tcccggagcg ggcaaccggc tcacccagac agacccgccg catccatacc gccagctgtt
      541 taccctcaca gcgttccagt aatcgggcat gttcatcatc agtaacccgt atcgtgagca
      601 tcctctcgcg tttcatcggt atcattaccc ccatgaacag aaatcccccc cttacacgga
      661 ggcatcagtg actaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
      721 gacattaacg ctgctggaaa agctcaacga actggacgcg gatgaacagg cagacatctg
      781 tgaatcgctt cacgaccacg ccgatgagct ttaccgcagc tgcctcgcac gcttcgggga
      841 tgacggtgaa aacctctgac acatacagct cccggagacg gtcacagctt gtctgtgagc
      901 ggatgccggg agcagacaag cccgtcaggg cgcgtcaggc cgttgacctt gcgggtgtcg
      961 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct caatatgtta
     1021 cggcggagta acacttaatg aaagtgcttc atgtggcagg ggaaaaatgc agctgtaggg
     1081 cgtacaggag aaaatggagg ttacagtgat atgacgcctc ctcgctcact cggtcgcttc
     1141 cgctcctgac gttcggctgc ggcgggcgtt acctgcaaac ggataagagc aaaggcatac
     1201 ggcatggcaa gcaggacagg aaaaggatgc ggcgctgccg tttttccata ggctccgccc
     1261 ccctgacaag catcacaaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1321 ataaagatac caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt
     1381 tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca cgcctgacac
     1441 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1501 ccgaccgctg cgccttgtcc ggtaactatc gacttgagtc caacccggaa agacatgcaa
     1561 aagcgccact ggcagcagcc gctggtaaac agaatgtgcg agagaagtat cgagagttct
     1621 tgaagtggtg gcctgagtga ggctacactg gaaggacaga ttgggtgaca tgtctcgcac
     1681 aagactgtta ccacggttca gaagttcccc aacttactga accttcgaaa aaccacctcc
     1741 ccaggtggtt ttttcgttta cagagcaaga gattacgctc agatatagat gatctcaaga
     1801 ggatcttttg agatcctact gaaaactgac gaagcaatta agcatcaata ttgttagtgg
     1861 cagtctttga taaccctcag agcctcttca ccaccaccta agatttaagc attaacacat
     1921 gaaaatcaat aagttaaact ttaatacatt atatatgcaa cttatatatg tttttaaagg
     1981 caatcataaa gcataaaagt agagctatga aggttgtaca gcatcaattt tgacggtagc
     2041 atagtagact atgagtcaca ctaagggtaa aagatgaaaa ttgaattcga agccagatat
     2101 ttcacagatg aacaaaataa aattatccat ggtgatgctt taactgaatt aaagaagtta
     2161 ccatcagaaa gcgtagattt ggtttttgca gatccgccat ataatatcgg taaagatttc
     2221 gatggtcttg ttgagtcttg ggatgatgaa gaatttcttc attggctatt cgagtgcatt
     2281 gatgaatgtc atcgagtttt aaaaaaaaat ggcaccatgt atataatgaa tagtactgag
     2341 aacatgccat atattgacat aaaatgcagg aatatctttc atataaagag tagaatagtt
     2401 tggtcttatg atagctctgg ggttcaagct aaaaactact ttggttctat gtatgaacca
     2461 atattaatga tggttaaaga ccccaaaaaa tatactttta acagtaaaga tatcttggtt
     2521 gaagctaaaa caggttccaa aagagcttta attgattaca ggaagaatcc acctcaacct
     2581 tataatactc aaaaagtacc tggaaatgtt tgggaatttc cccgagtaag attccttatg
     2641 gaggaatatg aaaaccaccc aacgcaaaaa ccaacttctc ttctaaagag aattgtttta
     2701 gcttcatcaa atccagggga tgtagtttta gatccttttt ctggtagctt tacaacggga
     2761 gatgtatcta agtctctaga tagaagattt ataggtatag aaataaacga agagtatgta
     2821 aaaatgggca taagaaggtt aaacatacct tcaagttaca ccccaaaaga attagaaaaa
     2881 ttaaaaaaaa ggaagacaaa aaacaaatcg aagaatgtca ggggggatga taatgacatt
     2941 tctgatttat taattgatga tctgccctct agtcaagaag accatactct aatttaaagt
     3001 tttcaataat gactctcttg agttcttcaa gatcctcaag agagtcagat atcactccta
     3061 caatatttct aagggttggt aaagtaaaaa cttcttcaaa caagccaaaa tatctattcg
     3121 ctaattcttt gcccatactt ttttctgcat taacctttat taacctcata actccttctg
     3181 gatcaagatc cgttttaaca acaaaactca taggaaattt attcgtctta tcgaaatcta
     3241 aaaagtattt tttgaagtct aaaacctcag ttacttgaaa aaaccttcct actggcttta
     3301 aaacaaaatc tatcccacca tcattagcat tagtccttcc tgttttaaac agctggaaga
     3361 actcctccct tccatttatt acaatatttt ttttagagaa gtataatttt agcactgaaa
     3421 aggatacgac ctcaaacaac ctagcatctg catttgggct aaaggaattt tcaataaatt
     3481 tcaatatccc ttcttttcca ttcgagtttt caatattaaa aaggaattca ttttgttttt
     3541 cagttatttc gtcaatatat aaatcaattg ctttagtgat aaacaatgaa gatgaaccgg
     3601 gatccatgcc aaactctgaa agaaaatcaa tggaaattat tctttttttt actgttcctt
     3661 ctatcacact ttgcacaggc aacatattat cctgcactaa aaaacttctt cttacctcat
     3721 cattcaatct attatccaaa ggatgatttt gcagtttaga accaaaagga agcgctctca
     3781 ttctctgcat taaaacagag aaggaggtcc cgcccttcgc cccatcatta tatgcttcac
     3841 aaagaacata tagcgcatat aaattgccaa gtgaacgcct agctttacta tctctgcctg
     3901 atgctctagt ctttttttcg atatatttgt aaactacaga cctttgactt acattatatt
     3961 tagatgctaa ctcatctatt ttcttaacga aatccaccat taaaagagct cctttatttc
     4021 cacattcaaa gccaatgcta ttttatttat gttaaccaga cttacattcc gcttccctcg
     4081 ctctatccca cttacgtaag tcctgtccag tccggataaa aacgcaagat tctcttggct
     4141 aatatttttc tccattctga ttcgtcttaa gttttcacca aatacttttt tgatatcttc
     4201 tggattcata cattaaatcc gtaaaaaaat ttataattgc atttggtgac tcaaaaccca
     4261 acggacttta agtcacattc cgtgtttatt attgtcaaat ggttagtttt ctctatcggc
     4321 actattcggc ttaagaacgc ccccctccct caggagcaac taaccaaagt tggtgtcgta
     4381 aatgccgttc gactctatct aaaaaatgaa aaagccacct ttcaggcggc tttttcacat
     4441 caccagaacc atttgcattt accataacat ccgttgcccg caccgcagaa acgcgttcgg
     4501 tgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc acccgaaaaa ctgccgcgat
     4561 ggcacccgat aaattttaac cacatgcata gctatgcagc catgtgaatc acgcaggaat
     4621 tgccagccag agactgctga aacggatttt tttcatactt tcggaaaggc agaagacgga
     4681 accggagcgg aaaaacagaa gttcagggga atgagcgcga tctggcaata gaggcaaaac
     4741 acagcaacaa aagatacacc agaatcgcgc ccgtatgcgt tttaacgcgt tcctgtgttt
     4801 tcagcaaggt ctgactgaag cgcgttcccg gtgcccccga accaaccatt atcccaaaaa
     4861 atgcaccgga aacgcgctga caacatttta gcctgcctct gattaccatc ccagcgaagg
     4921 gccatccagt gtccgttcct gtatttccgg ctggcgctca cgctccaggg ataacgcatg
     4981 ttcgcgttcc tgtatcagcc gttcatccct tatctcctgt tctcgctgta taactggctc
     5041 aagcgttctg tctgctcgct caagtgctgc acctgctgac tcaactgcat gatccgctcg
     5101 ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacattt tctgcaattc cacgaaggcg
     5161 ctctcccatt cgttcagccg ctgcatatag tcctgttgca gttgctctaa tgcgttcagc
     5221 aaatgtcttt ccagctccgt catgctctgt tactcctgta ccagccttac ggcattctgt
     5281 ttcccgtttt ccatcgtcca gttcgtttcg gctttcatcc cctcgggcaa caccagaaaa
     5341 cgtccgttgc tcccctcgtg ataacgcaag ccccatgtct tcgcgttcag cttctccaga
     5401 ttctcgttct gcttcctgat ttcttccagg ttcgcctgta tcatcccgcc aagataccag
     5461 agcgtcccgc cactcacggt aaacaggaaa atgaccatcc ccagtaacat catgcccgta
     5521 ttccctgcca gctttaacac gtccttcctg tgctgcatca tcgcctcttt caccccttcc
     5581 cggtgttttt tcagtgattc ctctgtcgaa gctgtgaaca gggctatagc gtctttgatt
     5641 ttcgtctcgt ttgatgtcac agccttgctt acagattttt cgagcttgct gaactcgtcg
     5701 ttcagcattg tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc
     5761 gtctgtaaaa gattgctcat agaatgctcc tttcagcctg atattcttcc cgccgttcgg
     5821 gtctgcaatg ctgatactgc ttcgcgtcac cctgaccact tcaagcccgg cctctgtgag
     5881 cgcctgaatc acatcctgac ggccttttat ctccccgaca tgataaagag cgtctatccc
     5941 gcgtgtgacg ctctctgcaa gcgcctgttt cgtttccggc aggttatcag ggagagtcag
     6001 tatccggcgg ttctccgggg cgttggggtc atgcagcccg taatggtgat taaccagcgt
     6061 ctgccaggca tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc
     6121 ggtctgtagc tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg
//
