LOCUS       NZ_AP027806             2043 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.10.01CC plasmid p2017.10.01CC_6,
            complete sequence.
ACCESSION   NZ_AP027806
VERSION     NZ_AP027806.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562596
            Assembly: GCF_036504915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 39x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036504915.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:02:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,739
            CDSs (total)                      :: 4,619
            Genes (coding)                    :: 4,396
            CDSs (with protein)               :: 4,396
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 50 of 223
            Pseudo Genes (incomplete)         :: 166 of 223
            Pseudo Genes (internal stop)      :: 30 of 223
            Pseudo Genes (multiple problems)  :: 23 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027806.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.10.01CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.10.01CC_6"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            join(1477..2043,1..402)
                     /gene="mobV"
                     /locus_tag="AABJ86_RS23690"
     CDS             join(1477..2043,1..402)
                     /gene="mobV"
                     /locus_tag="AABJ86_RS23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(464..898)
                     /locus_tag="AABJ86_RS23695"
                     /old_locus_tag="VEE56_45440"
     CDS             complement(464..898)
                     /locus_tag="AABJ86_RS23695"
                     /old_locus_tag="VEE56_45440"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          2..399
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.241"
                     /coverage="96.0"
                     /evalue="1.6e-58"
ORIGIN
        1 cgtggcaccc gtagccaggt atcgatctcg ccggaggcgc tggagccgcg tgtattgcgg
       61 aagggcattt tcaccaaaga cgtggaagat caggcagcca tagccaaacg tctaagccac
      121 gcagtgaacg atggctttgc aggaaccatc gctatggcct ctcagagcgc ccagaacgca
      181 aaaagagcgc gtgagctaca gaagactatg gatgcgcagc agaaacgcct acagagcgtc
      241 acagagcctt ttaaggggct ttccagagag cagatgaccg aaatcctgat gatggctcag
      301 cggtttaagc agcaaaacca ggaaaaggaa aaacagcagc gtattgaacg tgaaaaacag
      361 cgacagacgc gatcgcgtgg catgggtggt atggagcgtt agttttgatg cgatgcagga
      421 tagggctaac gccgcaaagt gacgtcattt gcggcattcc agattaggtt ctgccgccct
      481 gtataacgct gaggccgttt tttagtgctt tcttgtggtt gctgacagta cctttccagc
      541 caaactgagg gttcagacgg taactgtaac tgcgccctac ttttgggcct tgaagaatca
      601 cggataggtc aattagcgcc ttaaccgaac ggcttacatt ggttttctgc atacctaaag
      661 cgtcggcaat atcggcttgg gcaatttgga tgtaattttc ataatcgaga tcagctaaca
      721 gcatcatcaa gactttgaat tgctcccctg tgagggtctt agcaaggatc tttaaagcgt
      781 cttggttcat agtgaaatgc ctctcaaaag ctgacttctg tttgggtcgg ataactgcga
      841 caaaactacc tacctcttcc cctgtagcag tgtctatctg agtaattcgg ttactcattt
      901 aagaggctct cccaaaaaaa tagttatcaa taatgataac aacgttatca ataatgataa
      961 ctatcgtcaa gcgagagccg cgccccacaa gggctgagag aaggctctct tctgtatctt
     1021 atagcgagaa gctcatatgc ccggatagcg aaccctattt tcatcggttc gtgcgtaagc
     1081 atttctatgc ccgttcaggt gatattccgc ttcctcgttc aggctgttcg ttcctcgctg
     1141 cgctgcggta cgcctcagcc tgacctgcgg cgagcggcgc gtgctctttc aaagacgact
     1201 ttgttgcctg acattcgtca agggcgagcg gagcgagtgc atttcaccct tgacgacgca
     1261 caaataaaac aggcttggca gtggtgggag gtgacgcgga gcgacgcctt ccacctgcca
     1321 gaccgccgtt gattttgacc ttctggcagc ttgctgcccg aacgattttg cgtagcaaag
     1381 tctagtgagt acactatcat cttgatagtg gcccgccgga ggtatcgcaa tgcgctcccc
     1441 cgcctggctg aatgaatgcc ggaatcaggg gggaacatgg cgaactacgc gataatgcgc
     1501 tgcaaaaagc tgacgggaat gggaagcgtg gccagcgcgt tgcagcactg ctaccgagag
     1561 cgtgagacgc cgaacgctaa cgctgaacgg acaccagaaa actactgctc tgtgtccaaa
     1621 tcaacggatc aggcaatggg gcgtgtgcgg gagctgctgc cggaaaaacg ccgtaaagat
     1681 gcggtactgg ccgttgaata cgttatgaca gcaagccctg agtggtggaa agaggccaca
     1741 ccacggcagc aggcggagtt tttcgcccgc tctgagcaat ggctggagaa gaaatacggg
     1801 aaggatcgtg ttgtggcggc ggtggttcac cgggacgagg ccaccccgca cctgtctgcg
     1861 ttcgtggtgc cattgacgca ggatggacgg ctgtccgcga aagagtttat tggcgggcgg
     1921 tcaaagatgc gtgaagatca gagcacctac gctgaatcag taaaaaaact gggtcttgag
     1981 cgcggcatcg agggaagccg cgccacgcat cagacagttc agcactacta cgaatcgata
     2041 aac
//
