LOCUS       NZ_AP027803             9396 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.10.01CC plasmid p2017.10.01CC_3,
            complete sequence.
ACCESSION   NZ_AP027803
VERSION     NZ_AP027803.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562596
            Assembly: GCF_036504915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 39x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036504915.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:02:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,739
            CDSs (total)                      :: 4,619
            Genes (coding)                    :: 4,396
            CDSs (with protein)               :: 4,396
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 223
            CDSs (without protein)            :: 223
            Pseudo Genes (ambiguous residues) :: 0 of 223
            Pseudo Genes (frameshifted)       :: 50 of 223
            Pseudo Genes (incomplete)         :: 166 of 223
            Pseudo Genes (internal stop)      :: 30 of 223
            Pseudo Genes (multiple problems)  :: 23 of 223
            Pseudo Genes (short protein)      :: 4 of 223
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027803.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.10.01CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.10.01CC_3"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(join(8444..9396,1..163))
                     /gene="mobA"
                     /locus_tag="AABJ86_RS23610"
                     /old_locus_tag="VEE56_45390"
     CDS             complement(join(8444..9396,1..163))
                     /gene="mobA"
                     /locus_tag="AABJ86_RS23610"
                     /old_locus_tag="VEE56_45390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_023281056.1"
                     /translation="MASYHLSVRTGGKGNASPHADYISREGKYARKKDADLEHKEAGNM
                     PAWAAHNPSEFWKAADAFERANGCTYREIEIALPRELNPAQRLELVRAFVSQEIGDRHA
                     YQFAIHNPTAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKVPERGGAKKVRFG
                     ETPTERKEHLIAQRERWADLQNAYLERYQHADRVDARSLKAQGIGREPERHLGAGQVQR
                     FDTDQLQAILERREAERQVQQCCDERDSVIDVTTSLREAISERDTLMLKQTQKSDPEQD
                     AVSGRVFDFEKEPEKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQEIHQEMERQKERAR
                     LAEKQRQQEKERQRIAEQKRQKPDKGWSFSR"
                     /name="mobA"
     gene            327..779
                     /gene="traD"
                     /locus_tag="AABJ86_RS23615"
                     /old_locus_tag="VEE56_45330"
     CDS             327..779
                     /gene="traD"
                     /locus_tag="AABJ86_RS23615"
                     /old_locus_tag="VEE56_45330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_023281057.1"
                     /translation="MSKSEKQEKWVADRVQYIRGLKSPNEQQRLMVLLTDKVNRTAQDI
                     KTLSLLIQAEKAAEKAQEARTKVMNLIQAEKRAEARAARKARDHALYQSAGLLIMAGLV
                     DSQTGKPVDDAAALLGALASLNDLSRDNPKWSDWKIRGQELLNSRK"
                     /name="traD"
     gene            1395..2372
                     /locus_tag="AABJ86_RS23620"
                     /old_locus_tag="VEE56_45340"
     CDS             1395..2372
                     /locus_tag="AABJ86_RS23620"
                     /old_locus_tag="VEE56_45340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_047402701.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPSLETPDETE
                     RTVKFSCSEYASFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLDHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISIRIDEFKFMLMLENSYPAYKLLNHWVLKPISKDLNSYSNMKLAIEKRGRPADTLI
                     FQVELDKQIDLVTELAKEDPATAPEKTTRTPIPVTNSTQDSQLHNGLKKTLHDALTAKI
                     QLTSFEAKFLSDMQSKHDLNDSFTWLTQKQRATLEKILAKYGRF"
                     /name="replication initiation protein"
     gene            2380..2829
                     /locus_tag="AABJ86_RS23625"
                     /old_locus_tag="VEE56_45350"
     CDS             2380..2829
                     /locus_tag="AABJ86_RS23625"
                     /old_locus_tag="VEE56_45350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023281052.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSSGSSKLDTSE
                     LLRVFGSFTGQQLGSEQSGSTVQSLAGESVQLTDQNLLHENYALKAEIELVKALLREKE
                     LLLAEKDKRNEDLKQALLLIESKLPATPEPVTQPMVKKSWKFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            2808..2957
                     /locus_tag="AABJ86_RS23630"
     CDS             2808..2957
                     /locus_tag="AABJ86_RS23630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152930637.1"
                     /translation="MEILEKIEVSARLLAEIISVVLITNRQHKNSQFWGQKTGCFARFD
                     VFSS"
                     /name="hypothetical protein"
     gene            3115..5163
                     /locus_tag="AABJ86_RS23635"
                     /old_locus_tag="VEE56_45360"
     CDS             3115..5163
                     /locus_tag="AABJ86_RS23635"
                     /old_locus_tag="VEE56_45360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966094.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_023281053.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKAL
                     VANEKIRAVYGTDIDGIFIFDFDNLCKFLKYKEYWANSFTKYRNKVGLTSEGKYLDYSS
                     DVVLDFPFKDCVLEGGMTKENQGKDEIYYNEIIARDEIDRLFSPKVFTNVKRHTKNGAQ
                     NNISYFDQSDNLIIKGNNLIALNSLITPHKGEVKLIYIDPPYNTGSDSFKYNDRFNHST
                     WLTFMKNRLETSLKLLSDDGFIFIQCDDNELFHLKVLMDNIFGVENFINIITIKTKIGG
                     VSGSSEGKSLRDATEFIILYSKRKELAMLNPVFKKTEVIQYVNEYKDSGKSWKYTQILL
                     DLGAKTPIKSEDGVTYNHYPYAKTTSISRFSKEKGISENDVYKDYAGIVYRTTNAQSSV
                     RGRVLKELEEINNGIVSVEYTPLKGKNKGTPVEIFYNASNKDMFMFLSDMLEKNGESYN
                     YMQKINTLWDDIQYNNLNKEGGYVSFENGKKPELLIKRVIELSTKAGDLILDFHLGSGT
                     TAAVAHKMGRRYIGIEQMDYINDITVPRLQKVIGGEQGGISKDINWQGGGSFVYAELME
                     LNAYFVHEIQKAQSTEELEKLFAVMKTEAHLNYQVALENVLSAEYEVDGIFRKVAFSEL
                     ELHEQKQLLIEILDKNQLYVNASDMDDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            5168..7804
                     /locus_tag="AABJ86_RS23640"
                     /old_locus_tag="VEE56_45370"
     CDS             5168..7804
                     /locus_tag="AABJ86_RS23640"
                     /old_locus_tag="VEE56_45370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443154.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_023281054.1"
                     /translation="MAKKPTSAGSEKLLFNKLKQFDEPGLFHDNYQTPDYIQANLKDAL
                     RPYQHGALRYLHYTQRKREEALLHYRHLLFHMATGAGKTMVMAGTILYLFKEYGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSQELEIDGEKITIAPVETFPSVPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEEQTWE
                     RTIENILDLRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDGDKMLDAVLLSQYRKLTAADNGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESVRRHLANKRVQLSSDTSIWHKVIQRYADSDLVTVIQQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLENIDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTGTDSEAQL
                     IGRGARYYPFVYDGKRSFTRRFDNSAKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGSGTIEHARLKEDFKKSTVYQTGKLYFNKVEEIESSSRRWETYSLETRFEIPYQT
                     AGEESLDNLTGATAVITKPEPLVLDERFYRKAMQRISFYALDNLQRFFPKLTGIREFIR
                     SDAYLGKLKITVTVPQSLDFSSVPAKEKLHLLETVLLRISENIRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVVINQKITTAPTIGKKWYVYDNAILNQLEHRLVKTLEAFMPKL
                     KARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNTYWQVFLEPKGDDRL
                     LDDAWKEQMLETLNDRERIVIDENEDVRLVGIKFFANSQMDAFVSDMQNRLNEGESLET
                     ASFSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(7847..8431)
                     /locus_tag="AABJ86_RS23645"
                     /old_locus_tag="VEE56_45380"
     CDS             complement(7847..8431)
                     /locus_tag="AABJ86_RS23645"
                     /old_locus_tag="VEE56_45380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_023281055.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVSTLTEEFQAASVQLQRQSETLSK
                     VIRELDSASGNMTDTVRKSVSSAMNQVTQALTQAGTEQQKPAVNALNQVVKAANDSVNA
                     MRREMYRYTWKSAIYLVLTIFFVLACCVTALTWFMNDGYSRIAEMQRMEAVWQKKAPLA
                     DISTCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     Typing          1672..2372
                     /name="rep_cluster_1787"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8448..9394)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.177"
                     /coverage="99.0"
                     /evalue="9.82e-169"
ORIGIN
        1 cagacggatt atgcgccgcc catgccggca tgttgccggc ctccttgtgt tcaaggtcgg
       61 cgtctttttt gcgggcatac tttccctcac gcgaaatgta atcggcatgg ggggaagcgt
      121 tacctttccc gccggtcctg acgctgagat gataggaggc cattgtgttt tatcccgcgg
      181 aaggcgcgca ccgtttctga acaaagtgaa gaaacgtcta agtgcgccct gataaataaa
      241 aacttatcag ggattgtagt ggaatttgac ctttgcagct accagtgagc ataattccac
      301 cgttaaaacg cagaggacag acgggtatga gtaaaagcga aaaacaggaa aaatgggtgg
      361 cagacagagt gcagtatatt cgcggtttaa agtcgccgaa tgaacaacag cgcctgatgg
      421 tattgctgac ggataaagtg aacaggacag cgcaggatat caaaacactg tcactgctga
      481 ttcaggcaga aaaggccgca gagaaagcac aggaagccag aacaaaggtc atgaacctga
      541 tacaggcaga gaaacgggct gaagccagag ccgcccgtaa agcccgtgac catgcgctgt
      601 accagtcagc cggattactc atcatggcgg gtctggtaga cagtcagacg ggaaaaccgg
      661 tcgatgatgc ggctgctctg ctgggcgcat tagccagtct gaatgacctg tcacgggata
      721 atccaaagtg gtcagactgg aaaatcaggg ggcaggaact gctgaacagc aggaagtgag
      781 acaggaacac ctgagcagat agcgattaaa cgccctgaga agcccgtgac gggctttttt
      841 tgttttatgg gtggaatcct tcactgtaac ccatgaaaga cgtctgtagc gccatttaac
      901 cccatttcct gccagagccg tgagcgaagc gaactgattt tcacgaaaac aaccgcgatt
      961 cgggtgccgg atggtcggag acgaaagaaa tgttcagcga gttgcccgag cttacgaagg
     1021 gagagctacc tcagtgtttc ggggttaaga gatctgtttt gaagaggagc aaacagcgtt
     1081 tgcgacatcc tgttatcaat actgaacaat tgtctctggt ggtcagttat ccacagggct
     1141 gggatctatt cttttttatc tttttttatt ctttttttat tctaaggatt ttaagttatt
     1201 gttataaaac aaaaaaaaac gcaaaaggtt aagcaaaaat taggaaaggt taagcaaaaa
     1261 ttaggggtcc cggccaaaag gttaagtgaa aattaggtaa tccacacaac ctccaaaaag
     1321 gttatgccag tgcaattgac ataaccaatg gtaaaggtta tgttatttta aaatcacaac
     1381 caatggtttt agtcatggct gaattggtag tattcaaagc aaatgagctt gcagttagtc
     1441 gttatgacct aacagagcac gaaacgaaac taatcctgtt ttgtgttgca aagctaaacc
     1501 cctcattaga aacaccagat gaaactgaac gaacggttaa attttcatgc tcagaatatg
     1561 cttcatttat ggggataagt tatgagaatg cgtgggggcg attaaataca tcaacacgag
     1621 aattatttaa gcgctctatt gaattaatat atccaacagg tgctgtatct atgcgcgttt
     1681 ttaattgggc tgaatatgca gagttcaata gggaaacaca agaggtaact ttagttttta
     1741 gtaaatacat aatcccattg ctatttcacc taaagagatt cattaagtac aacctcgatc
     1801 atgttaagtc ttttgaaaac aaatactcaa tgcgcattta tgaatggctg ctaaaagaac
     1861 taacacaaag aaaaacacat aaagccaata tttcaataag aattgatgaa tttaaattca
     1921 tgttgatgtt agaaaatagc tacccagcat ataaattact aaaccattgg gttttaaaac
     1981 caatctcaaa agatttaaat tcctacagca acatgaagtt agcaatagaa aaacgaggcc
     2041 gtcctgctga caccctgatc tttcaggttg agttagacaa acagattgat ctagtaactg
     2101 agttagccaa agaagatccc gccacagcac cagaaaaaac aacaagaacg cccattccgg
     2161 tgacaaacag cacgcaagac agtcaattgc ataacggtct taaaaagaca ctacacgatg
     2221 cactgaccgc aaaaatccag ctcactagct ttgaggctaa atttctaagc gatatgcaaa
     2281 gcaagcacga tctcaacgac tcatttacat ggctgaccca aaaacaacga gccacgctag
     2341 agaaaatttt agctaaatac ggacggttct gaggtatcta tggcactggt atcaatcagc
     2401 gaagccgcaa gactgacagg aaaaagtcgc acaacagtgc agtcatacat taagcaggga
     2461 aaactgacca agtgcacaga tagcagtggt tcttcaaagc tagatacatc agagcttctg
     2521 cgtgttttcg gatcatttac tggtcagcaa cttggcagtg aacaatcagg cagcactgtg
     2581 cagagtttgg caggagaatc cgtacagtta actgaccaga atttgctgca tgaaaactat
     2641 gctttaaaag ctgaaataga actcgtaaag gcattgctgc gtgaaaaaga actcctactc
     2701 gctgaaaaag acaagcgcaa cgaggatctc aagcaagccc tgttactgat agagtccaag
     2761 ctaccggcaa caccagaacc tgtcacgcag ccgatggtga aaaaatcatg gaaattctgg
     2821 aaaaaataga ggtttcagcc cgtttgctgg ctgaaatcat atccgtcgtt ttgataacaa
     2881 accggcaaca caaaaacagc caattttggg ggcagaaaac aggctgtttt gcgcgttttg
     2941 acgtgttttc gtcgtgagta cctgatcggt ggtgcgcgta agagagttta tgttaaatac
     3001 cacttattct ggtgcatttt cccgcaactg aattcaactc atcgctgtcg ggttccagcg
     3061 ccggacgcta tatcttacaa tcattcagat attaaacagt cataaggtca tgacatgcag
     3121 acagaattaa taaaagaact taacaaggtt ttaaaagcat tcccgcagtt ctggaacgga
     3181 gaggaattac aacgttcaat ggtgagtgac gctatcagtc aaaagcagcc agaccttatc
     3241 aaggcattgg tggcgaatga aaaaatccgc gctgtttacg gtacggatat agacggaatt
     3301 tttatttttg attttgataa tttatgcaaa tttcttaaat acaaagaata ctgggcgaac
     3361 agcttcacta aataccggaa caaggtggga ttaaccagtg aagggaaata ccttgattac
     3421 agctctgatg tggtgctgga ctttcctttt aaggattgtg ttcttgaagg cggaatgacc
     3481 aaagaaaatc aggggaaaga tgagatttat tacaatgaaa tcattgctcg tgatgagatt
     3541 gatcgacttt tctctccaaa ggtattcact aatgttaaac gacatacaaa aaatggtgca
     3601 caaaacaata tatcttactt tgatcaaagt gataatttaa tcatcaaagg aaataactta
     3661 attgccctta attcattaat aaccccccat aaaggcgagg taaagttaat atatattgac
     3721 cctccatata acactggttc cgatagcttt aaatataatg atagatttaa ccactccacg
     3781 tggttaacat tcatgaaaaa taggcttgaa acatcactta aattattatc agatgatggg
     3841 tttattttta tacaatgtga tgataacgag ttattccatt tgaaggtgtt aatggataac
     3901 atatttggtg tagaaaactt catcaatatt ataacaataa aaacaaagat aggtggtgta
     3961 tctggaagct cggaaggaaa aagcttaaga gatgcaactg aatttataat tttatattcg
     4021 aaaagaaaag aacttgccat gcttaaccct gttttcaaaa aaacagaggt aattcagtat
     4081 gtaaatgaat acaaggatag cggtaagagc tggaaataca ctcaaatact cttagattta
     4141 ggtgcaaaaa cgcccataaa aagtgaagat ggagttactt acaatcacta cccctatgcc
     4201 aaaaccacct ccatatctag attttcaaaa gaaaaaggca taagtgaaaa cgacgtatat
     4261 aaagactatg cagggattgt ttatagaaca acaaatgcac aatccagtgt cagaggccga
     4321 gttctaaaag aactagaaga aataaataat ggcattgtta gcgtagagta cacaccatta
     4381 aaagggaaaa acaaaggaac gccagttgaa atattctaca acgccagcaa taaagatatg
     4441 tttatgttcc tatccgacat gctggagaaa aatggtgaaa gttataacta catgcagaaa
     4501 ataaatacct tatgggatga catccaatat aacaatttaa ataaagaagg tggatatgta
     4561 agtttcgaaa acggaaagaa accagagttg ttaattaaaa gggtcataga gctatctaca
     4621 aaggcaggcg atttaatctt agactttcat ttaggtagcg gaacaaccgc agcagtagcc
     4681 cataagatgg ggcgtcgcta cattggtatt gaacagatgg attacatcaa cgacataacg
     4741 gtaccacgcc tgcaaaaagt catcggaggt gaacaggggg ggatctcaaa agacatcaac
     4801 tggcagggtg gcggcagctt tgtctacgct gaactgatgg agctgaacgc ctactttgtg
     4861 catgaaatac agaaagcgca gtccactgaa gaactcgaaa agctctttgc cgtgatgaaa
     4921 acagaggcac atctcaacta tcaggtggca ctggagaacg tgctgtcggc tgagtacgaa
     4981 gtggatggta ttttccgtaa agtcgccttc agcgaactgg aactgcatga gcagaagcaa
     5041 ctgctgattg aaatcctcga taaaaatcag ctctatgtga acgcttccga tatggatgac
     5101 agtgacctga acatttcaga gagcgataaa gcctttaccc gcagcttcta cggaatggag
     5161 tgacggcatg gcgaagaaac cgacctcagc cggcagcgag aagctgctgt ttaacaagct
     5221 gaagcaattt gatgaaccgg ggctgttcca cgataactac cagacgccgg actacattca
     5281 ggcgaatctg aaggatgccc tgcgtccgta tcagcacggt gcactgcgtt acctgcatta
     5341 cacgcagcgt aaacgtgaag aggcgctgct gcattaccgt catctgctgt ttcacatggc
     5401 aaccggtgca gggaaaacga tggtgatggc ggggacgata ctctatctgt ttaaagagta
     5461 cgggtatcag aatttcatct tcttcgtgca taccgacgcg attatccaga aaacccgtga
     5521 gaacctgctg aatccgcagt cgccgaagta cctgttcagt caggagctgg aaatcgacgg
     5581 ggaaaaaatc accatcgcac cggtggagac gttcccgtcc gtaccggaac gcaacacgat
     5641 ttaccttaag ctctcaacca tccataagat gcacgatgag ctgaacagct atcgtgagaa
     5701 cagcatcacc tacgaggatt tgaaggaaat tccgcttgtg ctgcttggtg acgaggcgca
     5761 tcacttcaac gccggcacca aagccagagg aaagtcgaaa acctcaccgg agaacgagga
     5821 gcagacctgg gaacgcacga ttgagaacat cctcgacctg cgaccggata accggctgtt
     5881 cgagttcacc gccaccattg acctggcaaa taaagatatc gggcaaaaat accgtgacaa
     5941 agtggtttat cagtatgacc tgaagcagtt catgtcagac ggctattcga agaaagtcat
     6001 gctgctggaa gccaaccaga atgacggtga caagatgctc gatgcggtac tgttaagcca
     6061 gtaccgtaaa ctgacagcag cagataacgg catcaccggc ttcaagccgg tcattctgtt
     6121 caagtcgaac aagattgcga tatcaaaggc gaagcaggag gagttttcgc aactgattgc
     6181 ggcaatgacg cctgagagcg tcagacgcca cctggcgaac aaaagagtac agctcagctc
     6241 tgacaccagt atctggcaca aagtgattca gcgttacgct gacagcgatt tagtgacggt
     6301 tatccaacag attcaggaag actttaacga cttcaacctg ctgaacgtca acaagtcaga
     6361 cctgctggaa gaaaaccccg tcctgctgaa cacactggaa aacatcgata atccggtgag
     6421 agccgtcttt gccgtggcga aggtcaacga aggctgggac gtgctcaacc tctacgacat
     6481 tgtccgcatc agcgagcagg cgagcagcag caaaaccggc acggacagcg aggcgcagct
     6541 tatcgggcgt ggtgcgcgtt actacccgtt cgtttatgac ggtaagcgca gcttcacccg
     6601 ccggtttgat aacagcgcga aagatttgag tgtgctggag caactgcact atcacaccat
     6661 caatgaacca gcttatataa aaacgctgca cgcatcatta gagcaggcgg acattgacgt
     6721 gcatcaggat ggcagcggca cgattgaaca cgccagactg aaagaagact tcaagaaatc
     6781 cacggtctat cagacgggta agctgtattt caacaaagtg gaagagattg agagcagcag
     6841 ccggcgctgg gaaacctact cgctggaaac acgctttgag ataccgtatc agaccgccgg
     6901 tgaggagtcg ctggacaacc tgacgggcgc aacggcagtc ataacaaaac cggaacccct
     6961 ggtactggac gagcgttttt accgcaaggc gatgcagagg atcagcttct acgcgctgga
     7021 taacctgcaa cgcttcttcc cgaaactgac cggcatccgt gagtttattc gcagtgacgc
     7081 ctatcttggc aagctaaaaa tcaccgtcac cgtaccgcag tcactggatt tctcctctgt
     7141 accggcaaaa gagaaactgc acctgctgga aactgtgctg ctgcgcatct cagagaacat
     7201 acgccgcaat gaccagaagg tgaaaggcac gtaccgcttt atcagccagc cggtgaagga
     7261 ggtgattaaa gactacagcc tgcacattga cccgtcggtg gtcattaacc agaaaatcac
     7321 caccgcaccg accatcggta aaaaatggta cgtgtacgat aacgcgatac tgaaccagct
     7381 tgagcatcgc cttgtgaaaa cgctggaagc attcatgccg aagctgaaag cgcgttacga
     7441 cgacatttac gtactgcgta acgacgagca gtcaacgcgc tttaaactca cggagttcgg
     7501 cggcgtgcgc gggtttatgc cggatttcat catgatactg acgcgccatt cggacaacac
     7561 ttactggcag gtgttccttg agccaaaggg ggatgaccgg ctgctggatg acgcatggaa
     7621 agagcagatg ctggaaacgc tgaatgacag ggagcgcatt gtgattgatg aaaatgagga
     7681 cgtcaggctg gtcgggatta aattctttgc caacagtcag atggatgcct tcgtcagcga
     7741 catgcagaac aggctgaatg agggagaatc gctcgaaacc gcctcgttct ccctgccgtt
     7801 gtaggtcgca acaacaccca atagcggagc gtaatgctcc gttattttat ttcttaataa
     7861 tcatccaggt attctcttta tccccatacg tggtgcgggt gtccacctta acgcagggct
     7921 ttccgtcaca cgtagagata tcagccagcg gcgctttttt ctgccagaca gcttccatac
     7981 gctgcatttc agcaatccgg ctgtacccgt cattcataaa ccatgtcagt gccgtcacac
     8041 agcaagccag gacgaagaat atcgtcagca caagataaat cgccgatttc cacgtatagc
     8101 gatacatttc acggcgcatg gcgttaacgg agtcattagc tgctttcacg acctgattaa
     8161 gggcattcac ggcgggcttc tgctgctcag tgccggcttg tgttaacgcc tgcgtaacct
     8221 gattcatagc ggagctgaca gactttctga ctgtatcggt catattaccg gacgcactgt
     8281 ccagctcccg gataaccttg ctcagtgttt cgctctgccg ctgtaactgc acggaggcag
     8341 cctgaaactc ctcagtcagc gtgctcactc gtttttccag cgcctgatga tgcccgataa
     8401 gggcagcaat ctgtttaatt tcgtcgctca tatcaaatcc tgcttatcgt gagaatgacc
     8461 agcctttatc gggcttctgt cgtttctgtt cggcaatgcg ctgccgctct ttctcctgct
     8521 gacgctgttt ttccgccagt ctcgcccgct ctttctgccg ttccatttcc tgatgtatct
     8581 cctgaaactc cgccatcgca tcgttaacaa gggactgaag gtcaatttct tcctgtatgt
     8641 ccttcatggc atcgctgacc agtgcattca gcttctccgg ctctttttca aaatcgaata
     8701 ccctgccgga aacggcgtcc tgttcgggat cagatttctg cgtctgtttt agcatcagag
     8761 tgtcccgctc actgatggct tcccgcagcg aggtggttac gtcaatcacg ctgtcacgtt
     8821 catcacagca ctgctgaacc tgtctttccg cttccctgcg ctcaagaatg gcctgtaact
     8881 ggtcagtatc gaagcgctga acctgtcccg cccccagatg ccgctccggt tcacgcccga
     8941 tgccctgcgc tttcagggag cgggcatcga cacggtcagc atgctgatag cgttcaagat
     9001 aggcattctg gaggtcagcc cagcgttcac gctgggcgat aagatgctct ttgcgctctg
     9061 tcggtgtttc cccgaaacgc acctttttag ctccgccgcg ctccggtact ttagagtttg
     9121 cccgtttgaa atactgctgc gggtcacggt caatcccgtc attcatccgt tcagaaaaca
     9181 taatatgcgc gtgcggctgt tcgccgccgg caatcgccgc tgtcgggtta tggatggcaa
     9241 actgatacgc atgacggtcg ccgatttcct ggctgacgaa cgcacggacg agttcaagac
     9301 gctgtgccgg attaagctca cgcggcaggg caatttcaat ctctcgatag gtgcagccgt
     9361 tggcgcgttc aaaggcgtct gccgccttcc agaatt
//
