LOCUS       NZ_AP027795             7559 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.06.05CC plasmid p2017.06.05CC_5,
            complete sequence.
ACCESSION   NZ_AP027795
VERSION     NZ_AP027795.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562594
            Assembly: GCF_036504765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 183x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036504765.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:25:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,191
            CDSs (total)                      :: 5,071
            Genes (coding)                    :: 4,814
            CDSs (with protein)               :: 4,814
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 73 of 257
            Pseudo Genes (incomplete)         :: 187 of 257
            Pseudo Genes (internal stop)      :: 44 of 257
            Pseudo Genes (multiple problems)  :: 45 of 257
            Pseudo Genes (short protein)      :: 2 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027795.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.06.05CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.06.05CC_5"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(join(7470..7559,1..105))
                     /locus_tag="AABK28_RS25830"
     CDS             complement(join(7470..7559,1..105))
                     /locus_tag="AABK28_RS25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041629.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELGNSIQTHFEAQYGTEP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            723..917
                     /locus_tag="AABK28_RS25835"
                     /old_locus_tag="VEE55_49140"
     CDS             723..917
                     /locus_tag="AABK28_RS25835"
                     /old_locus_tag="VEE55_49140"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_116835902.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCRKTGRF"
                     /name="ANR family transcriptional regulator"
     gene            complement(3047..3466)
                     /locus_tag="AABK28_RS25840"
                     /old_locus_tag="VEE55_49150"
     CDS             complement(3047..3466)
                     /locus_tag="AABK28_RS25840"
                     /old_locus_tag="VEE55_49150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_094315649.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3588..3866
                     /locus_tag="AABK28_RS25845"
                     /old_locus_tag="VEE55_49160"
     CDS             3588..3866
                     /locus_tag="AABK28_RS25845"
                     /old_locus_tag="VEE55_49160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315650.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQEAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3858..4148)
                     /locus_tag="AABK28_RS25850"
                     /old_locus_tag="VEE55_49170"
     CDS             complement(3858..4148)
                     /locus_tag="AABK28_RS25850"
                     /old_locus_tag="VEE55_49170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315653.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTSPSA"
                     /name="hypothetical protein"
     gene            4389..6035
                     /gene="cka"
                     /locus_tag="AABK28_RS25855"
                     /old_locus_tag="VEE55_49180"
     CDS             4389..6035
                     /gene="cka"
                     /locus_tag="AABK28_RS25855"
                     /old_locus_tag="VEE55_49180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(6032..6322)
                     /gene="cki"
                     /locus_tag="AABK28_RS25860"
     CDS             complement(6032..6322)
                     /gene="cki"
                     /locus_tag="AABK28_RS25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            6352..6483
                     /locus_tag="AABK28_RS25865"
     CDS             6352..6483
                     /locus_tag="AABK28_RS25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6678..6821)
                     /locus_tag="AABK28_RS25870"
     CDS             complement(6678..6821)
                     /locus_tag="AABK28_RS25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     Typing          complement(1726..1804)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="87.0"
                     /evalue="1.57e-30"
     Typing          7303..7390
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          complement(6750..7292)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.157"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcctgttcg tctgcatcca gtgcatccag tttttcgagc aacgtcaggc tctggctttt
       61 gataaatcgc gccatgttga gcgcggtctg ctgctgtttg ttcacgttct cccttctccg
      121 ttactggtgt ttacgtccgg ctgtgcgtca cctggtcatc acgccaggtg acgtcactga
      181 aaattatcac acaagccccg tcgtgtgtgg gctgcgcgac attaacaccc cgtgacgctt
      241 attttttgct gaatatgtgc gcgattttct gtctgcgttg tgtgcagttg tcgtcataaa
      301 catgaacgtt ccgccggttc ctgatgtgcg tcactttgac gttttgacaa agtgacgcac
      361 gggaggtgct tgcgcaaacc cgcgaataac ggcgtttacc gcgtgatttt tttacatctg
      421 acaacgtgac ccggcgtgat ggccagttct ggccagagac gttcgcgggg tgtcggggcg
      481 cagccatgac cgggtggcgt ttgtccggtc gtgtgtgcgc ggtccgacaa atgcacatcc
      541 tgtcccgttt ttcacacggt ttttattgat taccggacag acgaacccgg attgccatat
      601 tgcgaaaatc acgttaaaac gattacgagc gtgcagttgg gctgatatat caacagtcat
      661 gccaatatgt attaacccga caggccgctc tttttaattc caataaaaat ataaatacga
      721 gtatgaatca ggacaaagca atttatttag aaaataaata tttttcaccc gcaaacgaag
      781 ctgtcagact ggagcgtgaa ggcgattaca ttggagcatg gaaaaaatgg aatgatgcgc
      841 ttcattatgc caaaggagaa aatataggtt ggtgtatagc cagagctgaa tattgtagaa
      901 agacaggccg gttttaattc tggcggggtt tcaaagggga gtcccctttg gcacgtcgac
      961 gtatcaaaat gcgccagcat tttgtataca gtcggtcacg tgcctccaga atttaccctc
     1021 aaaacgcctc tgtaactgac gcataacaaa agtataacca agatataatt aacatataac
     1081 caaggtataa ctaacctggt gtgtatataa ttaagatata attaatatat aactaacggt
     1141 ttaccaacgg atcgcatgta gtagacgcta cataaaaaat cctgaatatg gaggattcgt
     1201 cataaagaga ttcctgtatt taccgttctg atgcaggaca gggcaaacac tgcaaaaatg
     1261 atgaaaaact gattaaccat actgtctgta ttctgaatga attacgaagc atagcgataa
     1321 attaaaaaat ccccctggat tacaggggga agccatcaga gtttgagtaa tgagaaatat
     1381 tattctggag agagcaccac aaatgcgctc ttttccaatc tgccgtaacc atcggcaaaa
     1441 gacaatcagg acaggcgaaa aaatccccct atatggcaag ggggtgtcaa tgaggacatg
     1501 gcaattgtga gttttgtgtg tgttttatgt gttgcagttt gcaacgtcag aaatttgccg
     1561 tagttcgtca tgagagacaa caaaaaaatc ccccgtgact ggtggaggcc agggcaaggg
     1621 gggtagcaaa tgcctgaata attttcgtag ttttgatata cccatcgttg caaccgcaac
     1681 ggggtgaatt tgccgcagtt gtgcgcgaaa aacaatcctg attccgggac aggatgtgta
     1741 atcgtataac cgcgcacgca cggctatgcg attaccacct ggtcagggct ttgccccgac
     1801 accctcgaca ccgttcccgc gatacgatag aacatcaggg cgcgtttccg ggtaaacgca
     1861 tgttaatgac cgggaattca ttgatcaggg accggagacg cgctacaaaa ctcctgcgcg
     1921 tttctggcat caactggcca tggggatggt ttggcagtag cagcaagcgc cagaaacgcg
     1981 ctcaaaacgt ctctgacagc ctgaacccac caaaacatat aaaataattg cccgatgcaa
     2041 aaaagtgcgt tagaaagcgt ctgagcgcgt ttccttgcca cctgaacgtc atctctggga
     2101 ttcgtgctca ctccatcaaa taacaaataa acttcaaagt ctgataatgg gttccccctg
     2161 taatccaggg gatttttttc gctcactcat taccgcacaa ctcgacatca cgccggagac
     2221 acgctaaaac actcctgcgc gtttctggcg tcttttttgt tgctgtgtcg ctatactcgt
     2281 tgtcagattc gcgctcaatc atcactgatt aatctgcgtt tatccttcga ccgatcacgc
     2341 tggcattttt tgacaaccgg cgcccatgaa aacgccagac gggaaccgtc atccgcaccc
     2401 tctgacatac tggggattaa aaatccctgt gccgcacagc cggcgttgca taaaattccg
     2461 ctctgcggtg agtattgttt aatcaaaaat ccccgcagtt cttccggaaa aacgcgagtc
     2521 tgttctcatg cctgaaatgc ccacacacta cgcaaaacca actttttgct gatttgcttt
     2581 ttaaatcatg tcgttatggc tctaaaaata tattttctta ttcagattta tacaaaagca
     2641 cgtcgtcgac gaccctatgg cgacgctatg acgaccctgc ttagggtcag tactgactat
     2701 ctgtagaaaa aaacagttaa ttaatgtagg ggcggcccta ggggcgtgtc gcattttttt
     2761 acagagcgat cctaggggcg ctcctaggat cgttctggtc tgatatgcag caatgagtca
     2821 gagaattacg aactgaaaat ttacgaaaat aagcctcagg gacagcggac gcgcccaaac
     2881 cgatcgccat tcagtttcat gcatagtcat gcaacgcgct gaaaacgttc ctgacgcatt
     2941 tttcctgttt accccgggga aaacatccct ttttacggtt tttgcgccag aaacgcgctg
     3001 agcgcgtttt aacggtgcgt acaattaagg gattatggta aatcccttac tgtctgccct
     3061 cgtatccatc gagataaacc gcagaaatgg tgtctgccat tagtctggat tttttcgggg
     3121 taaggtcttt gctgcggtaa tcagtacccg caaattgagt tgtgccccgg agtgagttta
     3181 aaaattcctg gctgtgaaac tgctgaatgc gttgttgcac atcactccgt gattttcctg
     3241 tcgctttatc tttagctccc atatcccaga actgccgaaa aaaaggcacc atcatacgta
     3301 tggtgtctgc tttctgggtg tagaagttag gatcaaatcc atcatctgaa gcataaacat
     3361 aatgccgggt atgtcgttcc aggctggtct ttggtgtgca tcctgtaaga gctattgttg
     3421 ctgttatcag aggtatcaga agcagcctgg cagcggcaga tctcataaac atctccttgc
     3481 gtttttaatc ttgatactca gggagttata tctccgaaga gtatctctta tcatacgtcg
     3541 gtttactccc aatgtgattt gatggtcaat gagtcaggaa taatcccgtg aaaaaatgtg
     3601 tgccagtcct gctttttagt gttctttctg cttcggttgc cagtcatgcg gacagcaatg
     3661 tttttcaaca agaggccgag aagacaaaaa acggcattgt tgcggatgtt aatgccagac
     3721 agaaaatgtt cagtaacaga ctgaagatac aacttcagat cgatgagttg agagcagagc
     3781 tggctggcac cagagatgcc caaaaaagag aggatttgcg taaaaaactg gagtctctgg
     3841 agcggcaaaa gaaatcctta cgctgatggg gacgtatttt ccccggtttc ttccataacc
     3901 tttttaccgt tcagatattt acgggcgagg cgaatgcgtc ttgcctggta taccgccgag
     3961 taagcctggg tgggattcac accattactg atttcactga ccacatgacg ctgatctgcc
     4021 agtgtcagcg tgtaaacctg tttctggatg aggtcacggg ctttgtcatg ctcatatccg
     4081 gcagcaacca gggtgtcgag aagtcgcttg tctgttttgt tcagtttatg gcggggagat
     4141 ttgctcacat cgttctccgt tttttcatga tccaacagtt ttatatctca tcctgatttg
     4201 ctccggtgtt ctgtttcgac gttctcaatg tctgcctcct tgtagtgtga tctttattgc
     4261 aaaaatgaaa atccatgttc ttgacatggg caatgctgag tagtaggttt ccactgtaca
     4321 taaaaccagt ggttatatat acagtacgtt gtttttaatt tattgtttta attatcaaag
     4381 aggaatttat ggctaaagaa ctaagtggat atggaccaac tgctggtgag tctatggggg
     4441 gaacaggggc gaatctgaat cagcaaggtg gaaataataa tagtaatagt ggggttcact
     4501 ggggtggtgg ctctgggcat ggtaataatg gtggtcaggg aaattcaaat tcatctggtt
     4561 ctacatcaac tgtgatgaaa accggtgagt catatctgac tccgtggggt gatgttgtca
     4621 ttaataatga tggccttccg gtgatgaatg gtattgttat gacagaagag aattcaactc
     4681 tggttgataa tccatttgga ggggtctcca gagttcttaa ttctttaatt agtgatatgc
     4741 catcactttt tgcagaaagc tcaggaaata acaacaataa cactgcttca gttaatactg
     4801 caccaacgaa tgctcaggtc agtgatatgg ataagagttc gaaggtggtg ggtaatgtaa
     4861 ttaatgaaaa acaaaagcag aagaataaaa ttgctacaca gattagtgaa aaacagaaga
     4921 aaatcgagga gatgaaaaaa gtattcaagc atcattctta tcatggtatc actgatcttg
     4981 agagggatgt tgatgagttg caaaaaaaat caaatcagct tgatgcagat attagcaaac
     5041 ttaattctta taaaaataca ttgcaaagta aaattggtga tgtgaataaa cagaaggaag
     5101 ctgaagaaaa ggctcgtgaa aatgcagagg tcgctgaaca tgaaactctt aatgaagaga
     5161 aacaggcggt ggcggaagca gagaagcgtt tagctgaggc gaaagctgaa ctggcgaagg
     5221 ctgaaagtga tgtacagagt aagcaagcta ctgtttccag agttgcagga gagcttgaaa
     5281 acgctcagaa gagtgttgac gtgaaggtca ccggatttcc gggatggcgt gacgttcaga
     5341 aaaaactaca gcgtcagctt gaagctaagc aggctgagta ctccgcagtt gaaaatgaac
     5401 ttaagaatgc tgttagtttc agggatggta aggccgctga ggttaaagag gcagagcaga
     5461 aattaaaaga agctcaggat gctcttgaaa agagccagat aaaagatgct gttgatacta
     5521 tggttggatt ttatcaatat ataaccgaac aatatggaga aaaatacgca aaaattgctc
     5581 aggatttagc ggaaaagtct aaaggaaaga aaattcaagg tgttgatgaa gctctggcag
     5641 catttgaaaa gtataagaat gtgctggata agaaattcag taaggttgat cgggatgcta
     5701 tttttaatgc gttagagtct gttaattatg atgagttatc taagaattta acgaagatat
     5761 caaagtcgct taaaatcacc agtcgagtat cttttctata tgatgttggt tctgatttta
     5821 aaaatgcaat agaaactggt aactggcgac ctttgtttgt cacgctggag aaaagtgcgg
     5881 tcgatgtagg tgtggcaaaa attgtggcat taatgtttag ttttattgtt ggtgtgcctc
     5941 ttggtttctg ggggattgca attgtcacag gtattgtttc ttcttacata ggggatgatg
     6001 agttgagcaa acttaatgag ttattaggga tttaatttct ctttagagtg taatatatga
     6061 aacttggcaa tcctactggg attgccatta ataaataaaa caatgcagat aacttgcata
     6121 agtaccaggg ttcttttttt aactttgtat tttctttgat atatctagat atggttaatt
     6181 tagagtatgg atatagcaat acatgtattg atgatataaa tataaataac aaaggagtgt
     6241 tatcattgtc gttaaatatt aaaataagaa tccatggtat aagtgattca ggtaaattat
     6301 gtaggtagta ttttaaatgc ataagtattt ttacttttgt tggaaagaaa tatgaaaata
     6361 ataatttttc taattacaat attattcttg tcaggatgtc aagcaaacta tattcgtgat
     6421 gtacaaggag gaaccgttgc accatcttcg tcttctgaac tgacggggat cgcggttcag
     6481 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcctgt
     6541 aaacgaaaaa accgcctggg gaggcggttt agtcgaaggt taagtcagtt ggggactgct
     6601 taaccgggta actggcttta gtggagcaca gataccaaat gctgtccttc cagtgtagcc
     6661 gctataaggc ttcaacttca agagttctgc gtatctaaat ccactatcca gttaccaatg
     6721 gctgctgcca gtggcgttaa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6841 acgacctaca ccgaactgag ataccaacag cgtgagctat gagaaagcgc cacgcttccc
     6901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6961 agggagcttc cggggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaagcgc
     7081 ctccggcgtg gcctttttcg gggttttcgt ttcttgctta catcccctga ttctgtggat
     7141 aacactatca ctgcttttga gtgggcagat accgctcgcc gcagccgaac gaccgagcgt
     7201 agcgagtcag tgagcgagga agcggaaaag cgcctggacg agcattttct ccttacgcat
     7261 ctgtgcggca tttcacaccc ggcatggtgc acttttcata caacccgcac tgatgccata
     7321 tgattaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     7381 acccgctaaa acccgctgat gcgccctgac gagcttgtct gctggcgaaa acactcacgg
     7441 acaagccgcg accgctttcg ccagcccctt tatggttccg tgccgtactg cgcttcaaag
     7501 tgtgtctgta tgctgttacc gagttcttcc gccagttcgt gcagtcgttc gcacatggc
//
