LOCUS       NZ_AP027787             6647 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017-03-3CC plasmid p2017-03-3CC_4, complete
            sequence.
ACCESSION   NZ_AP027787
VERSION     NZ_AP027787.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562624
            Assembly: GCF_036504395.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 546x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036504395.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:31:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,288
            CDSs (total)                      :: 5,161
            Genes (coding)                    :: 4,820
            CDSs (with protein)               :: 4,820
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 341
            CDSs (without protein)            :: 341
            Pseudo Genes (ambiguous residues) :: 0 of 341
            Pseudo Genes (frameshifted)       :: 102 of 341
            Pseudo Genes (incomplete)         :: 253 of 341
            Pseudo Genes (internal stop)      :: 49 of 341
            Pseudo Genes (multiple problems)  :: 57 of 341
            Pseudo Genes (short protein)      :: 3 of 341
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027787.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017-03-3CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017-03-3CC_4"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            130..267
                     /locus_tag="AABK30_RS26395"
     CDS             130..267
                     /locus_tag="AABK30_RS26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(466..624)
                     /locus_tag="AABK30_RS26400"
     CDS             complement(466..624)
                     /locus_tag="AABK30_RS26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(1294..1485)
                     /locus_tag="AABK30_RS26405"
                     /old_locus_tag="VEE74_50630"
     CDS             complement(1294..1485)
                     /locus_tag="AABK30_RS26405"
                     /old_locus_tag="VEE74_50630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1549..1872
                     /locus_tag="AABK30_RS26410"
                     /old_locus_tag="VEE74_50640"
     CDS             1549..1872
                     /locus_tag="AABK30_RS26410"
                     /old_locus_tag="VEE74_50640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2081..>2512
                     /locus_tag="AABK30_RS26415"
                     /pseudo=""
     CDS             2081..>2512
                     /locus_tag="AABK30_RS26415"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2550..3068
                     /locus_tag="AABK30_RS26420"
                     /old_locus_tag="VEE74_50650"
     CDS             2550..3068
                     /locus_tag="AABK30_RS26420"
                     /old_locus_tag="VEE74_50650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3075..3308
                     /locus_tag="AABK30_RS26425"
                     /old_locus_tag="VEE74_50660"
     CDS             3075..3308
                     /locus_tag="AABK30_RS26425"
                     /old_locus_tag="VEE74_50660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3626..4045)
                     /locus_tag="AABK30_RS26430"
                     /old_locus_tag="VEE74_50670"
     CDS             complement(3626..4045)
                     /locus_tag="AABK30_RS26430"
                     /old_locus_tag="VEE74_50670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4126..4563)
                     /locus_tag="AABK30_RS26435"
                     /old_locus_tag="VEE74_50680"
     CDS             complement(4126..4563)
                     /locus_tag="AABK30_RS26435"
                     /old_locus_tag="VEE74_50680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4823..6391
                     /gene="cea"
                     /locus_tag="AABK30_RS26440"
                     /old_locus_tag="VEE74_50690"
     CDS             4823..6391
                     /gene="cea"
                     /locus_tag="AABK30_RS26440"
                     /old_locus_tag="VEE74_50690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     Typing          1122..1209
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(553..1108)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1863..3412
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttttttgt tataaggtat atatatataa gtgtgcagta caggccaaat aaaatatttt
       61 ttatgtagta tcttaagctc ataaattaaa cctcgccata tattcttttc attttataag
      121 gatcgagtta tgaggaaaag attttttgtg ggaatattcg cgataaacct ccttgttgga
      181 tgtcaggcta actatatacg tgatgttcag ggagggacca tcgcaccatc ctcctcttct
      241 aaactgacgg ggatcgcggt tcagtagaaa agatcaaagg atcttcttga gatccttttt
      301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccaacg gtggtttgtt
      361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      421 taccaaatac tgtccttcta gtgtagccgt agtcgggcca ctacttcaag aactctgtag
      481 caccgtttgt gccatcatcg ctctgctaat ccggttacca gtggctgctg ccagtggcgt
      541 taaggcgtgc cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      661 gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      781 aaacgcctgg tgtctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtctatt
      841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gcctgctacg cggccttctt
      901 cctgtttctg gtcttttgct cacatgttct ttccggcctt atcccctgat tctgtggata
      961 actgtgttac cgtttttgtg tgagtcagta ccgctcgccg cagtcgaacg accgagcgta
     1021 gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgt gcattttctc cttacgcatc
     1081 tgtgcggcat ttcacacccg gcatggcgta cttttcatac aatccgcact gatgccgcat
     1141 ggttaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctg cgccccgaca
     1201 cccgctaaaa cctgctgacg cgccctgacg ggcttgtcag ctcccggcat ccgctcacag
     1261 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catccccgaa
     1321 acgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg tgaagcgatt cacaaatatc
     1381 ggcctgttca tctgcgtcca gttcgttgag cttctccagc agcgttaatg tctggcttct
     1441 gataaagcgg gccatgttaa gggcggtttt ttcctgttta gtcactgatg cctccgtgta
     1501 agggggattt ctgttcatgg ggtaatgata ccgatgaaac gcgagaggat gctcacaata
     1561 cgggttactg atgatgaaca tgcccggtta cttgaacgct gtgagggtaa acaactggcg
     1621 gtatggatgc ggcgggtctg cctgggggag ccggttgccc gttccggaaa actgccgaca
     1681 ctggcaccgc cgttactgcg tcagctggcc gccatcggaa ataacctgaa tcagacagcc
     1741 cgtaaggtga acagcgggca gtggtcttcc ggtgaccggg ttcaggtggt ggccgcactg
     1801 atggccatcg gggatgagct gcgccggctg cgtctggccg tcagggaaca gggggcgcgg
     1861 gatgatagtt aaatttcatg ccaggggaaa aggtggtggc agtggtccgg ttgattacct
     1921 gctggggagg gagcgtaacc gcgaaggcgc aacggtgctt cagggtaatc cggaagaagt
     1981 ccgggaactc atcgatgcca cgccatttgc gaagaaatac acgtccggtg ttctgtcgtt
     2041 cgcggagaag gagctgccgc cgggaggacg tgaaaaagtg atggcgagct ttgagcgtgt
     2101 actgatgccc ggtctcgaaa aaaatcagta cagcatcctg tgggtggagc accaggacaa
     2161 gggacggctt gagctgaatt ttgtcattcc gaacatggag ctacagaccg gaaaacgcct
     2221 ccagccgtac tacgaccgcg cagacaggcc tagaattgat gcctggcaga cgctggtgaa
     2281 tcaccattac gggctgcatg acccgaacgc cccggagaac cgcaggacgc tgacactccc
     2341 tgataacctg cctgaaacga aacaggcgct tgctgagggc gtcacgcgag gtatagatgc
     2401 actttaccat gccggagaga taaaaggccg tcaggatgtg attcaggcgc tcactgaggc
     2461 ggggctggaa gtggtcaggg tgacgcgaag cagtatcagc attgcagatc cgaacggcgg
     2521 gaagaatatc aggctgaaag gagcatttta tgagcaatct tttgcagacg ggcgcggagt
     2581 tcgagaaaaa gctgaaagag agagccgaat ctacagagaa aatgctgaac aacgagttca
     2641 ggaggctcgg cgaatctgta agcgaggctg tgacatcaaa cgagacgaaa atcagagacg
     2701 ctatagccct gttcacagcc tcgacagagg aatcgctgga aaaacaccgg gaaggggtga
     2761 aagaggcgat gatgcagcac aggagggacg tgttaaagct ggcagggaat acgggcatga
     2821 tgttactggg gatagtcttt ctcctgttta ccgcgagtgg cgggacgctc tggtatcttg
     2881 gagggaggat acaggcgaac ctggaagaaa tcaggaagca ggaagagaca ttgcagaaac
     2941 tgaacgcgaa gacatggggc gtggagtttg tgcaggacgg gaacaggaaa ttccttgtcc
     3001 ttccgtacgg gaaatcagcg gaggtgattc cctttcaggg gaaagagtgg gtacatctga
     3061 aggagtgaca cagagtgaca gagctggaaa cacatttgct gaacgcctta gagcagctgc
     3121 aacaggacta tatgcagcgg ctgagcgaat gggagagcgc cttcgtggaa ttgcagaaga
     3181 tgttttcgct tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg cagttgagtc
     3241 agcaggtgca gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaact
     3301 ggagataaga gaggaacggc tgatacagga gcgcgaacat gtgttatccc tggaacggga
     3361 gcgtcagccg gaaatacagg aacgcacgct ggatggccct tcgctgggat ggtgaaacca
     3421 tgaaaaatgg cagcttcagt ggattaagtg ggggtaatgt ggcctgtacc ctctggttgc
     3481 ataggtattc atacggttaa aatttatcag gcgcgatcgc ggcagttttt cgggtggttt
     3541 gttgccattt ttacctgtct gctgccgtga tcgcgctgaa cgcgttttag cggtgcgtac
     3601 aattaaggga ttatggtaaa tccacttact gtctgccctc gtagccatcg agataaaccg
     3661 cagaaatcgt gtcagccagc agcctggatt ttttcggggt aaggtctttg ctgcggtaat
     3721 cagtacccgc aaattgagtt gtgccccgga gtgagtttaa aaattcctgg ctgtgaaact
     3781 gctgaatgcg ttgttgcaca tcactccgtg attttcctgt cgctttatct ttagctccca
     3841 tatcccagaa ctgccgaaaa aaaggcacca tcattcgtgt tgtgtcggct ttttgggtgg
     3901 aaaagttggg atcaaaaccg tcatcagaag catatacgta atgccgggta tgttgttcca
     3961 cgctgccctt tggggtacag gctgtgagag ctactgttgc tgttatcatg gcgataagac
     4021 aaagtttgtt tccggtacgt ctcatgagtg tctccctacc ttaaagtatt tattctcggt
     4081 gagttattat ccgaagacta ttttatcatc agtctctgac agcgttcagc gaccgaacaa
     4141 cctggaccag aatccccgtt tcttttccag ttccagtgcc tgcctgagct gggctatctc
     4201 attttgtagc tgttcccgtt ctgcttcctg gcgtctgcga tccatatcct gtgcctgttt
     4261 atcctcaagc atcagcgtca ggcattgttt cagctcattc agctcccgga gaatgcgttc
     4321 tgtctgctga tcatgtggat tttctgcatg tccctcactg tgcctttctg gtgtctcatt
     4381 ctgctttaat tcgccgtatg cccggatcag ttcactggtt tcaaactccc gtcgtccgtc
     4441 cctgccggta cggtaactga caaggcctgc gcgcatatca cggtaaaact gtgaacgcga
     4501 tctgcctgtc atttttagtg cgtcccggag tgtgtgccat gccataaagt gacagtgtcc
     4561 catagatgtc tcatctcata gtttcagtaa aacataatga ggtctgagaa cggtaatgtt
     4621 tgtgctggtt tttgtggcat cgggcgagaa tagcgcgtgg tgtgaaagac tgtttttttg
     4681 atcgttttca caaaaatgga agtccacagt cttgacaggg aaaatgcagc ggcgtagctt
     4741 ttatgctgta tataaaacca gtggttatat gtacagtatt tatttttaac ttattgtttt
     4801 aaaagtcaaa gaggatttta taatggaaac cgcggtagcg tactataaag atggtgttcc
     4861 ttatgatgat aagggacagg taattattac tcttttgaat ggtactcctg acgggagtgg
     4921 ctctggcggc ggaggtggaa aaggaggcag taaaagtgaa agttctgcag ctattcatgc
     4981 aactgctaaa tggtctactg ctcaattaaa gaaaacacag gcagagcagg ctgcccgggc
     5041 aaaagctgca gcggaagcac aggcgaaagc aaaggcaaac agggatgcgc tgactcagcg
     5101 cctgaaggat atcgtgaatg aggctcttcg tcacaatgcc tcacgtacgc cttcagcaac
     5161 agagcttgct catgctaata atgcagctat gcaggcggaa gcagagcgtt tgcgccttgc
     5221 gaaagcagaa gaaaaagccc gtaaagaagc ggaagcagca gaaaaggctt ttcaggaagc
     5281 agaacaacga cgtaaagaga ttgaacggga gaaggctgaa acagaacgcc agttgaaact
     5341 ggctgaagct gaagagaaac gactggctgc attgagtgaa gaagctaaag ctgttgagat
     5401 cgcccaaaaa aaactttctg ctgcacaatc tgaagtggtg aaaatggatg gagagattaa
     5461 gactctcaat tctcgtttaa gctccagtat ccatgcccgt gatgcagaaa tgaaaacgct
     5521 cgctggaaaa cgaaatgaac tggctcaggc atccgctaaa tataaagaac tggatgagct
     5581 ggtcaaaaaa ctatcaccaa gagccaatga tccgcttcag aaccgtcctt tttttgaagc
     5641 aaccagacga cgggttgggg ccggtaagat tagagaagaa aaacaaaaac aggtaacagc
     5701 atcagaaaca cgtattaacc ggataaatgc tgatataact cagatccaga aggctatttc
     5761 tcaggtcagt aataatcgta atgccggtat cgctcgtgtt catgaagctg aagaaaattt
     5821 gaaaaaagca cagaataatc tccttaattc acagattaag gatgctgttg atgcaacagt
     5881 tagcttttat caaacgctga ctgaaaaata tggtgaaaaa tattcgaaaa tggcacagga
     5941 acttgctgat aagtctaaag gtaagaaaat cggcaatgtg aatgaagctc tcgctgcttt
     6001 tgaaaaatac aaggatgttt taaataagaa attcagcaaa gccgatcgtg atgctatttt
     6061 taatgcgttg gcatcggtga agtatgatga ctgggctaaa catttagatc agtttgccaa
     6121 gtacttgaag attacggggc atgtttcttt tggatatgat gtggtatctg atatcctaaa
     6181 aattaaggat acaggtgact ggaagccact atttcttaca ttagagaaga aagctgcaga
     6241 tgcaggggtg agttatgttg ttgctttact ttttagcttg cttgctggaa ctacattagg
     6301 tatttggggt attgctattg ttacaggaat tctatgctcc tatattgata agaataaact
     6361 taatactata aatgaggtgt tagggattta attattcttt attgatataa aaagtcctag
     6421 caatccaaat gggattgcta ggaccaaaca aagtagatta tatagcataa ataggtttaa
     6481 ttttgctacg ggggcgttat ttaggttttt tcttctttcg aaaaaatctt tctttatgaa
     6541 gttaaaagct atgtgttcaa tagcatattt tgaatatgga catagaatag tgcttatcac
     6601 tattgcatat agcatcttat ctgacacaag gaaataatac ccctcgc
//
