LOCUS       NZ_AP027780            10900 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.03.04CC plasmid p2017.03.04CC_5,
            complete sequence.
ACCESSION   NZ_AP027780
VERSION     NZ_AP027780.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562590
            Assembly: GCF_036504195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 83x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036504195.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:15:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,598
            CDSs (total)                      :: 4,486
            Genes (coding)                    :: 4,081
            CDSs (with protein)               :: 4,081
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 405
            CDSs (without protein)            :: 405
            Pseudo Genes (ambiguous residues) :: 0 of 405
            Pseudo Genes (frameshifted)       :: 170 of 405
            Pseudo Genes (incomplete)         :: 225 of 405
            Pseudo Genes (internal stop)      :: 49 of 405
            Pseudo Genes (multiple problems)  :: 37 of 405
            Pseudo Genes (short protein)      :: 4 of 405
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027780.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.03.04CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.03.04CC_5"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            join(9951..10900,1..346)
                     /locus_tag="AABJ94_RS22900"
     CDS             join(9951..10900,1..346)
                     /locus_tag="AABJ94_RS22900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="WP_247754857.1"
                     /translation="MSNHEENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKIGVDDCEPTSFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="virulence-associated E family protein"
     gene            complement(1069..1536)
                     /locus_tag="AABJ94_RS22905"
                     /old_locus_tag="VEE53_45860"
     CDS             complement(1069..1536)
                     /locus_tag="AABJ94_RS22905"
                     /old_locus_tag="VEE53_45860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_101998987.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRDWAKARA
                     DAPSNVLSIEEMRERAQRAVEATPQAAVRRLKNHFVQRQYELLQRHASDLQALRSASLQ
                     AMLEKDATMVKAISGLMTSLLKAQERERLIYEIKPGTEADIMRQGMNRHQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2095..2682)
                     /locus_tag="AABJ94_RS22910"
                     /old_locus_tag="VEE53_45880"
     CDS             complement(2095..2682)
                     /locus_tag="AABJ94_RS22910"
                     /old_locus_tag="VEE53_45880"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181213545.1"
                     /translation="MNKTYGYTRVSTREQTADGQRDAIAAHYPDALVLGEEPMSGTVPP
                     RERPTLGILLNQILQEGDTLVVAAIDRIGRNARDTLELVEEMEKRKINLISLREGVELN
                     TATGKSVIGVVASFAEMERTLILERQAAGIAAAKKRGQKFGRPTKIGATEVGEIVTRRA
                     MGVPIASIAKQLKLSRSAIYRCLEKIDAKAPE"
                     /name="recombinase family protein"
     gene            complement(2694..3269)
                     /locus_tag="AABJ94_RS22915"
                     /old_locus_tag="VEE53_45890"
     CDS             complement(2694..3269)
                     /locus_tag="AABJ94_RS22915"
                     /old_locus_tag="VEE53_45890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151785.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_101998997.1"
                     /translation="MSRVFGYGRVSTGEQTVAGQKKAILDRYPQATFHADEGISGTVPA
                     TQRDKFKAILEFAAPGDSIVVVAIDRIGRNARSVMEVCEELEKRQVSLISLREGFDFST
                     PTGKMMLTMVAAFAEMELATLKERTKAGMAAAAAAGRHAGRPTKQTPENVGAVIMKHDA
                     GVSAARIARDLGISRGTVNRILQKHAKA"
                     /name="recombinase family protein"
     gene            3520..4179
                     /locus_tag="AABJ94_RS22920"
                     /old_locus_tag="VEE53_45900"
     CDS             3520..4179
                     /locus_tag="AABJ94_RS22920"
                     /old_locus_tag="VEE53_45900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_336474967.1"
                     /translation="MTEIKKESADGAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRLDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAETDGGRGLYRTMIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            4362..4751
                     /locus_tag="AABJ94_RS22925"
                     /old_locus_tag="VEE53_45910"
     CDS             4362..4751
                     /locus_tag="AABJ94_RS22925"
                     /old_locus_tag="VEE53_45910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_338390039.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSTAARGIDWNA"
                     /name="hypothetical protein"
     gene            4744..5106
                     /locus_tag="AABJ94_RS22930"
                     /old_locus_tag="VEE53_45920"
     CDS             4744..5106
                     /locus_tag="AABJ94_RS22930"
                     /old_locus_tag="VEE53_45920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_308821875.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGAGDE"
                     /name="hypothetical protein"
     gene            complement(5304..5636)
                     /locus_tag="AABJ94_RS22935"
                     /old_locus_tag="VEE53_45930"
     CDS             complement(5304..5636)
                     /locus_tag="AABJ94_RS22935"
                     /old_locus_tag="VEE53_45930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_308821876.1"
                     /translation="MKRTLVALALIATSITAHAAPNSQRFDCSLIDGTHLDMVLYPDDT
                     VTLKIPGNRVVVPLTLEETNRDDRSISADAYASNGYKFAQIVFGHNLAHILLPNTEGLC
                     KAIDKL"
                     /name="hypothetical protein"
     gene            complement(5695..6459)
                     /locus_tag="AABJ94_RS22940"
                     /old_locus_tag="VEE53_45940"
     CDS             complement(5695..6459)
                     /locus_tag="AABJ94_RS22940"
                     /old_locus_tag="VEE53_45940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_268646976.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGDTQT
                     TYSVTYAYGQPKDKWHPEKVDVSMGKVKIPRK"
                     /name="hypothetical protein"
     gene            6857..7066
                     /locus_tag="AABJ94_RS22945"
     CDS             6857..7066
                     /locus_tag="AABJ94_RS22945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_148502023.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPMLKRFIADQRRPGRATDGVR"
                     /name="hypothetical protein"
     gene            7053..8219
                     /locus_tag="AABJ94_RS22950"
                     /old_locus_tag="VEE53_45950"
     CDS             7053..8219
                     /locus_tag="AABJ94_RS22950"
                     /old_locus_tag="VEE53_45950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174599769.1"
                     /translation="MAFDNSHLADPGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSARHNRGEAKHRSQYRHFLKLKGITNPEAMAQVFLQFSGVEWNRQASVQ
                     SDADRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALAAA
                     GGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCSAE
                     GFQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLSRG
                     KVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPVPTIPG
                     AAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            8476..8934
                     /locus_tag="AABJ94_RS22955"
                     /old_locus_tag="VEE53_45960"
     CDS             8476..8934
                     /locus_tag="AABJ94_RS22955"
                     /old_locus_tag="VEE53_45960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174599770.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPRQ
                     QTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     IVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(8952..9440)
                     /locus_tag="AABJ94_RS22960"
                     /old_locus_tag="VEE53_45970"
     CDS             complement(8952..9440)
                     /locus_tag="AABJ94_RS22960"
                     /old_locus_tag="VEE53_45970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186838.1"
                     /translation="MREKTRTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKDARQSAPQTARQSAPQNARQIVPRGADPRFQ
                     AVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYRHKLRERGLI"
                     /name="hypothetical protein"
ORIGIN
        1 gcagctgtgg gctgaggcgc tccacctgtt caatgagaat ggggtgatgt gggaggccgc
       61 tgaaacgctg gcgcgtaagg tgcatgacga gttccgcgtc gaggatccgt gggaagacga
      121 gatcgccgag ctgctcaaga tcggggtcga cgactgcgaa ccgaccagct tcgtgccgac
      181 taaggcgttg ttctctcagg tagggctcat gattggccat accagcaaca agcagcatca
      241 gcgcatggcg gctatcatgt cccgattggg ctatgaggtt ggcaggaagc gcataaacgg
      301 tgcgttagtt cgtggctact tccgtcgaac gcaagctgtc gtgtaagggt tacttttccg
      361 taagagggga gctatatgct cccctttttt attgttccac ccgggtggat cacgggtgga
      421 tcatcacgcc gtttttgtac cacctgttcc acccgggtgt tccacccgtt ccacctgtgt
      481 tccgcccgtg gatggaacag ctaaggccta gtgtttaaag gctttcgttg ttgtgttcca
      541 cccgttccac cctattttct aattaaaaac aaaaaatgaa taaattgggt aaataggggg
      601 taaatgcttg ctaattgaac aattagttgt tttttttact atttatgaat ctatgccgga
      661 tcgggtggaa cgggtggatc atcttggaat tgacgcgccg cagctgcacc ctgcgggatg
      721 cactttgctt gtcttggtcg atcggtggat gggttcgtcg tgtcggggtg gtgggtggtg
      781 gtggttaggg ggtggatcgg gtggttgggg tggcgctgag ggcacgagct gtcgctcgcg
      841 ctgggtggtc gcttggtggt tcagcgtgag ggcttgccct cgcgccctct gcgttatggg
      901 tggaatgcgt agctgcagct gcgcgtttgg ttcgtccggg catcaaaatg gggcttctcg
      961 cgcacgcgag cggtaacaat tgcgtaacgt gcggatccgc gtattccggg ggctcccggg
     1021 ttttctgcgg cggtaaaacg gcggtaaaag gcggctttcc cgccgttttc agctctggtg
     1081 gcggttcatg ccctgccgca tgatgtcggc ctcggtgcct ggcttgatct cgtagatgag
     1141 gcgctcgcgt tcctgcgctt taagcaggga ggtcatgagg ccgctgatcg ctttgaccat
     1201 ggtggcgtcc ttctccagca tggcctgtag cgatgcgctg cgtagggctt gcaggtcgct
     1261 cgcgtggcgc tggagtagct cgtactggcg ctgcacaaag tggtttttga ggcgtcggac
     1321 tgcggcctgt ggggtggcct cgacggctcg ctgtgcgcgt tcgcgcatct cttcgatgct
     1381 gaggacgttg ctgggggcgt cggcgcgggc tttcgcccaa tctctgacgg tgcggggtgg
     1441 gatccccatg gtgcgtgcga cggtggcact ggttgcgccg tcctgctcca gtagcctgag
     1501 cgcctgggct ttctgttcgg tggtgtattt cttcatggtt cctgctccgg ttggtttctc
     1561 tcccgtattg aaacgtcacg cgctgaaaat ggcgggggtt tttggggggt acacggcgtt
     1621 tgtgcgtgcg tggtcgtgcg gttgggtgtt tttgtggctg aggcgcttag aatggctctg
     1681 gtgcgttctg gtgaaggggg cgcggaagcg cccctgtaag tggttcgtaa tggcgtaagg
     1741 cttttgttat tgcttgctgc ttacacctgt aaggctttcg ttatggtcat gcctcccatg
     1801 cgtaaaggct gatgatgctg acggtgcggt atcgccctgc ctggtcgcgg agggtagtct
     1861 ggtactggcc tcggcggttg gcggcgcaat gacgcggctt gccaaactgg cggatctcgc
     1921 cgtgttcgat gctcaggccc gggtagcggg caggggtggt ctgttgtatg ctctgcatgt
     1981 cattaaacct ctaacgggtg gttggtgatg cccgggtcgg ctctcatact ttcccgggcc
     2041 tgggtgcggc ggtaccattc gagtcaccat aactcggtac cgctgcgctc tctcttactc
     2101 cggcgctttc gcgtcgatct tctcaagaca ccggtaaatt gcgctccggc ttagttttag
     2161 ctgcttggct atgctggcga tcggcactcc catggcgcgt cgcgtcacaa tttcgccgac
     2221 ttccgtggct ccgatcttgg tcgggcgtcc gaacttctgg cctcgctttt tcgctgcggc
     2281 gatccctgct gcctggcgtt cgaggatgag tgttctctcc atttctgcga agctggccac
     2341 cacgcctatt accgactttc ctgttgcggt gtttagctcc accccctcgc gtaggctgat
     2401 gaggttgatc ttccgcttct ccatctcctc gaccagctcg agggtgtccc gggcgttcct
     2461 gccgatgcgg tcgatcgccg caactaccag ggtgtcgccc tcctgcagga tctggttaag
     2521 gaggatgcca agggtcggac gctcgcgtgg tggtaccgtc ccgctcattg gttcttcccc
     2581 aaggacgagg gcgtcggggt agtgcgcggc gatggcgtcc cgctgcccgt cggcggtctg
     2641 ttccctggtt gagacacggg tgtagccgta tgttttgttc acttttcgct ctcttacgct
     2701 ttcgcgtgct tctgtaaaat cctgttgacc gttcccctgc tgatgccgag gtcgcgtgcg
     2761 atgcgtgcgg cgcttactcc tgcgtcgtgc ttcatgatca ccgctccgac gttctccggc
     2821 gtctgtttgg tcggtcgtcc tgcatggcgt cctgcggctg cggctgctgc catccctgcc
     2881 tttgtgcgct ccttgagggt ggcgagttcc atctctgcga aggcagcgac catggtgagc
     2941 atcatcttcc cggtcggggt gctgaagtcg aaaccttcgc ggaggctgat caggctgacc
     3001 tgccgcttct ccagctcctc gcagacctcc atgaccgatc tggcgttgcg tccgatgcgg
     3061 tcgatcgcga ccaccacgat gctgtcgcct ggtgcggcaa actccaggat ggctttgaac
     3121 ttgtcgcgct gggtggcggg taccgttccg ctgatgccct cgtcggcgtg gaaggtggct
     3181 tgtgggtagc gatcgaggat cgctttcttc tgtccggcta ctgtctgttc tccggtgctg
     3241 acgcgcccgt acccgaatac tctgctcatg ctgtctcctg ttgtacaaaa aaggttgttt
     3301 ggggatattt gaccacttat gagcaaaaag caagcgcaaa aaactgtaaa cgcctagagc
     3361 cacgggggct gcgcagtttc gctccttttt ggacgtgatc accccgcgct gggcgagagc
     3421 cctgcgccct ctgcgtgggt gtgctcggtt ggtcgtgacg gcatgctgtc tgagcggcgt
     3481 ttcttgccgc tttgctgata acttgctgag aacctaacca tgaccgaaat taagaaagag
     3541 agcgccgacg gcgcttggct acgctctacc cttgccgaat tggcgggtga tgcgttccct
     3601 gtgtatgacc tcccgtccct gcaggtagac acgaactcct ccattcagct ctcggcgctc
     3661 gccgatcgtc aggctgcgcg cgaaatgcga caggccgctt ccgacgtgga agcgcgacgg
     3721 ctggacgccg ctcgagtggt ggaagggctc aagaccgagg ccgaacggct gcggggtttg
     3781 atcgccgacg gcaaggctgc gctgcgagcc ggtgagccgg tgagccccga cgcgggtgtt
     3841 gcgtcgttcc tgctgccgga catcgaggcc gagctggttg ttgctgaggc tgctgaggcc
     3901 gacgtggccc gggagcgtga caccctgctg caggatgccg atcgccttga cgcggcggct
     3961 gcgctggcgc tgttcaactg ggcgcactcc gttcgcgtgc agcgtatcga gctgcttctc
     4021 aagctggcga tggacgaggc gacgacgctc gctgaaaccg acggcgggcg cggcctgtac
     4081 cgtaccatga tcgcgcctga ccgccgtctt aaccaaattt tcgccacgca gggcgctatt
     4141 gaaatcctgc gccgcaaccg tggcatggag ggtgtgtaat gtctgctttc aaactcctgt
     4201 gcgaccggct tcgcaatgag gcgacgctgg caaaatctga cggggccggc gctttcaaaa
     4261 agcaatctct ggatcccgac ctgaagggcg gcaagaaggg caaaaagccc gctggggagc
     4321 tggaggagga cgaggcgatc gcgtctctcc accgtaagcg catgaacgac gttaaaggtt
     4381 gcaagacgct gaccaaatcc accggcgctg atgactttgg cctccctccg ctgtcgtcgg
     4441 tggtcgagac agtggtggag acaggcggcg cagcgcagac gttcgcgccg atcggggctc
     4501 cggtggcgtc tccgaccgaa gcggttcacc gtgcgcgtgc gcagtccatc caggacgcgg
     4561 cgttcctgaa cgagatgaaa gcggcgggtg tggcaccggc tgacctggtg ttacttgacg
     4621 gcgtgttgac gtcctctcgt gggatgccag acgcggcgcg tgctccggcg tttgccgagg
     4681 tgcgccgtca ggttcaggcg ggcggcatga cgctgtcaac ggctgcgcgt ggtattgact
     4741 ggaatgcgta agctgccgcg tcacctgcag ggcgaggtgt tgatgccgct gtccagcagg
     4801 atcacgcgct cgcaacggct ggagctgatc tggctgttgc ccgttctgcg gctccacccg
     4861 ggcgatcgat cggtcactga gcggcgggcg cttggcaggt tccggggatc gctcgctgcg
     4921 ggtggccaga cggatgcttt cgtccgttcg gatgcgtctc tgtacgcgct gggactgcgg
     4981 ttccagcgca agcgcacccg ggagctggtt gcttcggatc ccgatgttgc tgcggctggc
     5041 gaggcgctgg agcagcgtgc aaacggcgtg aacctgtccg agacgttcgg ggctggcgat
     5101 gagtgaattt gccgtctcgc gtctggttgt ccctgagatt gcgatcccgg ctggggttgc
     5161 tgctgcccga cgggcaaggg gacaggcaca acgtatcgca gctgctcgcc gtgctgcaga
     5221 gcgtcgttcc gcgcctacgt ggcgctggtg gcgacgagga ttgctgctgc tgcgagtgct
     5281 ttcatcattt tgttgttgtt ccgttaaagt ttatcgatgg ctttgcacaa tccttccgtg
     5341 ttcgggagga ggatgtgtgc gaggttgtga ccgaatacaa tttgggcgaa cttgtaaccg
     5401 ttggatgcgt aggcgtctgc cgagatcgag cgatcgtcgc ggttcgtctc ctcaagggtg
     5461 aggggaacga ccacccggtt gccggggatc ttgagcgtca cggtgtcgtc gggataaaga
     5521 accatgtcga ggtgggtacc atcgatcagg ctgcagtcaa accgctgact gttcggtgcc
     5581 gcatgggcgg tgatgctggt ggcgataagc gccagggcta ccagtgtgcg tttcatgttg
     5641 ggtactcctc tgttgcttga ccggacatta agcctacgtc taaaacgact caagctattt
     5701 cctcgggatt ttcaccttgc ccatgctgac gtccaccttc tccgggtgcc atttgtcctt
     5761 cggctgtccg taggcgtagg tcacgctgta agtggtttgc gtatcgccgt cgccaccgtt
     5821 ccagcgcagg ggctcgcggt gttcgtggca gtagtacacg aaaccgtcgc ggcgtgtttg
     5881 cccgaccggg cctggtcggc gatcgtggaa ccgtgcgccc gggttggctc gcatggcgcg
     5941 gacgagcgcc tcgccctggt ggatgccgta ccagacctgc cagccatcgc cgatgccgat
     6001 caggaacggc gagacgaacg gtggggtgcg gttgactctc ccgcgtgcgt tggctccgat
     6061 ggtggcttcg cgttcgcggt tccagcagga gacgcacagg ctcgaaccgg gcttattggg
     6121 ggcgatcagg cggttgtctt tccggctgca acgggggcag agcttacggg tgtctgccgg
     6181 ggtggtcagt ggtgcgtcag ggggctgttc ggtaccgacc caaagcgcga ggccagtggg
     6241 gcaccggtgg cactcgaggt gggggttttc aatcgtgcga tcgaggtggc tctgctcata
     6301 aatctgtcgg catgcctcga cccggaggga gcacccgccg gggcaggtga cgaacaaaac
     6361 gccggggagc tgctcgggga atttttcagt cgcgcttacg cgttcaagtg ctgccgggtt
     6421 cggctgcgtc gattcgttcg tgtcgttatc gtcgttcact gctccccctg cgtcgattcg
     6481 ttcgtgtcgt tatcgtcgtt cactgctccc cctgcgtcga aatggtcgcc agacagcgcc
     6541 agcactggct gtgcgtcttt tttagtatct cacaaaaaac gccactttgg cgttaattgt
     6601 gttacgcttg tcttactgat tcctgacgag cagttgggaa acatgaaacc ggatcgcccg
     6661 gtgccgcttt ccctcctccc caacgaggcg gggttgtaaa cgaggatgcg cgcttcgtcc
     6721 ttggcaggtg cgagtaagcg agaagggctg ctgtcctgcc gctatgcggt aagtcctcaa
     6781 gggcggcttt atcgacaatc gtcactcctg cccccgatgc ccgttaagct gagggggcac
     6841 ctatctggta cacccaatga aaaaacgaat ctataccgct gacctcgccg cactattgaa
     6901 tgtgtcgctg cgtacgctct cgcggttaag ttcgcggggg atcgtgccga ctccggcagg
     6961 gcgctccctc cgtggcgggc attactggct ggctggcgat cccatgctca aacgctttat
     7021 tgccgaccag cgtcgtccgg ggagggctac tgatggcgtt cgataactct cacctggctg
     7081 acccgggtct attctggcgt gccgtgtcgg ctgcgggtgc tccggttgac cgtgaggact
     7141 ggctgcgcct ctgttccggc atagcgtgct gggcatctct ctgcggcgag gatgggctgg
     7201 cagctatcga ggcctggtct gcccgccaca atcgcgggga ggcaaaacac cggagccagt
     7261 accggcattt cctgaagctg aaaggcataa ccaatcccga ggctatggcg caggtgttcc
     7321 tgcagttctc cggcgtggag tggaaccgac aggcttctgt tcagtccgac gccgatcgcg
     7381 ctgaacggct gcggttgtcc cgtgaggcca cagcgaaggc aaaggctgaa cgcgacaaga
     7441 agggtgatgc tgctgcgcgt gctgcggtca agctgctggc gaagggaggg gatcctgcgt
     7501 ctaccccata ctggcgcaaa aagaccgctc tggcggctgc gggtggcgtc cagctgcccg
     7561 ccctcagcac agatcttggt gttggcggga tgtttcgatc gttcaatccg cgtgcttact
     7621 ggtctgattg cctcctgatt ccggtttaca agttcgacgc cgcccgtcag ctgtatgttg
     7681 acggcctgga ggccatctgc gggcgttgct cggctgaggg tttccagctc actggctcga
     7741 agttctctcc ggcgggtacc cgcaagcagg gcggcttcta cccgatgccg gggttcatgg
     7801 acgtgctcga cggctccccg cacgcgccct actcgcctct gttcgtctgt gaggggttcg
     7861 ctacggcgtt cgccctgcac cacctgagcc gtggcaaggt ggcggtctgc gctgcgttct
     7921 cgatcgccgg gttcggcagc tgcgtggcgt cgctgcgtcg gttctggcca gatgttgatg
     7981 tgatgctggt accggatcac ggcgaagcgt accgcctggc gcataaagcg gcctatggcg
     8041 atgagcgaga gccggtcccg acaattccag gcgcggcggt tgtgtcgttc gacggcctcg
     8101 atcggttcac tccccgggat aacctcgact ggttcgacgt gctggttgag gagggcgagg
     8161 atcgggcgcg tgagctgctc cggtatgcgg tgcgtcatgc ccggcttgag cggggctgat
     8221 ggctgggctt ttttgcgtcg gtttcctcgg gaggccgctg accgtaaagg gtcgttttct
     8281 ggctcgtctt ttttgcagtc gcttttgggt tggtgagaca acctttaaga cgcacatccg
     8341 accgcttccg gtttcctccg ttttagacgc cgctgcggtc gtgacgctat cctgcagctg
     8401 gtttggtttc cagcagcaca ctcaagcccg gcaccgtttt ggtgtcgggc tttttttcgc
     8461 ccgtaaggag ggcacatgaa atcaaagact gaattacgcg ctgccgcgac ggctcgcgcc
     8521 cttgaggtga tcgcttctga gatggggggt tggtcgctcg acgggtttgc tcacggctcg
     8581 ctgccgaact tctccccgct gccgcgccag cagacgcagg agggctctgt ggtgctcgaa
     8641 cgcccgccgt tcgactacac ctgggcgggt accgctgcgt ttaccgatcg tgcaaaccgt
     8701 gcgctgcagg tgaagctacc ggcgtcgcgt gagcgtaact acatctggct gtgcgcggtc
     8761 gaaagggagg ctgtggccac cgcgctgata gtggagtcgt tcaacgtgac cggctgcgcg
     8821 gcgttcgccg ggttgcctcc tgtggatggc atggtgctgc tgaccatgga cgaggccgac
     8881 gtggagctga tcagggcggc gatgctgccg tggctggatg cggcggcggc gtgagccgtc
     8941 gcccctctgc gtcaaatcag gccgcgctcg cgcagcttgt ggcggtagtc ccggacgcgg
     9001 ctggtattcc actcctgccc ggtggcggtg gtgtgcccgg ctgcgttcag cgcctgggcg
     9061 atcgcgggca gggtggcgtt ctccccggtc atgctgacga tgagctggtc ggcctcatcg
     9121 ctgacggcct ggaagcgggg atcggctccc cgggggacta tctgtcgtgc gttttgtggt
     9181 gcgctttgtc gtgctgtttg tggtgcagac tgtcgtgcat ctttcccgcc tccggccagc
     9241 gccctgacct ctgaccgcag cgcctcgatg gcggtcaaaa gtttggtgtc catcccttcc
     9301 ggcttcgtgc cgagtccggc gacaagcgcc tctatgacgg catctgtgcg ggttactttg
     9361 gttttctccg ctcgctgctc gatgcgctcg atcaggctga cgggcagtcg aacggttagg
     9421 gttctggttt tctctcgcat gggctggcct ctccgtgtcg tgcgttttgt cgtgctgttt
     9481 gtcttgcgga gtgtaatgcg ttttgtcgtg cgcgtcgaat ttgacgtagt gcaattgtcg
     9541 tgcagggagg ggtagcggct cggcccgtgg ataacttctg tggataacaa aatttcgaac
     9601 gttccggccc gtggataaat cgcggaggct gcgcggctct aagatcggtg cgggatcgcg
     9661 tttcgcgtgg ttggatcgtt gtgtactggt gcaggggggc atgtaacggt atgatggcgc
     9721 ggctgaaaaa gaaaacccgt ccgggcagac gggtcgtttt cttaaagctg ttttgagttt
     9781 ggagctttcg cggcggtcac cgtggaagac gccataataa ctcatgttgg gtgtgactcc
     9841 agcatgaatt atacgcgatc ataatgacgc gtgcaaaggg tgttttcccc gttatccccc
     9901 gccgttgtaa ggctcctcga gattggctgt tcaattttga ggatgtaaaa gtgtcaaatc
     9961 atgaagaaaa ttccggcact gctgccgaca acgtaacgcc gatcgccgct gccaaggtgg
    10021 gacgcccgtc tctacaaaaa aaatacccga ctaccgcgtt tcccgtggag ctggagatca
    10081 actaccggaa ggggcgctac tcgtcctctc ttgagaacgt tgtcgccgct atgcgctgcc
    10141 attccgtcac cggcatccag ctcgctttcg atgagttctt tgcggaggag ggggcgttcg
    10201 tgaacgggca atggtacccg ctgtccgaca acctagttac cgagctgcgt ctgcgcctcg
    10261 ctgctgccga ctttgagaag gtgaccaagg acaatgcccg cgacgccatg ttctacgtct
    10321 gcgatgagaa caaattcgac agcgcccagc gttggctgac ggctcaggaa tgggacggca
    10381 aacctcgcgt cagttacttc ttcgagcggt gcttcaaatg ccaaggggat cccgagtacc
    10441 tggcggcggt cagcctctac acctggacgg cgcttgccgg gcgcgtaatg aatcccggaa
    10501 tcaaggccga catggttccg ctgctgtacg ggcgacaggg tgcccgcaaa tcgacgggcg
    10561 ttcgccttat gtcgccattc cctgactcct actccagctt caacctgaac gacaaggacg
    10621 actccctagt gcgccgtatg cgtgggtgcc tgattggcga gctggaggag ctgcgcggcc
    10681 tccacgggcg cgactccgaa tcggtgaaag cgttcttctc cgctgcccac gacgagtggg
    10741 tgccgaaatt taaagagttc aaggtgcgaa acccgcgccg cgtggtgttc atcggtacca
    10801 cgaacaacaa gcagatcctc aatgactcga cggggaaccg ccgctatttg cccatgaatt
    10861 ccggggacgt ggactgcgcg gccatcgaga aaaaccgctc
//
