LOCUS       NZ_AP027763             2138 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017-02-1CC plasmid p2017-02-1CC_4, complete
            sequence.
ACCESSION   NZ_AP027763
VERSION     NZ_AP027763.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562622
            Assembly: GCF_036503635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 405x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036503635.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:12:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,955
            CDSs (total)                      :: 4,835
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 72 of 259
            Pseudo Genes (incomplete)         :: 175 of 259
            Pseudo Genes (internal stop)      :: 56 of 259
            Pseudo Genes (multiple problems)  :: 42 of 259
            Pseudo Genes (short protein)      :: 3 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027763.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017-02-1CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017-02-1CC_4"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            326..1321
                     /locus_tag="AABK15_RS24775"
                     /old_locus_tag="VEE73_47700"
     CDS             326..1321
                     /locus_tag="AABK15_RS24775"
                     /old_locus_tag="VEE73_47700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_038989441.1"
                     /translation="MQNPKLKNLTDYSPDDKPWDVHKSQSDDVGGIYLRAAEFEAYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDARWMFLTLTVRNCAIEDLGDTLTAMNAAFQRMKVRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPGMLNGHDYVKHYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPDWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDPD"
                     /name="protein rep"
     Typing          complement(1042..1232)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.375"
                     /coverage="98.0"
                     /evalue="1.4200000000000001e-44"
ORIGIN
        1 tgtatttata cttaagggat aactctagga ttcaaattcg cttccagccc ttgtgccgca
       61 aggcctgaga gccaatttgc tcgaaaatgg ttttgtgagt atgatttctg acaggtgatc
      121 gtgtccgcga actaattttt gattacttgg ggtggttccc aagaccttac ttactgacac
      181 gatgatatga gaaacccctg caagtgctac caacacttca ggggcgcgat cacctggcta
      241 aatacggttt gaaatcgact cgcaacaggt gatacggaaa tgataacaca cccttccagc
      301 gcctgcccat attggagcag gtgctatgca gaatcctaag ctaaagaacc ttactgacta
      361 cagtcccgac gataagccgt gggacgttca caagtcgcaa tctgatgatg ttggcggtat
      421 ctatctgcgt gcagctgaat ttgaggcgta tgcggctcgt atgcgcgatt gtggcggctt
      481 gctgcgtttt ggctggtcta cccttaaaga caccggagaa acgcgtttac ggcttcgtga
      541 ggcgcatttc tgccgcgttc gtcactgccc tgtctgccag tggaggcgtt ccctgatgtg
      601 gcaagcccgt ttttatcagt ctctgccgaa aatcgtcgct gattaccctg atgcccgctg
      661 gatgttcctc acgctcaccg ttcgcaactg tgcgatcgag gatctcgggg acacgcttac
      721 tgccatgaat gccgcattcc agcgcatgaa ggttcgcaag gaatttaaat ccgtacaggg
      781 atggattcgc accacagaag ttacccgcgg tcgtgatggc tctgcacacc cccactttca
      841 caccctgatg atggttcctc ctgggatgct gaacggtcat gactacgtta agcactaccg
      901 ctgggttgaa ctctggcgtg actgcctgcg cgttgattac gatcctaatg ttgatgttcg
      961 ggcagtgaaa ccccgcaagc ctaaagatgg ccagactctg gcatgtgcca ctgctgaact
     1021 ggtgcgcggt gccgttgctg aaaccctgaa atattcgaca aagccggctg atatggttgc
     1081 cgacccggac tggttcttag agctgacccg gcagacgcat aaacgccgtt ttgttgcgac
     1141 cggtggcgct ctgaaagacg ttctgaaact ggagcaggaa acggatcagg acatggtgat
     1201 cggggatgat atttccgatg gtgaagacga tggttcacgc ctcgcctttg gctgggattc
     1261 gacgccaaga aaatacgtcc gtttgcctga acgcgataag tttaaatcag atcctgatta
     1321 gggtgtcggg gcctgccctg accaggttgt ttttggatgc cgctgcgtgt ggcggtactc
     1381 caaaaacaca tcttgtccgg tacacagatt tcctttggca ttccttcggg tttccctgag
     1441 atttccactc tgcatacata cacgcataca cgaataatca acggcagaat tcgggcagga
     1501 tatgtaaagt gggaccactt tacgcctgcc ttattcgcac tctgctcaat ggaatattaa
     1561 atctgacagc gttcgccgtc gtcgaatcgt caacttctaa atcgggggat ccctttaggg
     1621 ttccgattta gtggttgtac gactgcaccc ctcataccct tgtgatgggt gatggttcac
     1681 atggtgagcc atcgccctgt aaagccgctt tttaccccct tcaaggcgtt gcctgtgggg
     1741 taggtgcgta agccctgccc tacaggtgat gccgtggggg tattttcgtt ttgctcggta
     1801 tgccttattc gtttaaatcc gccatcagga gtatgcgggg taaagtcgtg cggccgcgat
     1861 gaggaacggg gattgccccc gcagctctgc gaggaggcac acgccgttcg gaatcggggt
     1921 ccactctgtt taatagtgga ctcttgttgc gatttgcaac ggaaacagcc gcgtgcagtg
     1981 aatgtaatac tgcacgtttg tctcaatctg acatttctca ggggggggcg atttaacgtg
     2041 gtttgcttaa tttttgagct tacgaatgct tgagtgtttg agtgcctgaa tgctgctttt
     2101 gggggttggt ttttcggccc tgaaaggggc gaaatcct
//
