LOCUS       NZ_AP027762             2442 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017-02-1CC plasmid p2017-02-1CC_3, complete
            sequence.
ACCESSION   NZ_AP027762
VERSION     NZ_AP027762.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562622
            Assembly: GCF_036503635.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 405x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036503635.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:12:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,955
            CDSs (total)                      :: 4,835
            Genes (coding)                    :: 4,576
            CDSs (with protein)               :: 4,576
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 72 of 259
            Pseudo Genes (incomplete)         :: 175 of 259
            Pseudo Genes (internal stop)      :: 56 of 259
            Pseudo Genes (multiple problems)  :: 42 of 259
            Pseudo Genes (short protein)      :: 3 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027762.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017-02-1CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017-02-1CC_3"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            join(1627..2442,1..153)
                     /gene="mobV"
                     /locus_tag="AABK15_RS24760"
     CDS             join(1627..2442,1..153)
                     /gene="mobV"
                     /locus_tag="AABK15_RS24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(223..453)
                     /locus_tag="AABK15_RS24765"
                     /old_locus_tag="VEE73_47680"
     CDS             complement(223..453)
                     /locus_tag="AABK15_RS24765"
                     /old_locus_tag="VEE73_47680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(440..880)
                     /locus_tag="AABK15_RS24770"
                     /old_locus_tag="VEE73_47690"
     CDS             complement(440..880)
                     /locus_tag="AABK15_RS24770"
                     /old_locus_tag="VEE73_47690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000344119.1"
                     /translation="MDENGEVKQDSKIETFSIPAEPPYVKLYIADIAMIHGLSKSQNDV
                     LLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVDKQIFFRAGRGELEA
                     NPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          1619..2442
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.545"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 tttaaggggc tttccagaga gcagatgacc gaaatcctga tgatggctca gcggtttaag
       61 cagcaaaacc aggaaaagga aaaacagcag cgtattgaac gtgaaaaaca gcgacagacg
      121 cgatcgcgtg gcatgggtgg tatggagcgt tagttttgat gcgatgcagg atagggctaa
      181 cgccgcaaag tgacgtcatt tgcggcattc accacgaaaa aattaatcat tcagatggta
      241 attatgtttt tttaaaatag aaaaagctgc gtttttctgc tttggtgaca aacccttagt
      301 tgagtattta ttaccgctag ttctacgata tatgctgtaa acactgctta aaaaagtacg
      361 ctcccaaaca gtcagaacct cattcttttt tagtgaatca tgcagcatta gcctgtctac
      421 atttttaaaa agtttatctt cagcattcat catccattac cttcgtctgc accgttcttc
      481 cattggtgtc ataactgatc tttaactcaa ttttctttct tctttcgtaa attgatggcc
      541 agtctccccg tgcaaaaaga aaggggttag cttcaagttc cccacgccct gcccggaaga
      601 atatttgttt atctacaaga gccgcaatta tgtttttaaa ggtctgatat tcaatatgaa
      661 ccctgggctg tatggttttt tcaaatctag tttttgatac agacacaaac ccatcccatc
      721 caatcagcat tgccaattgt aaaagcacat cattttgaga ttttgataaa ccatgaatca
      781 tcgcaatgtc tgcaatgtat agcttgacgt agggaggttc ggctgggatg gaaaaggtct
      841 cgatcttaga gtcttgtttt acctcaccgt tttcatccat gtatgtatgc cgttcaagcc
      901 tagaaatctt tttcgtattc actccagccc cacgtttcgc catgatttag tactcgcatt
      961 taagtactaa agagacatac agtcaagtcc tttgtatcaa gtacgattag tacttgaggt
     1021 gagtacgatt agtccttgag gtgagtacga ttagtacttg aggtgagtac gattagtact
     1081 tgaggtgagt acgattcttc gctctagccc ttggtacaag cgggttccca tatctatatc
     1141 tatatattaa gggggaaaat aagggctgaa aaacggcatt tttagacact gaaaaactgc
     1201 ctttttttag caaggagtgt acgcatttct atgtacgggg agtggaaatt ccgcttcctc
     1261 gctttggctg ttcgttcctc gtcgcgttgc tcctgcggta cgcctcagcc aaagctgcgg
     1321 cgagcggttc ctgctctttc aaagacgatt tcattgcctg atttcgtcaa gggcgagcgt
     1381 agcgagtgcc atttaccctt gacgacactc cggataaaac aggcttggca gtggtggggg
     1441 gtggcgcgaa gcgacgcctt ccacctgcca gaccgccgtt gattttgacc ttctggaagc
     1501 ttgctgcccg aacgattttg cgtagcaaag tctagtgagt acactatcat cttgatagtg
     1561 gcccgccgga ggtatcgcaa tgcgctcccc cgcctggctg aatgaatgcc ggaatcaggg
     1621 gggaacatgg cgaactacgc gataatgcgc tgcaaaaagc tgacgggaat gggaagcgtg
     1681 gccagcgcgt tgcagcactg ctaccgagag cgtgagacgc cgaacgctaa cgctgaacgg
     1741 acaccagaaa actactgctc tgtgtccaaa tcaacggatc aggcaatggg gcgtgtgcgg
     1801 gagctgctgc cggaaaaacg ccgtaaagat gcggtactgg ccgttgaata cgttatgaca
     1861 gcaagccctg agtggtggaa agaggccaca ccacggcagc aggcggagtt tttcgcccgc
     1921 tctgagcaat ggctggagaa gaaatacggg aaggatcgtg ttgtggcggc ggtggttcac
     1981 cgggacgagg ccaccccgca cctgtctgcg ttcgtggtgc cattgacgca ggatggacgg
     2041 ctgtccgcga aagagtttat tggcgggcgg tcaaagatgc gtgaagatca gagcacctac
     2101 gctgaatcag taaaaaaact gggtcttgag cgcggcatcg agggaagccg cgccacgcat
     2161 cagacagttc agcactacta cgaatcgata aaccgtggca cccgtagcca ggtatcgatc
     2221 tcgccggagg cgctggagcc gcgtgtattg cggaagggca ttttcaccaa agacgtggaa
     2281 gatcaggcag ccatagccaa acgtctaagc cacgcagtga acgatggctt tgcaggaacc
     2341 atcgctatgg cctctcagag cgcccagaac gcaaaaagag cgcgtgagct acagaagact
     2401 atggatgcgc agcagaaacg cctacagagc gtcacagagc ct
//
