LOCUS       NZ_AP027751             8818 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017.02.03CC plasmid p2017.02.03CC_3,
            complete sequence.
ACCESSION   NZ_AP027751
VERSION     NZ_AP027751.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562586
            Assembly: GCF_036503275.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 236x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036503275.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:07:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,751
            CDSs (total)                      :: 4,636
            Genes (coding)                    :: 4,406
            CDSs (with protein)               :: 4,406
            Genes (RNA)                       :: 115
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 141 of 230
            Pseudo Genes (internal stop)      :: 40 of 230
            Pseudo Genes (multiple problems)  :: 43 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027751.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017.02.03CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017.02.03CC_3"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            58..918
                     /locus_tag="AABK06_RS23680"
                     /old_locus_tag="VEE50_45390"
     CDS             58..918
                     /locus_tag="AABK06_RS23680"
                     /old_locus_tag="VEE50_45390"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-135"
                     /protein_id="WP_015058868.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTTPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-135"
     gene            1293..1853
                     /locus_tag="AABK06_RS23685"
                     /old_locus_tag="VEE50_45400"
     CDS             1293..1853
                     /locus_tag="AABK06_RS23685"
                     /old_locus_tag="VEE50_45400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            1857..>2228
                     /locus_tag="AABK06_RS23690"
                     /pseudo=""
     CDS             1857..>2228
                     /locus_tag="AABK06_RS23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            2208..2453
                     /locus_tag="AABK06_RS23695"
     CDS             2208..2453
                     /locus_tag="AABK06_RS23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041937891.1"
                     /translation="MSDFRALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(2485..3135)
                     /gene="tetR(A)"
                     /locus_tag="AABK06_RS23700"
                     /old_locus_tag="VEE50_45420"
     CDS             complement(2485..3135)
                     /gene="tetR(A)"
                     /locus_tag="AABK06_RS23700"
                     /old_locus_tag="VEE50_45420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_038989485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEARPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            3241..4440
                     /gene="tet(A)"
                     /locus_tag="AABK06_RS23705"
                     /old_locus_tag="VEE50_45430"
     ARG             3242..4437
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(4472..5356)
                     /locus_tag="AABK06_RS23710"
                     /old_locus_tag="VEE50_45440"
     CDS             complement(4472..5356)
                     /locus_tag="AABK06_RS23710"
                     /old_locus_tag="VEE50_45440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(5494..5886)
                     /locus_tag="AABK06_RS23715"
     CDS             complement(5494..5886)
                     /locus_tag="AABK06_RS23715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <5890..6754
                     /locus_tag="AABK06_RS23720"
                     /pseudo=""
     CDS             <5890..6754
                     /locus_tag="AABK06_RS23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            7685..7876
                     /locus_tag="AABK06_RS23725"
                     /old_locus_tag="VEE50_45480"
     CDS             7685..7876
                     /locus_tag="AABK06_RS23725"
                     /old_locus_tag="VEE50_45480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8066..8763)
                     /locus_tag="AABK06_RS23730"
     CDS             complement(join(8066..8514,8514..8763))
                     /locus_tag="AABK06_RS23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     ARG             59..915
                     /name="blaTEM-135"
                     /gene="blaTEM-135"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-135"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7191..7810)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.243"
                     /coverage="99.0"
                     /evalue="1.1799999999999999e-174"
ORIGIN
        1 ccgctcatga gacaataacc ctggtaaatg cttcaataat attgaaaaag gaagagtatg
       61 agtattcaac attttcgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      121 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      181 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      241 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggtgcggt attatcccgt
      301 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      361 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      421 agtgctgcca taaccatgag tgataacact gctgccaact tacttctgac aacgatcgga
      481 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      541 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgacgcct
      601 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      661 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      721 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      781 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      841 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      901 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      961 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccggaaa cgataggtgc
     1081 aagcaagtta agggttgcat cgcgcatgtc aatctaggct ataccctaac ttgatgtcag
     1141 gcagggccgc gccgcttcgt cagaatagag tctgctttcc cattttttga cacatgcccg
     1201 cgaaggttat agatttcagc ctgacagaaa tgggctttga ggcacaacgg aacagaaagt
     1261 gcacttaagc cgccttcaac caaggagaca tcgtgcaggg gcaccgcatc ggctacgtcc
     1321 gggtcagcag ctttgaccag aacccggaac gccagctgga acaaacccag gtgagcaagg
     1381 tgttcaccga caaggcatcg ggcaaggaca cccagcgccc ccagctcgaa gcgctgctga
     1441 gcttcgtccg cgaaggcgat acagtggtgg tgcacagcat ggatcggctg gcccgcaacc
     1501 tcgatgacct gcgtcgcttg gtacagaagc tgactcagcg cggcgtgcgc atcgagttcc
     1561 tgaaggaggg cctggtgttc actggcgagg actcgccgat ggccaacctg atgctgtcgg
     1621 tgatgggggc cttcgctgag ttcgagcgcg ccctgatccg cgagcggcag cgtgagggca
     1681 tcgccttggc caagcagcgt ggcgcgtacc ggggccgcaa gaaagccctg tccgatgagc
     1741 aggctgctac cctgcggcag cgagcgacgg ccggcgagcc caaggcgcag cttgcccgcg
     1801 agttcaacat cagccgggaa accctctacc agtacctccg cacggacgac tgacacatgc
     1861 cgcgtcgctt gatcctctcg gccacggagc gggacaccct gcttgcgctg ccggaaagcc
     1921 aggatgacct gatccgctac tacaccttca acgactccga cctgtcgctg atccgccagc
     1981 gacgcggcga cgccaaccgc ctcggcttcg ccgtgcagct ctgcctgctg cgctaccccg
     2041 gttacgcgct gggaaccgac agcgagctgc ccgagccggt catcctgtgg gtggcgaagc
     2101 aagtccaggc cgagccggcg agctgggcaa agtacggcga gcgcgacgtg acccgtcgcg
     2161 agcatgccca ggaactgcgc acctacctgc aactggcccc gttcggcctg tccgacttcc
     2221 gcgccctgga cctcgcccgc gagcgccagg cgcacgaggc cggcgcgcgg acccgcgcca
     2281 cggcccacga gcggacgccg cagcaggagc gccagaaggc cgccagagag gccgagcgcg
     2341 gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc
     2401 gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg
     2461 gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct
     2521 gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc
     2581 tgcgtccgga cgggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg
     2641 agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag
     2701 cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga
     2761 aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc
     2821 cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa
     2881 gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg
     2941 cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct
     3001 gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt
     3061 cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg
     3121 ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta
     3181 tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac
     3241 gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc
     3301 atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac
     3361 gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt tgcctgcgca
     3421 cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct cgtctcgctg
     3481 gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg ggttctctat
     3541 atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt
     3601 gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc
     3661 gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcgattt ctccccccac
     3721 gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt
     3781 ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc
     3841 gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc
     3901 atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc
     3961 tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca
     4021 ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc
     4081 atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga
     4141 tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg
     4201 caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg
     4261 gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg
     4321 gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc
     4381 tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga
     4441 tcgtggaaac gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg
     4501 ccctaggagt gcggttggaa cgttggccca gccagatact cccgatcacg agcaggacgc
     4561 cgatgatttg aagcgcactc agcgtctgat ccaagaacaa ccatcctagc aacacggcgg
     4621 tccccgggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
     4681 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
     4741 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
     4801 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
     4861 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
     4921 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
     4981 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
     5041 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
     5101 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
     5161 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
     5221 ccgccggcaa cgcccgcagc atcgcgaccg tcatcggtga gaagttcggc aggtattggg
     5281 tggtgacaat gtaggtgctg ccccaaatgg caggtgctat cgctgtgaac aataaatcgg
     5341 gcgtgcgtaa tgacatgcga ttacaagacc tccgctacgg cgatgggttt ttgagagttg
     5401 caggcgccgc gatgaccgca ccctgacttg gactggccct gcctcgcacc aatccacctg
     5461 cggctatggc cgcgatcatg cgaggcccga aaattacacg acctgtacac gtccgccgaa
     5521 cccggaacgc aacagagctt cgtgggcatc ccgatccggg tcgtagcagc agtcctggac
     5581 caaacgcacg tcgtagtccg catcactagc ccaggcgacg cttgaaagaa caacgccggt
     5641 ggtgcttatc ccggccatga caagcgtgct tacgccgcgc gtccgaaggt cggcgtcgag
     5701 cgccgtgcca taaaagacgc tggctcgcgg acatgcatag aaaaggtcgc cccgttcgat
     5761 cgcaagccct tcgacgggca gaccggtgcg aaatcgtccg ctcgggagat acggtgagat
     5821 ttggcgattc gtcgccggcg gcgcatgttc atactcttcg ccgagcgaaa agttggggaa
     5881 cagcacgggc cggccgcgca aggcgtgctg gcagccgtgc agaccctgcg tgagatgaac
     5941 gccgacaacc tgcgcaaggt gccggccgat gcacccacgg ccttcatcaa gccgcgctgg
     6001 aagccgctgg tgatcacccc ggaaggcctc gaccggaaat tctacgaaat ctgcgccctg
     6061 tccgagctga agaacgccct gcgctccggc gacatctggg tcaagggctc gcggcagttc
     6121 cgcgacttcg acgactacct gctgccggcc gagaagttcg ccgcactcaa gcgcgagcag
     6181 gccctgcccc tggcgatcaa cccgaacagc gaccagtacc tggaagagcg tttgcagctg
     6241 ctggacgagc agttggccac cgtcacccgc ctggccaagg acaacgagct gcccgatgcc
     6301 atcctcaccg agtcagggct gaaaatcacc ccgctggatg cggcggtgcc ggatcgagcg
     6361 cacgctgttc atcctggact ggctgcaaag tgttgaactg cgccgccgcg tgcatgccgg
     6421 cctgaacaaa ggtgaggcgc gcaactcgct ggccagggcg gtgttcttca accgccttgg
     6481 ggaaatcagg gatcggagct tcgagcagca gcgctaccgg gccagcggcc tcaacctggt
     6541 gacggcggct atcgtgctgt ggaacacggt gtacctggaa cgcgccaccc aggggttggt
     6601 cgaggccggc aagccggtgg acggcgagct gctgcaattc ctgtcgccgc tgggctggga
     6661 gcacatcaac ctaaccggcg attacgtctg gcggcagagc cgcagactgg aagacgggaa
     6721 gtttcggccc ttacggatgc ccggaaaacc ttagcgtacg attttttccg aattctgcgg
     6781 gctcccctat gtgggcgttt atgtaacata tgtcgctggg ccaactcttc tggtttctgg
     6841 gtttttatcg tggattacaa ggccgtaatc tgataatgcc cctaatgggt cagatctcta
     6901 ggatagtagc aatacagcaa ggaacttctt gagatccttt ctgttgcgtc gtaatctcat
     6961 gtcctgcaaa caaaaaaacc accgctacca acggtggttc gtttgccgga atcaagagct
     7021 accaactctt ttttccgaag gtaactggct ttcacggagc gcagatacca aaactgtcct
     7081 tctagtgtag ccgcagtcag gccaccactt caagaactct gtagaagcac tctactactc
     7141 tgctccgtga atcctgttac cagtggctgc tgccagtggc gcatttgcat gtctttccgg
     7201 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     7261 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     7321 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     7381 agcgcacgag ggagccacca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7441 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     7501 aaaacgcccc gctggggcgt ggccttttcg cccttgtcct tagctttcgt tatgcttacc
     7561 gttacgttat ctgcttatct tccctccgct cgccgcagac tcatgaccga acggagtgag
     7621 cgaatgagcg aggaagcgga aaaccatcag aacgtacagt aagcactttc gcgccggggc
     7681 cttcatgaac aaacaacaaa agacagccgt taacatggct aaattcatcc aggatcagtc
     7741 cctgcttctt ctggacaggc ttaatgagct ggatctggat catgaggctg atttctgcga
     7801 gaaactccat gaggatgcgg aacgccttta ccgaagcctg tcagcgcgcc tgaatgagca
     7861 gcaggacggt gcgtaatggc tccgttgtgg gtattaccgg agtaataccg gcatcgcctt
     7921 aattttcatt gtcttggtgc ttaatattat taccccggta atacccagaa tccctttatt
     7981 ttttcttttt tatttatttt tcatgaagtt gcgatgaaaa tcgcagctgc gttaggtgta
     8041 tgtcattttt ggtaatgact ccaacttact gatagtgttt tatgttcaga taatgcccga
     8101 tgactttgtc atgcagctcc accgattttg agaacgacag cgacttccgt cccagccgtg
     8161 ccaggtgctg cctcagattc aggttatgcc gctcaattcg ctgcgtatat cgcttgctga
     8221 ttacgtgcag ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatccata
     8281 tcaccacgtc aaagggtgac agcaggctca taagacgccc cagcgtcgcc atagtgcgtt
     8341 caccgaatac gtgcgcaaca accgtcttcc ggagcctgtc atacgcgtaa aacagccagc
     8401 gctggcgcga tttagccccg acgtatcccc actgttcgtc catttccgcg cagacgatga
     8461 cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt ttaaatggcg
     8521 gaaaatcgtg ttgaggccaa cgcccataat gcgggcggtt gcccggcatc caacgccatt
     8581 catggccata tcaatgattt tctggtgcgt accgggttga gaagcggtgt aagtgaactg
     8641 cagttgccat gttttacggc agtgagagca gagatagcgc tgatgtccgg cagtgctttt
     8701 gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc
     8761 cactggagca cctcaaaaac accatcatac actaaatcag taagttggca tcatcacc
//
