LOCUS       NZ_AP027747             2164 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain 2017-01-4CC plasmid p2017-01-4CC_2, complete
            sequence.
ACCESSION   NZ_AP027747
VERSION     NZ_AP027747.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562619
            Assembly: GCF_036503025.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 598x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036503025.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:01:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,567
            Genes (coding)                    :: 4,321
            CDSs (with protein)               :: 4,321
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 246
            CDSs (without protein)            :: 246
            Pseudo Genes (ambiguous residues) :: 0 of 246
            Pseudo Genes (frameshifted)       :: 68 of 246
            Pseudo Genes (incomplete)         :: 169 of 246
            Pseudo Genes (internal stop)      :: 41 of 246
            Pseudo Genes (multiple problems)  :: 32 of 246
            Pseudo Genes (short protein)      :: 5 of 246
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027747.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2017-01-4CC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2017-01-4CC_2"
                     /geo_loc_name="Viet Nam:Thai Binh"
                     /collection_date="2017"
     gene            complement(280..708)
                     /locus_tag="AABJ81_RS23435"
                     /old_locus_tag="VEE70_44800"
     CDS             complement(280..708)
                     /locus_tag="AABJ81_RS23435"
                     /old_locus_tag="VEE70_44800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_130559423.1"
                     /translation="MSLNMTFSITEVREGRIGSLVITKELYDKLEKYSRSNGLITSTYD
                     HGHGVVRYYTVKYCLQMNYHGQNHPQKYLIKRGGRYPSKYAMVKKTVEELEYSTPTFDA
                     ICRNIAIKYPHMVKFANYISETPLKGFAATFNNSVGND"
                     /name="hypothetical protein"
     gene            1474..1665
                     /locus_tag="AABJ81_RS23440"
     CDS             1474..1665
                     /locus_tag="AABJ81_RS23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(943..1295)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.465"
                     /coverage="86.0"
                     /evalue="2.13e-170"
ORIGIN
        1 cggctgcgcc ggcaacgcct cacggccacg caataaattg cgtccccgtt caccgttttc
       61 gggtaagaag aacatcggaa acaggactta ccccggctga atggcgtgaa tttccgcgct
      121 atgcacttgt gcgcatactc atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg
      181 ttttcgggtg gtttgttgcc tgttttgccg gtttcccgtc agaaacgctc tgagggcgcc
      241 tgaggcggtg cgggcaaggg atgttatggt aaataacact taatcattac caacggagtt
      301 attaaaggtt gcggcgaagc cttttaatgg ggtttctgat atgtaattag caaacttgac
      361 catgtgtggg tattttattg ctatgttcct gcatattgcg tcaaatgtcg gtgttgaata
      421 ctctaactct tcaacggttt ttttgaccat tgcatacttg ctgggatatc tcccccctct
      481 tttaataaga tatttttgag gatgattttg tccgtgatag ttcatctgga ggcaatattt
      541 cacggtgtag tatctaacaa cgccatgacc atggtcgtat gtggatgtta ttaatccatt
      601 tgagcgagag tatttttcta atttatcgta aagctctttt gtaataacta aagagccgat
      661 acgcccctca cgcacttctg ttattgaaaa agtcatattt aagctcactt ttttactctg
      721 aatataacct gttagagagg attttcttga gattatccgt ctgtcgtcgt agtctcttat
      781 ccagaaacga aaaaaccgcc ctgcaaggcg gtttttcgaa ggttctcaga gctaccaact
      841 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
      901 agccttaacc ggcacatgac ttcaagtcca tctcctctaa atcagttacc agtggctgct
      961 gccagtggcg cttttgcatg tcttaccggg ttggactcaa gacgatagtt accggataag
     1021 gcgcagcagt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     1081 ttcccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     1141 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag ggggaaacgc
     1201 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     1261 atgcttgtca ggggggcgga gcctatggaa aaacgccccg gcggggcgtg gccttttcgc
     1321 ccttgtcctt agctttcgtt atgcttaccg ttatgttatc tgcttatctt ccctccgctc
     1381 gccgcagact catgaccgaa cggagtgagc gaatgagcga ggaagcggaa aaccatcaga
     1441 acgtacagta agcactttcg cgccggggcc ttcatgaaca aacaacaaaa gactgccgtt
     1501 aacatggcta aattcatcca ggatcagtcc ctgcttcttc tggacaggct taatgagctg
     1561 gatctggatc atgaggctga tttctgcgag aaactccatg aggatgcgga acgcctttac
     1621 cgaagcctgt cagcgcgcct gaatgagcag caggacggtg cgtaatggct ccgttctggg
     1681 tattaccgga gtaataccgg catcgcctta attttcattg tcttggcgct taacattatt
     1741 accccggtaa tacccagaat ccctgtattt tttctttttt atttattttt catgaagttg
     1801 cgatgaaaat cgcggctgcg ttaggtgtat gaccatttaa gggttaaaaa atcatcattt
     1861 ttggtagggc gctctccgta ggttaagggt tatttggcta taaagcgtcc tattctttga
     1921 tggtcatgct tgcatgacca tctgagcaac caaaaactac agataaacta caaaaaacgt
     1981 tttgcattag tgatggcaga ctacaaaaaa actacaagaa aactacaaaa aacgtggcag
     2041 actactaata gactacaaga aaactacaga taaactacaa agtaactacc ggattacctc
     2101 agatccgttc cctgaaccat tcgtacatcg acccccttcc tgaacgtgct gaaaacgggt
     2161 tttc
//
