LOCUS       NZ_AP027701             6768 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSS-12 plasmid pPSS-12_4, complete sequence.
ACCESSION   NZ_AP027701
VERSION     NZ_AP027701.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562525
            Assembly: GCF_036501905.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 241x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036501905.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:11:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,771
            CDSs (total)                      :: 4,656
            Genes (coding)                    :: 4,432
            CDSs (with protein)               :: 4,432
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 224
            CDSs (without protein)            :: 224
            Pseudo Genes (ambiguous residues) :: 0 of 224
            Pseudo Genes (frameshifted)       :: 64 of 224
            Pseudo Genes (incomplete)         :: 147 of 224
            Pseudo Genes (internal stop)      :: 46 of 224
            Pseudo Genes (multiple problems)  :: 34 of 224
            Pseudo Genes (short protein)      :: 4 of 224
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027701.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSS-12"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSS-12_4"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            join(6386..6768,1..58)
                     /locus_tag="AABK13_RS23755"
     CDS             join(6386..6768,1..58)
                     /locus_tag="AABK13_RS23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(803..952)
                     /locus_tag="AABK13_RS23760"
                     /old_locus_tag="VEE19_46130"
     CDS             complement(803..952)
                     /locus_tag="AABK13_RS23760"
                     /old_locus_tag="VEE19_46130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1523..1681
                     /locus_tag="AABK13_RS23765"
     CDS             1523..1681
                     /locus_tag="AABK13_RS23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2141..2956
                     /gene="sul2"
                     /locus_tag="AABK13_RS23770"
                     /old_locus_tag="VEE19_46140"
     ARG             2142..2953
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3017..>3544
                     /locus_tag="AABK13_RS23775"
                     /pseudo=""
     CDS             3017..>3544
                     /locus_tag="AABK13_RS23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /name="phosphotransferase"
     gene            3565..4038
                     /gene="dfrA14"
                     /locus_tag="AABK13_RS23780"
                     /old_locus_tag="VEE19_46160"
     ARG             3566..4035
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            <4113..4388
                     /locus_tag="AABK13_RS23785"
                     /old_locus_tag="VEE19_46170"
                     /pseudo=""
     CDS             <4113..4388
                     /locus_tag="AABK13_RS23785"
                     /old_locus_tag="VEE19_46170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /name="aminoglycoside phosphotransferase APH(3')"
     gene            4388..5224
                     /locus_tag="AABK13_RS23790"
                     /old_locus_tag="VEE19_46180"
     CDS             4388..5224
                     /locus_tag="AABK13_RS23790"
                     /old_locus_tag="VEE19_46180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<5331..5735)
                     /locus_tag="AABK13_RS23795"
                     /pseudo=""
     CDS             complement(<5331..5735)
                     /locus_tag="AABK13_RS23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5779..6168)
                     /locus_tag="AABK13_RS23800"
                     /old_locus_tag="VEE19_46190"
     CDS             complement(5779..6168)
                     /locus_tag="AABK13_RS23800"
                     /old_locus_tag="VEE19_46190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             4389..5221
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4144..4388
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="30.34"
     ARG             3018..3559
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.43"
                     /coverage="67.42"
     Typing          complement(988..1161)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1085..1690
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggcacaggaa aaaaagcggg gattctggtc gcggctgttt cgctccggtt cagagtaaca
       61 gtgcaggtga ctctgattta ccataacatc tgttatcagc accgcctgag aggccctcag
      121 ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa
      181 cgcgcaggct ctgtttttac ggcatgcatg agtatgcgca caagtgcata gcgcggaaat
      241 tcacaccatt cagccggggt aagtcctgtt tccggcgttc ttcccatccg aaaacggtga
      301 acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaacccgct
      361 gtcagcacgt tcaggaaggg ggtgatgtac aaatggcaca gaggatgaaa gtgacgtatg
      421 agctgaagga atgatgtaaa agtgatgtat aagtgatgta aaagtgatgt atgaactgaa
      481 ggagcgatgt aattctgtag tcaggaagaa taacccgttc atgcaggcat gaacgataca
      541 gtgaggaacg ctttttttgt cacgatctcc ccttaacctg cgtaaatcac ccgccatggt
      601 tgattatttt tcaacctcaa aatggtcata cacctaacgc agccgcgatt ttcatcgcaa
      661 ctgcatgaat taaaagaaaa aaaaggaata tttcgttttc acgagtaata cccgggtatt
      721 aaaaatccac tgtaattaca tgaaaataaa gaaataaaag gtaataccgg agtattacca
      781 cgtacgcggg caagcgaaaa agctataacg cttcccggcc gacaggcgac ccaaaatcca
      841 caggggcatg caggttctct tcagtaacct gcgccattaa atcaaaaagg tcatattcgc
      901 cgggaagctg cgtatcagtc gaatccagtt gttcatcata ttcagatggc atggggtgat
      961 atccagtagc tgagaggttg ttagcgggtt tcggggcgca gccctgaacc agtcatgtag
     1021 cgctagcgga gtgtatactg gcttactatg ttgccacgat tgcgaaaatc agtgaagtgc
     1081 ttcatgtggc aggagaaaaa aggcagcacc ggcgcgtcag cagaatatgc gatacaggag
     1141 atattccgct tcctcgctca ctgactcgct acgctcggtc gttcggctgc ggcgagcggt
     1201 accggcttac ggacggggac ggaaatttcc tggaagatac caggaagaca cttagcaaag
     1261 aagagataag gccacggcga agccgttttt ccataggctc cgcccccctg acaagcatcg
     1321 caaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatcccaggc
     1381 gtttccccct ggtagctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1441 cattccgctg ttatggccgc ggtttctcat tccacgcctg acactcagtt ccgtgaaggc
     1501 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
     1561 gtccggtaac tatcaacttg agtccaaccc ggaaagacac gacaaaacgc cactggcagc
     1621 agccattagt aacaggagtg cacgagaaga tagattaaga ggtcttgaag tagaggcctg
     1681 actgcggcta cactgaaaga acagttttgg taatggcggt ctcgtgaacc ctgttaccgg
     1741 gttaagcagt tccccaactg acttaacctt cgaacaaacc gcctccccag gcggtttttt
     1801 cgtttacaga gcaagagatt acgattcatt gaaaaggatc tcaagaagat cctttgtaga
     1861 ttactatcaa ttaatcttcc gaacatgaca ccagtcactg ttgattacgg ttaaaccgct
     1921 ccgtaacagg tttccctcgt attggtgact tgcagcacca aagaagcaaa aaagataaat
     1981 atatctactt attgagagat gatatattta tttccaaaat gctacagata catcatcatg
     2041 aaacggatca ccgcaaacag gttactcgcc tggggattcc ctttcgaccc gagcatccgt
     2101 atgagactca tgctcgatta ttattattat agaagccccc atgaataaat cgctcatcat
     2161 tttcggcatc gtcaacataa cctcggacag tttctccgat ggaggccggt atctggcgcc
     2221 agacgcagcc attgcgcagg cgcgtaagct gatggccgag ggggcagatg tgatcgacct
     2281 cggtccggca tccagcaatc ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg
     2341 tatcgcgccg gtgctggacg cgctcaaggc agatggcatt cccgtctcgc tcgacagtta
     2401 tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg gcctatctca atgatattcg
     2461 cggttttcca gacgctgcgt tctatccgca attggcgaaa tcatctgcca aactcgtcgt
     2521 tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat
     2581 ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa
     2641 acgcaaccgc cttgtccttg atcccggcat ggggtttttt ctgggggctg ctcccgaaac
     2701 ctcgctctcg gtgctggcgc ggttcgatga attgcggctg cgcttcgatt tgccggtgct
     2761 tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt
     2821 cggggccgcg acactcgctg cagagcttgc cgccgccgca ggtggagctg acttcatccg
     2881 cacacacgag ccgcgcccct tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac
     2941 cgcaagaatt cgttaactgc acattcggga tatttctcta tattcgcgct tcatcagaaa
     3001 actgaaggaa cctccattga atcgaactaa tatttttttt ggtgaatcgc attctgactg
     3061 gttgcctgtc agaggcggag aatctggtga ttttgttttt cgacgtggtg acgggcatgc
     3121 cttcgcgaaa atcgcacctg cttcccgccg cggtgagctc gctggagagc gtgaccgcct
     3181 catttggctc aaaggtcgag gtgtggcttg ccccgaggtc atcaactggc aggaggaaca
     3241 ggagggtgca tgcttggtga taacggcaat tccgggagta ccggcggctg atctgtctgg
     3301 agcggatttg ctcaaagcgt ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct
     3361 atcggttgat caatgtccgt ttgagcgcag gctgtcgcga atgttcggac gcgccgttga
     3421 tgtggtgtcc cgcaatgccg tcaatcccga cttcttaccg gacgaggaca agagtacgcc
     3481 gcagctcgat cttttggctc gtgtcgaacg agagctaccg gtgcggctcg accaagagcg
     3541 caccgttaac ccaggatgag aaccttgaaa gtatcattga tagctgcgaa agcgaaaaac
     3601 ggcgtgattg gttgcggtcc agacataccc tggtccgcga aaggggagca gctacttttt
     3661 aaagcattga cctacaatca gtggcttctg gtgggtcgca agacgtttga atctatgggc
     3721 gcactcccca ataggaaata cgcggtcgtt acccgctcag gttggacatc aaatgatgac
     3781 aatgtagttg tatttcagtc aatcgaagag gccatggaca ggctagctga attcaccggt
     3841 cacgttatag tgtctggtgg cggagaaatt taccgagaaa cattacccat ggcctctacg
     3901 ctccacttat cgacgatcga catcgagcca gagggggatg ttttcttccc gagtattcca
     3961 aataccttcg aagttgtttt tgagcaacac tttacttcaa acattaacta ttgctatcaa
     4021 atttggaaaa agggttaaca aagctatgca atcgacggca aaaagcttcg ttcgcttcgc
     4081 gcactacgcc tttttccgcg attgatagcg acgatatggt tgtttgccat ggtgatccct
     4141 gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg
     4201 ggcggctcgg aacagcagat cgctatgccg atttggcact catgattgct aacgccgaag
     4261 agaactgggc agcgccagat gaagcagagc gcgccttcgc tgtcctattc aatgtattgg
     4321 ggatcgaagc ccccgaccgc gaacgccttg ccttctatct gcgattggac cctctgactt
     4381 ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc aacctgttct
     4441 cattgcggac accttttcca gcctcgtttg gaaagtttca ttgccagacg ggactcctgc
     4501 aatcgtcaag ggattgaaac ctatagaaga cattgctgat gaactgcgcg gggccgacta
     4561 tctggtatgg cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga acaatctgat
     4621 gttgctcgaa tatgccgggg agcgaatgct ctctcacatc gttgccgagc acggcgacta
     4681 ccaggcgacc gaaattgcag cggaactaat ggcgaagctg tatgccgcat ctgaggaacc
     4741 cctgccttct gcccttctcc cgatccggga tcgctttgca gctttgtttc agcgggcgcg
     4801 cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac gcggcgatta tagccgatca
     4861 aatgatgagc aatgcctcgg aactgcgtgg gctacatggc gatctgcatc atgaaaacat
     4921 catgttctcc agtcgcggct ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg
     4981 ctttggcgcc gccaatatgt tctacgatcc ggctgacaga gacgaccttt gtctcgatcc
     5041 tagacgcatt gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg
     5101 cctgctcgac caggcgtacg cttatgggtg cctttccgca gcttggaacg cggatggaga
     5161 agaggagcaa cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac agacgtcata
     5221 ctagatatca agcgacttct cctatcccct gggaacacat caatctcacc ggagaatatc
     5281 gctggccaaa gccttagcgt aggattccgc cccttcccgc aaacgacccc aaacaggaaa
     5341 cgcagctgaa acgggaagct caacacccac tgacgcatgg gttgttcagg cagtacttca
     5401 tcaaccagca aggcggcact ttcggccatc cgccgcgccc cacagctcgg gcagaaaccg
     5461 cgacgcttac agctgaaagc gaccaggtgc tcggcgtggc aagactcgca gcgaacccgt
     5521 agaaagccat gctccagccg cccgcattgg agaaattctt caaattcccg ttgcacatag
     5581 cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta atactcgtca
     5641 acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc agtatcccga
     5701 tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc aatactgtgt
     5761 ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa tgcctgccgt
     5821 tgctagacat tgccagccag tgcccgtcac tcccactctt acattgttcg cagtatctag
     5881 gtacatgtta ttgcgtgcac cttcatgatg tataatgtta ttaatgacag cagaaagatc
     5941 gacagaatta atattcttcg tacctgggac agttatcggg tgtcggccag tgatgagacc
     6001 aaaggatgca gtataagatg aaccactact attatcatgg ccaccattac caccaccgtt
     6061 aaaactacct cccgaacttc cagagccgct atgaccatca ttgtaggggt catttggtcc
     6121 catgtgacca aaaccaccga cagtaccgct atttaactct gtactcataa aattatcctc
     6181 tttgactttt aaaaacagca aaaactgtat ttataacaat gtgtttatat acagtattcg
     6241 aaatactacg cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt ttcacgcctc
     6301 acgggcagcc tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt acaatacggt
     6361 acacacagga gacactgtca cttttatggc atggcacaca gttcaagaga cactgaaatt
     6421 aacaggaaag tcgcgctcgc aattatatcg tgacatggct aaaggccttg tcagctatcg
     6481 cacaggaaaa aatgatcgcc gggagttcga gaccagtgaa ctgattcggg cctatggcga
     6541 gctgcgcact tctgagacac caaaaaggca caaggaggga cactccaaat ctgtcgttag
     6601 ccaggcggaa aatgctcagc tagacgtcat tcagcagaaa ctggacgctc tgcagcagac
     6661 tgttacccta atgcttgaag acaagatggc gcgggaagca gaaaaccgcc tgcgaaatga
     6721 ggagagggaa caactacagg ccgaaattag ccgtctggct gacgcgct
//
