LOCUS       NZ_AP027691             1943 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSS-10 plasmid pPSS-10_6, complete sequence.
ACCESSION   NZ_AP027691
VERSION     NZ_AP027691.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562535
            Assembly: GCF_036501505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 159x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036501505.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:10:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,072
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,638
            CDSs (with protein)               :: 4,638
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 310
            CDSs (without protein)            :: 310
            Pseudo Genes (ambiguous residues) :: 0 of 310
            Pseudo Genes (frameshifted)       :: 83 of 310
            Pseudo Genes (incomplete)         :: 226 of 310
            Pseudo Genes (internal stop)      :: 46 of 310
            Pseudo Genes (multiple problems)  :: 42 of 310
            Pseudo Genes (short protein)      :: 3 of 310
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027691.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSS-10"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSS-10_6"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            1625..1738
                     /locus_tag="AABK11_RS25345"
     CDS             1625..1738
                     /locus_tag="AABK11_RS25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517211.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          1251..1738
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.858"
                     /coverage="93.0"
                     /evalue="7.55e-176"
ORIGIN
        1 gaaatctaac gagtcagtgt cgagctagaa gatggtgagg acagagtaat acttgaatta
       61 gtgcagctag atgattcaac ggtattaatc attctatcaa ggcgaaccat acatttctca
      121 acgacaagcc cacctctacc atcactgcta atgttggtca caaatggacc tgcttggctt
      181 gagcagccag caagaattac tgatgacaga aaaaccaatg agagaattac ctttttcata
      241 aaagccccag aaaatagaga agtaggaata atcttatctt ttaatttgtt aatcaacata
      301 aacgatattt accataacat ctcttgcccg caccgctgaa aacgcctcag agccattttg
      361 aggcgaacgg gccgtttccg gcacctttcc cagcaccaac ccctgaaaaa cgcgcctgat
      421 gatttctgcg catctgcatg gctatgcacg aacatgcctg gccggaattt tagcggctaa
      481 aatcctgcga actgctcaaa aggctcgctg aaccccatcg ataacggtga acgggtgcac
      541 agtttactgt gcggtcgtga ggcgttgagc aacgagcttt gcgaggcggt aggggttctg
      601 agagccagct gcaggctcag gagaaaacat tctgttttcg gggcagatac cgcaaatgac
      661 gtcattttgc ggcgtctgcc ctatcctgca tcgcaacgtc taaaggatgt gagcacaaac
      721 gccagcactt tgcgaatcct gccctcgatc tgaacaaaag caaaatttcg tacgtacgaa
      781 attcagggta tcaggctgcg cctgatgaaa tgtttttggt gccggcggta ctccaaaaac
      841 acatcttgtc cgacagtttt gtggtgccgg cgaagcggcg gtggccacaa aacatttcct
      901 gtccccatcg attcattcga gaaacataag aggcgaagaa ggggtcagtt tggagatcgg
      961 aaatttttgt acgttaagcg cattatttaa ccaatcttgg ttcgtaaggg gttgattttg
     1021 cgtcagtggc tatcgctttt aagttgtcgc tcttgcagta tcgcatttag gttatccgat
     1081 taaggtcaaa cctctgaaaa tgccgtatag cgcgggagta caccctattg ctataggtaa
     1141 gtctgttcaa aaaacaggct taccgtacaa taattctcta tatccaaact gacccctggc
     1201 aggtgaaaaa tgtagctgta gggcgtaagt gaaaaatgca ggagtcgatg aaattacgcc
     1261 tcctcgctca ctcggtcgct acgctccgga cgtgagactg cggcgggcgt taccggctca
     1321 gaagtaaagg agtaggctag tagcaatcaa attgggaaag gaagagaaaa ggaagcggcg
     1381 ttgccgtttt tccataggct ccgcccccct gacaagcatc acaaaatctg acgctcaaat
     1441 cagtggcggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     1501 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     1561 cgtttatctc attccacgcc tgacactcgg ttccgggaag gcagttcgct ccaagctgga
     1621 ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc tgttccggta actatcgact
     1681 tgagtccaac ccggaaagac atgcaaaagc gccactggca gcagccactg gtaattgatt
     1741 tagaggagtt agtcttgaag ttatgcgccg gttaaggcta aactgaaagg acaagttttg
     1801 gtgactgtgc tcctccaagc cagttacctc ggttcaaaga gttggtagct cagagaacct
     1861 tcgaaaaacc gccctgcaag gcggtttttt ctttttcaag cgaaagacta cccgctgaat
     1921 taaataatct caagtagcaa cat
//
