LOCUS       NZ_AP027670             9092 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSS-04 plasmid pPSS-04_4, complete sequence.
ACCESSION   NZ_AP027670
VERSION     NZ_AP027670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562521
            Assembly: GCF_036500705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 150x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036500705.1-RS_2024_04_03
            Annotation Date                   :: 04/03/2024 23:58:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,746
            CDSs (total)                      :: 4,625
            Genes (coding)                    :: 4,365
            CDSs (with protein)               :: 4,365
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 56 of 260
            Pseudo Genes (incomplete)         :: 189 of 260
            Pseudo Genes (internal stop)      :: 54 of 260
            Pseudo Genes (multiple problems)  :: 38 of 260
            Pseudo Genes (short protein)      :: 3 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027670.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSS-04"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSS-04_4"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            join(8399..9092,1..4)
                     /locus_tag="AABJ69_RS23640"
     CDS             join(8399..8648,8648..9092,1..4)
                     /locus_tag="AABJ69_RS23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(118..411)
                     /locus_tag="AABJ69_RS23645"
     CDS             complement(118..411)
                     /locus_tag="AABJ69_RS23645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123874500.1"
                     /translation="MFEEQRFLAGEKQFCKRWYIDMSSFGINPGKTPGNKRNIPDVTYF
                     HFCQQHPPPTPENKKAACSIGKPYIKGNYVLRFVLNRDALLLRITNQIIHVL"
                     /name="hypothetical protein"
     gene            complement(1279..1470)
                     /locus_tag="AABJ69_RS23650"
                     /old_locus_tag="VEE17_45450"
     CDS             complement(1279..1470)
                     /locus_tag="AABJ69_RS23650"
                     /old_locus_tag="VEE17_45450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2401..>3265)
                     /locus_tag="AABJ69_RS23655"
                     /pseudo=""
     CDS             complement(2401..>3265)
                     /locus_tag="AABJ69_RS23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            3269..3661
                     /locus_tag="AABJ69_RS23660"
     CDS             3269..3661
                     /locus_tag="AABJ69_RS23660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            3799..4683
                     /locus_tag="AABJ69_RS23665"
                     /old_locus_tag="VEE17_45490"
     CDS             3799..4683
                     /locus_tag="AABJ69_RS23665"
                     /old_locus_tag="VEE17_45490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     ARG             complement(4716..5989)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.57"
                     /coverage="100.0"
     ARG             complement(4719..5914)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            6020..6670
                     /gene="tetR(A)"
                     /locus_tag="AABJ69_RS23675"
                     /old_locus_tag="VEE17_45510"
     CDS             6020..6670
                     /gene="tetR(A)"
                     /locus_tag="AABJ69_RS23675"
                     /old_locus_tag="VEE17_45510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_038989485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEARPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(6702..6947)
                     /locus_tag="AABJ69_RS23680"
     CDS             complement(6702..6947)
                     /locus_tag="AABJ69_RS23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041937891.1"
                     /translation="MSDFRALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(<6927..7298)
                     /locus_tag="AABJ69_RS23685"
                     /pseudo=""
     CDS             complement(<6927..7298)
                     /locus_tag="AABJ69_RS23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            complement(7302..7862)
                     /locus_tag="AABJ69_RS23690"
                     /old_locus_tag="VEE17_45530"
     CDS             complement(7302..7862)
                     /locus_tag="AABJ69_RS23690"
                     /old_locus_tag="VEE17_45530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            complement(8237..>8344)
                     /locus_tag="AABJ69_RS23695"
                     /pseudo=""
     CDS             complement(8237..>8344)
                     /locus_tag="AABJ69_RS23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="class A beta-lactamase"
     Typing          1346..1965
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.243"
                     /coverage="99.0"
                     /evalue="1.1799999999999999e-174"
ORIGIN
        1 gtaagttgga gtcattacca ccgaaatgat gtgaagactt catcatttta gcaatcacag
       61 gttgccctaa tcacttaacg ctattattgt tctattgaag ccttaaccta aacccactca
      121 caaaacgtgg attatctggt tagtgattcg cagcaacaaa gcgtcccggt tcaaaacgaa
      181 tcgtaagacg tagtttccct tgatgtaggg cttgccgatg gaacaggcgg cttttttatt
      241 ctccggcgtg ggggggggat gctgttgaca gaaatggaaa taagtaacat ccggaatatt
      301 acgcttattg cccggcgtct tgcctggatt aatgccaaaa gaactcatat cgatatacca
      361 tcgtttgcag aactgctttt ctccggccaa aaagcgctgt tcttcgaaca atataattat
      421 tttcactcac gacggtacac aacggcaggc acgtcaaagc caccggattt accataacat
      481 ccgttgcccg caccgcctca gaggccctca gggcgtttct gaggcctccg gcctgaaaca
      541 cacacctttc tgtgcccata tcgagaaaaa acgcccctga tgattctggc gcgactgcat
      601 ggctatgcac ggacatacct ggccgggatt ttagcggcta aaatccgatg aacgccctga
      661 aagccaccat gaaccacccc gataacggtg aacggatgcg caatttattg cgtggccgtg
      721 aggcgttgcc ggcacagccg gaaaaaccat tttcagcacg tgcaggaggc ccaggaagag
      781 ggggcggatg tacgaatagt tcagaggtca gatctgacgt aaaaatgatg taaaagtgac
      841 gtaaaagtga tgtacttttg atgtaattct gttgtacacc cgcgatctga cgtaaaagtg
      901 atgtaatttt gatgtacttt tgatgtaatt ctgttgtaca cccgcgttct ggggtaaaag
      961 cagatgcaat tttgtagttt atgtgtagtc gctgaaccca tagggtcata cgagcatgac
     1021 cctttcccgg aaaaggacgc tttttagcca gaatcatcct taacctgcgg cgccagcact
     1081 cgggagtggt cgttttatat actaaaaaat gacatacacc taacgcagct gcgattttca
     1141 tcgcaacttc atgaaaaata aataaaaaag aaaaaataaa gggattctgg gtattaccgg
     1201 ggtaataata ttaagcacca agacaatgaa aattaaggcg atgccggtat tactccggta
     1261 atacccacaa cggagccatt acgcaccgtc ctgctgctca ttcaggcgcg ctgacaggct
     1321 tcggtaaagg cgttccgcat cctcatggag tttctcgcag aaatcagcct catgatccag
     1381 atccagctca ttaagcctgt ccagaagaag cagggactga tcctggatga atttagccat
     1441 gttaacggct gtcttttgtt gtttgttcat gaaggccccg gcgcgaaagt gcttactgta
     1501 cgttctgatg gttttccgct tcctcgctca ttcgctcact ccgttcggtc atgagtctgc
     1561 ggcgagcgga gggaagataa gcagataacg taacggtaag cataacgaaa gctaaggaca
     1621 agggcgaaaa ggccacgccc cagcggggcg tttttccata ggctccgccc ccctgacaag
     1681 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     1741 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     1801 ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg
     1861 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg
     1921 cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa atgcgccact
     1981 ggcagcagcc actggtaaca ggattcacgg agcagagtag tagagtgctt ctacagagtt
     2041 cttgaagtgg tggcctgact gcggctacac tagaaggaca gttttggtat ctgcgctccg
     2101 tgaaagccag ttaccttcgg aaaaaagagt tggtagctct tgattccggc aaacgaacca
     2161 ccgttggtag cggtggtttt tttgtttgca ggacatgaga ttacgacgca acagaaagga
     2221 tctcaagaag ttccttgctg tattgctact atcctagaga tctgacccat taggggcatt
     2281 atcagattac ggccttgtaa tccacgataa aaacccagaa accagaagag ttggcccagc
     2341 gacatatgtt acataaacgc ccacataggg gagcccgcag aattcggaaa aaatcgtacg
     2401 ctaaggtttt ccgggcatcc gtaagggccg aaacttcccg tcttccagtc tgcggctctg
     2461 ccgccagacg taatcgccgg ttaggttgat gtgctcccag cccagcggcg acaggaattg
     2521 cagcagctcg ccgtccaccg gcttgccggc ctcgaccaac ccctgggtgg cgcgttccag
     2581 gtacaccgtg ttccacagca cgatagccgc cgtcaccagg ttgaggccgc tggcccggta
     2641 gcgctgctgc tcgaagctcc gatccctgat ttccccaagg cggttgaaga acaccgccct
     2701 ggccagcgag ttgcgcgcct cacctttgtt caggccggca tgcacgcggc ggcgcagttc
     2761 aacactttgc agccagtcca ggatgaacag cgtgcgctcg atccggcacc gccgcatcca
     2821 gcggggtgat tttcagccct gactcggtga ggatggcatc gggcagctcg ttgtccttgg
     2881 ccaggcgggt gacggtggcc aactgctcgt ccagcagctg caaacgctct tccaggtact
     2941 ggtcgctgtt cgggttgatc gccaggggca gggcctgctc gcgcttgagt gcggcgaact
     3001 tctcggccgg cagcaggtag tcgtcgaagt cgcggaactg ccgcgagccc ttgacccaga
     3061 tgtcgccgga gcgcagggcg ttcttcagct cggacagggc gcagatttcg tagaatttcc
     3121 ggtcgaggcc ttccggggtg atcaccagcg gcttccagcg cggcttgatg aaggccgtgg
     3181 gtgcatcggc cggcaccttg cgcaggttgt cggcgttcat ctcacgcagg gtctgcacgg
     3241 ctgccagcac gccttgcgcg gccggcccgt gctgttcccc aacttttcgc tcggcgaaga
     3301 gtatgaacat gcgccgccgg cgacgaatcg ccaaatctca ccgtatctcc cgagcggacg
     3361 atttcgcacc ggtctgcccg tcgaagggct tgcgatcgaa cggggcgacc ttttctatgc
     3421 atgtccgcga gccagcgtct tttatggcac ggcgctcgac gccgaccttc ggacgcgcgg
     3481 cgtaagcacg cttgtcatgg ccgggataag caccaccggc gttgttcttt caagcgtcgc
     3541 ctgggctagt gatgcggact acgacgtgcg tttggtccag gactgctgct acgacccgga
     3601 tcgggatgcc cacgaagctc tgttgcgttc cgggttcggc ggacgtgtac aggtcgtgta
     3661 attttcgggc ctcgcatgat cgcggccata gccgcaggtg gattggtgcg aggcagggcc
     3721 agtccaagtc agggtgcggt catcgcggcg cctgcaactc tcaaaaaccc atcgccgtag
     3781 cggaggtctt gtaatcgcat gtcattacgc acgcccgatt tattgttcac agcgatagca
     3841 cctgccattt ggggcagcac ctacattgtc accacccaat acctgccgaa cttctcaccg
     3901 atgacggtcg cgatgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga
     3961 cagattccaa cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg
     4021 ctattctgga gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg
     4081 gtaggcgctg tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg
     4141 atacgattga tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg
     4201 ttgacaccaa acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt
     4261 tccatggcgt tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc
     4321 tttaccgcct ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt
     4381 gatccgccaa tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg
     4441 atcggagcgg gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca
     4501 gttgtttcct tactgggctt tctcagcccg gggaccgccg tgttgctagg atggttgttc
     4561 ttggatcaga cgctgagtgc gcttcaaatc atcggcgtcc tgctcgtgat cgggagtatc
     4621 tggctgggcc aacgttccaa ccgcactcct agggcgcgta tagcttgccg gaagtcgcct
     4681 tgacccgcat ggcataggcc tatcgtttcc acgatcagcg atcggctcgt tgccctgcgc
     4741 cgctccaaag cccgcgacgc agcgccggca ggcagagcaa gtagagggca gcgcctgcaa
     4801 tccatgccca cccgttccac gttgttatag aagccgcata gatcgccgtg aagaggaggg
     4861 gtccgacgat cgaggtcagg ctggtgagcg ccgccagtga gccttgcagc tgcccctgac
     4921 gttcctcatc cacctgcctg gacaacattg cttgcagcgc cggcattccg atgccacccg
     4981 aagcaagcag gaccatgatc gggaacgcca tccatccccg tgtcgcgaag gcaagcagga
     5041 tgtagcctgt gccgtcggca atcattccga gcatgagtgc ccgcctttcg ccgagccggg
     5101 cggctacagg gccggtgatc attgcctggg cgagtgaatg cagaatgcca aatgcggcaa
     5161 gcgaaatgcc gatcgtggtc gcgtcccagt gaaagcgatc ctcgccgaaa atgacccaaa
     5221 gcgcggccgg cacctgtccg acaagttgca tgatgaagaa gaccgccatc agggcggcga
     5281 cgacggtcat gccccgggcc caccggaacg aagcgagcgg gttgagagcc tcccggcgta
     5341 acggccggcg ttcgcctttg tgcgactccg gcaaaaggaa acagcccgtc aggaaattga
     5401 ggccgttcaa ggctgccgcg gcgaagaacg gagcgtgggg ggagaaatcg cccatcagcc
     5461 caccgagcac aggtcccgcg accatcccga acccgaaaca ggcgctcatg aagccgaagt
     5521 gccgcgcgcg ctcatcgcca tcagtgatat cggcaatata agcgccggct accgccccag
     5581 tcgccccggt gatgccggcc acgatccgcc cgatatagag aacccaaagg aaaggcgccg
     5641 tcgccatgat ggcgtagtcg acagcagcgc cggccagcga gacgagcaag accggccgcc
     5701 gcccgaaacg atccgacagc gcgcccagca caggtgcgca ggcaaattgc atcaacgcat
     5761 acagcgccag cagaatgcca tagtgggcgg tgacgtcgtt cgagtgaacc agatcgcgca
     5821 ggaggcccgg cagcaccggc ataatcaggc cgatgccgac agcgtcgagc gcgacagtgc
     5881 tcagaattac gatcaggggt ctgttgggtt tcacgtctgg cctccggacc agcctccgct
     5941 ggtccgattg aacgcgcgga ttctttatca ctgataagtt ggtggacata ttatgtttat
     6001 cagtgataaa gtgtcaagca tgacaaagtt gcagccgaat acagtgatcc gtgccgccct
     6061 ggacctgttg aacgaggtcg gcgtagacgg tctgacgaca cgcaaactgg cggaacggtt
     6121 gggggttcag cagccggcgc tttactggca cttcaggaac aagcgggcgc tgctcgacgc
     6181 actggccgaa gccatgctgg cggagaatca tacgcattcg gtgccgagag ccgacgacga
     6241 ctggcgctca tttctgatcg ggaatgcccg cagcttcagg caggcgctgc tcgcctaccg
     6301 cgatggcgcg cgcatccatg ccggcacgcg accgggcgca ccgcagatgg aaacggccga
     6361 cgcgcagctt cgcttcctct gcgaggcggg tttttcggcc ggggacgccg tcaatgcgct
     6421 gatgacaatc agctacttca ctgttggggc cgtgcttgag gagcaggccg gcgacagcga
     6481 tgccggcgag cgcggcggca ccgttgaaca ggctccgctc tcgccgctgt tgcgggccgc
     6541 gatagacgcc ttcgacgaag cccgtccgga cgcagcgttc gagcagggac tcgcggtgat
     6601 tgtcgatgga ttggcgaaaa ggaggctcgt tgtcaggaac gttgaaggac cgagaaaggg
     6661 tgacgattga tcaggaccgc tgccggagcg caacccactc actacagcag agccatgtag
     6721 acaacatccc ctcccccttt ccaccgcgtc agacgcccgt agcagcccgc tacgggcttt
     6781 ttcatgccct gccctagcgt ccaagcctca cggccgcgct cggcctctct ggcggccttc
     6841 tggcgctcct gctgcggcgt ccgctcgtgg gccgtggcgc gggtccgcgc gccggcctcg
     6901 tgcgcctggc gctcgcgggc gaggtccagg gcgcggaagt cggacaggcc gaacggggcc
     6961 agttgcaggt aggtgcgcag ttcctgggca tgctcgcgac gggtcacgtc gcgctcgccg
     7021 tactttgccc agctcgccgg ctcggcctgg acttgcttcg ccacccacag gatgaccggc
     7081 tcgggcagct cgctgtcggt tcccagcgcg taaccggggt agcgcagcag gcagagctgc
     7141 acggcgaagc cgaggcggtt ggcgtcgccg cgtcgctggc ggatcagcga caggtcggag
     7201 tcgttgaagg tgtagtagcg gatcaggtca tcctggcttt ccggcagcgc aagcagggtg
     7261 tcccgctccg tggccgagag gatcaagcga cgcggcatgt gtcagtcgtc cgtgcggagg
     7321 tactggtaga gggtttcccg gctgatgttg aactcgcggg caagctgcgc cttgggctcg
     7381 ccggccgtcg ctcgctgccg cagggtagca gcctgctcat cggacagggc tttcttgcgg
     7441 ccccggtacg cgccacgctg cttggccaag gcgatgccct cacgctgccg ctcgcggatc
     7501 agggcgcgct cgaactcagc gaaggccccc atcaccgaca gcatcaggtt ggccatcggc
     7561 gagtcctcgc cagtgaacac caggccctcc ttcaggaact cgatgcgcac gccgcgctga
     7621 gtcagcttct gtaccaagcg acgcaggtca tcgaggttgc gggccagccg atccatgctg
     7681 tgcaccacca ctgtatcgcc ttcgcggacg aagctcagca gcgcttcgag ctgggggcgc
     7741 tgggtgtcct tgcccgatgc cttgtcggtg aacaccttgc tcacctgggt ttgttccagc
     7801 tggcgttccg ggttctggtc aaagctgctg acccggacgt agccgatgcg gtgcccctgc
     7861 acgatgtctc cttggttgaa ggcggcttaa gtgcactttc tgttccgttg tgcctcaaag
     7921 cccatttctg tcaggctgaa atctataacc ttcgcgggca tgtgtcaaaa aatgggaaag
     7981 cagactctat tctgacgaag cggcgcggcc ctgcctgaca tcaagttagg gtatagccta
     8041 gattgacatg cgcgatgcaa cccttaactt gcttgcacct atcgtttccg gggtctgacg
     8101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8341 gctggtgatg atgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
     8401 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
     8461 caaaagcact gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact
     8521 gcagttcact tacaccgctt ctcaacccgg tacgcaccag aaaatcattg atatggccat
     8581 gaatggcgtt ggatgccggg caaccgcccg cattatgggc gttggcctca acacgatttt
     8641 ccgccattta aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac
     8701 gtcatcgtct gcgcggaaat ggacgaacag tggggatacg tcggggctaa atcgcgccag
     8761 cgctggctgt tttacgcgta tgacaggctc cggaagacgg ttgttgcgca cgtattcggt
     8821 gaacgcacta tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg
     8881 atatggatga cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta
     8941 atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg
     9001 gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc
     9061 atcgggcatt atctgaacat aaaacactat ca
//
