LOCUS       NZ_AP027665             1506 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-42 plasmid pPSJ-42_13, complete
            sequence.
ACCESSION   NZ_AP027665
VERSION     NZ_AP027665.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562565
            Assembly: GCF_036500485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 135x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036500485.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,040
            CDSs (total)                      :: 4,922
            Genes (coding)                    :: 4,743
            CDSs (with protein)               :: 4,743
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 78 of 179
            Pseudo Genes (incomplete)         :: 115 of 179
            Pseudo Genes (internal stop)      :: 32 of 179
            Pseudo Genes (multiple problems)  :: 41 of 179
            Pseudo Genes (short protein)      :: 2 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027665.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-42"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-42_13"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            1..441
                     /locus_tag="AABK18_RS25200"
                     /old_locus_tag="VEE39_48190"
     CDS             1..441
                     /locus_tag="AABK18_RS25200"
                     /old_locus_tag="VEE39_48190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          complement(727..983)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="98.0"
                     /evalue="3.06e-107"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagttggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccggattta aagagtcagc ggcaggcgga caatcagggc tacgtgttaa
      481 cgttttgacc atgattgtct atcctgcatt gctcttttgc cgcatgtcag ttcgatgcga
      541 tgcaggatag ggcaaacgcc gcaaaatgac gtcattgacg tcattcgcaa cctttgcccg
      601 ataatctcca aaacgtctga gctgccacgg ttccggcagc gccttgagcg caaccggaaa
      661 ccgtctgggg agaaccccag acccccatcg ctgtaacatc gacggtctac gaccaccaac
      721 cagctgaata gctgccagtg ccgtgcaaat gggctttgca gggggctgac gcggttcctg
      781 gcgacctgaa agcccctggt taggggtatc ggcgcgcctg acctcctgaa accctctgta
      841 agcgtttttc gcgccttcca tgccctgacg ggcatttaaa acccgtttcg tgcgcctgag
      901 agcttctgag agcgtttgaa gagctatctg gcgactcatc cccgaaagaa caacagcgga
      961 atcaccgata acatgcgaga ggtggcaccg ctcgccgcag ccccagcgac cgaacgttag
     1021 tgagcgagtg ggcgaggaag cggaagagaa ccggaaacca cattgagcac gtacgcactg
     1081 atgcggggtg tcggggtgaa gccctgacca gatggcaaac ggaatatcgt cgcgggtgac
     1141 ggtattacat ttgcacatcc tgtcccgatt tctgcggagt ttaacggtga acggacggca
     1201 aaaaacgtgc aggagctgtt gaacgtgaaa acctcgaaat ctggcaaaaa tcacggctga
     1261 cgaggcttgc agaggattaa gccagtatac actccgctat cgttacgtga ctgggtcagg
     1321 gcttcgcccc gacaccccaa agagtagcta ggataaatat ctaatggaat caatgcgtta
     1381 gacgcctaat ataaagatac agaaaggaga ataaaaacat taacaaaatc taagggttac
     1441 agttgacata gttatcaata atgataacac tgttatcatt attgataact caaatagggt
     1501 tcaaag
//
