LOCUS       NZ_AP027663             2313 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-42 plasmid pPSJ-42_11, complete
            sequence.
ACCESSION   NZ_AP027663
VERSION     NZ_AP027663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562565
            Assembly: GCF_036500485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 135x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036500485.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,040
            CDSs (total)                      :: 4,922
            Genes (coding)                    :: 4,743
            CDSs (with protein)               :: 4,743
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 78 of 179
            Pseudo Genes (incomplete)         :: 115 of 179
            Pseudo Genes (internal stop)      :: 32 of 179
            Pseudo Genes (multiple problems)  :: 41 of 179
            Pseudo Genes (short protein)      :: 2 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027663.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-42"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-42_11"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            910..1335
                     /gene="mobA"
                     /locus_tag="AABK18_RS25180"
                     /old_locus_tag="VEE39_48150"
     CDS             910..1335
                     /gene="mobA"
                     /locus_tag="AABK18_RS25180"
                     /old_locus_tag="VEE39_48150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_024257935.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRLASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLYELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDIYNEMDLIKDEIKIIKRLHKNKYPGSDLFK"
                     /name="mobA"
     gene            complement(1369..1866)
                     /locus_tag="AABK18_RS25185"
                     /old_locus_tag="VEE39_48160"
     CDS             complement(1369..1866)
                     /locus_tag="AABK18_RS25185"
                     /old_locus_tag="VEE39_48160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_023156898.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNVTQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 ggggtctaaa tgcccgtcag ggcatggaag gcgcgaaaaa cgcttacaga gggtttcagg
       61 aggccaggcg cgccgatacc cctaaccagg ggctttcagg tcgccaggaa ccgcgccagc
      121 cccctgcaaa gcccatttgc acgggatggg cagctactcg gttggtgggt ggtcgcagac
      181 cgatgatgtt gcagcggtgg gggtctgggg ttctccccag acggtttccg gttgcgctca
      241 aggcgctgcc ggaaccgtgg cgactcggta ggtttgaagc gcgtttccgg cgttcgttgg
      301 cacgcaaacc atccccatgg ttagttcttg ccggaaacgc gccctgatgt tctatcgtat
      361 cgatggttat atgtcgaggt tgtcagcagc tggcggagca ctgcacgggg attaatgctc
      421 ccattgccag gatgaaataa agattaggag cgacgttaag ttatattttt aatatataaa
      481 cgcgatatca ataactcaca tagttatatt ttatgcatat gctaccatct tgctcttttg
      541 cctcctgctc tctggacgaa tcccgcatta ccaacaggca atggattata taaccaagaa
      601 gatctaatct ttattcctct aataatccac aaacgacaga caacaaagac atcaattaat
      661 tcggtgttat ttattaaata acaagcaata atttttaaaa tctgtgtaaa taatattgaa
      721 tgaagcatca tttttttgta tcagaaatac caggttacga cgcggtattt tattttcgtt
      781 gaaaagttgc agcggaaaat gggacaggat gtgtattcgt cataccgcga atacattcgc
      841 ttgcatgaga atacctcctg tcagggcaag ccccgacacc ccaaacgtgc aaaatggaaa
      901 gtcattgcga tggctaaaaa taaacgtagt ggttcagaag tacggcagag aactaaggtt
      961 atcaccctta gagtaaataa cagactcgca agcgaaatca gacggcgagc gaagaacgaa
     1021 ggactgacaa tttcagaata tatcaggacg gcaagtttaa ataatgaaat aaaacagcgc
     1081 gtaccttcca ggtatttata tgaattaatc cgacttggtc ggatgcagaa gaaattattt
     1141 gataagggaa agcggccgaa ggataaagag tacctcgagg tgatgcataa aataatttta
     1201 ctttgcgatg aaatgaaaat agtaacgaag cgaatttctg atatatataa tgaaatggat
     1261 ttaataaagg atgaaataaa aataataaaa aggctacata aaaataaata tccaggttcg
     1321 gatttattca aataaggtct ttttaaaaag accttattta ggtgcgcatt attttatttc
     1381 ttcggttgtt ataatagttt caccgttagg tgaatattta attctcattt caaactcgcg
     1441 acgctgttca attatttttt tccattcacc acgagcaaat aaattagggt tcgcctgata
     1501 ttcattaccg ccacaactag caataatccc catttttgat aatgaataca atctgttacg
     1561 taatgttccg tcttttattc ccaataattt acagatctcc ttacggtatc tagttgataa
     1621 tgcaatatat ccgtcataat ccaatttcct aagaattaaa aataaaacat cttttagaga
     1681 atcaggtgct tttgtgacag cacataaatt atcaatataa attttcacat acgcaggttc
     1741 ttgtggaaat tgaactgtta tatcggttat ttcttgtgtt acgtttccag tttctctatc
     1801 tataacctca gtaatatttt gtcttaaaac ttttttcttg ctatccgttc tttcagtaat
     1861 gttcatttta tcgcctttgt cactttataa aggtgacaaa caatataata ccccaaaaac
     1921 aaaatgtcac tcaatataga ttacgcgaat accgcccaca cgttacaggt gtatcctgta
     1981 cacgttacag gtgtatcttc tacacgttac aacattggcg atcagcccgt atgtagccta
     2041 attcggatat cttatatttt taaatgtatc cacaagcacg aggcataatc tctatttacg
     2101 aagtgacaat tttaatattg agcgtgcgtg ctcaatgtag cttccggttc tcttccgctt
     2161 cctcgcccac tcgcgagcct ggcggctcgg tcgctggggc tgcggcgagc ggtgccacct
     2221 ctcgcatatc gttgatgatc ccgatttgat ctcaggcgat cgagtcgctg gtcatctgtt
     2281 aaacgcgctc agagcctccc agacgcacga aac
//
