LOCUS       NZ_AP027656             6647 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-42 plasmid pPSJ-42_4, complete sequence.
ACCESSION   NZ_AP027656
VERSION     NZ_AP027656.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562565
            Assembly: GCF_036500485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 135x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036500485.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:14:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,040
            CDSs (total)                      :: 4,922
            Genes (coding)                    :: 4,743
            CDSs (with protein)               :: 4,743
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 78 of 179
            Pseudo Genes (incomplete)         :: 115 of 179
            Pseudo Genes (internal stop)      :: 32 of 179
            Pseudo Genes (multiple problems)  :: 41 of 179
            Pseudo Genes (short protein)      :: 2 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027656.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-42"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-42_4"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            37..195
                     /locus_tag="AABK18_RS24970"
     CDS             37..195
                     /locus_tag="AABK18_RS24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            693..920
                     /locus_tag="AABK18_RS24975"
                     /old_locus_tag="VEE39_47800"
     CDS             693..920
                     /locus_tag="AABK18_RS24975"
                     /old_locus_tag="VEE39_47800"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(917..2485)
                     /gene="cea"
                     /locus_tag="AABK18_RS24980"
                     /old_locus_tag="VEE39_47810"
     CDS             complement(917..2485)
                     /gene="cea"
                     /locus_tag="AABK18_RS24980"
                     /old_locus_tag="VEE39_47810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2745..3182
                     /locus_tag="AABK18_RS24985"
                     /old_locus_tag="VEE39_47820"
     CDS             2745..3182
                     /locus_tag="AABK18_RS24985"
                     /old_locus_tag="VEE39_47820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021532742.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSKGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3263..3682
                     /locus_tag="AABK18_RS24990"
                     /old_locus_tag="VEE39_47830"
     CDS             3263..3682
                     /locus_tag="AABK18_RS24990"
                     /old_locus_tag="VEE39_47830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4000..4233)
                     /locus_tag="AABK18_RS24995"
                     /old_locus_tag="VEE39_47840"
     CDS             complement(4000..4233)
                     /locus_tag="AABK18_RS24995"
                     /old_locus_tag="VEE39_47840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4240..4758)
                     /locus_tag="AABK18_RS25000"
                     /old_locus_tag="VEE39_47850"
     CDS             complement(4240..4758)
                     /locus_tag="AABK18_RS25000"
                     /old_locus_tag="VEE39_47850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4796..5494)
                     /locus_tag="AABK18_RS25005"
                     /pseudo=""
     CDS             complement(<4796..5494)
                     /locus_tag="AABK18_RS25005"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5436..5759)
                     /locus_tag="AABK18_RS25010"
                     /old_locus_tag="VEE39_47860"
     CDS             complement(5436..5759)
                     /locus_tag="AABK18_RS25010"
                     /old_locus_tag="VEE39_47860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5823..6014
                     /locus_tag="AABK18_RS25015"
                     /old_locus_tag="VEE39_47870"
     CDS             5823..6014
                     /locus_tag="AABK18_RS25015"
                     /old_locus_tag="VEE39_47870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6100..6187)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..381
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.206"
                     /coverage="86.0"
                     /evalue="8.92e-71"
     Typing          6198..6647
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.469"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3897..5446)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
       61 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag gcacgcctta
      121 acgccactgg cagcagccac tggtaaccgg attagcagag cgatgatggc acaaacggtg
      181 ctacagagtt cttgaagtag tggcccgact acggctacac tagaaggaca gtatttggta
      241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
      301 aacaaaccac cgttggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
      361 aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttt
      421 agaagaggag gatggtgcga tggtccctcc ctgaacatca cgtatatagt tagcctgaca
      481 tccaacaagg aggtttatcg cgaatattcc cacaaaaaat cttttcctca taactcgatc
      541 cttataaaat gaaaagaata tatggcgagg tttaatttat gagcttaaga tactacataa
      601 aaaatatttt atttggcctg tactgcacac ttatatatat ataccttata acaaaaaaca
      661 gcgaagggta ttatttcctt gtgtcagata agatgctata tgcaatagtg ataagcacta
      721 ttctatgtcc atattcaaaa tatgctattg aatacatagc ttttaacttc ataaagaaag
      781 attttttcga aagaagaaaa aacctaaata acgcccccgt agcaaaatta aacctattta
      841 tgctatataa tctactttgt ttggtcctag caatcccatt tggattgcta ggacttttta
      901 tatcaataaa gaataattaa atccctaaca cctcatttat agtattaagt ttattcttat
      961 caatatagga gcatagaatt cctgtaacaa tagcaatacc ccaaatacct aatgtagttc
     1021 cagcaagcaa gctaaaaagt aaagcaacaa cataactcac ccctgcatct gcagctttct
     1081 tctctaatgt aagaaatagt ggcttccagt cacctgtatc cttaattttt aggatatcag
     1141 ataccacatc atatccaaaa gaaacatgcc ccgtaatctt caagtacttg gcaaactgat
     1201 ctaaatgttt agcccagtca tcatacttca ccgatgccaa cgcattaaaa atagcatcac
     1261 gatcggcttt gctgaatttc ttatttaaaa catccttgta tttttcaaaa gcagcgagag
     1321 cttcattcac attgccgatt ttcttacctt tagacttatc agcaagttcc tgtgccattt
     1381 tcgaatattt ttcaccatat ttttcagtca gcgtttgata aaagctaact gttgcatcaa
     1441 cagcatcctt aatctgtgaa ttaaggagat tattctgtgc ttttttcaaa ttttcttcag
     1501 cttcatgaac acgagcgata ccggcattac gattattact gacctgagaa atagccttct
     1561 ggatctgagt tatatcagca tttatccggt taatacgtgt ttctgatgct gttacctgtt
     1621 tttgtttttc ttctctaatc ttaccggccc caacccgtcg tctggttgct tcaaaaaaag
     1681 gacggttctg aagcggatca ttggctcttg gtgatagttt tttgaccagc tcatccagtt
     1741 ctttatattt agcggatgcc tgagccagtt catttcgttt tccagcgagc gttttcattt
     1801 ctgcatcacg ggcatggata ctggagctta aacgagaatt gagagtctta atctctccat
     1861 ccattttcac cacttcagat tgtgcagcag aaagtttttt ttgggcgatc tcaacagctt
     1921 tagcttcttc actcaatgca gccagtcgtt tctcttcagc ttcagccagt ttcaactggc
     1981 gttctgtttc agccttctcc cgttcaatct ctttacgtcg ttgttctgct tcctgaaaag
     2041 ccttttctgc tgcttccgct tctttacggg ctttttcttc tgctttcgca aggcgcaaac
     2101 gctctgcttc cgcctgcata gctgcattat tagcatgagc aagctctgtt gctgaaggcg
     2161 tacgtgaggc attgtgacga agagcctcat tcacgatatc cttcaggcgc tgagtcagcg
     2221 catccctgtt tgcctttgct ttcgcctgtg cttccgctgc agcttttgcc cgggcagcct
     2281 gctctgcctg tgttttcttt aattgagcag tagaccattt agcagttgca tgaatagctg
     2341 cagaactttc acttttactg cctccttttc cacctccgcc gccagagcca ctcccgtcag
     2401 gagtaccatt caaaagagta ataattacct gtcccttatc atcataagga acaccatctt
     2461 tatagtacgc taccgcggtt tccattataa aatcctcttt gacttttaaa acaataagtt
     2521 aaaaataaat actgtacata taaccactgg ttttatatac agcataaaag ctacgccgct
     2581 gcattttccc tgtcaagact gtggacttcc atttttgtga aaacgatcaa aaaaacagtc
     2641 tttcacacca cgcgctattc tcgcccgatg ccacaaaaac cagcacaaac attaccgttc
     2701 tcagacctca ttatgtttta ctgaaactat gagatgagac atctatggga cactgtcact
     2761 ttatggcatg gcacacactc cgggacgcac taaaaatgac aggcagatcg cgttcacagt
     2821 tttaccgtga tatgcgcgca ggccttgtca gttaccgtac cggcagggac ggacgacggg
     2881 agtttgaaac cagtgaactg atccgggcat acggcgaatt aaagcagaat gagacaccag
     2941 aaaggcacag taagggacat gcagaaaatc cacatgatca gcagacagaa cgcattctcc
     3001 gggagctgaa tgagctgaaa caatgcctga cgctgatgct tgaggataaa caggcacagg
     3061 atatggatcg cagacgccag gaagcagaac gggaacagct acaaaatgag atagcccagc
     3121 tcaggcaggc actggaactg gaaaagaaac ggggattctg gtccaggttg ttcggtcgct
     3181 gaacgctgtc agagactgat gataaaatag tcttcggata ataactcacc gagaataaat
     3241 actttaaggt agggagacac tcatgagacg taccggaaac aaactttgtc ttatcgccat
     3301 gataacagca acagtagctc tcacagcctg taccccaaag ggcagcgtgg aacaacatac
     3361 ccggcattac gtatatgctt ctgatgacgg ttttgatccc aacttttcca cccaaaaagc
     3421 cgacacaaca cgaatgatgg tgcctttttt tcggcagttc tgggatatgg gagctaaaga
     3481 taaagcgaca ggaaaatcac ggagtgatgt gcaacaacgc attcagcagt ttcacagcca
     3541 ggaattttta aactcactcc ggggcacaac tcaatttgcg ggtactgatt accgcagcaa
     3601 agaccttacc ccgaaaaaat ccaggctgct ggctgacacg atttctgcgg tttatctcga
     3661 tggctacgag ggcagacagt aagtggattt accataatcc cttaattgta cgcaccgcta
     3721 aaacgcgttc agcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     3781 aactgccgcg atcgcgcctg ataaatttta accgtatgaa tacctatgca accagagggt
     3841 acaggccaca ttacccccac ttaatccact gaagctgcca tttttcatgg tttcaccatc
     3901 ccagcgaagg gccatccagc gtgcgttcct gtatttccgg ctgacgctcc cgttccaggg
     3961 ataacacatg ttcgcgctcc tgtatcagcc gttcctctct tatctccagt tctcgctgta
     4021 taactggctc aagcgttctg tctgctcgct caagtgctgc acctgctgac tcaactgcat
     4081 gacccgctcg ttcagcatcg cgttgtcccg ttgcgtaagc gaaaacatct tctgcaattc
     4141 cacgaaggcg ctctcccatt cgctcagccg ctgcatatag tcctgttgca gctgctctaa
     4201 ggcgttcagc aaatgtgttt ccagctctgt cactctgtgt cactccttca gatgtaccca
     4261 ctctttcccc tgaaagggaa tcacctccgc tgatttcccg tacggaagga caaggaattt
     4321 cctgttcccg tcctgcacaa actccacgcc ccatgtcttc gcgttcagtt tctgcaatgt
     4381 ctcttcctgc ttcctgattt cttccaggtt cgcctgtatc ctccctccaa gataccagag
     4441 cgtcccgcca ctcgcggtaa acaggagaaa gactatcccc agtaacatca tgcccgtatt
     4501 ccctgccagc tttaacacgt ccctcctgtg ctgcatcatc gcctctttca ccccttcccg
     4561 gtgtttttcc agcgattcct ctgtcgaggc tgtgaacagg gctatagcgt ctctgatttt
     4621 cgtctcgttt gatgtcacag cctcgcttac agattcgccg agcctcctga actcgttgtt
     4681 cagcattttc tctgtagatt cggctctctc tttcagcttt ttctcgaact ccgcgcccgt
     4741 ctgcaaaaga ttgctcataa aatgctcctt tcagcctgat attcttcccg ccgttcggat
     4801 ctgcaatgct gatactgctt cgcgtcaccc tgaccacttc cagccccgcc tcagtgagcg
     4861 cctgaatcac atcctgacgg ccttttatct ctccggcatg gtaaagtgca tctatacctc
     4921 gcgtgacgcc ctcagcaagc gcctgtttcg tttcaggcag gttatcaggg agtgtcagcg
     4981 tcctgcggtt ctccggggcg ttcgggtcat gcagcccgta atggtgattc accagcgtct
     5041 gccaggcatc aattctaggc ctgtctgcgc ggtcgtagta cggctggagg cgttttccgg
     5101 tctgtagctc catgttcgga atgacaaaat tcagctcaag ccgtcccttg tcctggtgct
     5161 ccacccacag gatgctgtac tgattttttt cgagaccggg catcagtaca cgctcaaagc
     5221 tcgccatcac tttttcacgt cctcccggcg gcagctcctt ctccgcgaac gacagaacac
     5281 cggacgtgta tttcttcgca aatggcgtgg catcgatgag ttcccggact tcttccggat
     5341 taccctgaag caccgttgcg ccttcgcggt tacgctccct ccccagcagg taatcaaccg
     5401 gaccactgcc accacctttt cccctggcat gaaatttaac tatcatcccg cgccccctgt
     5461 tccctgacag ccagacgcag ccggcgcagc tcatccccga tggccatcag tgcggccacc
     5521 acctgaaccc ggtcaccgga agaccactgc ccgctgttca ccttacgggc tgtctgattc
     5581 aggttatttc cgatggcggc cagctgacgc agtaacggcg gtgccagtgt cggcagtttt
     5641 ccggaacggg caaccggctc ccccaggcag acccgccgca tccataccgc cagttgttta
     5701 ccctcacagc gttcaagtaa ccgggcatgt tcatcatcag taacccgtat tgtgagcatc
     5761 ctctcgcgtt tcatcggtat cattacccca tgaacagaaa tcccccttac acggaggcat
     5821 cagtgactaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     5881 taacgctgct ggagaagctc aacgaactgg acgcagatga acaggccgat atttgtgaat
     5941 cgcttcacga ccacgccgat gagctttacc gcagctgcct cgcacgtttc ggggatgacg
     6001 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg tgagcggatg
     6061 ccgggagctg acaagcccgt cagggcgcgt cagcaggttt tagcgggtgt cggggcgcag
     6121 ccctgaccca gtcacgtagc gatagcggag tgtatactgg cttaaccatg cggcatcagt
     6181 gcggattgta tgaaaagtac gccatgccgg gtgtgaaatg ccgcacagat gcgtaaggag
     6241 aaaatgcacg tccaggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt
     6301 tcgactgcgg cgagcggtac tgactcacac aaaaacggta acacagttat ccacagaatc
     6361 aggggataag gccggaaaga acatgtgagc aaaagaccag gaacaggaag aaggccacgt
     6421 agcaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat agacgctcaa
     6481 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6541 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6601 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctc
//
