LOCUS       NZ_AP027651             9092 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-36 plasmid pPSJ-36_3, complete sequence.
ACCESSION   NZ_AP027651
VERSION     NZ_AP027651.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562561
            Assembly: GCF_036500285.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 374x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036500285.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:07:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,896
            CDSs (total)                      :: 4,775
            Genes (coding)                    :: 4,613
            CDSs (with protein)               :: 4,613
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 49 of 162
            Pseudo Genes (incomplete)         :: 98 of 162
            Pseudo Genes (internal stop)      :: 36 of 162
            Pseudo Genes (multiple problems)  :: 22 of 162
            Pseudo Genes (short protein)      :: 3 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027651.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-36"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-36_3"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            228..788
                     /locus_tag="AABK04_RS24420"
                     /old_locus_tag="VEE37_46810"
     CDS             228..788
                     /locus_tag="AABK04_RS24420"
                     /old_locus_tag="VEE37_46810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            792..>1163
                     /locus_tag="AABK04_RS24425"
                     /pseudo=""
     CDS             792..>1163
                     /locus_tag="AABK04_RS24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            1143..1388
                     /locus_tag="AABK04_RS24430"
     CDS             1143..1388
                     /locus_tag="AABK04_RS24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041937891.1"
                     /translation="MSDFRALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(1420..2070)
                     /gene="tetR(A)"
                     /locus_tag="AABK04_RS24435"
                     /old_locus_tag="VEE37_46830"
     CDS             complement(1420..2070)
                     /gene="tetR(A)"
                     /locus_tag="AABK04_RS24435"
                     /old_locus_tag="VEE37_46830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_038989485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEARPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     ARG             2102..3375
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.57"
                     /coverage="100.0"
     ARG             2177..3372
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            complement(3407..4291)
                     /locus_tag="AABK04_RS24445"
                     /old_locus_tag="VEE37_46850"
     CDS             complement(3407..4291)
                     /locus_tag="AABK04_RS24445"
                     /old_locus_tag="VEE37_46850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     gene            complement(4429..4821)
                     /locus_tag="AABK04_RS24450"
     CDS             complement(4429..4821)
                     /locus_tag="AABK04_RS24450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            <4825..5689
                     /locus_tag="AABK04_RS24455"
                     /pseudo=""
     CDS             <4825..5689
                     /locus_tag="AABK04_RS24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            6620..6811
                     /locus_tag="AABK04_RS24460"
                     /old_locus_tag="VEE37_46890"
     CDS             6620..6811
                     /locus_tag="AABK04_RS24460"
                     /old_locus_tag="VEE37_46890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6949..7452
                     /locus_tag="AABK04_RS24465"
     CDS             6949..7452
                     /locus_tag="AABK04_RS24465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152925299.1"
                     /translation="MKIAAALGVCHFLVYKTTTPECWRRRLRMILAKKRPFPGKGHARM
                     TLWVQRLHINYKIASAFTPERGCTTELHQKYIKITSLLRQIAGVQQNYIKSTSLLRHFY
                     IIFTSDLTSELFVHPPPLPGPPARAENGFSGCAGNASRPRNKLRIRSPLSGWFMVAFRA
                     FIGF"
                     /name="hypothetical protein"
     gene            7679..7972
                     /locus_tag="AABK04_RS24470"
     CDS             7679..7972
                     /locus_tag="AABK04_RS24470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123874500.1"
                     /translation="MFEEQRFLAGEKQFCKRWYIDMSSFGINPGKTPGNKRNIPDVTYF
                     HFCQQHPPPTPENKKAACSIGKPYIKGNYVLRFVLNRDALLLRITNQIIHVL"
                     /name="hypothetical protein"
     gene            complement(8086..8783)
                     /locus_tag="AABK04_RS24475"
     CDS             complement(join(8086..8534,8534..8783))
                     /locus_tag="AABK04_RS24475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            <8838..8945
                     /locus_tag="AABK04_RS24480"
                     /pseudo=""
     CDS             <8838..8945
                     /locus_tag="AABK04_RS24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="class A beta-lactamase"
     Typing          complement(6126..6745)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.243"
                     /coverage="99.0"
                     /evalue="1.1799999999999999e-174"
ORIGIN
        1 ggaaacgata ggtgcaagca agttaagggt tgcatcgcgc atgtcaatct aggctatacc
       61 ctaacttgat gtcaggcagg gccgcgccgc ttcgtcagaa tagagtctgc tttcccattt
      121 tttgacacat gcccgcgaag gttatagatt tcagcctgac agaaatgggc tttgaggcac
      181 aacggaacag aaagtgcact taagccgcct tcaaccaagg agacatcgtg caggggcacc
      241 gcatcggcta cgtccgggtc agcagctttg accagaaccc ggaacgccag ctggaacaaa
      301 cccaggtgag caaggtgttc accgacaagg catcgggcaa ggacacccag cgcccccagc
      361 tcgaagcgct gctgagcttc gtccgcgaag gcgatacagt ggtggtgcac agcatggatc
      421 ggctggcccg caacctcgat gacctgcgtc gcttggtaca gaagctgact cagcgcggcg
      481 tgcgcatcga gttcctgaag gagggcctgg tgttcactgg cgaggactcg ccgatggcca
      541 acctgatgct gtcggtgatg ggggccttcg ctgagttcga gcgcgccctg atccgcgagc
      601 ggcagcgtga gggcatcgcc ttggccaagc agcgtggcgc gtaccggggc cgcaagaaag
      661 ccctgtccga tgagcaggct gctaccctgc ggcagcgagc gacggccggc gagcccaagg
      721 cgcagcttgc ccgcgagttc aacatcagcc gggaaaccct ctaccagtac ctccgcacgg
      781 acgactgaca catgccgcgt cgcttgatcc tctcggccac ggagcgggac accctgcttg
      841 cgctgccgga aagccaggat gacctgatcc gctactacac cttcaacgac tccgacctgt
      901 cgctgatccg ccagcgacgc ggcgacgcca accgcctcgg cttcgccgtg cagctctgcc
      961 tgctgcgcta ccccggttac gcgctgggaa ccgacagcga gctgcccgag ccggtcatcc
     1021 tgtgggtggc gaagcaagtc caggccgagc cggcgagctg ggcaaagtac ggcgagcgcg
     1081 acgtgacccg tcgcgagcat gcccaggaac tgcgcaccta cctgcaactg gccccgttcg
     1141 gcctgtccga cttccgcgcc ctggacctcg cccgcgagcg ccaggcgcac gaggccggcg
     1201 cgcggacccg cgccacggcc cacgagcgga cgccgcagca ggagcgccag aaggccgcca
     1261 gagaggccga gcgcggccgt gaggcttgga cgctagggca gggcatgaaa aagcccgtag
     1321 cgggctgcta cgggcgtctg acgcggtgga aagggggagg ggatgttgtc tacatggctc
     1381 tgctgtagtg agtgggttgc gctccggcag cggtcctgat caatcgtcac cctttctcgg
     1441 tccttcaacg ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag
     1501 tccctgctcg aacgctgcgt ccggacgggc ttcgtcgaag gcgtctatcg cggcccgcaa
     1561 cagcggcgag agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc
     1621 ggcctgctcc tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac
     1681 ggcgtccccg gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc
     1741 catctgcggt gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag
     1801 cagcgcctgc ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc
     1861 tctcggcacc gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag
     1921 cgcccgcttg ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc
     1981 cagtttgcgt gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg
     2041 gatcactgta ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat
     2101 atgtccacca acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg
     2161 gtccggaggc cagacgtgaa acccaacaga cccctgatcg taattctgag cactgtcgcg
     2221 ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg
     2281 gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttgatg
     2341 caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccggtc
     2401 ttgctcgtct cgctggccgg cgctgctgtc gactacgcca tcatggcgac ggcgcctttc
     2461 ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta
     2521 gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc
     2581 atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc
     2641 gatttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg
     2701 acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag
     2761 gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg
     2821 atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt
     2881 ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt
     2941 ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc
     3001 gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc
     3061 ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc
     3121 ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag
     3181 ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc
     3241 acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct
     3301 gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa
     3361 cgagccgatc gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc
     3421 ggcaagctat acgcgcccta ggagtgcggt tggaacgttg gcccagccag atactcccga
     3481 tcacgagcag gacgccgatg atttgaagcg cactcagcgt ctgatccaag aacaaccatc
     3541 ctagcaacac ggcggtcccc gggctgagaa agcccagtaa ggaaacaact gtaggttcga
     3601 gtcgcgagat cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc
     3661 acgccaggcc gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag
     3721 ctactggaac gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag
     3781 gcacgggagg ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg
     3841 ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg
     3901 ccacgcccgc agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta
     3961 gcagagcggc agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga
     4021 ccccgcccgg caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa
     4081 gtgcgccgag gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca
     4141 tcacgagcaa taaacccgcc ggcaacgccc gcagcatcgc gaccgtcatc ggtgagaagt
     4201 tcggcaggta ttgggtggtg acaatgtagg tgctgcccca aatggcaggt gctatcgctg
     4261 tgaacaataa atcgggcgtg cgtaatgaca tgcgattaca agacctccgc tacggcgatg
     4321 ggtttttgag agttgcaggc gccgcgatga ccgcaccctg acttggactg gccctgcctc
     4381 gcaccaatcc acctgcggct atggccgcga tcatgcgagg cccgaaaatt acacgacctg
     4441 tacacgtccg ccgaacccgg aacgcaacag agcttcgtgg gcatcccgat ccgggtcgta
     4501 gcagcagtcc tggaccaaac gcacgtcgta gtccgcatca ctagcccagg cgacgcttga
     4561 aagaacaacg ccggtggtgc ttatcccggc catgacaagc gtgcttacgc cgcgcgtccg
     4621 aaggtcggcg tcgagcgccg tgccataaaa gacgctggct cgcggacatg catagaaaag
     4681 gtcgccccgt tcgatcgcaa gcccttcgac gggcagaccg gtgcgaaatc gtccgctcgg
     4741 gagatacggt gagatttggc gattcgtcgc cggcggcgca tgttcatact cttcgccgag
     4801 cgaaaagttg gggaacagca cgggccggcc gcgcaaggcg tgctggcagc cgtgcagacc
     4861 ctgcgtgaga tgaacgccga caacctgcgc aaggtgccgg ccgatgcacc cacggccttc
     4921 atcaagccgc gctggaagcc gctggtgatc accccggaag gcctcgaccg gaaattctac
     4981 gaaatctgcg ccctgtccga gctgaagaac gccctgcgct ccggcgacat ctgggtcaag
     5041 ggctcgcggc agttccgcga cttcgacgac tacctgctgc cggccgagaa gttcgccgca
     5101 ctcaagcgcg agcaggccct gcccctggcg atcaacccga acagcgacca gtacctggaa
     5161 gagcgtttgc agctgctgga cgagcagttg gccaccgtca cccgcctggc caaggacaac
     5221 gagctgcccg atgccatcct caccgagtca gggctgaaaa tcaccccgct ggatgcggcg
     5281 gtgccggatc gagcgcacgc tgttcatcct ggactggctg caaagtgttg aactgcgccg
     5341 ccgcgtgcat gccggcctga acaaaggtga ggcgcgcaac tcgctggcca gggcggtgtt
     5401 cttcaaccgc cttggggaaa tcagggatcg gagcttcgag cagcagcgct accgggccag
     5461 cggcctcaac ctggtgacgg cggctatcgt gctgtggaac acggtgtacc tggaacgcgc
     5521 cacccagggg ttggtcgagg ccggcaagcc ggtggacggc gagctgctgc aattcctgtc
     5581 gccgctgggc tgggagcaca tcaacctaac cggcgattac gtctggcggc agagccgcag
     5641 actggaagac gggaagtttc ggcccttacg gatgcccgga aaaccttagc gtacgatttt
     5701 ttccgaattc tgcgggctcc cctatgtggg cgtttatgta acatatgtcg ctgggccaac
     5761 tcttctggtt tctgggtttt tatcgtggat tacaaggccg taatctgata atgcccctaa
     5821 tgggtcagat ctctaggata gtagcaatac agcaaggaac ttcttgagat cctttctgtt
     5881 gcgtcgtaat ctcatgtcct gcaaacaaaa aaaccaccgc taccaacggt ggttcgtttg
     5941 ccggaatcaa gagctaccaa ctcttttttc cgaaggtaac tggctttcac ggagcgcaga
     6001 taccaaaact gtccttctag tgtagccgca gtcaggccac cacttcaaga actctgtaga
     6061 agcactctac tactctgctc cgtgaatcct gttaccagtg gctgctgcca gtggcgcatt
     6121 tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc agcggtcgga
     6181 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
     6241 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     6301 aggcaggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg tatctttata
     6361 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg
     6421 ggcggagcct atggaaaaac gccccgctgg ggcgtggcct tttcgccctt gtccttagct
     6481 ttcgttatgc ttaccgttac gttatctgct tatcttccct ccgctcgccg cagactcatg
     6541 accgaacgga gtgagcgaat gagcgaggaa gcggaaaacc atcagaacgt acagtaagca
     6601 ctttcgcgcc ggggccttca tgaacaaaca acaaaagaca gccgttaaca tggctaaatt
     6661 catccaggat cagtccctgc ttcttctgga caggcttaat gagctggatc tggatcatga
     6721 ggctgatttc tgcgagaaac tccatgagga tgcggaacgc ctttaccgaa gcctgtcagc
     6781 gcgcctgaat gagcagcagg acggtgcgta atggctccgt tgtgggtatt accggagtaa
     6841 taccggcatc gccttaattt tcattgtctt ggtgcttaat attattaccc cggtaatacc
     6901 cagaatccct ttattttttc ttttttattt atttttcatg aagttgcgat gaaaatcgca
     6961 gctgcgttag gtgtatgtca ttttttagta tataaaacga ccactcccga gtgctggcgc
     7021 cgcaggttaa ggatgattct ggctaaaaag cgtccttttc cgggaaaggg tcatgctcgt
     7081 atgaccctat gggttcagcg actacacata aactacaaaa ttgcatctgc ttttacccca
     7141 gaacgcgggt gtacaacaga attacatcaa aagtacatca aaattacatc acttttacgt
     7201 cagatcgcgg gtgtacaaca gaattacatc aaaagtacat cacttttacg tcacttttac
     7261 atcattttta cgtcagatct gacctctgaa ctattcgtac atccgccccc tcttcctggg
     7321 cctcctgcac gtgctgaaaa tggtttttcc ggctgtgccg gcaacgcctc acggccacgc
     7381 aataaattgc gcatccgttc accgttatcg gggtggttca tggtggcttt cagggcgttc
     7441 atcggatttt agccgctaaa atcccggcca ggtatgtccg tgcatagcca tgcagtcgcg
     7501 ccagaatcat caggggcgtt ttttctcgat atgggcacag aaaggtgtgt gtttcaggcc
     7561 ggaggcctca gaaacgccct gagagcctct cagacggtgc acgcaacgga tgttatggta
     7621 aatccggtgg ctttgacgtg cctgccgttg tgtaccgtcg tgagtgaaaa taattatatt
     7681 gttcgaagaa cagcgctttt tggccggaga aaagcagttc tgcaaacgat ggtatatcga
     7741 tatgagttct tttggcatta atccaggcaa gacgccgggc aataagcgta atattccgga
     7801 tgttacttat ttccatttct gtcaacagca tccccccccc acgccggaga ataaaaaagc
     7861 cgcctgttcc atcggcaagc cctacatcaa gggaaactac gtcttacgat tcgttttgaa
     7921 ccgggacgct ttgttgctgc gaatcactaa ccagataatc cacgttttgt gagtgggttt
     7981 aggttaaggc ttcaatagaa caataatagc gttaagtgat tagggcaacc tgtgattgct
     8041 aaaatgatga agtcttcaca tcatttcggt ggtaatgact ccaacttact gatagtgttt
     8101 tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag
     8161 cgacttccgt cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg
     8221 ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg
     8281 ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggctca taagacgccc
     8341 cagcgtcgcc atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc ggagcctgtc
     8401 atacgcgtaa aacagccagc gctggcgcga tttagccccg acgtatcccc actgttcgtc
     8461 catttccgcg cagacgatga cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg
     8521 cctgagtttt ttaaatggcg gaaaatcgtg ttgaggccaa cgcccataat gcgggcggtt
     8581 gcccggcatc caacgccatt catggccata tcaatgattt tctggtgcgt accgggttga
     8641 gaagcggtgt aagtgaactg cagttgccat gttttacggc agtgagagca gagatagcgc
     8701 tgatgtccgg cagtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga
     8761 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
     8821 taagttggca tcatcaccag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8881 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8941 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9001 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9061 gtgagttttc gttccactga gcgtcagacc cc
//
