LOCUS       NZ_AP027645             9092 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-32-1 plasmid pPSJ-32-1_3, complete
            sequence.
ACCESSION   NZ_AP027645
VERSION     NZ_AP027645.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562569
            Assembly: GCF_036500085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 245x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036500085.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:19:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,682
            CDSs (total)                      :: 4,561
            Genes (coding)                    :: 4,302
            CDSs (with protein)               :: 4,302
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 259
            CDSs (without protein)            :: 259
            Pseudo Genes (ambiguous residues) :: 0 of 259
            Pseudo Genes (frameshifted)       :: 57 of 259
            Pseudo Genes (incomplete)         :: 188 of 259
            Pseudo Genes (internal stop)      :: 53 of 259
            Pseudo Genes (multiple problems)  :: 38 of 259
            Pseudo Genes (short protein)      :: 3 of 259
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027645.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-32-1"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-32-1_3"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            join(8442..9092,1..47)
                     /locus_tag="AABJ87_RS23320"
     CDS             join(8442..8691,8691..9092,1..47)
                     /locus_tag="AABJ87_RS23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(161..454)
                     /locus_tag="AABJ87_RS23325"
     CDS             complement(161..454)
                     /locus_tag="AABJ87_RS23325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123874500.1"
                     /translation="MFEEQRFLAGEKQFCKRWYIDMSSFGINPGKTPGNKRNIPDVTYF
                     HFCQQHPPPTPENKKAACSIGKPYIKGNYVLRFVLNRDALLLRITNQIIHVL"
                     /name="hypothetical protein"
     gene            complement(1322..1513)
                     /locus_tag="AABJ87_RS23330"
                     /old_locus_tag="VEE41_44720"
     CDS             complement(1322..1513)
                     /locus_tag="AABJ87_RS23330"
                     /old_locus_tag="VEE41_44720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2444..>3308)
                     /locus_tag="AABJ87_RS23335"
                     /pseudo=""
     CDS             complement(2444..>3308)
                     /locus_tag="AABJ87_RS23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            3312..3704
                     /locus_tag="AABJ87_RS23340"
     CDS             3312..3704
                     /locus_tag="AABJ87_RS23340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="WP_001351729.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIER
                     GDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRLVQ
                     DCCYDPDRDAHEALLRSGFGGRVQVV"
                     /name="isochorismatase family cysteine hydrolase"
     gene            3842..4726
                     /locus_tag="AABJ87_RS23345"
                     /old_locus_tag="VEE41_44760"
     CDS             3842..4726
                     /locus_tag="AABJ87_RS23345"
                     /old_locus_tag="VEE41_44760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="WP_000058717.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPA
                     GLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVV
                     FISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLT
                     RKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYF
                     LWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSN
                     RTPRARIACRKSP"
                     /name="EamA family transporter"
     ARG             complement(4759..6032)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="98.57"
                     /coverage="100.0"
     ARG             complement(4762..5957)
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="99.75"
                     /coverage="100.0"
     gene            6063..6713
                     /gene="tetR(A)"
                     /locus_tag="AABJ87_RS23355"
                     /old_locus_tag="VEE41_44780"
     CDS             6063..6713
                     /gene="tetR(A)"
                     /locus_tag="AABJ87_RS23355"
                     /old_locus_tag="VEE41_44780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_038989485.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEARPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            complement(6745..6990)
                     /locus_tag="AABJ87_RS23360"
     CDS             complement(6745..6990)
                     /locus_tag="AABJ87_RS23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_041937891.1"
                     /translation="MSDFRALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="hypothetical protein"
     gene            complement(<6970..7341)
                     /locus_tag="AABJ87_RS23365"
                     /pseudo=""
     CDS             complement(<6970..7341)
                     /locus_tag="AABJ87_RS23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            complement(7345..7905)
                     /locus_tag="AABJ87_RS23370"
                     /old_locus_tag="VEE41_44800"
     CDS             complement(7345..7905)
                     /locus_tag="AABJ87_RS23370"
                     /old_locus_tag="VEE41_44800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            complement(8280..>8387)
                     /locus_tag="AABJ87_RS23375"
                     /pseudo=""
     CDS             complement(8280..>8387)
                     /locus_tag="AABJ87_RS23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /name="class A beta-lactamase"
     Typing          1389..2008
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.243"
                     /coverage="99.0"
                     /evalue="1.1799999999999999e-174"
ORIGIN
        1 atgacaaagt catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta
       61 ccaccgaaat gatgtgaaga cttcatcatt ttagcaatca caggttgccc taatcactta
      121 acgctattat tgttctattg aagccttaac ctaaacccac tcacaaaacg tggattatct
      181 ggttagtgat tcgcagcaac aaagcgtccc ggttcaaaac gaatcgtaag acgtagtttc
      241 ccttgatgta gggcttgccg atggaacagg cggctttttt attctccggc gtgggggggg
      301 gatgctgttg acagaaatgg aaataagtaa catccggaat attacgctta ttgcccggcg
      361 tcttgcctgg attaatgcca aaagaactca tatcgatata ccatcgtttg cagaactgct
      421 tttctccggc caaaaagcgc tgttcttcga acaatataat tattttcact cacgacggta
      481 cacaacggca ggcacgtcaa agccaccgga tttaccataa catccgttgc ccgcaccgcc
      541 tcagaggccc tcagggcgtt tctgaggcct ccggcctgaa acacacacct ttctgtgccc
      601 atatcgagaa aaaacgcccc tgatgattct ggcgcgactg catggctatg cacggacata
      661 cctggccggg attttagcgg ctaaaatccg atgaacgccc tgaaagccac catgaaccac
      721 cccgataacg gtgaacggat gcgcaattta ttgcgtggcc gtgaggcgtt gccggcacag
      781 ccggaaaaac cattttcagc acgtgcagga ggcccaggaa gagggggcgg atgtacgaat
      841 agttcagagg tcagatctga cgtaaaaatg atgtaaaagt gacgtaaaag tgatgtactt
      901 ttgatgtaat tctgttgtac acccgcgatc tgacgtaaaa gtgatgtaat tttgatgtac
      961 ttttgatgta attctgttgt acacccgcgt tctggggtaa aagcagatgc aattttgtag
     1021 tttatgtgta gtcgctgaac ccatagggtc atacgagcat gaccctttcc cggaaaagga
     1081 cgctttttag ccagaatcat ccttaacctg cggcgccagc actcgggagt ggtcgtttta
     1141 tatactaaaa aatgacatac acctaacgca gctgcgattt tcatcgcaac ttcatgaaaa
     1201 ataaataaaa aagaaaaaat aaagggattc tgggtattac cggggtaata atattaagca
     1261 ccaagacaat gaaaattaag gcgatgccgg tattactccg gtaataccca caacggagcc
     1321 attacgcacc gtcctgctgc tcattcaggc gcgctgacag gcttcggtaa aggcgttccg
     1381 catcctcatg gagtttctcg cagaaatcag cctcatgatc cagatccagc tcattaagcc
     1441 tgtccagaag aagcagggac tgatcctgga tgaatttagc catgttaacg gctgtctttt
     1501 gttgtttgtt catgaaggcc ccggcgcgaa agtgcttact gtacgttctg atggttttcc
     1561 gcttcctcgc tcattcgctc actccgttcg gtcatgagtc tgcggcgagc ggagggaaga
     1621 taagcagata acgtaacggt aagcataacg aaagctaagg acaagggcga aaaggccacg
     1681 ccccagcggg gcgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     1741 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1801 tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     1861 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     1921 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     1981 atcgtcttga gtccaacccg gaaagacatg caaatgcgcc actggcagca gccactggta
     2041 acaggattca cggagcagag tagtagagtg cttctacaga gttcttgaag tggtggcctg
     2101 actgcggcta cactagaagg acagttttgg tatctgcgct ccgtgaaagc cagttacctt
     2161 cggaaaaaag agttggtagc tcttgattcc ggcaaacgaa ccaccgttgg tagcggtggt
     2221 ttttttgttt gcaggacatg agattacgac gcaacagaaa ggatctcaag aagttccttg
     2281 ctgtattgct actatcctag agatctgacc cattaggggc attatcagat tacggccttg
     2341 taatccacga taaaaaccca gaaaccagaa gagttggccc agcgacatat gttacataaa
     2401 cgcccacata ggggagcccg cagaattcgg aaaaaatcgt acgctaaggt tttccgggca
     2461 tccgtaaggg ccgaaacttc ccgtcttcca gtctgcggct ctgccgccag acgtaatcgc
     2521 cggttaggtt gatgtgctcc cagcccagcg gcgacaggaa ttgcagcagc tcgccgtcca
     2581 ccggcttgcc ggcctcgacc aacccctggg tggcgcgttc caggtacacc gtgttccaca
     2641 gcacgatagc cgccgtcacc aggttgaggc cgctggcccg gtagcgctgc tgctcgaagc
     2701 tccgatccct gatttcccca aggcggttga agaacaccgc cctggccagc gagttgcgcg
     2761 cctcaccttt gttcaggccg gcatgcacgc ggcggcgcag ttcaacactt tgcagccagt
     2821 ccaggatgaa cagcgtgcgc tcgatccggc accgccgcat ccagcggggt gattttcagc
     2881 cctgactcgg tgaggatggc atcgggcagc tcgttgtcct tggccaggcg ggtgacggtg
     2941 gccaactgct cgtccagcag ctgcaaacgc tcttccaggt actggtcgct gttcgggttg
     3001 atcgccaggg gcagggcctg ctcgcgcttg agtgcggcga acttctcggc cggcagcagg
     3061 tagtcgtcga agtcgcggaa ctgccgcgag cccttgaccc agatgtcgcc ggagcgcagg
     3121 gcgttcttca gctcggacag ggcgcagatt tcgtagaatt tccggtcgag gccttccggg
     3181 gtgatcacca gcggcttcca gcgcggcttg atgaaggccg tgggtgcatc ggccggcacc
     3241 ttgcgcaggt tgtcggcgtt catctcacgc agggtctgca cggctgccag cacgccttgc
     3301 gcggccggcc cgtgctgttc cccaactttt cgctcggcga agagtatgaa catgcgccgc
     3361 cggcgacgaa tcgccaaatc tcaccgtatc tcccgagcgg acgatttcgc accggtctgc
     3421 ccgtcgaagg gcttgcgatc gaacggggcg accttttcta tgcatgtccg cgagccagcg
     3481 tcttttatgg cacggcgctc gacgccgacc ttcggacgcg cggcgtaagc acgcttgtca
     3541 tggccgggat aagcaccacc ggcgttgttc tttcaagcgt cgcctgggct agtgatgcgg
     3601 actacgacgt gcgtttggtc caggactgct gctacgaccc ggatcgggat gcccacgaag
     3661 ctctgttgcg ttccgggttc ggcggacgtg tacaggtcgt gtaattttcg ggcctcgcat
     3721 gatcgcggcc atagccgcag gtggattggt gcgaggcagg gccagtccaa gtcagggtgc
     3781 ggtcatcgcg gcgcctgcaa ctctcaaaaa cccatcgccg tagcggaggt cttgtaatcg
     3841 catgtcatta cgcacgcccg atttattgtt cacagcgata gcacctgcca tttggggcag
     3901 cacctacatt gtcaccaccc aatacctgcc gaacttctca ccgatgacgg tcgcgatgct
     3961 gcgggcgttg ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat
     4021 ctggtggatg cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt
     4081 gtttatttcg gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc
     4141 gctgatggtc gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt
     4201 cctgggggct atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc
     4261 gctagatcct gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac
     4321 cgtgctgacc cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact
     4381 ggcggccgga ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat
     4441 gcctacagga accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac
     4501 ctacttcctt tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg
     4561 ctttctcagc ccggggaccg ccgtgttgct aggatggttg ttcttggatc agacgctgag
     4621 tgcgcttcaa atcatcggcg tcctgctcgt gatcgggagt atctggctgg gccaacgttc
     4681 caaccgcact cctagggcgc gtatagcttg ccggaagtcg ccttgacccg catggcatag
     4741 gcctatcgtt tccacgatca gcgatcggct cgttgccctg cgccgctcca aagcccgcga
     4801 cgcagcgccg gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc
     4861 cacgttgtta tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc
     4921 aggctggtga gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc
     4981 ctggacaaca ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg
     5041 atcgggaacg ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg
     5101 gcaatcattc cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg
     5161 atcattgcct gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg
     5221 gtcgcgtccc agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt
     5281 ccgacaagtt gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg
     5341 gcccaccgga acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct
     5401 ttgtgcgact ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc
     5461 gcggcgaaga acggagcgtg gggggagaaa tcgcccatca gcccaccgag cacaggtccc
     5521 gcgaccatcc cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg
     5581 ccatcagtga tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg
     5641 gccacgatcc gcccgatata gagaacccaa aggaaaggcg ccgtcgccat gatggcgtag
     5701 tcgacagcag cgccggccag cgagacgagc aagaccggcc gccgcccgaa acgatccgac
     5761 agcgcgccca gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc cagcagaatg
     5821 ccatagtggg cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc
     5881 ggcataatca ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg
     5941 ggtctgttgg gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc
     6001 ggattcttta tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa
     6061 gcatgacaaa gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg
     6121 tcggcgtaga cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg
     6181 cgctttactg gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc
     6241 tggcggagaa tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga
     6301 tcgggaatgc ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc
     6361 atgccggcac gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc
     6421 tctgcgaggc gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact
     6481 tcactgttgg ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg
     6541 gcaccgttga acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg
     6601 aagcccgtcc ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga
     6661 aaaggaggct cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat tgatcaggac
     6721 cgctgccgga gcgcaaccca ctcactacag cagagccatg tagacaacat cccctccccc
     6781 tttccaccgc gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc cctgccctag
     6841 cgtccaagcc tcacggccgc gctcggcctc tctggcggcc ttctggcgct cctgctgcgg
     6901 cgtccgctcg tgggccgtgg cgcgggtccg cgcgccggcc tcgtgcgcct ggcgctcgcg
     6961 ggcgaggtcc agggcgcgga agtcggacag gccgaacggg gccagttgca ggtaggtgcg
     7021 cagttcctgg gcatgctcgc gacgggtcac gtcgcgctcg ccgtactttg cccagctcgc
     7081 cggctcggcc tggacttgct tcgccaccca caggatgacc ggctcgggca gctcgctgtc
     7141 ggttcccagc gcgtaaccgg ggtagcgcag caggcagagc tgcacggcga agccgaggcg
     7201 gttggcgtcg ccgcgtcgct ggcggatcag cgacaggtcg gagtcgttga aggtgtagta
     7261 gcggatcagg tcatcctggc tttccggcag cgcaagcagg gtgtcccgct ccgtggccga
     7321 gaggatcaag cgacgcggca tgtgtcagtc gtccgtgcgg aggtactggt agagggtttc
     7381 ccggctgatg ttgaactcgc gggcaagctg cgccttgggc tcgccggccg tcgctcgctg
     7441 ccgcagggta gcagcctgct catcggacag ggctttcttg cggccccggt acgcgccacg
     7501 ctgcttggcc aaggcgatgc cctcacgctg ccgctcgcgg atcagggcgc gctcgaactc
     7561 agcgaaggcc cccatcaccg acagcatcag gttggccatc ggcgagtcct cgccagtgaa
     7621 caccaggccc tccttcagga actcgatgcg cacgccgcgc tgagtcagct tctgtaccaa
     7681 gcgacgcagg tcatcgaggt tgcgggccag ccgatccatg ctgtgcacca ccactgtatc
     7741 gccttcgcgg acgaagctca gcagcgcttc gagctggggg cgctgggtgt ccttgcccga
     7801 tgccttgtcg gtgaacacct tgctcacctg ggtttgttcc agctggcgtt ccgggttctg
     7861 gtcaaagctg ctgacccgga cgtagccgat gcggtgcccc tgcacgatgt ctccttggtt
     7921 gaaggcggct taagtgcact ttctgttccg ttgtgcctca aagcccattt ctgtcaggct
     7981 gaaatctata accttcgcgg gcatgtgtca aaaaatggga aagcagactc tattctgacg
     8041 aagcggcgcg gccctgcctg acatcaagtt agggtatagc ctagattgac atgcgcgatg
     8101 caacccttaa cttgcttgca cctatcgttt ccggggtctg acgctcagtg gaacgaaaac
     8161 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8221 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8281 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8341 gttgcctgac tccccgtcgt gtagataact acgatacggg agggctggtg atgatgccaa
     8401 cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca
     8461 gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc actgccggac
     8521 atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg
     8581 cttctcaacc cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc
     8641 gggcaaccgc ccgcattatg ggcgttggcc tcaacacgat tttccgccat ttaaaaaact
     8701 caggccgcag tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga
     8761 aatggacgaa cagtggggat acgtcggggc taaatcgcgc cagcgctggc tgttttacgc
     8821 gtatgacagg ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac
     8881 gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg
     8941 ctggccgctg tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac
     9001 gcagcgaatt gagcggcata acctgaatct gaggcagcac ctggcacggc tgggacggaa
     9061 gtcgctgtcg ttctcaaaat cggtggagct gc
//
