LOCUS       NZ_AP027631             1943 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-14 plasmid pPSJ-14_5, complete sequence.
ACCESSION   NZ_AP027631
VERSION     NZ_AP027631.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562563
            Assembly: GCF_036499205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 232x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036499205.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:04:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,972
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,610
            CDSs (with protein)               :: 4,610
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 72 of 244
            Pseudo Genes (incomplete)         :: 155 of 244
            Pseudo Genes (internal stop)      :: 57 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            Pseudo Genes (short protein)      :: 3 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027631.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-14"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-14_5"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            complement(1624..1737)
                     /locus_tag="AABJ92_RS24860"
     CDS             complement(1624..1737)
                     /locus_tag="AABJ92_RS24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517211.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(1621..1943)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.308"
                     /coverage="87.0"
                     /evalue="1.08e-148"
ORIGIN
        1 tgcttgtcag gggggcggag cctatggaaa aacggcaacg ccgcttcctt ttctcttcct
       61 ttcccaattt gattgctact agcctactcc tttacttctg agccggtaac gcccgccgca
      121 gtctcacgtc cggagcgtag cgaccgagtg agcgaggagg cgtaatttca tcgactcctg
      181 catttttcac ttacgcccta cagctacatt tttcacctgc caggggtcag tttggatata
      241 gagaattatt gtacggtaag cctgtttttt gaacagactt acctatagca atagggtgta
      301 ctcccgcgct atacggcatt ttcagaggtt tgaccttaat cggataacct aaatgcgata
      361 ctgcaagagc gacaacttaa aagcgatagc cactgacgca aaatcaaccc cttacgaacc
      421 aagattggtt aaataatgcg cttaacgtac aaaaatttcc gatctccaaa ctgacccctt
      481 cttcgcctct tatgtttctc gaatgaatcg atggggacag gaaatgtttt gtggccaccg
      541 ccgcttcgcc ggcaccacaa aactgtcgga caagatgtgt ttttggagta ccgccggcac
      601 caaaaacatt tcatcaggcg cagcctgata ccctgaattt cgtacgtacg aaattttgct
      661 tttgttcaga tcgagggcag gattcgcaaa gtgctggcgt ttgtgctcac atcctttaga
      721 cgttgcgatg caggataggg cagacgccgc aaaatgacgt catttgcggt atctgccccg
      781 aaaacagaat gttttctcct gagcctgcag ctggctctca gaacccctac cgcctcgcaa
      841 agctcgttgc tcaacgcctc acgaccgcac agtaaactgt gcacccgttc accgttatcg
      901 atggggttca gcgagccttt tgagcagttc gcaggatttt agccgctaaa attccggcca
      961 ggcatgttcg tgcatagcca tgcagatgcg cagaaatcat caggcgcgtt tttcaggggt
     1021 tggtgctggg aaaggtgccg gaaacggccc gttcgcctca aaatggctct gaggcgtttt
     1081 cagcggtgcg ggcaagagat gttatggtaa atatcgttta tgttgattaa caaattaaaa
     1141 gataagatta ttcctacttc tctattttct ggggctttta tgaaaaaggt aattctctca
     1201 ttggtttttc tgtcatcagt aattcttgct ggctgctcaa gccaagcagg tccatttgtg
     1261 accaacatta gcagtgatgg tagaggtggg cttgtcgttg agaaatgtat ggttcgcctt
     1321 gatagaatga ttaataccgt tgaatcatct agctgcacta attcaagtat tactctgtcc
     1381 tcaccatctt ctagctcgac actgactcgt tagatttcat gttgctactt gagattattt
     1441 aattcagcgg gtagtctttc gcttgaaaaa gaaaaaaccg ccctgcaagg cggtttttcg
     1501 aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcacagtca
     1561 ccaaaacttg tcctttcagt ttagccttaa ccggcgcata acttcaagac taactcctct
     1621 aaatcaatta ccagtggctg ctgccagtgg cgcttttgca tgtctttccg ggttggactc
     1681 aagtcgatag ttaccggaac aggcgcagca gtcggactga acggggggtt cgtgcataca
     1741 gtccagcttg gagcgaactg ccttcccgga accgagtgtc aggcgtggaa tgagataaac
     1801 gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga
     1861 gggagctacc agggggaaac gcctgggatc tttatagtcc tgtcgggttt cgccgccact
     1921 gatttgagcg tcagattttg tga
//
