LOCUS       NZ_AP027630             7127 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-14 plasmid pPSJ-14_4, complete sequence.
ACCESSION   NZ_AP027630
VERSION     NZ_AP027630.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562563
            Assembly: GCF_036499205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 232x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036499205.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:04:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,972
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,610
            CDSs (with protein)               :: 4,610
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 72 of 244
            Pseudo Genes (incomplete)         :: 155 of 244
            Pseudo Genes (internal stop)      :: 57 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            Pseudo Genes (short protein)      :: 3 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027630.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-14"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-14_4"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            1..840
                     /locus_tag="AABJ92_RS24815"
                     /old_locus_tag="VEE38_47920"
     CDS             1..840
                     /locus_tag="AABJ92_RS24815"
                     /old_locus_tag="VEE38_47920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_001517559.1"
                     /translation="MATHKPIDILASFTELPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGRVIYLPAEDPPVAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGNLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAISADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSGMTTGEAEE
                     WGVDDTQRPYFVRFGVSKANYGAPFKDRWFRRYDGGVLKPAVLEKQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1720
                     /gene="repC"
                     /locus_tag="AABJ92_RS24820"
                     /old_locus_tag="VEE38_47930"
     CDS             827..1720
                     /gene="repC"
                     /locus_tag="AABJ92_RS24820"
                     /old_locus_tag="VEE38_47930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_001669301.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRSLKRGERKRGKLDVVYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAV
                     QQDAMVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYA
                     SDEQDGKLYVALNPLIACAVMGGAQHVRIDMDEVRALQTDPARLMHQRLCGWIDPGKSG
                     RVELDTLCGYVWPGQATNPNTLKTRRQAARRALPELAALGWTVSEYAKNKFEIGRPKAT
                     HLLGPAPKTQEISHTA"
                     /name="repC"
     gene            1912..3066
                     /locus_tag="AABJ92_RS24825"
     CDS             1912..3066
                     /locus_tag="AABJ92_RS24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_252152372.1"
                     /translation="MTIANATVLVCLLPYISPFKTGLDSQPYGLIFIIISWLTLYKHQT
                     KHKHFMLLSLITFLLSSIILTVEAIIYGDTLSFIRGVYGYFVLFVAPFYFLTYLRFYDF
                     PKRTFYLSSSLYFIAAIIQAFVDVNFFDFLVDIRTSESRGVTSLTPEPTYYGIVCLLLL
                     MVCLVSESFKNKERKYLSLFFVIQIVFLAKSSMAILLLLILSFYFLCLKKISSTKILLG
                     LFLLLCGHFGILHLMEGSRVYDLYTRSIELGFYLFKIDASMNERLSHIYFSLYGMLNNA
                     FLPNFNSNFSTLLDNRYILSDGFFWYGESGKKIMSYWGGVFFNLGFIGVFYYIILASSV
                     KKGQLGVFLFVLTPILFTAVPMAMTIVSFLFSIFIFRSELRKPC"
                     /name="hypothetical protein"
     gene            complement(<3094..3210)
                     /locus_tag="AABJ92_RS24830"
                     /old_locus_tag="VEE38_47950"
                     /pseudo=""
     CDS             complement(<3094..3210)
                     /locus_tag="AABJ92_RS24830"
                     /old_locus_tag="VEE38_47950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /name="IS1595 family transposase"
     gene            complement(4029..4340)
                     /locus_tag="AABJ92_RS24835"
                     /old_locus_tag="VEE38_47960"
     CDS             complement(4029..4340)
                     /locus_tag="AABJ92_RS24835"
                     /old_locus_tag="VEE38_47960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517562.1"
                     /translation="MAKAAERLAKLEEQRARINAEIQRVKAREQQQKRKEETRRKVLVG
                     AWMMGKVQSGEWPEQKLIEAMDSYLERDHDRALFGLPPIQPKPQQQPEHVQYSQEVQG"
                     /name="hypothetical protein"
     gene            4511..>5152
                     /locus_tag="AABJ92_RS24840"
                     /pseudo=""
     CDS             4511..>5152
                     /locus_tag="AABJ92_RS24840"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            5259..5669
                     /locus_tag="AABJ92_RS24845"
     CDS             5259..5669
                     /locus_tag="AABJ92_RS24845"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_225883815.1"
                     /translation="MSAIDRVRKSRGINELAAEIEPLAQSMATLADEARQRIAEVQQAS
                     EEQAASWTSQQQQAMSAWREAAKDMRTAAGELTTASQTARSAARGWTWRLWVGVLIASV
                     TPILVLLIASWLWLEPQVIEQQGGIWLIFKLR"
                     /name="IncQ-type mobilization protein MobB"
     gene            <5768..6607
                     /locus_tag="AABJ92_RS24850"
                     /pseudo=""
     CDS             <5768..6607
                     /locus_tag="AABJ92_RS24850"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /name="DNA-primase RepB domain-containing protein"
     gene            6667..6921
                     /locus_tag="AABJ92_RS24855"
                     /old_locus_tag="VEE38_47980"
     CDS             6667..6921
                     /locus_tag="AABJ92_RS24855"
                     /old_locus_tag="VEE38_47980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517558.1"
                     /translation="MKIEIELHDSVAAELAYIVELHKEHGAANAQDSVEALLAYVASAI
                     AAGSRRPGAWERTCLDMMGLVANTDEHHYYRSGYGRPEA"
                     /name="hypothetical protein"
     Typing          4375..4461
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.318"
                     /coverage="98.0"
                     /evalue="5.47e-30"
     Typing          2..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.929"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4512..6604
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.469"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat cgacattctg gcgagtttca cagagctacc gccaccgctg
       61 gactacgttt tgcccaacat ggtcgccggt acggtcgggg cgctggtatc gcctggcggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcgggcgg gccggatctg
      181 ctggaggtgg gcgagctgcc gacaggccga gtgatctacc tgcccgcaga agacccacca
      241 gttgccatcc atcaccgcct gcacgccctt ggggcgcacc tcagtgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca acctgcctaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgagcc gctgagggac gacggttgat ggtgctggat
      421 accttgcgcc ggttccacat cgaggaagag aacgccagcg gccccatggc gcaagtgatt
      481 ggccgcatgg aggccatctc cgccgatacc ggctgttcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggatcgtcc
      601 gtgctggtgg ataacatccg ctggcagtcc tacctgtccg gcatgaccac cggcgaggct
      661 gaggaatggg gcgtagacga cacacagcgg ccctatttcg tccgctttgg tgtcagcaag
      721 gccaactatg gagcaccgtt caaagaccgc tggttcaggc ggtatgacgg cggagtgctc
      781 aagcccgccg tgctggaaaa gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgcagc ctcaagcgag gcgagcgcaa gcgcggcaag ctggacgtgg tgtatgacta
      961 cggcgacggt aagcgcatcg agttcagcgg cccggagccg ctgggcgctg atgacttgcg
     1021 catcctgcaa ggacttgtgg caatggctgg gccgggtggc cttgtgctgt cgcctgagcc
     1081 gcagaccgag gccgggcagc aactccggct gttccttgag ccgaaatggg aagccgtcca
     1141 gcaggatgcg atggtggtca agggaagcta tcgagcactt gcccgcgaaa ttggctacgc
     1201 caaccccgac gacactaagc cggttcggga gtgcatcgag cggctttgga aggtgtccat
     1261 catcgcccag aacggcagga agcggcaggg cttccgcctg ctgtccgagt acgccagcga
     1321 cgagcaggac ggaaagctgt atgtggcctt gaatcccctg attgcttgcg ccgtcatggg
     1381 tggcgcgcag catgtccgta tcgacatgga cgaggtgcgg gcgctacaga ccgacccggc
     1441 gcggctgatg catcagcgcc tatgcggctg gatcgacccc ggcaagtccg ggcgggtgga
     1501 actggacacg ctttgcggct acgtctggcc ggggcaggca accaacccca atacgctcaa
     1561 gacgcgacgc caagcggctc gcagggcgct tcctgagctt gccgcattgg gctggacagt
     1621 cagcgaatac gcaaaaaaca agtttgaaat cggcaggccg aaagcaacgc atctgctcgg
     1681 cccagcaccc aaaacacagg aaattagcca tacagcctga gggaggattt aggaagcacc
     1741 gcaccctact gaatggcatg gacttctttg agtttactca aaaatggagg gagttttgta
     1801 tttacaacag cacccgtcag gttcctaacc ataaagaaac aaagtataat aaacacacat
     1861 gctactaatt aaaataaata aaggtaatta attttgaaga taaattataa attgacaatt
     1921 gctaacgcta ctgttctggt ttgcttactc ccttacatat caccatttaa aactggactc
     1981 gacagccagc catacggtct tatatttatt attatatcat ggttaactct ttataaacat
     2041 caaactaagc acaaacactt catgctatta tctttaataa cattcctttt atccagtata
     2101 attttaactg tagaagccat tatttatggt gatactttat cttttattag gggagtgtat
     2161 gggtattttg ttttatttgt agctcctttt tatttcttaa cctaccttag gttttacgac
     2221 ttcccaaaga ggacatttta tttatcatca tcattgtatt tcattgcagc aataattcaa
     2281 gctttcgtag atgtgaattt ttttgatttt ttggttgaca ttagaacaag cgaatctaga
     2341 ggtgtaacat ctcttacgcc agagcctact tattacggta ttgtttgctt attacttctt
     2401 atggtatgcc tagtatctga atcctttaaa aataaagaaa ggaaatatct ctcgttgttt
     2461 tttgttatac aaatagtatt tcttgcaaaa tcatcaatgg ccatactctt gttgttgatt
     2521 ttatcttttt atttcctgtg cttaaaaaag attagttcga caaaaattct tttaggctta
     2581 tttttacttc tttgtggtca ttttgggatt ttacacctaa tggaaggtag cagagtttat
     2641 gacttataca caaggtcaat tgaattggga ttttatttat tcaagattga tgcaagcatg
     2701 aatgaacgac tgtcgcatat ttatttctct ttatatggaa tgttaaataa tgctttttta
     2761 ccaaacttta atagtaactt ttctacttta ctagataata ggtacatttt atctgatggt
     2821 tttttttggt atggagaatc cggtaaaaaa atcatgagct actggggcgg ggtgtttttt
     2881 aatctaggtt tcattggtgt attttattat ataattcttg cttcgagtgt aaaaaaagga
     2941 cagctaggtg ttttcctttt cgttttaaca ccaatattat ttacagcagt tcctatggct
     3001 atgacaatcg taagtttttt attttccatt tttattttca gatcagaatt aaggaaacct
     3061 tgctaaattt ctgcgtgcat agctaacacc caagccttgg ggataacgcc acttaaagag
     3121 agctttctcg cacagctctt ccgtgccgta cttctccaaa aaatcgttta cgctcagacc
     3181 cttctgatac tgaactttgt tcttggccat ggctgcaccc tttgcttgat ggctgtatac
     3241 gcatacagta taggcatggc tgcacaacat gggtaatcag gaaaaatttt gaacggaatg
     3301 gtaacaaatg agcctcatga tgaaaggtga agatggcaga aatcagtgcc ggatcaacaa
     3361 acagataatc aaatagagaa cgagcggcct tctaatgagc cggaaaccgg cgagcactgg
     3421 ggcagcgtga tctggtgcga gtatgtgtga cccccacggt aactccaccc aacccccacg
     3481 gtaactccac ccaaccccca cggtaactcc acccaacccc cacggtaact ccacccgccc
     3541 caatctggaa aacccagacg gggcgcggat ttgctggcga ttcgcaaaat ccatccaaga
     3601 ttctccaaga atctccacta ggcgcagcac agcccgcgcc ccttcggcgc gggctggcat
     3661 tgcccaaaat gcccggccag aggccggata actgatctat ccgctgcgct aggctacaca
     3721 ccccctccac cccaccgcta cgcggtaggg tgaaaggcgg gctttgcccg ctggtgccag
     3781 atcaagggca gtggttggct caggggctgt tttgccgctt tagcggcctt tacccctacc
     3841 cgaagggtgg gggcggtgtg tagccccgca gggaggcgtt ttaggggctt ctgcgcagtc
     3901 cgtgcaaagc aacttcctct ctgccctgct tcgcgggtcg agggcatgag ggccacagtt
     3961 catcgtgcag acctttgtat ccgggtcgaa tgccgccagt tctgccgggt cagcgcggcg
     4021 ccagtcactc acccttgcac ctcttggctg tactggacgt gttctggttg ctgctggggt
     4081 tttggctgta tgggcgggag tccgaacagt gcccggtcgt ggtcgcgttc cagatagcta
     4141 tccatggcct ctatcagctt ttgttcaggc cattcaccac tctgcacttt acccatcatc
     4201 cacgcgccga ccagcacttt tcgtcgggtt tcctccttgc gcttctgctg ttgctcccgt
     4261 gccttgaccc gctgtatctc ggcgttgatc cgtgcccgtt gttcttccag ctttgcgagc
     4321 cgttccgctg cttttgccat gttgacagtc cccttggtta tgcgcttgtc aacatgctat
     4381 catcggagca cagatgccgc aaacccatac cgtcattctg acgggatggc gcacttaccg
     4441 gtttctcttc aagaaactgc ccttcggggc tgtacgctct ccgagggcca ttgcatggag
     4501 ctaaacccct atggccatct atcaccttac ggccaagacc ggcagccgga acggcggcca
     4561 gtcggccaag gcgaaagccg attacatcca gcgggagggc cgttactccc gcgaccgtga
     4621 cgaggtatta catacccagt ccggccacct gcccaagtgg gccgagcgtc ccgccgacta
     4681 ctgggacgtt gccgacctgt acgagcgggc caacggtcgc ctattcaaag aagttgaatt
     4741 cgccctgccc gtcgagctga ccctagacca acagcgtgag ctggtggacg aattcgcccg
     4801 gcatctgacc gagggcgagc gcctgccgta cacgctggcc atccatgccg gtgacggcga
     4861 gaacccccat tgccacctga tgatctccga gcgcaagaac gacggtatcg agcgcccggc
     4921 tgaccagtgg ttcaagcggt acaacgccaa acagccagag cggggcgggg cgcagaagtc
     4981 cgaaagcctg aagcccaagg catggcttga gcagacccgc gaggcatgga gccaccacgc
     5041 caaccgcgcc cttgaacggg ccgggcacga ggcccgcatt gaccaccgca cccttgaggc
     5101 gcagggcatc gagcgcctgc cgggcatcca ccttggcccc aacgtggtgg agatggaggg
     5161 ccggggcatc cgcaccgagc gggccgacac agccctatcc atcgacacgg ccaacggcca
     5221 gatcatcgac ctacaagaat accgggaggc catcgagcat gagcgcgata gacagagtga
     5281 ggaaatccag agggatcaac gagttagcgg cagagataga gccgttggcc cagagcatgg
     5341 cgacactggc ggacgaagcc cggcagcgga tcgccgaggt acagcaggcc agcgaggagc
     5401 aggccgcgag ctggacgagc cagcaacagc aagccatgag cgcatggcgg gaggcagcca
     5461 aggacatgag gacagccgcc ggggagctga ccacggccag ccagacggcc cggagcgccg
     5521 cccgtggctg gacgtggcgg ctatgggtag gagtcttgat cgcttccgtg acgcctattc
     5581 tggtgctgct gatcgcatca tggctttggc tggagccgca agtaatcgag caacaggggg
     5641 gcatatggct gattttcaag ctcaggtgaa aggtgatcgc accgcgcagg ccatcgctag
     5701 acagctcaag gccatgggct gtgaccggta cgacattggc atacgggacg ccgccagcgg
     5761 caagatgatg aaccgggaat ggacaccgca ggaagtgcag cagaacgccg actggctcaa
     5821 gcgcatgaat gcccagggca atgatattta catccgccct gccgagcagg cccggcatgg
     5881 tcttgtgctg gtggatgacc tcagcagcga cgatctggac gccatgaagc aggagggccg
     5941 ggagcctgcc gccatcatcg agaccagccc gaagaactat caggcatggg tgaaggtggc
     6001 gcaggacgcc ccagcagttc acaggggcgt gatcgctcgg gagctggccc gcgagtatga
     6061 cgctgacccg gcaagtgctg acagccgcca ttatggacgc ctagcgggct ttaccaaccg
     6121 gaaggacaaa cacaccagca acggctatca gccatgggtg ctgtgccggg agtccagcgg
     6181 ccagatcgcc accgcagggc cggagctgat gcagcaggcc gggcaggtgc tggacagcat
     6241 caagcgccga caggagaagg cccgcaggct ggaaggcatc gaggccgggc caaaacgcag
     6301 ctatcgccgt gacgccgtgg acgactaccg cagcgaaatg gccgggctga tcaagcggta
     6361 cggtgacgac ctcagtaggt gcgatttcat cgccgctatg aagttggcaa gcaatggccg
     6421 ggagccggac gaaattgcca aggccatggc cgaggccagt cctgcaatca tggatcgtaa
     6481 ggcgggccat gaagctgatt acattcagcg cacgttgcag aaggtgatgg agctgccgca
     6541 agtgcaggag gcccgcgccg agctagcgag gcaggcgcaa agaaaagggc ctgaacctgg
     6601 tatgtaggtg ttgcattagc gctaatgttt gtcatgatgt tagctctaac ggaaaagggg
     6661 cgggttatga aaatcgaaat agaactacat gacagcgtag cggcagagtt ggcttacatt
     6721 gtggagcttc acaaagaaca cggcgcagct aacgcacagg atagcgttga ggcgctactg
     6781 gcctatgtgg catcagccat agcggctggc agccggaggc cgggggcgtg ggagcgtact
     6841 tgtcttgata tgatgggatt ggttgccaac accgacgagc atcactacta ccgctctggt
     6901 tatggaagac ctgaggcatg aaagaccaac acacccctaa cgctatacga caggcccgct
     6961 ttgccgagca gcagcgagcc atgggccgta aggcacggaa gatttgggcc acagacgcag
     7021 aggccgaggc gctgcgtctg tacctagagg aacttagggc ggcgcaggac ggggagaacg
     7081 gccccgccag cgcctgacca tcaaccgcct acacaggagg caaatca
//
