LOCUS       NZ_AP027629            12049 bp    DNA     circular CON 04-APR-2024
DEFINITION  Escherichia coli strain PSJ-14 plasmid pPSJ-14_3, complete sequence.
ACCESSION   NZ_AP027629
VERSION     NZ_AP027629.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00562563
            Assembly: GCF_036499205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hoang,H.T.T., Yamamoto,M., Calvopina,M., Bastidas-Caldes,C.,
            Khong,D.T., Nguyen,T.N. and Yamamoto,Y.
  TITLE     Comparative genome analysis of colistin-resistant Escherichia coli
            strains possessing mcr isolated in Ecuador and Vietnam
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12049)
  AUTHORS   Yamamoto,Y. and Hoang,H.T.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2023) Contact:Yoshimasa Yamamoto The United
            Graduate School of Drug Discovery and Medical Information Sciences,
            Gifu University; Yanagido 1-1, Gifu 501-1194, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Coverage                   :: 232x
            Sequencing Technology :: MGI DNBSEQ-G400RS; ONT MinION Mk1c
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036499205.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 00:04:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,972
            CDSs (total)                      :: 4,854
            Genes (coding)                    :: 4,610
            CDSs (with protein)               :: 4,610
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 244
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 72 of 244
            Pseudo Genes (incomplete)         :: 155 of 244
            Pseudo Genes (internal stop)      :: 57 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            Pseudo Genes (short protein)      :: 3 of 244
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP027629.1.
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="PSJ-14"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pPSJ-14_3"
                     /geo_loc_name="Ecuador:Puyo"
                     /collection_date="2019"
     gene            join(11409..12049,1..19)
                     /locus_tag="AABJ92_RS24735"
     CDS             join(11409..12049,1..19)
                     /locus_tag="AABJ92_RS24735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_217300911.1"
                     /translation="MTEIKKESADDAWLRSTLAELAGDAFPVYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAEADGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            202..591
                     /locus_tag="AABJ92_RS24740"
                     /old_locus_tag="VEE38_47780"
     CDS             202..591
                     /locus_tag="AABJ92_RS24740"
                     /old_locus_tag="VEE38_47780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149015768.1"
                     /translation="MNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAPIG
                     APVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAPAF
                     AEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            584..946
                     /locus_tag="AABJ92_RS24745"
                     /old_locus_tag="VEE38_47790"
     CDS             584..946
                     /locus_tag="AABJ92_RS24745"
                     /old_locus_tag="VEE38_47790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186829.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
     gene            939..1124
                     /locus_tag="AABJ92_RS24750"
     CDS             939..1124
                     /locus_tag="AABJ92_RS24750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162780107.1"
                     /translation="MSEFAVSRLVIPEVVIPDGVIAARRARGQAQRVAAARRAAERRAA
                     PTWRWVRREQPRSTGR"
                     /name="hypothetical protein"
     gene            complement(1144..1785)
                     /locus_tag="AABJ92_RS24755"
                     /old_locus_tag="VEE38_47800"
     CDS             complement(1144..1785)
                     /locus_tag="AABJ92_RS24755"
                     /old_locus_tag="VEE38_47800"
                     /inference="COORDINATES: protein motif:HMM:NF016255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_112918740.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1782..3071)
                     /locus_tag="AABJ92_RS24760"
                     /old_locus_tag="VEE38_47810"
     CDS             complement(1782..3071)
                     /locus_tag="AABJ92_RS24760"
                     /old_locus_tag="VEE38_47810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062810.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFTQNETRSLAKIPV
                     ILPQREQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLTLSVLAAVGRWDYINDI
                     IRRIDRDRGDAATLIALADHIQTNRPERDL"
                     /name="hypothetical protein"
     gene            3425..3634
                     /locus_tag="AABJ92_RS24765"
     CDS             3425..3634
                     /locus_tag="AABJ92_RS24765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_148502023.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPMLKRFIADQRRPGRATDGVR"
                     /name="hypothetical protein"
     gene            3621..4787
                     /locus_tag="AABJ92_RS24770"
                     /old_locus_tag="VEE38_47820"
     CDS             3621..4787
                     /locus_tag="AABJ92_RS24770"
                     /old_locus_tag="VEE38_47820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174599769.1"
                     /translation="MAFDNSHLADPGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSARHNRGEAKHRSQYRHFLKLKGITNPEAMAQVFLQFSGVEWNRQASVQ
                     SDADRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALAAA
                     GGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCSAE
                     GFQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLSRG
                     KVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPVPTIPG
                     AAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERG"
                     /name="hypothetical protein"
     gene            5044..5502
                     /locus_tag="AABJ92_RS24775"
                     /old_locus_tag="VEE38_47830"
     CDS             5044..5502
                     /locus_tag="AABJ92_RS24775"
                     /old_locus_tag="VEE38_47830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_174599770.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPRQ
                     QTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     IVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLPWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(5520..6008)
                     /locus_tag="AABJ92_RS24780"
                     /old_locus_tag="VEE38_47840"
     CDS             complement(5520..6008)
                     /locus_tag="AABJ92_RS24780"
                     /old_locus_tag="VEE38_47840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062818.1"
                     /translation="MREKTRTLTVRLPVSLIERIEQRAEKTKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKDARQSAPQTARQSALQNARQIVPRGADPRFQ
                     AVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYRHKLRERGLI"
                     /name="hypothetical protein"
     gene            6519..8090
                     /locus_tag="AABJ92_RS24785"
                     /old_locus_tag="VEE38_47850"
     CDS             6519..8090
                     /locus_tag="AABJ92_RS24785"
                     /old_locus_tag="VEE38_47850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062821.1"
                     /translation="MSNHEENSGAAADNVTPITAAPSPLSLKLGDALFSVLSVSADWSG
                     DYRAQFELYGLDVKAINSAVGTAVWHAGKGRFLSVLNGSLTEFDKGDGMKLLEDSCGKF
                     WHRTDAFIARLTDLKIKTDDKVTKACIDMARAVRQAVAEFIMLRRQVAVVRLDVDMFAT
                     APRVELVGETVTFVRPHAPYPVANADSDVVADWLVHFPQCHEFLDALVAARFASSRKNA
                     YLFFRAQSDWGKGLLFGAGGVLSRLGATVELSEGELLNILSGANSGVTASHFMGALALI
                     VNECTRVTKKHFRLEESLALTPKYLTTQCVNLYMKIFTSADPIPGLSDSDGIDPQVANR
                     FSMLDLQGDIKTRPLFLSDKGRYVDSLTSFFAAELNARVASYQAKGFETARRDADHVLA
                     AFTTAHGLANAAGLITDAYDEIRAEWVAFVHGRVADGHPDFLTFESKAGLVLRSPVGCW
                     GKFLDWYVDKSEPLRRARLMHDRDLIIGCAKKYRDVGGAAEGKGVLIPFVSGSMPDNVH
                     RFRYKN"
                     /name="hypothetical protein"
     gene            complement(8711..9178)
                     /locus_tag="AABJ92_RS24790"
                     /old_locus_tag="VEE38_47860"
     CDS             complement(8711..9178)
                     /locus_tag="AABJ92_RS24790"
                     /old_locus_tag="VEE38_47860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064186840.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9376..9618)
                     /locus_tag="AABJ92_RS24795"
                     /old_locus_tag="VEE38_47870"
     CDS             complement(9376..9618)
                     /locus_tag="AABJ92_RS24795"
                     /old_locus_tag="VEE38_47870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_316279503.1"
                     /translation="MAFENIERRFQRWKVIMVPGGKSYESWDEMVTGENFPEEARYKYK
                     THRTKAIDLTLKNPDYVHTMSENGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(9638..9988)
                     /locus_tag="AABJ92_RS24800"
                     /old_locus_tag="VEE38_47880"
     CDS             complement(9638..9988)
                     /locus_tag="AABJ92_RS24800"
                     /old_locus_tag="VEE38_47880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104691675.1"
                     /translation="MRTKAIIAAALLATAGTAAAAPVATTYICNDGTFIHLNEQNGRLT
                     ASWRGADIPVTKIETGKLTASLNGEQGSSTLQLVVGYNTTPNPIAIITTVVNGSMNSQS
                     CKPDTASKHIDL"
                     /name="hypothetical protein"
     gene            complement(10089..10280)
                     /locus_tag="AABJ92_RS24805"
                     /old_locus_tag="VEE38_47890"
     CDS             complement(10089..10280)
                     /locus_tag="AABJ92_RS24805"
                     /old_locus_tag="VEE38_47890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060634173.1"
                     /translation="MTPGRTQILKATADIAKALGTDTADVERHFLQHAVVLARLRQEGK
                     RPRVKAWMAENIGTPSAM"
                     /name="hypothetical protein"
     gene            complement(10583..11158)
                     /locus_tag="AABJ92_RS24810"
                     /old_locus_tag="VEE38_47910"
     CDS             complement(10583..11158)
                     /locus_tag="AABJ92_RS24810"
                     /old_locus_tag="VEE38_47910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151785.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_101998997.1"
                     /translation="MSRVFGYGRVSTGEQTVAGQKKAILDRYPQATFHADEGISGTVPA
                     TQRDKFKAILEFAAPGDSIVVVAIDRIGRNARSVMEVCEELEKRQVSLISLREGFDFST
                     PTGKMMLTMVAAFAEMELATLKERTKAGMAAAAAAGRHAGRPTKQTPENVGAVIMKHDA
                     GVSAARIARDLGISRGTVNRILQKHAKA"
                     /name="recombinase family protein"
ORIGIN
        1 tggcatggag ggtgtgtaat gtctgctttc aaactcctgt gtgaccggct tcgcaatgag
       61 gcgacgctgg caaaatctga cggggccggc gctttcaaaa agcaatctct ggatcccgac
      121 ctgaagggtg gcaagaaggg caaaaagccc gctggggagc tggaggagga cgaggcgatc
      181 gcgtctctcc accgtaagcg catgaacgac gttaaaggtt gcaagacgct gaccaaatcc
      241 accggcgctg atgactttgg cctccctccg ctgtcgtcgg tggtcgagac agtggtggag
      301 acaggcggcg cagcgcagac gttcgcgccg atcggggctc cggtggcgtc tccgaccgaa
      361 gcggttcacc gtgcgcgtgc gcagtccatc caggacgcgg cgttcctgaa cgagatgaaa
      421 gcggcgggtg tggcaccggc tgacctggtg ttacttgacg gcgtgttgac gtcctctcgt
      481 gggatgccag acgcggcgcg tgctccggcg tttgccgagg tgcgtcgtca ggttcaggcg
      541 ggtggcatga cgctgtcacc ggctgcgcgt ggtattgact ggaatgcgta agctgccgcg
      601 tcacctgcag ggcgaggtgt tgatgccgct gtccagcagg atcacgcgct cgcaacggct
      661 ggagctgatc tggctgttgc ccgttctgcg gctccacccg ggcgatcgat cggtcactga
      721 gcggcgggcg cttggcaggt tccggggatc gctcgctgcg ggtggccaga cggatgcttt
      781 cgtccgttcg gatgcgtctc tgtacgcgct gggactgcgg ttccagcgca agcgcacccg
      841 cgagctggtt gcttcggatc ccgatgttgc tgcggctggc gaggcgctgg agcagcgtgc
      901 aaacggcgtg aacctgtccg agacgttcgg gggtggcgat gagtgaattt gctgtctcgc
      961 gcctggtcat tcctgaggtg gtgatccccg acggggtcat tgctgcccga cgggcaaggg
     1021 gacaggctca acgtgtcgca gctgctcgcc gtgctgcaga gcgtcgtgct gcgcctacgt
     1081 ggcgctgggt gagacgggaa cagccccggt cgacagggcg gtgaaacggt gagacggggc
     1141 gcgttatgcg cccttttttt cgcccttcgc ctggcgtgcc ttgcgccgga atgccttgat
     1201 ctctgtcagg cgggctttgg cgttttcggc ctcctccggg gttgctggtt ctgtggggtt
     1261 gccgtccagg tcgtagcgca tgcactggtc gatgaggctc tgctggtacg agctggtacc
     1321 cgtgacaatg cccaggatgc gacggacggc tttgcggtct gcctctggcg gcatgtccag
     1381 ctctgcgcct cgtgtcagga tctgctcgat gatgcccttt gccatggggc gacggtcaac
     1441 tccccgggtc agtaagtcag gccagtgctc tttcaggaat gctttggtca cccggttggc
     1501 cagcgccttt ttggacttgt tggcgatcgc gatgtcctgc tggcggcggt cgttctcctc
     1561 ctgtctgcgg atcccctcgg cgacctgctc gggcgtcttt ttcggctttg ccgctgtggg
     1621 cttctgtgcg ggcttctgcg gggctttcgc ttttggctgt accttcgcct tgctggtagt
     1681 ccgttcggct ggctgtggct cagatttcgc ggccttttcc ggcgggacgt agaccgtgcg
     1741 gttgcgtcgc ttgatggtca ggatcgggcg ggtgggttcg gtcataaatc tctctcaggt
     1801 ctgttggtct ggatgtggtc ggcaagggcg atcagggtgg cggcgtcgcc gcggtcccga
     1861 tcgatgcgcc ggatgatgtc gttgatgtag tcccagcgcc cgacggcggc aaggacggaa
     1921 agggtgaggc ggttgaaccg actctctggt acccggtcga gggtcagggg gatgcgcttg
     1981 cgggggcgtc ctggaccgcg ccctgtccct ggtcttttga gcagctgcgg ctgctccctc
     2041 tgcggcaaaa taacggggat cttcgccagg ctgcgcgtct cgttctgcgt gaactcgggc
     2101 accgcgatgt gctcgtctgt ccatgtcctg cctccgggcc tggctgatgc gctggagtcg
     2161 caggacgggc aggagacgac gatctcccgt cgcggcttgc ccttgaccgg tgccttgctg
     2221 atcaccaggg gcgcggcttt gcaatcggag cagaccgacg ctgtccagac cgcgttgtct
     2281 ggcagggtgg cgttccggta ctggtgcgcg gcgtcatgcg gtgtctggaa gctgaccgtc
     2341 tgaaacgtgg gggcgacgct gaggggtgcc gcccatggct ggcacctggg acaagcaagc
     2401 tccacctctc cggcgtgccc gtcgccaccg ttccagcgca ggggctcgcg gtgttcgtgg
     2461 cagtagtaca cgaaaccgtc gcggcgtgtt tgcccgaccg ggcctggtcg gcgatcgtgg
     2521 aaccgtgcgc ccgggttggc tcgcatggcg cggacgagcg cctcgccctg gtggatgccg
     2581 taccagacct gccagccatc gccgatgccg atcaggaacg gcgagacgaa cggtggggtg
     2641 cggttgactc tcccgcgtgc gttggctccg atggtggctt cgcgttcgcg gttccagcag
     2701 gagacgcaca ggctcgaacc gggcttattg ggggcgatca ggcggttgtc tttccggctg
     2761 caacgggggc agagcttacg ggtgtctgcc ggggtggtca gtggtgcgtc agggggctgt
     2821 tcggtaccga cccaaagcgc gaggccagtg gggcaccggt ggcactcgag gtgggggttt
     2881 tcaatcgtgc gatcgaggtg gctctgctca taaatctgtc ggcatgcctc gacccggagg
     2941 gagcacccgc cggggcaggt gacgaacaaa acgccgggga gctgctcggg gaatttttca
     3001 gtcgcgctta cgcgttcaag tgctgccggg ttcggctgcg tcgattcgtt cgtgtcgtta
     3061 tcgtcgttca ctgctccccc tgcgtcgaaa tggtcgccag acagcgccag cactggctgt
     3121 gcgtcttttt tagtatctca caaaaaacgc cactttggcg ttaattgtgt tacgcttgtc
     3181 ttactgattc ctgacgagca gttgggaaac atgaaaccgg atcgcccggt gccgctttcc
     3241 ctcctcccca acgaggcggg gttgtaaacg aggatgcgcg cttcgtcctt ggcaggtgcg
     3301 agtaagcgag aagggctgct gtcctgccgc tatgcggtaa gtcctcaagg gcggctttat
     3361 cgacaatcgt cactcctgcc cccgatgccc gttaagctga gggggcacct atctggtaca
     3421 cccaatgaaa aaacgaatct ataccgctga cctcgccgca ctattgaatg tgtcgctgcg
     3481 tacgctctcg cggttaagtt cgcgggggat cgtgccgact ccggcagggc gctccctccg
     3541 tggcgggcat tactggctgg ctggcgatcc catgctcaaa cgctttattg ccgaccagcg
     3601 tcgtccgggg agggctactg atggcgttcg ataactctca cctggctgac ccgggtctat
     3661 tctggcgtgc cgtgtcggct gcgggtgctc cggttgaccg tgaggactgg ctgcgcctct
     3721 gttccggcat agcgtgctgg gcatctctct gcggcgagga tgggctggca gctatcgagg
     3781 cctggtctgc ccgccacaat cgcggggagg caaaacaccg gagccagtac cggcatttcc
     3841 tgaagctgaa aggcataacc aatcccgagg ctatggcgca ggtgttcctg cagttctccg
     3901 gcgtggagtg gaaccgacag gcttctgttc agtccgacgc cgatcgcgct gaacggctgc
     3961 ggttgtcccg tgaggccaca gcgaaggcaa aggctgaacg cgacaagaag ggtgatgctg
     4021 ctgcgcgtgc tgcggtcaag ctgctggcga agggagggga tcctgcgtct accccatact
     4081 ggcgcaaaaa gaccgctctg gcggctgcgg gtggcgtcca gctgcccgcc ctcagcacag
     4141 atcttggtgt tggcgggatg tttcgatcgt tcaatccgcg tgcttactgg tctgattgcc
     4201 tcctgattcc ggtttacaag ttcgacgccg cccgtcagct gtatgttgac ggcctggagg
     4261 ccatctgcgg gcgttgctcg gctgagggtt tccagctcac tggctcgaag ttctctccgg
     4321 cgggtacccg caagcagggc ggcttctacc cgatgccggg gttcatggac gtgctcgacg
     4381 gctccccgca cgcgccctac tcgcctctgt tcgtctgtga ggggttcgct acggcgttcg
     4441 ccctgcacca cctgagccgt ggcaaggtgg cggtctgcgc tgcgttctcg atcgccgggt
     4501 tcggcagctg cgtggcgtcg ctgcgtcggt tctggccaga tgttgatgtg atgctggtac
     4561 cggatcacgg cgaagcgtac cgcctggcgc ataaagcggc ctatggcgat gagcgagagc
     4621 cggtcccgac aattccaggc gcggcggttg tgtcgttcga cggcctcgat cggttcactc
     4681 cccgggataa cctcgactgg ttcgacgtgc tggttgagga gggcgaggat cgggcgcgtg
     4741 agctgctccg gtatgcggtg cgtcatgccc ggcttgagcg gggctgatgg ctgggctttt
     4801 ttgcgtcggt ttcctcggga ggccgctgac cgtaaagggt cgttttctgg ctcgtctttt
     4861 ttgcagtcgc ttttgggttg gtgagacaac ctttaagacg cacatccgac cgcttccggt
     4921 ttcctccgtt ttagacgccg ctgcggtcgt gacgctatcc tgcagctggt ttggtttcca
     4981 gcagcacact caagcccggc accgttttgg tgtcgggctt tttttcgccc gtaaggaggg
     5041 cacatgaaat caaagactga attacgcgct gccgcgacgg ctcgcgccct tgaggtgatc
     5101 gcttctgaga tggggggttg gtcgctcgac gggtttgctc acggctcgct gccgaacttc
     5161 tccccgctgc cgcgccagca gacgcaggag ggctctgtgg tgctcgaacg cccgccgttc
     5221 gactacacct gggcgggtac cgctgcgttt accgatcgtg caaaccgtgc gctgcaggtg
     5281 aagctaccgg cgtcgcgtga gcgtaactac atctggctgt gcgcggtcga aagggaggct
     5341 gtggccaccg cgctgatagt ggagtcgttc aacgtgaccg gctgcgcggc gttcgccggg
     5401 ttgcctcctg tggatggcat ggtgctgctg accatggacg aggccgacgt ggagctgatc
     5461 agggcggcga tgctgccgtg gctggatgcg gcggcggcgt gagccgtcgc ccctctgcgt
     5521 caaatcaggc cgcgctcgcg cagcttgtgg cggtagtccc ggacgcggct ggtattccac
     5581 tcctgcccgg tggcggtggt gtgcccggct gcgttcagcg cctgggcgat cgcgggcagg
     5641 gtggcgttct ccccggtcat gctgacgatg agctggtcgg cctcatcgct gacggcctgg
     5701 aagcggggat cggctccccg ggggactatc tgtcgtgcgt tttgtagtgc gctttgtcgt
     5761 gctgtttgtg gtgcagactg tcgtgcatct ttcccgcctc cggccagcgc cctgacctct
     5821 gaccgcagcg cctcgatggc ggtcaaaagt ttggtgtcca tcccttccgg cttcgtgccg
     5881 agtccggcga caagcgcctc tatgacggca tctgtgcggg ttactttggt tttctccgct
     5941 cgctgctcga tgcgctcgat caggctgacg ggcagtcgaa cggttagggt tctggttttc
     6001 tctcgcatgg gctggcctct ccgtgtcgtg cgttttgtcg tgctgtttgt cttgcggagt
     6061 gtaatgcgtt ttgtcgtgcg cgtcgaattt gacgtagtgc aattgtcgtg cagggagggg
     6121 tagcggctcg gcccgtggat aacttctgtg gataacaaaa tttcgaacgt tccggcccgt
     6181 ggataaatcg cggaggctgc gcggctctaa gatcggtgcg ggatcgcgtt tcgcgtggtt
     6241 ggatcgttgt gtactggtgc aggggggtat gtaacggtat gatggcgcgg ctgaaaaaga
     6301 aaacccgtcc gggcagacgg gtcgttttct taaagctgtt ttgagtttgg agctttcgcg
     6361 gcggtcaccg tggaagacgc cataataact catgttgggt gtgactccag catgaattat
     6421 acgcgatcat aatgacgcgt gcaaagggtg ttttccccgt tatcccccgc cgttgtaagg
     6481 ctcctcgaga ttggctgttc aattttgagg atgtaaaagt gtcaaatcat gaagaaaatt
     6541 ccggcgctgc tgccgacaac gtaacgccga tcaccgctgc accgtcaccg ctgtcgctaa
     6601 aattgggcga tgcgctgttt tctgtcttgt cggtgtctgc ggactggtct ggtgactacc
     6661 gcgctcaatt cgagctgtat ggcctggatg taaaagctat caactctgcc gtcggcactg
     6721 ccgtctggca cgctggcaag gggcggttcc tctctgtcct aaatggctcc ctgaccgagt
     6781 tcgataaggg cgacggaatg aagctgctgg aggactcctg cggcaagttc tggcaccgca
     6841 ccgatgcgtt tatcgcccgc ctgactgacc tcaaaatcaa gaccgacgac aaggtaacca
     6901 aggcgtgtat cgatatggct cgggctgttc gtcaggcagt ggctgagttc atcatgctgc
     6961 gccgtcaggt ggcagtcgtt cgcctcgacg tggacatgtt cgccacggct cctcgcgtcg
     7021 aactggtggg tgaaactgtg acgttcgtcc gtcctcatgc tccctatccg gtggctaatg
     7081 ccgacagtga cgtggtggct gattggctgg ttcatttccc tcaatgccat gagttcctcg
     7141 acgcgctggt tgcagcccgc tttgcctcaa gccgtaagaa tgcctacctg tttttccgtg
     7201 cgcagtccga ttggggtaag gggctgctgt ttggtgctgg cggcgttctg tcacgcctgg
     7261 gggcgacggt ggagctgtcc gagggggagc tgctcaacat cctgagcggt gccaactccg
     7321 gtgtaacggc tagtcacttc atgggggcgc tggcgctgat cgtcaacgaa tgcaccaggg
     7381 tgacgaaaaa gcactttcgt cttgaggagt cgctggctct gacgccgaag tacctcacga
     7441 cgcagtgcgt gaacctgtac atgaaaattt tcaccagtgc cgacccgatc cccggactgt
     7501 ccgactccga cggaattgac ccgcaggtgg cgaaccgctt ttctatgctc gatctgcagg
     7561 gcgacatcaa gacgcgcccg ctgttcctgt ccgacaaggg acgctatgtc gacagcctga
     7621 cgtcgttctt tgccgccgag ctgaatgccc gggtggcgtc ctatcaggct aagggctttg
     7681 agaccgctcg ccgcgatgcc gaccatgttc tggctgcttt caccactgcg catgggttgg
     7741 cgaatgctgc cgggctgatc accgacgcgt atgacgaaat tcgggctgag tgggtggcgt
     7801 tcgtccatgg gcgtgttgct gatgggcacc cggacttcct caccttcgaa tcaaaggctg
     7861 gtttggttct ccgctctcct gtcggatgct ggggtaagtt cctcgactgg tacgtggaca
     7921 aaagcgaacc gctgcgccgt gctcgcctga tgcatgaccg cgatctgatc atcggctgcg
     7981 cgaaaaaata ccgcgatgtt ggtggcgctg ctgaaggcaa gggggtgctc atcccgttcg
     8041 tgtccggctc gatgcccgac aacgtccacc ggttccggta caaaaattga acaataaccc
     8101 gcttcggcgg gtttttttat ggcttggaac ctcgctggaa cctcgctgga acctcactgg
     8161 aaccttctta aacccgcgcc attgctggtt ggaacctttg gaacctgatt ttctaacctt
     8221 ctctctccaa taacgaaagc tctctcaatt tccctgatta ctctttttaa aaaataggtt
     8281 ccaaggttcc atcgttgatt ttaaaagatt ttttaaggtt ccaaggcggt tccaaggcgg
     8341 ttccacaagg ttccaaggcg gttccagctc aattcctgcg cagttgacgc gccgcagctg
     8401 caccccgttg gggatgcact ttgcgtgtct tggtcgatcg gtggtttggg tggcgctgag
     8461 ggcacgagct gtcgctcgcg ctgggtggtc gtctgttggt tcagcgtgag ggcgtgccct
     8521 cgcgccctct gcgttatgag tggaatgcgt agctgcaggt gcgcgtttgg ttcgtccggg
     8581 catcaaaacg gggcttctcg cgcacgcgag cggtaacaat tgcgtaacgt gcggatccgc
     8641 gtattccgtg ggctcccggg ttttggcggt cacaaaaacg gtcacatggc ggtcatttcg
     8701 gtcatggcgg tcattgttgt ttgcggttca tgccctcgcg catgatgtcg gcctcggtgc
     8761 ccggcttgat ctcgtagatg aggcgttcgc gttcctgcgc tttgagcagg gcggtcatga
     8821 ggccgctgat cgctttgacc atggtggcgt ccttctccag catggcctgt aacgatgcgc
     8881 tgcggagggc ttgcaagtcc tttgcgtggc gctgtagcag gtcaaattgc tgctgtacaa
     8941 agtggttttt aaggcgtcgg atcgcggcct gtggtgtggc ctcgacggcg gcggcggcgc
     9001 gtttccgcat ctcctcgatg ctgaggacgt tgctgggtgc tgcggccttc tcgctcgccc
     9061 atcggcgaac ggtgcggggt gggatcccca tggtgcgtgc gacggtggca ctggttgcgc
     9121 cgtcctgctc cagtagcctg agcgcctggg ctttctgttc ggtggtgtat ttcttcatgg
     9181 ttcctgctcc ggttggtttc tctcccgtat tgaaacgtca cgcgctgaaa atggcggggg
     9241 tttttggggg gtacacggcg tttgtgcgtg cgtggtcgtg cggttgggtg tttttgtggc
     9301 tgaggcgctt agaatggctc tggtgcgttc tggtgaaggg ggagggcgca gctgtcgccg
     9361 cgctcctggt tggggttagc gccagcgttc gagccggtac ctgacagtga tcccgttctc
     9421 cgacatggtg tggacgtagt ccgggttttt cagcgtgagg tcgatcgcct tggtgcggtg
     9481 cgtcttgtat ttataccggg cttcctcggg gaagttctcg cctgtgacca tctcatccca
     9541 tgactcgtat gacttccctc cgggaaccat gatcaccttc catctctgga accggcgctc
     9601 gatgttttca aatgccattt ctctctcctt cgtgttgcta taggtcgata tgcttgcttg
     9661 cggtatctgg cttgcagctc tggctgttca tgctgccgtt gacgacggtg gtaatgatag
     9721 cgatcgggtt gggtgtggtg ttgtacccga cgacgagctg cagtgttgag ctgccctgct
     9781 cgccgttgag gctggcggtt agcttgcctg tctcgatctt ggtgacgggg atgtcggcac
     9841 cgcgccagct ggcggtgagt ctgccattct gctcgttaag gtggataaat gtcccgtcgt
     9901 tgcagatgta ggtcgtggcg actggtgcgg ctgcggcggt tcctgcggtg gcgagaaggg
     9961 ctgcggcgat gatggccttg gttctcatgc tgtgtcctgt gggggcgctc ctcgcgcccc
    10021 gggtgtatgg ttttcgttaa tgcgtaagta aaaccgtatg ctgttttacc ttaacgttta
    10081 agtttttctt acatggctga cggggtgccg atgttctctg ccatccacgc tttgacgcgg
    10141 gggcgcttac cctcctgccg gagtcgtgcg aggacgacgg cgtgctgcag gaagtggcgc
    10201 tctacgtcgg cggtgtcggt gccgagcgcc ttggctatgt cggcggtggc tttcaggatc
    10261 tgcgttctgc ctggggtcat gatcacgcct cccactggaa gagtcggcaa acgggaaggg
    10321 tgacggtgcg cccctctgcc gttctgaggc tgacctggtg gatgcctcgg cggttcatga
    10381 tgacggcgcg tgcgcgtccg tgctgggtga tggtgccgtg ctcgatggat aggcccgggt
    10441 agcgggcagg ggtggtctgt tgtatgctct gcatgtcatt aaacctctaa cgggtggttg
    10501 gtgatgcccg ggttggctct cacacttccc cgggcctggg ttgcggcggt gactttagcg
    10561 agtaccaccg ccgcgatctc tcttacgctt tcgcgtgctt ctgtaaaatc ctgttgaccg
    10621 ttcccctgct gatgccgagg tcgcgtgcga tgcgtgcggc gcttactcct gcgtcgtgct
    10681 tcatgatcac cgctccgacg ttctccggcg tctgtttggt cggtcgtcct gcatggcgtc
    10741 ctgcggctgc ggctgctgcc atccctgcct ttgtgcgctc cttgagggtg gcgagttcca
    10801 tctctgcgaa ggcagcgacc atggtgagca tcatcttccc ggtcggggtg ctgaagtcga
    10861 aaccttcgcg gaggctgatc aggctgacct gccgcttctc cagctcctcg cagacctcca
    10921 tgaccgatct ggcgttgcgt ccgatgcggt cgatcgcgac caccacgatg ctgtcgcctg
    10981 gtgcggcaaa ctccaggatg gctttgaact tgtcgcgctg ggtggcgggt accgttccgc
    11041 tgatgccctc gtcggcgtgg aaggtggctt gtgggtagcg atcgaggatc gctttcttct
    11101 gtccggctac tgtctgttct ccggtgctga cgcgcccgta cccgaatact ctgctcatgc
    11161 tgtctcctgt tgtacaaaaa aggttgtttg gggatatttg accacttatg agcaaaaagc
    11221 aagcgcaaaa aactgtaaac gcctagagcc acgggggctg cgcagtttcg ctcctttttg
    11281 gacgtgatca ccccgcgctg ggcgagagcc ctgcgccctc tgcgttgatg tctgcgctgc
    11341 gtcgtgacgg catgctgtct gagcggcgtt tcttgccgct ttgctgataa cttgctgaga
    11401 accaaaccat gaccgaaatt aagaaagaga gcgccgacga cgcttggcta cgctctaccc
    11461 ttgccgaatt ggcgggtgat gcgttccctg tgtatgacct cccgtccctg caggtagaca
    11521 cgaactcctc cattcagctc tcggcgctcg ccgatcgtca ggctgcgcgt gaaatgcgac
    11581 aggccgcttc cgacgtggaa gcgcgacggc tggacgccgc tcgagtggtg gaagggctca
    11641 agaccgaggc cgaacggctg cggggcttga tcgccgacgg caaggctgcg ctgcgtgctg
    11701 gcgagccggt gagccccgac gcgggtgttg cgtcgttcct gctgccggac atcgaggccg
    11761 agctggttgt tgctgaggct gctgaggccg acgtggcccg ggagcgtgac accctgctgc
    11821 aggatgccga tcgccgtgac gcggcggctg cgctggcgct gttcaactgg gcgcactccg
    11881 ttcgcgtgca gcgtatcgag ctgcttctca agctggcgat ggacgaggcg acgacgctcg
    11941 ctgaagccga cggcgggcgc ggcctgtacc gtaccgtgat cgcgcctgac cgccgtctta
    12001 accaaatttt cgccacgcag ggcgctattg aaatcctgcg ccgtaaccg
//
